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Q01260

- GP_INSV

UniProt

Q01260 - GP_INSV

Protein

Envelope glycoprotein

Gene

GP

Organism
Impatiens necrotic spot virus (INSV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei459 – 4602Cleavage; by host signal peptidaseBy similarity

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. modulation by virus of host morphology or physiology Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Short name:
    GP
    Alternative name(s):
    M polyprotein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:GP
    OrganismiImpatiens necrotic spot virus (INSV)
    Taxonomic identifieri11612 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
    Virus hostiImpatiens [TaxID: 35939]

    Subcellular locationi

    Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.By similarity
    Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 88Sequence AnalysisPRO_0000036868
    Chaini9 – 11101102Envelope glycoproteinPRO_0000036869Add
    BLAST
    Chaini9 – 459451Glycoprotein G1Sequence AnalysisPRO_0000036870Add
    BLAST
    Chaini460 – 1110651Glycoprotein G2Sequence AnalysisPRO_0000036871Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi46 – 461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi186 – 1861N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi566 – 5661N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi582 – 5821N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi957 – 9571N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.By similarity
    Glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    G1 and G2 interacts with each other.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 321321LumenalSequence AnalysisAdd
    BLAST
    Topological domaini343 – 459117CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini460 – 1044585LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1066 – 111045CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei322 – 34221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1045 – 106521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi14 – 163Cell attachment siteSequence Analysis

    Domaini

    The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view]
    PfamiPF03557. Bunya_G1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01260-1 [UniParc]FASTAAdd to Basket

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    MALKETDAKI HVERGDHPEI YDEAYYDRSV DHKNEILDTL AEMLQNATGK     50
    TLRPTRDTQT VLANNEVPQS SSGLSSTPTT ISIMDLPNPC LNASSLTCSI 100
    KGVSTFNVYY QVESNGVIYS CISDTITKLG NCEGSSELPR SFETVPVVPI 150
    TKIDNKRKLS IGTKFYIIES LENYNYPIMY NSRPTNGTVS LQSVKFSGDC 200
    KISKTNIVNS YTVSLTTPEK IMGYVVKREG SDMSHSIISF SGSVSLTFTE 250
    ENMDGKHNLL CGDKSSKVPL VDKRVRDCII KYSKNIYKQT ACINFSWFRL 300
    IMIALIVYFP IRYLVNKTSK TLFYGYDLLG LITYPILLLI NYLWSYFPLK 350
    CKVCGNLCLV THECSKLCIC NKNKASEEHS EECPIITRTA EKNKKYNWAS 400
    IEWFHLIVNT KIGLSFLKAV TETLIGFLIL SQMPMSMAQT AQCLDSCYYV 450
    PGCDRFVTNR YDKCPEKDQC FCAIKENSIV ESNFLTNVVT EGPMDCIPYQ 500
    ECKGRITENA LVTFVKCRFG CEYASIFQSK PLDNGFLEYS GDTLGLNAVN 550
    LHFMKRLRNG IIDFYNKTEK YGYISGDALK SNESDIPESI FPRKSLIFDS 600
    VIDGKYRYMI EESLLSGGGT VFSLNDKSSS TAQKFVVYIK KVRIQYDVSE 650
    QYTTAPIQST HTDFFSTCTG KCSDCRKEQP ITGYQDFCIT PTSYWGCEEV 700
    WCLAINEGAT CGFCRNVYDM DQSFRIYSVI KSTIKSEVCI SGFVGAKCFT 750
    VSEEVPSESG YFQADILADF HNDGLTIGQL IAHGPDSHVY AGNIARLNNP 800
    SKMFGHPQLS HQGDPIFSKK TLDTNDLSWD CSAIGKKTIT IKSCGYDTYR 850
    FKTGLNQISD IPVQFTDQNS FYMEKIFSLG KLKIVLDLPS ELFKTVPKKP 900
    ILSSVSLSCK GCFLCSQGLR CAASFISDIT FSARLTMKQC SLSTYQIAVK 950
    KGANKYNLTM FCTSNPEKQK MIIEPEGDKS YSVEALVDSV AVLEPENIID 1000
    QNDQHAHEEQ QYNSDTSVWS FWDYVKSPFN FIASHFGSFF DTVRVVLLIL 1050
    FVFALAYLCS IVATMCRGYV RNKSYKTKYI EDTNDYSLVS TSSGKDTITR 1100
    RRPPLDFSGI 1110
    Length:1,110
    Mass (Da):124,868
    Last modified:February 1, 1994 - v1
    Checksum:iB0691F892BAC7F6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74904 Genomic RNA. Translation: AAA46242.1.
    PIRiB42544.
    RefSeqiNP_619691.1. NC_003616.1.

    Genome annotation databases

    GeneIDi956573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74904 Genomic RNA. Translation: AAA46242.1 .
    PIRi B42544.
    RefSeqi NP_619691.1. NC_003616.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956573.

    Family and domain databases

    InterProi IPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view ]
    Pfami PF03557. Bunya_G1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization."
      Law M.D., Speck J., Moyer J.W.
      Virology 188:732-741(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiGP_INSV
    AccessioniPrimary (citable) accession number: Q01260
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3