Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q01260

- GP_INSV

UniProt

Q01260 - GP_INSV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Envelope glycoprotein

Gene

GP

Organism
Impatiens necrotic spot virus (INSV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei459 – 4602Cleavage; by host signal peptidaseBy similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. modulation by virus of host morphology or physiology Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiImpatiens necrotic spot virus (INSV)
Taxonomic identifieri11612 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostiImpatiens [TaxID: 35939]

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs (By similarity).By similarity
Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 321321LumenalSequence AnalysisAdd
BLAST
Transmembranei322 – 34221HelicalSequence AnalysisAdd
BLAST
Topological domaini343 – 459117CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini460 – 1044585LumenalSequence AnalysisAdd
BLAST
Transmembranei1045 – 106521HelicalSequence AnalysisAdd
BLAST
Topological domaini1066 – 111045CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 88Sequence AnalysisPRO_0000036868
Chaini9 – 11101102Envelope glycoproteinPRO_0000036869Add
BLAST
Chaini9 – 459451Glycoprotein G1Sequence AnalysisPRO_0000036870Add
BLAST
Chaini460 – 1110651Glycoprotein G2Sequence AnalysisPRO_0000036871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi92 – 921N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi186 – 1861N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi566 – 5661N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi582 – 5821N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi957 – 9571N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.By similarity
Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.By similarity

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi14 – 163Cell attachment siteSequence Analysis

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01260-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKETDAKI HVERGDHPEI YDEAYYDRSV DHKNEILDTL AEMLQNATGK
60 70 80 90 100
TLRPTRDTQT VLANNEVPQS SSGLSSTPTT ISIMDLPNPC LNASSLTCSI
110 120 130 140 150
KGVSTFNVYY QVESNGVIYS CISDTITKLG NCEGSSELPR SFETVPVVPI
160 170 180 190 200
TKIDNKRKLS IGTKFYIIES LENYNYPIMY NSRPTNGTVS LQSVKFSGDC
210 220 230 240 250
KISKTNIVNS YTVSLTTPEK IMGYVVKREG SDMSHSIISF SGSVSLTFTE
260 270 280 290 300
ENMDGKHNLL CGDKSSKVPL VDKRVRDCII KYSKNIYKQT ACINFSWFRL
310 320 330 340 350
IMIALIVYFP IRYLVNKTSK TLFYGYDLLG LITYPILLLI NYLWSYFPLK
360 370 380 390 400
CKVCGNLCLV THECSKLCIC NKNKASEEHS EECPIITRTA EKNKKYNWAS
410 420 430 440 450
IEWFHLIVNT KIGLSFLKAV TETLIGFLIL SQMPMSMAQT AQCLDSCYYV
460 470 480 490 500
PGCDRFVTNR YDKCPEKDQC FCAIKENSIV ESNFLTNVVT EGPMDCIPYQ
510 520 530 540 550
ECKGRITENA LVTFVKCRFG CEYASIFQSK PLDNGFLEYS GDTLGLNAVN
560 570 580 590 600
LHFMKRLRNG IIDFYNKTEK YGYISGDALK SNESDIPESI FPRKSLIFDS
610 620 630 640 650
VIDGKYRYMI EESLLSGGGT VFSLNDKSSS TAQKFVVYIK KVRIQYDVSE
660 670 680 690 700
QYTTAPIQST HTDFFSTCTG KCSDCRKEQP ITGYQDFCIT PTSYWGCEEV
710 720 730 740 750
WCLAINEGAT CGFCRNVYDM DQSFRIYSVI KSTIKSEVCI SGFVGAKCFT
760 770 780 790 800
VSEEVPSESG YFQADILADF HNDGLTIGQL IAHGPDSHVY AGNIARLNNP
810 820 830 840 850
SKMFGHPQLS HQGDPIFSKK TLDTNDLSWD CSAIGKKTIT IKSCGYDTYR
860 870 880 890 900
FKTGLNQISD IPVQFTDQNS FYMEKIFSLG KLKIVLDLPS ELFKTVPKKP
910 920 930 940 950
ILSSVSLSCK GCFLCSQGLR CAASFISDIT FSARLTMKQC SLSTYQIAVK
960 970 980 990 1000
KGANKYNLTM FCTSNPEKQK MIIEPEGDKS YSVEALVDSV AVLEPENIID
1010 1020 1030 1040 1050
QNDQHAHEEQ QYNSDTSVWS FWDYVKSPFN FIASHFGSFF DTVRVVLLIL
1060 1070 1080 1090 1100
FVFALAYLCS IVATMCRGYV RNKSYKTKYI EDTNDYSLVS TSSGKDTITR
1110
RRPPLDFSGI
Length:1,110
Mass (Da):124,868
Last modified:February 1, 1994 - v1
Checksum:iB0691F892BAC7F6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74904 Genomic RNA. Translation: AAA46242.1.
PIRiB42544.
RefSeqiNP_619691.1. NC_003616.1.

Genome annotation databases

GeneIDi956573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74904 Genomic RNA. Translation: AAA46242.1 .
PIRi B42544.
RefSeqi NP_619691.1. NC_003616.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956573.

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
[Graphical view ]
PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization."
    Law M.D., Speck J., Moyer J.W.
    Virology 188:732-741(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiGP_INSV
AccessioniPrimary (citable) accession number: Q01260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3