Reviewed, UniProtKB/Swiss-Prot Q01285 (CHS4_NEUCR)

Names and origin

Protein names

Recommended name:
    Chitin synthase 4
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 4
    Class-IV chitin synthase 4

Gene names

Name:	chs-4
ORF Names:	NCU09324

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1235	1235	Chitin synthase 4		PRO_0000193704
Regions
	Transmembrane	213 – 233	21	Potential
	Transmembrane	245 – 265	21	Potential
	Transmembrane	515 – 535	21	Potential
	Transmembrane	1066 – 1086	21	Potential
	Transmembrane	1093 – 1113	21	Potential
	Transmembrane	1117 – 1137	21	Potential
	Compositional bias	4 – 87	84	Pro-rich
Experimental info
	Sequence conflict	480	1	D → Y in AAB03563. Ref.1
	Sequence conflict	563	1	D → E in AAB03563. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01285-1 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: F275C6603FB8CDD4	FASTA	1,235	137,990
	10 20 30 40 50 60 MSLPRRPGPS PPHEEPRRYR QSGSRRSRPP PADVETGYAP MAGERPSQQQ RVPSISSFPE 70 80 90 100 110 120 TLPSPNPNVE RDPLAPTPEP AHPSGIPQRK RSLIRPERNR IGKDHPNYHY RKHAANMNTL 130 140 150 160 170 180 PSSTGHDPIY EDLEGATDDV SGTGSRNDDD VSEESPPRRK HSTKMQVIET EKSGDERRRR 190 200 210 220 230 240 RKSDTTKHGK IVKASKGKRE KSGGLPTPSF WNIYCGFVTF WCPGFVLKCF GMPEMAQQRA 250 260 270 280 290 300 WREKMGLISI ILLIMGFVGF ITFGFTQVVC GKPPLRLRIN EVGSGYMIFH GSAYDLTKSH 310 320 330 340 350 360 HPPAEGIPRR PDGLGANVIY DLPQHYGGQD GSFLFQNVNG KCKGLITKQE NSDVPSDKSG 370 380 390 400 410 420 NLAWYFPCNT FNQDGSSKPN TTIPYYLGYA CHTTANARDS FYLGLKSSAD VYFTWDDIKN 430 440 450 460 470 480 SSRNLVVYSG HVLDLDLLHW FNDTQVTYPA RFKELRDKNT AGNQAIRGRD ITHAFQSSKD 490 500 510 520 530 540 KQIAECFEEI IKVGSVDTET VGCIASKVVL YVSLVLILAV VLARFVLALI FQWFISKTYA 550 560 570 580 590 600 AAKTSQTSDQ RKRNRQIEDW TEDIYRAPPR LPGEVGSSVA GSSDRQSKRS SAFLPTHSRF 610 620 630 640 650 660 STVYGNERGN RKPGLPTTMA SQNAAGQLLH PGTIYGQGNE SRSSFLKSDA YGSSSSPADG 670 680 690 700 710 720 PGPAGFIHEA VVPQPPSDWM PFGFPLAHTI CLVTAYSEGE MGVRTTLDSI AMTDYPNSHK 730 740 750 760 770 780 VILVICDGII KGHGEEHSTP DIILGMMKDH TIHPDDVEPF SYVAVATGSK RHNMAKVYTG 790 800 810 820 830 840 FYDYGTNSAI PLEKQQRVPM MMVVKCGTPA EASKSKPGNR GKRDSQIILM SFLQKVMFDE 850 860 870 880 890 900 RMTELEYEMF NGLWKITGIS PDFYEIVLMV DADTKVFPDS LTHMISAMVK DPEIMGLCGE 910 920 930 940 950 960 TKIANKRASW VSAIQVFEYF VSHHLAKAFE SVFGGVTCLP GCFCMYRIKA PKGAQNYWVP 970 980 990 1000 1010 1020 ILANPDVVEH YSENVVDTLH KKNLLLLGED RYLSTLMLRT FPKRKQVFVP QAVCKTTVPD 1030 1040 1050 1060 1070 1080 EFMVLLSQRR RWINSTIHNL MELVLVRDLC GTFCFSMQFI VGIELIGTLV LPAAIAFTFY 1090 1100 1110 1120 1130 1140 VVIISIINSP PQIIPLVLLG LILGLPAILV VVTAHSWSYI IWMFIYLLSL PVWNFVLPTY 1150 1160 1170 1180 1190 1200 AFWKFDDFSW GDTRKTAGEK SSKGGHGEAE GEFDSSMITM KRWAEFERDR RVRSTYWAGS 1210 1220 1230 RDNVISGVGG SNGWGSSQPR GHEQGRHFDD YFSDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"chs-4, a class IV chitin synthase gene from Neurospora crassa." Din A.B., Specht C.A., Robbins P.W., Yarden O. Mol. Gen. Genet. 250:214-222(1996) [PubMed: 8628221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U25097 Genomic DNA. Translation: AAB03563.1. Different initiation. AABX02000072 Genomic DNA. Translation: EAA34792.2.
PIR	S61886.
RefSeq	XP_964028.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q01285.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	3880177.
KEGG	ncr:NCU09324.
NMPDR	fig|5141.1.peg.9949.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-000529-MON.
BRENDA	2.4.1.16. 266.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. [Graphical view]
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS4_NEUCR

Accession

Primary (citable) accession number: Q01285
Secondary accession number(s): Q7RVJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 4, 2007
Last modified:	October 13, 2009
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)