Reviewed, UniProtKB/Swiss-Prot Q01257 (ARSC_STAXY)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:

arsC

Encoded on

Plasmid pSX267

Organism

Staphylococcus xylosus

Taxonomic identifier

1288 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity. HAMAP MF_01624
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity. HAMAP MF_01624
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Protein arsC HAMAP MF_01624		PRO_0000162534
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	82	1	Nucleophile; for reductase activity By similarity
	Active site	89	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 82		Redox-active; alternate By similarity
	Disulfide bond	82 ↔ 89		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01257-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 99C84FA73896D094	FASTA	131	14,888
	10 20 30 40 50 60 MDKKTIYFIC TGNSCRSQMA EGWGREILGE DWNVYSAGIE THGVNPKAIE AMKEVDIDIS 70 80 90 100 110 120 NHTSDLIDNH ILKQSDLVVT LCSDADDNCP ILPPNVKKEH WGLEDPAGKE WSEFQRVRDE 130 IKLAIENFKL R

References

[1]

"Expression and regulation of the antimonite, arsenite, and arsenate resistance operon of Staphylococcus xylosus plasmid pSX267." Rosenstein R., Peschel A., Wieland B., Goetz F. J. Bacteriol. 174:3676-3683(1992) [PubMed: 1534327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20267 / Isolate C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80565 Genomic DNA. Translation: AAA27589.1.
PIR	C41902.
3D structure databases
SMR	Q01257. Positions 2-131.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.48. 141621.
Family and domain databases
HAMAP	MF_01624. Arsenate_reduct. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_STAXY

Accession

Primary (citable) accession number: Q01257

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)