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Q01234 (NFNB_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oxygen-insensitive NAD(P)H nitroreductase

Short name=NR
EC=1.-.-.-
Gene names
Name:nfnB
Synonyms:nfsI
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred.

Cofactor

FMN.

Subunit structure

Homodimer. Ref.3

Miscellaneous

The nitroreductase might be involved in the quinone metabolism.

Sequence similarities

Belongs to the nitroreductase family.

Ontologies

Keywords
   LigandFlavoprotein
FMN
NAD
NADP
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Molecular_functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217Oxygen-insensitive NAD(P)H nitroreductase
PRO_0000072712

Regions

Nucleotide binding10 – 145FMN
Nucleotide binding153 – 1586NAD or NADP By similarity
Nucleotide binding165 – 1662FMN
Nucleotide binding205 – 2073FMN

Sites

Binding site411NAD or NADP; via amide nitrogen Probable
Binding site711FMN

Secondary structure

................................. 217
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q01234 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C42AA3DB184D5D9B

FASTA21723,950
        10         20         30         40         50         60 
MDIISVALKR HSTKAFDASK KLTAEEAEKI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV 

        70         80         90        100        110        120 
AKSAAGTYVF NERKMLDASH VVVFCAKTAM DDAWLERVVD QEEADGRFNT PEAKAANHKG 

       130        140        150        160        170        180 
RTYFADMHRV DLKDDDQWMA KQVYLNVGNF LLGVGAMGLD AVPIEGFDAA ILDEEFGLKE 

       190        200        210 
KGFTSLVVVP VGHHSVEDFN ATLPKSRLPL STIVTEC 

« Hide

References

[1]"Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae."
Bryant C., Hubbard L., McElroy W.D.
J. Biol. Chem. 266:4126-4130(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43560 / 96-3.
[2]"Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae."
Bryant C., Deluca M.
J. Biol. Chem. 266:4119-4125(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[3]"Structures of nitroreductase in three states: effects of inhibitor binding and reduction."
Haynes C.A., Koder R.L., Miller A.-F., Rodgers D.W.
J. Biol. Chem. 277:11513-11520(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEXES WITH FMN; ACETATE AND BENZOIC ACID, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63808 Genomic DNA. Translation: AAA62801.1.
PIRA38686.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KQBX-ray1.80A/B/C/D1-217[»]
1KQCX-ray1.80A/B/C/D1-217[»]
1KQDX-ray1.90A/B/C/D1-217[»]
1NECX-ray1.95A/B/C/D2-217[»]
ProteinModelPortalQ01234.
SMRQ01234. Positions 2-217.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ01234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.109.10. 1 hit.
InterProIPR000415. Nitroreductase-like.
[Graphical view]
PfamPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMSSF55469. SSF55469. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ01234.

Entry information

Entry nameNFNB_ENTCL
AccessionPrimary (citable) accession number: Q01234
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 16, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references