Reviewed, UniProtKB/Swiss-Prot Q01269 (PHEC_PSEAE)

Names and origin

Protein names

Recommended name:
    Cyclohexadienyl dehydratase
Including the following 2 domains:
    1- Recommended name:
            Prephenate dehydratase
              EC=4.2.1.51
    2- Recommended name:
            Arogenate dehydratase
              EC=4.2.1.91

Gene names

Name:	pheC
Ordered Locus Names:	PA3475

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms alternative pathway for phenylalanine biosynthesis. Can catalyze two reactions: prephenate dehydratase and arogenate dehydratase. May have a role in chemotaxis or transport.
Catalytic activity	Prephenate = phenylpyruvate + H2O + CO2. L-arogenate = L-phenylalanine + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from L-arogenate: step 1/1. Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Subunit structure	Homodimer Probable.
Subcellular location	Periplasm. Ref.3
Sequence similarities	Belongs to the bacterial solute-binding protein 3 family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	arogenate dehydratase activity Inferred from electronic annotation. Source: EC prephenate dehydratase activity Inferred from electronic annotation. Source: EC transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.3
	Chain	26 – 268	243	Cyclohexadienyl dehydratase		PRO_0000031773
Sites
	Site	179	1	Important for catalysis
Experimental info
	Sequence conflict	42	1	A → T in AAC08596. Ref.1
	Sequence conflict	115	1	Y → H in AAC08596. Ref.1
	Sequence conflict	123 – 124	2	DG → NS in AAC08596. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01269-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 74E7AA47473DDF4B	FASTA	268	30,448
	10 20 30 40 50 60 MPKSFRHLVQ ALACLALLAS ASLQAQESRL DRILESGVLR VATTGDYKPF SYRTEEGGYA 70 80 90 100 110 120 GFDVDMAQRL AESLGAKLVV VPTSWPNLMR DFADDRFDIA MSGISINLER QRQAYFSIPY 130 140 150 160 170 180 LRDGKTPITL CSEEARFQTL EQIDQPGVTA IVNPGGTNEK FARANLKKAR ILVHPDNVTI 190 200 210 220 230 240 FQQIVDGKAD LMMTDAIEAR LQSRLHPELC AVHPQQPFDF AEKAYLLPRD EAFKRYVDQW 250 260 LHIAEQSGLL RQRMEHWLEY RWPTAHGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product." Zhao G., Xia T.H., Fischer R.S., Jensen R.A. J. Biol. Chem. 267:2487-2493(1992) [PubMed: 1733946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Cyclohexadienyl dehydratase from Pseudomonas aeruginosa is a periplasmic protein." Zhao G., Xia T., Aldrich H., Jensen R.A. J. Gen. Microbiol. 139:807-813(1993) [PubMed: 8515238] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-36, SUBCELLULAR LOCATION.
[4]	"Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa." Ochsner U.A., Reiser J. Proc. Natl. Acad. Sci. U.S.A. 92:6424-6428(1995) [PubMed: 7604006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7. Strain: DSM 2659 / PG201.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF054868 Genomic DNA. Translation: AAC08596.1. AE004091 Genomic DNA. Translation: AAG06863.1.
PIR	B42325. H83211.
RefSeq	NP_252165.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878976.
GenomeReviews	Gene locus PA3475 in contig AE004091_GR.
KEGG	pae:PA3475.
Organism-specific databases
PseudoCAP	PA3475.
CMR	Search...
Phylogenomic databases
HOGENOM	Q01269.
OMA	LHPELCA.
Enzyme and pathway databases
BioCyc	PAER208964:PA3475-MON.
BRENDA	4.2.1.51. 354. 4.2.1.91. 354.
Family and domain databases
InterPro	IPR015683. Glutamate_receptor-rel. IPR001638. SBP_bac_3. IPR018313. SBP_bac_3_CS. [Graphical view]
PANTHER	PTHR18966. Glut_Rec_Related. 1 hit.
Pfam	PF00497. SBP_bac_3. 1 hit. [Graphical view]
SMART	SM00062. PBPb. 1 hit. [Graphical view]
PROSITE	PS01039. SBP_BACTERIAL_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEC_PSEAE

Accession

Primary (citable) accession number: Q01269
Secondary accession number(s): Q9HYD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)