Q01231 (CXA5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction alpha-5 protein

Alternative name(s):
Connexin-40
Short name=Cx40

Gene names

Name:	Gja5
Synonyms:	Cxn-40

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Kidney, heart and skin, but most abundant in lung.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	AV node cell to bundle of His cell communication Inferred from mutant phenotype PubMed 10515563. Source: MGI angiogenesis Inferred from electronic annotation. Source: Compara artery morphogenesis Inferred from mutant phenotype PubMed 20530546. Source: BHF-UCL atrial septum morphogenesis Inferred from mutant phenotype PubMed 10969038. Source: MGI atrial ventricular junction remodeling Inferred from mutant phenotype PubMed 10969038. Source: MGI cardiac conduction system development Inferred from mutant phenotype PubMed 18599871PubMed 22203979. Source: MGI cell communication by electrical coupling involved in cardiac conduction Inferred from mutant phenotype PubMed 11046183. Source: MGI embryonic heart tube development Inferred from mutant phenotype PubMed 14732399PubMed 22203979. Source: MGI embryonic limb morphogenesis Inferred from mutant phenotype PubMed 15923624. Source: MGI foramen ovale closure Inferred from mutant phenotype PubMed 10969038. Source: MGI regulation of SA node cell action potential Inferred from mutant phenotype PubMed 11046183. Source: MGI regulation of atrial cardiac muscle cell membrane depolarization Inferred from mutant phenotype PubMed 10515563PubMed 10969038PubMed 9501070. Source: MGI regulation of heart rate by cardiac conduction Inferred from mutant phenotype PubMed 11046183. Source: MGI regulation of membrane depolarization involved in regulation of cardiac muscle cell action potential Inferred from mutant phenotype PubMed 9501070. Source: MGI regulation of renin secretion into blood stream Inferred from mutant phenotype PubMed 17255527. Source: MGI regulation of vasodilation Inferred from mutant phenotype PubMed 10747000. Source: MGI regulation of ventricular cardiac muscle cell action potential Inferred from mutant phenotype PubMed 9501070. Source: MGI regulation of ventricular cardiac muscle cell membrane depolarization Inferred from mutant phenotype PubMed 10515563PubMed 9501070. Source: MGI regulation of ventricular cardiac muscle cell membrane repolarization Inferred from mutant phenotype PubMed 10969038. Source: MGI renal system process involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 17255527. Source: MGI septum primum development Inferred from mutant phenotype PubMed 10969038. Source: MGI skeletal system development Inferred from mutant phenotype PubMed 15923624. Source: MGI ventricular septum morphogenesis Inferred from mutant phenotype PubMed 10969038. Source: MGI
Cellular_component	connexon complex Inferred from electronic annotation. Source: Compara gap junction Inferred from direct assay PubMed 9501070. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	gap junction channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Gap junction alpha-5 protein		PRO_0000057820
Regions
	Topological domain	1 – 19	19	Cytoplasmic Potential
	Transmembrane	20 – 40	21	Helical; Potential
	Topological domain	41 – 76	36	Extracellular Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Topological domain	98 – 164	67	Cytoplasmic Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Topological domain	186 – 205	20	Extracellular Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Topological domain	227 – 358	132	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01231 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C5418F657142896A	FASTA	358	40,413
	10 20 30 40 50 60 MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG 70 80 90 100 110 120 CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRD AEKAKEAHRT 130 140 150 160 170 180 GAYEYPVAEK AELSCWKEVD GKIVLQGTLL NTYVCTILIR TTMEVAFIVG QYLLYGIFLD 190 200 210 220 230 240 TLHVCRRSPC PHPVNCYVSR PTEKNVFIVF MMAVAGLSLF LSLAELYHLG WKKIRQRFGK 250 260 270 280 290 300 SRQGVDKHQL PGPPTSLVQS LTPPPDFNQC LKNSSGEKFF SDFSNNMGSR KNPDALATGE 310 320 330 340 350 VPNQEQIPGE GFIHMHYSQK PEYASGASAG HRLPQGYHSD KRRLSKASSK ARSDDLSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung." Hennemann J., Suchyna T., Lichtenberg-Frate H., Jungbluth S., Dahl E., Schwarz J., Nicholson B.J., Willecke K. J. Cell Biol. 117:1299-1310(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61675 Genomic DNA. Translation: CAA43850.1. BC053054 mRNA. Translation: AAH53054.1.
IPI	IPI00875783.
PIR	S23111.
RefSeq	NP_001258557.1. NM_001271628.1. NP_032147.1. NM_008121.3.
UniGene	Mm.281816.
3D structure databases
ProteinModelPortal	Q01231.
SMR	Q01231. Positions 3-229, 251-358.
ModBase	Search...
PTM databases
PhosphoSite	Q01231.
Proteomic databases
PRIDE	Q01231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000072600; ENSMUSP00000088264; ENSMUSG00000057123.
GeneID	14613.
KEGG	mmu:14613.
UCSC	uc008qop.1. mouse.
Organism-specific databases
CTD	2702.
MGI	MGI:95716. Gja5.
Phylogenomic databases
eggNOG	NOG45265.
GeneTree	ENSGT00700000104145.
HOGENOM	HOG000231127.
HOVERGEN	HBG009576.
KO	K07614.
OMA	DWSFLGE.
OrthoDB	EOG4MSCZF.
Gene expression databases
ArrayExpress	Q01231.
Bgee	Q01231.
CleanEx	MM_GJA5.
Genevestigator	Q01231.
GermOnline	ENSMUSG00000057123. Mus musculus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002264. Connexin40. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit. PTHR11984:SF13. PTHR11984:SF13. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01135. CONNEXINA5.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286420.
SOURCE	Search...

Entry information

Entry name

CXA5_MOUSE

Accession

Primary (citable) accession number: Q01231

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program