Reviewed, UniProtKB/Swiss-Prot Q01289 (POR_PEA)

Names and origin

Protein names

Recommended name:
    Protochlorophyllide reductase, chloroplastic
      Short name=PCR
    EC=1.3.1.33
Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase
      Short name=POR

Gene names

Name:

3PCR

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP(+) = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: InterPro
Molecular function	binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Chloroplast
	Chain	65 – 399	335	Protochlorophyllide reductase, chloroplastic		PRO_0000023296

Sequences

	Sequence		Length	Mass (Da)
	Q01289-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E6607FB2CD4837D2	FASTA	399	42,962
	10 20 30 40 50 60 MALQTASMLP ASFSIPKEGK IGASLKDSTL FGVSSLSDSL KGDFTSSALR CKELRQKVGA 70 80 90 100 110 120 VRAETAAPAT PAVNKSSSEG KKTLRKGNVV ITGASSGLGL ATAKALAESG KWHVIMACRD 130 140 150 160 170 180 YLKAARAAKS AGLAKENYTI MHLDLASLDS VRQFVDNFRR SEMPLDVLIN NAAVYFPTAK 190 200 210 220 230 240 EPSFTADGFE ISVGTNHLGH FLLSRLLLED LKKSDYPSKR LIIVGSITGN TNTLAGNVPP 250 260 270 280 290 300 KANLGDLRGL AGGLTGLNSS AMIDGGDFDG AKAYKDSKVC NMLTMQEFHR RYHEETGITF 310 320 330 340 350 360 ASLYPGCIAT TGLFREHIPL FRTLFPPFQK YITKGYVSEE ESGKRLAQVV SDPSLTKSGV 370 380 390 YWSWNNASAS FENQLSQEAS DAEKARKVWE VSEKLVGLA

References

[1]

"Molecular cloning, nuclear gene structure, and developmental expression of NADPH: protochlorophyllide oxidoreductase in pea (Pisum sativum L.)." Spano A.J., He Z., Michel H., Hunt D.F., Timko M.P. Plant Mol. Biol. 18:967-972(1992) [PubMed: 1581573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 65-76. Strain: cv. Progress No. 9.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63060 Genomic DNA. Translation: CAA44786.1.
PIR	S20941.
3D structure databases
HSSP	HSSP built from PDB template 1FDU based on UniProtKB P14061.
ModBase	Search...
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. IPR005979. Prochl_reduct. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

POR_PEA

Accession

Primary (citable) accession number: Q01289

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)