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Q01260 (GP_INSV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

  1. Glycoprotein G1
  2. Glycoprotein G2
Gene names
Name:GP
OrganismImpatiens necrotic spot virus (INSV)
Taxonomic identifier11612 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostImpatiens [TaxID: 35939]

Protein attributes

Sequence length1110 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal By similarity.

Domain

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2 By similarity.

Glycosylated By similarity.

Sequence similarities

Belongs to the tospovirus envelope glycoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 88 Potential
PRO_0000036868
Chain9 – 11101102Envelope glycoprotein
PRO_0000036869
Chain9 – 459451Glycoprotein G1 Potential
PRO_0000036870
Chain460 – 1110651Glycoprotein G2 Potential
PRO_0000036871

Regions

Topological domain1 – 321321Lumenal Potential
Transmembrane322 – 34221Helical; Potential
Topological domain343 – 459117Cytoplasmic Potential
Topological domain460 – 1044585Lumenal Potential
Transmembrane1045 – 106521Helical; Potential
Topological domain1066 – 111045Cytoplasmic Potential
Motif14 – 163Cell attachment site Potential

Sites

Site459 – 4602Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation461N-linked (GlcNAc...); by host Potential
Glycosylation921N-linked (GlcNAc...); by host Potential
Glycosylation1861N-linked (GlcNAc...); by host Potential
Glycosylation3161N-linked (GlcNAc...); by host Potential
Glycosylation5661N-linked (GlcNAc...); by host Potential
Glycosylation5821N-linked (GlcNAc...); by host Potential
Glycosylation9571N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q01260 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B0691F892BAC7F6F

FASTA1,110124,868
        10         20         30         40         50         60 
MALKETDAKI HVERGDHPEI YDEAYYDRSV DHKNEILDTL AEMLQNATGK TLRPTRDTQT 

        70         80         90        100        110        120 
VLANNEVPQS SSGLSSTPTT ISIMDLPNPC LNASSLTCSI KGVSTFNVYY QVESNGVIYS 

       130        140        150        160        170        180 
CISDTITKLG NCEGSSELPR SFETVPVVPI TKIDNKRKLS IGTKFYIIES LENYNYPIMY 

       190        200        210        220        230        240 
NSRPTNGTVS LQSVKFSGDC KISKTNIVNS YTVSLTTPEK IMGYVVKREG SDMSHSIISF 

       250        260        270        280        290        300 
SGSVSLTFTE ENMDGKHNLL CGDKSSKVPL VDKRVRDCII KYSKNIYKQT ACINFSWFRL 

       310        320        330        340        350        360 
IMIALIVYFP IRYLVNKTSK TLFYGYDLLG LITYPILLLI NYLWSYFPLK CKVCGNLCLV 

       370        380        390        400        410        420 
THECSKLCIC NKNKASEEHS EECPIITRTA EKNKKYNWAS IEWFHLIVNT KIGLSFLKAV 

       430        440        450        460        470        480 
TETLIGFLIL SQMPMSMAQT AQCLDSCYYV PGCDRFVTNR YDKCPEKDQC FCAIKENSIV 

       490        500        510        520        530        540 
ESNFLTNVVT EGPMDCIPYQ ECKGRITENA LVTFVKCRFG CEYASIFQSK PLDNGFLEYS 

       550        560        570        580        590        600 
GDTLGLNAVN LHFMKRLRNG IIDFYNKTEK YGYISGDALK SNESDIPESI FPRKSLIFDS 

       610        620        630        640        650        660 
VIDGKYRYMI EESLLSGGGT VFSLNDKSSS TAQKFVVYIK KVRIQYDVSE QYTTAPIQST 

       670        680        690        700        710        720 
HTDFFSTCTG KCSDCRKEQP ITGYQDFCIT PTSYWGCEEV WCLAINEGAT CGFCRNVYDM 

       730        740        750        760        770        780 
DQSFRIYSVI KSTIKSEVCI SGFVGAKCFT VSEEVPSESG YFQADILADF HNDGLTIGQL 

       790        800        810        820        830        840 
IAHGPDSHVY AGNIARLNNP SKMFGHPQLS HQGDPIFSKK TLDTNDLSWD CSAIGKKTIT 

       850        860        870        880        890        900 
IKSCGYDTYR FKTGLNQISD IPVQFTDQNS FYMEKIFSLG KLKIVLDLPS ELFKTVPKKP 

       910        920        930        940        950        960 
ILSSVSLSCK GCFLCSQGLR CAASFISDIT FSARLTMKQC SLSTYQIAVK KGANKYNLTM 

       970        980        990       1000       1010       1020 
FCTSNPEKQK MIIEPEGDKS YSVEALVDSV AVLEPENIID QNDQHAHEEQ QYNSDTSVWS 

      1030       1040       1050       1060       1070       1080 
FWDYVKSPFN FIASHFGSFF DTVRVVLLIL FVFALAYLCS IVATMCRGYV RNKSYKTKYI 

      1090       1100       1110 
EDTNDYSLVS TSSGKDTITR RRPPLDFSGI 

« Hide

References

[1]"The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization."
Law M.D., Speck J., Moyer J.W.
Virology 188:732-741(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74904 Genomic RNA. Translation: AAA46242.1.
PIRB42544.
RefSeqNP_619691.1. NC_003616.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956573.

Family and domain databases

InterProIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFPIRSF003960. M_poly_TospoV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGP_INSV
AccessionPrimary (citable) accession number: Q01260
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families