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Protein

Envelopment polyprotein

Gene

GP

Organism
Impatiens necrotic spot virus (INSV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Glycoprotein N: Together with Glycoprotein C are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity
Glycoprotein C: Together with Glycoprotein N are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiImpatiens necrotic spot virus (INSV)
Taxonomic identifieri11612 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostiImpatiens [TaxID: 35939]

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Glycoprotein C alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of Glycoprotein C and Glycoprotein N results in efficient transport of Glycoprotein C to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 321LumenalSequence analysisAdd BLAST321
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 459CytoplasmicSequence analysisAdd BLAST117
Topological domaini460 – 1044LumenalSequence analysisAdd BLAST585
Transmembranei1045 – 1065HelicalSequence analysisAdd BLAST21
Topological domaini1066 – 1110CytoplasmicSequence analysisAdd BLAST45

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000368681 – 8Sequence analysis8
ChainiPRO_00000368699 – 1110Envelopment polyproteinAdd BLAST1102
ChainiPRO_00000368709 – 459Glycoprotein NSequence analysisAdd BLAST451
ChainiPRO_0000036871460 – 1110Glycoprotein CSequence analysisAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi92N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi186N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi316N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi566N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi582N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi957N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including Glycoprotein N and Glycoprotein C.By similarity
Glycosylated. Glycosylation is essential for proper subcellular location.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei459 – 460Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Glycoprotein N: Interacts with Glycoprotein C and nucleoprotein. Glycoprotein C: Interacts with Glycoprotein N and nucleoprotein.By similarity

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 16Cell attachment siteSequence analysis3

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKETDAKI HVERGDHPEI YDEAYYDRSV DHKNEILDTL AEMLQNATGK
60 70 80 90 100
TLRPTRDTQT VLANNEVPQS SSGLSSTPTT ISIMDLPNPC LNASSLTCSI
110 120 130 140 150
KGVSTFNVYY QVESNGVIYS CISDTITKLG NCEGSSELPR SFETVPVVPI
160 170 180 190 200
TKIDNKRKLS IGTKFYIIES LENYNYPIMY NSRPTNGTVS LQSVKFSGDC
210 220 230 240 250
KISKTNIVNS YTVSLTTPEK IMGYVVKREG SDMSHSIISF SGSVSLTFTE
260 270 280 290 300
ENMDGKHNLL CGDKSSKVPL VDKRVRDCII KYSKNIYKQT ACINFSWFRL
310 320 330 340 350
IMIALIVYFP IRYLVNKTSK TLFYGYDLLG LITYPILLLI NYLWSYFPLK
360 370 380 390 400
CKVCGNLCLV THECSKLCIC NKNKASEEHS EECPIITRTA EKNKKYNWAS
410 420 430 440 450
IEWFHLIVNT KIGLSFLKAV TETLIGFLIL SQMPMSMAQT AQCLDSCYYV
460 470 480 490 500
PGCDRFVTNR YDKCPEKDQC FCAIKENSIV ESNFLTNVVT EGPMDCIPYQ
510 520 530 540 550
ECKGRITENA LVTFVKCRFG CEYASIFQSK PLDNGFLEYS GDTLGLNAVN
560 570 580 590 600
LHFMKRLRNG IIDFYNKTEK YGYISGDALK SNESDIPESI FPRKSLIFDS
610 620 630 640 650
VIDGKYRYMI EESLLSGGGT VFSLNDKSSS TAQKFVVYIK KVRIQYDVSE
660 670 680 690 700
QYTTAPIQST HTDFFSTCTG KCSDCRKEQP ITGYQDFCIT PTSYWGCEEV
710 720 730 740 750
WCLAINEGAT CGFCRNVYDM DQSFRIYSVI KSTIKSEVCI SGFVGAKCFT
760 770 780 790 800
VSEEVPSESG YFQADILADF HNDGLTIGQL IAHGPDSHVY AGNIARLNNP
810 820 830 840 850
SKMFGHPQLS HQGDPIFSKK TLDTNDLSWD CSAIGKKTIT IKSCGYDTYR
860 870 880 890 900
FKTGLNQISD IPVQFTDQNS FYMEKIFSLG KLKIVLDLPS ELFKTVPKKP
910 920 930 940 950
ILSSVSLSCK GCFLCSQGLR CAASFISDIT FSARLTMKQC SLSTYQIAVK
960 970 980 990 1000
KGANKYNLTM FCTSNPEKQK MIIEPEGDKS YSVEALVDSV AVLEPENIID
1010 1020 1030 1040 1050
QNDQHAHEEQ QYNSDTSVWS FWDYVKSPFN FIASHFGSFF DTVRVVLLIL
1060 1070 1080 1090 1100
FVFALAYLCS IVATMCRGYV RNKSYKTKYI EDTNDYSLVS TSSGKDTITR
1110
RRPPLDFSGI
Length:1,110
Mass (Da):124,868
Last modified:February 1, 1994 - v1
Checksum:iB0691F892BAC7F6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74904 Genomic RNA. Translation: AAA46242.1.
PIRiB42544.
RefSeqiNP_619691.1. NC_003616.1.

Genome annotation databases

GeneIDi956573.
KEGGivg:956573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74904 Genomic RNA. Translation: AAA46242.1.
PIRiB42544.
RefSeqiNP_619691.1. NC_003616.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi956573.
KEGGivg:956573.

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGP_INSV
AccessioniPrimary (citable) accession number: Q01260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 20, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.