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Q01213 (DTDH_MUCMU) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-dihydromethyl-trisporate dehydrogenase
Short name=4-dihydromethyl-TA dehydrogenase
EC=1.1.1.-
Gene names
Name:tdh
OrganismMucor mucedo (Common pinmould)
Taxonomic identifier29922 [NCBI]
Taxonomic lineageEukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NADP-dependent oxidation of (+) mating-type specific precursor 4-dihydromethyl-trisporate to methyl-trisporate. Ref.3

Pathway

Pheromone biosynthesis; trisporate biosynthesis.

Sequence similarities

Belongs to the aldo/keto reductase family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processtrisporic acid biosynthetic process

Non-traceable author statement Ref.1. Source: UniProtKB

   Molecular functionoxidoreductase activity, acting on hydrogen as donor, NAD or NADP as acceptor

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3213214-dihydromethyl-trisporate dehydrogenase
PRO_0000124677

Sites

Active site511Proton donor By similarity UniProtKB P06632
Binding site1131Substrate By similarity
Site801Lowers pKa of active site Tyr By similarity

Experimental info

Sequence conflict401T → M AA sequence Ref.1
Sequence conflict491C → E AA sequence Ref.1
Sequence conflict2551G → N AA sequence Ref.1
Sequence conflict2711P → I AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q01213 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AA64AED8DC95EC37

FASTA32136,484
        10         20         30         40         50         60 
MSTDYLTLNR TGDKMPIRGF GCWKIDTKDC EETVYQAIKT GYRLFDGACD YGNEVEVGRG 

        70         80         90        100        110        120 
INKAINEGLV KREDLFIVTK LWNTFHSKKH VRALFDRQLK DTGLEYFDLY LIHFPVPLQY 

       130        140        150        160        170        180 
VDPATVYPPG WYVGDAKSLQ FEQSPIHECW AELEKIVDAG LARNIGVANF NCQAILDLLT 

       190        200        210        220        230        240 
YARIKPAVLQ IELHPYLPQE RLVKWVKEQG IQITAYSSFG PTSYVDLTES GKTYTSLLEH 

       250        260        270        280        290        300 
ASVKSVADKH NVSTGQVLLR WALDREFAVI PKSVNAGRMK ANLEILDIKL DAEDNKTLDS 

       310        320 
LKTNQRFNDP MTYGFGLPLF D

« Hide

References

[1]"4-dihydromethyltrisporate dehydrogenase from Mucor mucedo, an enzyme of the sexual hormone pathway: purification, and cloning of the corresponding gene."
Czempinski K., Kruft V., Woestemeyer J., Burmester A.
Microbiology 142:2647-2654(1996) [PubMed: 8828234] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-52; 72-88; 233-244 AND 255-272.
Strain: CBS 109.16.
Tissue: Mycelium.
[2]"4-dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway is posttranscriptionally regulated in Mucor mucedo."
Petzold A., Burmester A., Wolschendorf F., Schultze K., Woestemeyer J., Schimek C.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CBS 144.24.
[3]"Localization and partial characterization of a sex-specific enzyme in homothallic and heterothallic mucorales."
Werkman B.A.
Arch. Microbiol. 109:209-213(1976)
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z73640 Genomic DNA. Translation: CAA98021.1.
AJ831376 Genomic DNA. Translation: CAH40839.1.

3D structure databases

ProteinModelPortalQ01213.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. False negative.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDTDH_MUCMU
AccessionPrimary (citable) accession number: Q01213
Secondary accession number(s): Q659V0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: November 1, 1996
Last modified: September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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