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Protein

Vacuolar protease A

Gene

pep-4

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103PROSITE-ProRule annotation1
Active sitei284PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protease A (EC:3.4.23.-)
Gene namesi
Name:pep-4
ORF Names:B13D24.090, NCU02273
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 7, Linkage Group VII

Organism-specific databases

EuPathDBiFungiDB:NCU02273.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002587518 – 70Activation peptideAdd BLAST53
ChainiPRO_000002587671 – 396Vacuolar protease AAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi116 ↔ 121By similarity
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi318 ↔ 351By similarity
Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ01294.

Structurei

3D structure databases

ProteinModelPortaliQ01294.
SMRiQ01294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 392Peptidase A1PROSITE-ProRule annotationAdd BLAST308

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000197681.
InParanoidiQ01294.
KOiK01381.
OMAiWNGQYTV.
OrthoDBiEOG092C33N0.

Family and domain databases

CDDicd05488. Proteinase_A_fungi. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033819. Saccharopepsin.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGALLTAAM LLGSAQAGVH TMKLKKVPLA EQLESVPIDV QVQHLGQKYT
60 70 80 90 100
GLRTESHTQA MFKATDAQVS GNHPVPITNF MNAQYFSEIT IGTPPQTFKV
110 120 130 140 150
VLDTGSSNLW VPSSQCGSIA CYLHNKYESS ESSTYKKNGT SFKIEYGSGS
160 170 180 190 200
LSGFVSQDRM TIGDITINDQ LFAEATSEPG LAFAFGRFDG ILGLGYDRIA
210 220 230 240 250
VNGITPPFYK MVEQKLVDEP VFSFYLADQD GESEVVFGGV NKDRYTGKIT
260 270 280 290 300
TIPLRRKAYW EVDFDAIGYG KDFAELEGHG VILDTGTSLI ALPSQLAEML
310 320 330 340 350
NAQIGAKKSW NGQFTIDCGK KSSLEDVTFT LAGYNFTLGP EDYILEASGS
360 370 380 390
CLSTFMGMDM PAPVGPLAIL GDAFLRKYYS IYDLGADTVG IATAKR
Length:396
Mass (Da):42,933
Last modified:February 15, 2005 - v2
Checksum:i3498032A1E703195
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31 – 32EQ → DE in AAA79878 (PubMed:8702999).Curated2
Sequence conflicti199I → L in AAA79878 (PubMed:8702999).Curated1
Sequence conflicti202N → P in AAA79878 (PubMed:8702999).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36471 Genomic DNA. Translation: AAA79878.1.
BX908789 Genomic DNA. Translation: CAF05874.1.
CM002242 Genomic DNA. Translation: EAA30681.1.
PIRiT47207.
RefSeqiXP_959917.1. XM_954824.3.

Genome annotation databases

EnsemblFungiiEAA30681; EAA30681; NCU02273.
GeneIDi3876039.
KEGGincr:NCU02273.

Similar proteinsi

Entry informationi

Entry nameiCARP_NEUCR
AccessioniPrimary (citable) accession number: Q01294
Secondary accession number(s): Q7RVB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 15, 2005
Last modified: July 5, 2017
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families