Reviewed, UniProtKB/Swiss-Prot Q01284 (2NPD_NEUCR)

Names and origin

Protein names

Recommended name:
    2-nitropropane dioxygenase
      Short name=2-NPD
    EC=1.13.11.32
Alternative name(s):
    Nitroalkane oxidase

Gene names

Name:	ncd-2
ORF Names:	G17A4.200, NCU03949

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of nitroalkanes to produce the corresponding carbonyl compounds. It acts on 2-nitropropane better than on nitroethane and 1-nitropropane, and anionic forms of nitroalkanes are much better substrates than are neutral forms.
Catalytic activity	2 2-nitropropane + O2 = 2 acetone + 2 nitrite.
Cofactor	FMN.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the 2-nitropropane dioxygenase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-nitropropane dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 15	15	Potential		PRO_0000020575
	Chain	16 – 378	363	2-nitropropane dioxygenase		PRO_0000020576

Sequences

	Sequence		Length	Mass (Da)
	Q01284-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E453EB43FD23E441	FASTA	378	39,916
	10 20 30 40 50 60 MHFPGHSSKK EESAQAALTK LNSWFPTTKN PVIISAPMYL IANGTLAAEV SKAGGIGFVA 70 80 90 100 110 120 GGSDFRPGSS HLTALSTELA SARSRLGLTD RPLTPLPGIG VGLILTHTIS VPYVTDTVLP 130 140 150 160 170 180 ILIEHSPQAV WLFANDPDFE ASSEPGAKGT AKQIIEALHA SGFVVFFQVG TVKDARKAAA 190 200 210 220 230 240 DGADVIVAQG IDAGGHQLAT GSGIVSLVPE VRDMLDREFK EREVVVVAAG GVADGRGVVG 250 260 270 280 290 300 ALGLGAEGVV LGTRFTVAVE ASTPEFRRKV ILETNDGGLN TVKSHFHDQI NCNTIWHNVY 310 320 330 340 350 360 DGRAVRNASY DDHAAGVPFE ENHKKFKEAA SSGDNSRAVT WSGTAVGLIK DQRPAGDIVR 370 ELREEAKERI KKIQAFAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa." Gorlatova N., Tchorzewski M., Kurihara T., Soda K., Esaki N. Appl. Environ. Microbiol. 64:1029-1033(1998) [PubMed: 9501443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION. Strain: ATCC 10337 / IFO 6067 / FGSC 1758 / IMI 53239.
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U22530 mRNA. Translation: AAA64218.1. BX908812 Genomic DNA. Translation: CAF06155.1. AABX02000012 Genomic DNA. Translation: EAA28352.1.
PIR	T46693.
RefSeq	XP_957588.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3873678.
KEGG	ncr:NCU03949.
NMPDR	fig|5141.1.peg.1201.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-302. NCRA-XX3-01:NCRA-XX3-01-007697-MON.
BRENDA	1.13.11.32. 266.
Family and domain databases
InterPro	IPR004136. 2Npropane_dOase. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF03060. NPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

2NPD_NEUCR

Accession

Primary (citable) accession number: Q01284
Secondary accession number(s): Q7RV78

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)