Q01234 (NFNB_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxygen-insensitive NAD(P)H nitroreductase
Short name=NR
EC=1.-.-.-

Gene names

Name:	nfnB
Synonyms:	nfsI

Organism

Enterobacter cloacae

Taxonomic identifier

550 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred.
Cofactor	FMN.
Subunit structure	Homodimer. Ref.3
Miscellaneous	The nitroreductase might be involved in the quinone metabolism.
Sequence similarities	Belongs to the nitroreductase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 217

217

Oxygen-insensitive NAD(P)H nitroreductase

PRO_0000072712

Regions

Nucleotide binding

10 – 14

5

FMN

Nucleotide binding

153 – 158

6

NAD or NADP By similarity

Nucleotide binding

165 – 166

2

FMN

Nucleotide binding

205 – 207

3

FMN

Sites

Binding site

41

1

NAD or NADP; via amide nitrogen Probable

Binding site

71

1

FMN

Secondary structure

1

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217

Helix Strand Turn

Details...

Helix

3 – 9

7

Helix

24 – 36

13

Helix

40 – 42

3

Beta strand

46 – 51

6

Helix

54 – 60

7

Helix

61 – 63

3

Helix

66 – 71

6

Helix

72 – 77

6

Beta strand

78 – 89

12

Helix

92 – 104

13

Helix

111 – 129

19

Turn

130 – 132

3

Helix

135 – 156

22

Helix

169 – 175

7

Helix

178 – 181

4

Beta strand

183 – 192

10

Helix

199 – 201

3

Helix

210 – 213

4

Beta strand

214 – 217

4

Sequences

	Sequence		Length	Mass (Da)
	Q01234 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: C42AA3DB184D5D9B	FASTA	217	23,950
	10 20 30 40 50 60 MDIISVALKR HSTKAFDASK KLTAEEAEKI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV 70 80 90 100 110 120 AKSAAGTYVF NERKMLDASH VVVFCAKTAM DDAWLERVVD QEEADGRFNT PEAKAANHKG 130 140 150 160 170 180 RTYFADMHRV DLKDDDQWMA KQVYLNVGNF LLGVGAMGLD AVPIEGFDAA ILDEEFGLKE 190 200 210 KGFTSLVVVP VGHHSVEDFN ATLPKSRLPL STIVTEC

References

[1]	"Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae." Bryant C., Hubbard L., McElroy W.D. J. Biol. Chem. 266:4126-4130(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43560 / 96-3.
[2]	"Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae." Bryant C., Deluca M. J. Biol. Chem. 266:4119-4125(1991) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Structures of nitroreductase in three states: effects of inhibitor binding and reduction." Haynes C.A., Koder R.L., Miller A.-F., Rodgers D.W. J. Biol. Chem. 277:11513-11520(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEXES WITH FMN; ACETATE AND BENZOIC ACID, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63808 Genomic DNA. Translation: AAA62801.1.

PIR

A38686.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KQB	X-ray	1.80	A/B/C/D	1-217	[»]
1KQC	X-ray	1.80	A/B/C/D	1-217	[»]
1KQD	X-ray	1.90	A/B/C/D	1-217	[»]
1NEC	X-ray	1.95	A/B/C/D	2-217	[»]

ProteinModelPortal

Q01234.

SMR

Q01234. Positions 2-217.

ModBase

Search...

Proteomic databases

PRIDE

Q01234.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.40.109.10. 1 hit.

InterPro

IPR000415. Nitroreductase-like.
[Graphical view]

Pfam

PF00881. Nitroreductase. 1 hit.
[Graphical view]

SUPFAM

SSF55469. Nitroreductase. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q01234.

Entry information

Entry name

NFNB_ENTCL

Accession

Primary (citable) accession number: Q01234

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program