Reviewed, UniProtKB/Swiss-Prot Q01237 (HMDH_MOUSE)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:

Hmgcr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane proteinBy similarity. Peroxisome membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane Peroxisome
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from direct assay. Source: MGI coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro embryonic development Inferred from mutant phenotype. Source: MGI isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro negative regulation of MAP kinase activity Inferred from genetic interaction. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW visual learning Inferred from genetic interaction. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from direct assay. Source: MGI peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114421
Regions
	Transmembrane	10 – 39	30	Potential
	Transmembrane	57 – 78	22	Potential
	Transmembrane	90 – 114	25	Potential
	Transmembrane	124 – 149	26	Potential
	Transmembrane	160 – 187	28	Potential
	Transmembrane	192 – 220	29	Potential
	Transmembrane	315 – 339	25	Potential
	Region	340 – 449	110	Linker
	Region	450 – 887	438	Catalytic
Sites
	Active site	558	1	Charge relay system By similarity
	Active site	690	1	Charge relay system By similarity
	Active site	766	1	Charge relay system By similarity
	Active site	865	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
	Glycosylation	517	1	N-linked (GlcNAc...) Potential
	Glycosylation	869	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01237-1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 30F3B40D0BEE37A6	FASTA	887	97,022
	10 20 30 40 50 60 MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC PKFEEDVLSS 70 80 90 100 110 120 DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 130 140 150 160 170 180 LNEALPFFLL LIDLSRASAL AKFALSSNSQ DEVRENIARG MAILGPTFTL DALVECLVIG 190 200 210 220 230 240 VGTMSGVRQL EIMCCFGCMS VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR 250 260 270 280 290 300 VLEEEENKPN PVTQRVKIIM SLGLVLVHAH SRWIADPSPQ NSTAEQAKVS LGLDEDVSKR 310 320 330 340 350 360 IEPSVSLWQF YLSKMISMDI EQVITLSLAF LLAVKYIFFE QAETESTLSL KNPITSPVVT 370 380 390 400 410 420 SKKAQDNCCR REPLLVRRNQ KLSSVEEDPG ANQERKVEVI KPLVVEAETT SRATFVLGAS 430 440 450 460 470 480 VASPPSALGT QEPGIELPIE PRPNEECLQI LENAEKGAKF LSDAEIIQLV NAKHIPAYKL 490 500 510 520 530 540 ETLMETHERG VSIRRQLLST KLAEPSSLQY LPYRDYNYSL VMGACCENVI GYMPIPVGVA 550 560 570 580 590 600 GPLCLDGKEY QVPMATTEGC LVASTNRGCR AISLGGGASS RVLADGMTRG PVVRLPRACD 610 620 630 640 650 660 SAEVKTWLET PEGFAVIKEA FDSTSRFARL QKLHVTMAGR NLYIRFQSRT GDAMGMNMIS 670 680 690 700 710 720 KGTEKALLKL QEFFPDMQIL AVSGNYCTDK KPAAINWIEG RGKTVVCEAV IPAKVVREVL 730 740 750 760 770 780 KTTTEAMVDV NINKNLVGSA MAGSIGGYNA HAANIVTAIY IACGQDAAQN VGSSNCITLM 790 800 810 820 830 840 EASGPTNEDL YISCTMPSIE IGTVGGGTNL LPQQACLQML GVQGACKDNP GENARQLARI 850 860 870 880 VCGTVMAGEL SLMAALAAGH LVRSHMVHNR SKINLQDLQG TCTKKAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Glucocorticoid-regulated gene expression in the immune system. Analysis of glucocorticoid-regulated transcripts from the mouse macrophage-like cell line P388D1." Helmberg A., Faessler R., Geley S., Joehrer K., Kroemer G., Boeck G., Kofler R. J. Immunol. 145:4332-4337(1990) [PubMed: 2258621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 664-887.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC085083 mRNA. Translation: AAH85083.1. M62766 mRNA. Translation: AAA37819.1.
PIR	A43533.
UniGene	Mm.316652
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
SMR	Q01237. Positions 461-869.
ModBase	Search...
PTM databases
PhosphoSite	Q01237.
Genome annotation databases
Ensembl	ENSMUSG00000021670. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:96159. Hmgcr.
Phylogenomic databases
HOGENOM	Q01237.
HOVERGEN	Q01237.
Gene expression databases
ArrayExpress	Q01237.
CleanEx	MM_HMGCR.
GermOnline	ENSMUSG00000021670. Mus musculus.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR004816. HMG_CoA_Rdtase_I_metazoan. IPR000731. SSD_5TM. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00920. 2A060605. 1 hit. TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

HMDH_MOUSE

Accession

Primary (citable) accession number: Q01237
Secondary accession number(s): Q5U4I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)