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Q01234

- NFNB_ENTCL

UniProt

Q01234 - NFNB_ENTCL

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Protein

Oxygen-insensitive NAD(P)H nitroreductase

Gene

nfnB

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred.

Cofactori

FMN.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411NAD or NADP; via amide nitrogenCurated
Binding sitei71 – 711FMN

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145FMN
Nucleotide bindingi153 – 1586NAD or NADPBy similarity
Nucleotide bindingi165 – 1662FMN
Nucleotide bindingi205 – 2073FMN

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-insensitive NAD(P)H nitroreductase (EC:1.-.-.-)
Short name:
NR
Gene namesi
Name:nfnB
Synonyms:nfsI
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Oxygen-insensitive NAD(P)H nitroreductasePRO_0000072712Add
BLAST

Proteomic databases

PRIDEiQ01234.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97
Helixi24 – 3613
Helixi40 – 423
Beta strandi46 – 516
Helixi54 – 607
Helixi61 – 633
Helixi66 – 716
Helixi72 – 776
Beta strandi78 – 8912
Helixi92 – 10413
Helixi111 – 12919
Turni130 – 1323
Helixi135 – 15622
Helixi169 – 1757
Helixi178 – 1814
Beta strandi183 – 19210
Helixi199 – 2013
Helixi210 – 2134
Beta strandi214 – 2174

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KQBX-ray1.80A/B/C/D1-217[»]
1KQCX-ray1.80A/B/C/D1-217[»]
1KQDX-ray1.90A/B/C/D1-217[»]
1NECX-ray1.95A/B/C/D2-217[»]
ProteinModelPortaliQ01234.
SMRiQ01234. Positions 2-217.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01234.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitroreductase family.Curated

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01234-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDIISVALKR HSTKAFDASK KLTAEEAEKI KTLLQYSPSS TNSQPWHFIV
60 70 80 90 100
ASTEEGKARV AKSAAGTYVF NERKMLDASH VVVFCAKTAM DDAWLERVVD
110 120 130 140 150
QEEADGRFNT PEAKAANHKG RTYFADMHRV DLKDDDQWMA KQVYLNVGNF
160 170 180 190 200
LLGVGAMGLD AVPIEGFDAA ILDEEFGLKE KGFTSLVVVP VGHHSVEDFN
210
ATLPKSRLPL STIVTEC
Length:217
Mass (Da):23,950
Last modified:July 1, 1993 - v1
Checksum:iC42AA3DB184D5D9B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63808 Genomic DNA. Translation: AAA62801.1.
PIRiA38686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63808 Genomic DNA. Translation: AAA62801.1 .
PIRi A38686.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KQB X-ray 1.80 A/B/C/D 1-217 [» ]
1KQC X-ray 1.80 A/B/C/D 1-217 [» ]
1KQD X-ray 1.90 A/B/C/D 1-217 [» ]
1NEC X-ray 1.95 A/B/C/D 2-217 [» ]
ProteinModelPortali Q01234.
SMRi Q01234. Positions 2-217.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q01234.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q01234.

Family and domain databases

Gene3Di 3.40.109.10. 1 hit.
InterProi IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view ]
Pfami PF00881. Nitroreductase. 1 hit.
[Graphical view ]
SUPFAMi SSF55469. SSF55469. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae."
    Bryant C., Hubbard L., McElroy W.D.
    J. Biol. Chem. 266:4126-4130(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43560 / 96-3.
  2. "Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae."
    Bryant C., Deluca M.
    J. Biol. Chem. 266:4119-4125(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "Structures of nitroreductase in three states: effects of inhibitor binding and reduction."
    Haynes C.A., Koder R.L., Miller A.-F., Rodgers D.W.
    J. Biol. Chem. 277:11513-11520(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEXES WITH FMN; ACETATE AND BENZOIC ACID, SUBUNIT.

Entry informationi

Entry nameiNFNB_ENTCL
AccessioniPrimary (citable) accession number: Q01234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The nitroreductase might be involved in the quinone metabolism.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3