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Q01234

- NFNB_ENTCL

UniProt

Q01234 - NFNB_ENTCL

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Protein

Oxygen-insensitive NAD(P)H nitroreductase

Gene
nfnB, nfsI
Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred.

Cofactori

FMN.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411NAD or NADP; via amide nitrogen Inferred
Binding sitei71 – 711FMN

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145FMN
Nucleotide bindingi153 – 1586NAD or NADP By similarity
Nucleotide bindingi165 – 1662FMN
Nucleotide bindingi205 – 2073FMN

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-insensitive NAD(P)H nitroreductase (EC:1.-.-.-)
Short name:
NR
Gene namesi
Name:nfnB
Synonyms:nfsI
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Oxygen-insensitive NAD(P)H nitroreductasePRO_0000072712Add
BLAST

Proteomic databases

PRIDEiQ01234.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97
Helixi24 – 3613
Helixi40 – 423
Beta strandi46 – 516
Helixi54 – 607
Helixi61 – 633
Helixi66 – 716
Helixi72 – 776
Beta strandi78 – 8912
Helixi92 – 10413
Helixi111 – 12919
Turni130 – 1323
Helixi135 – 15622
Helixi169 – 1757
Helixi178 – 1814
Beta strandi183 – 19210
Helixi199 – 2013
Helixi210 – 2134
Beta strandi214 – 2174

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KQBX-ray1.80A/B/C/D1-217[»]
1KQCX-ray1.80A/B/C/D1-217[»]
1KQDX-ray1.90A/B/C/D1-217[»]
1NECX-ray1.95A/B/C/D2-217[»]
ProteinModelPortaliQ01234.
SMRiQ01234. Positions 2-217.

Miscellaneous databases

EvolutionaryTraceiQ01234.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitroreductase family.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01234-1 [UniParc]FASTAAdd to Basket

« Hide

MDIISVALKR HSTKAFDASK KLTAEEAEKI KTLLQYSPSS TNSQPWHFIV    50
ASTEEGKARV AKSAAGTYVF NERKMLDASH VVVFCAKTAM DDAWLERVVD 100
QEEADGRFNT PEAKAANHKG RTYFADMHRV DLKDDDQWMA KQVYLNVGNF 150
LLGVGAMGLD AVPIEGFDAA ILDEEFGLKE KGFTSLVVVP VGHHSVEDFN 200
ATLPKSRLPL STIVTEC 217
Length:217
Mass (Da):23,950
Last modified:July 1, 1993 - v1
Checksum:iC42AA3DB184D5D9B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63808 Genomic DNA. Translation: AAA62801.1.
PIRiA38686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63808 Genomic DNA. Translation: AAA62801.1 .
PIRi A38686.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KQB X-ray 1.80 A/B/C/D 1-217 [» ]
1KQC X-ray 1.80 A/B/C/D 1-217 [» ]
1KQD X-ray 1.90 A/B/C/D 1-217 [» ]
1NEC X-ray 1.95 A/B/C/D 2-217 [» ]
ProteinModelPortali Q01234.
SMRi Q01234. Positions 2-217.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q01234.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q01234.

Family and domain databases

Gene3Di 3.40.109.10. 1 hit.
InterProi IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view ]
Pfami PF00881. Nitroreductase. 1 hit.
[Graphical view ]
SUPFAMi SSF55469. SSF55469. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae."
    Bryant C., Hubbard L., McElroy W.D.
    J. Biol. Chem. 266:4126-4130(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43560 / 96-3.
  2. "Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae."
    Bryant C., Deluca M.
    J. Biol. Chem. 266:4119-4125(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "Structures of nitroreductase in three states: effects of inhibitor binding and reduction."
    Haynes C.A., Koder R.L., Miller A.-F., Rodgers D.W.
    J. Biol. Chem. 277:11513-11520(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEXES WITH FMN; ACETATE AND BENZOIC ACID, SUBUNIT.

Entry informationi

Entry nameiNFNB_ENTCL
AccessioniPrimary (citable) accession number: Q01234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The nitroreductase might be involved in the quinone metabolism.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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