Reviewed, UniProtKB/Swiss-Prot Q01213 (DTDH_MUCMU)

Names and origin

Protein names

Recommended name:
    4-dihydromethyl-trisporate dehydrogenase
      Short name=4-dihydromethyl-TA dehydrogenase
    EC=1.1.1.-

Gene names

Name:

tdh

Organism

Mucor mucedo

Taxonomic identifier

29922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADP-dependent oxidation of (+) mating-type specific precursor 4-dihydromethyl-trisporate to methyl-trisporate. Ref.3
Pathway	Pheromone biosynthesis; trisporic acid biosynthesis.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW trisporic acid biosynthetic process Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	oxidoreductase activity, acting on hydrogen as donor, NAD or NADP as acceptor Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	4-dihydromethyl-trisporate dehydrogenase		PRO_0000124677
Sites
	Active site	51	1	Proton donor By similarity UniProtKB P06632
	Binding site	113	1	Substrate By similarity
	Site	80	1	Lowers pKa of active site Tyr By similarity
Experimental info
	Sequence conflict	40	1	T → M AA sequence Ref.1
	Sequence conflict	49	1	C → E AA sequence Ref.1
	Sequence conflict	255	1	G → N AA sequence Ref.1
	Sequence conflict	271	1	P → I AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01213-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AA64AED8DC95EC37	FASTA	321	36,484
	10 20 30 40 50 60 MSTDYLTLNR TGDKMPIRGF GCWKIDTKDC EETVYQAIKT GYRLFDGACD YGNEVEVGRG 70 80 90 100 110 120 INKAINEGLV KREDLFIVTK LWNTFHSKKH VRALFDRQLK DTGLEYFDLY LIHFPVPLQY 130 140 150 160 170 180 VDPATVYPPG WYVGDAKSLQ FEQSPIHECW AELEKIVDAG LARNIGVANF NCQAILDLLT 190 200 210 220 230 240 YARIKPAVLQ IELHPYLPQE RLVKWVKEQG IQITAYSSFG PTSYVDLTES GKTYTSLLEH 250 260 270 280 290 300 ASVKSVADKH NVSTGQVLLR WALDREFAVI PKSVNAGRMK ANLEILDIKL DAEDNKTLDS 310 320 LKTNQRFNDP MTYGFGLPLF D

References

[1]	"4-dihydromethyltrisporate dehydrogenase from Mucor mucedo, an enzyme of the sexual hormone pathway: purification, and cloning of the corresponding gene." Czempinski K., Kruft V., Woestemeyer J., Burmester A. Microbiology 142:2647-2654(1996) [PubMed: 8828234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-52; 72-88; 233-244 AND 255-272. Strain: CBS 109.16. Tissue: Mycelium.
[2]	"4-dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway is posttranscriptionally regulated in Mucor mucedo." Petzold A., Burmester A., Wolschendorf F., Schultze K., Woestemeyer J., Schimek C. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CBS 144.24.
[3]	"Localization and partial characterization of a sex-specific enzyme in homothallic and heterothallic mucorales." Werkman B.A. Arch. Microbiol. 109:209-213(1976) Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73640 Genomic DNA. Translation: CAA98021.1. AJ831376 Genomic DNA. Translation: CAH40839.1.
3D structure databases
HSSP	HSSP built from PDB template 1K8C based on UniProtKB O74237.
ModBase	Search...
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DTDH_MUCMU

Accession

Primary (citable) accession number: Q01213
Secondary accession number(s): Q659V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)