Q01198 (LIGD_PSEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C alpha-dehydrogenase
EC=1.-.-.-

Gene names

Name:

ligD

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis)

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the C alpha dehydrogenation of arylglycerol-beta-aryl ether (C alpha alcohol type) (compound IV).
Pathway	Secondary metabolite metabolism; lignin degradation.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lignin degradation
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	C alpha-dehydrogenase		PRO_0000054717
Regions
	Nucleotide binding	10 – 34	25	NAD or NADP By similarity
Sites
	Active site	157	1	Proton acceptor By similarity
	Binding site	144	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01198 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 6071D1451A928900	FASTA	305	32,344
	10 20 30 40 50 60 MKDFQDQVAF ITGGASGAGF GQAKVFGQAG AKIVVADVRA EAVEKAVAEL EGLGITAHGI 70 80 90 100 110 120 VLDIMDREAY ARAADEVEAV FGQAPTLLSN TAGVNSFGPI EKTTYDDFDW IIGVNLNGVI 130 140 150 160 170 180 NGMVTFVPRM IASGRPGHIV TVSSLGGFMG SALAGPYSAA KAASINLMEG YRQGLEKYGI 190 200 210 220 230 240 GVSVCTPANI KSNIAEASRL RPAKYGTSGY VENEESIASL HSIHQHGLEP EKLAEAIKKG 250 260 270 280 290 300 VEDNALYIIP YPEVREGLEK HFQAIIDSVA PMESDPEGAR QRVEALMAWG RDRTRVFAEG DKKGA

References

[1]	"Cloning and sequencing of the gene for a Pseudomonas paucimobilis enzyme that cleaves beta-aryl ether." Masai E., Katayama Y., Kawai S., Nishikawa S., Yamasaki M., Morohoshi N. J. Bacteriol. 173:7950-7955(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SYK-6.
[2]	"A bacterial enzyme degrading the model lignin compound beta-etherase is a member of the glutathione-S-transferase superfamily." Masai E., Katayama Y., Kubota S., Kawai S., Yamasaki M., Morohoshi N. FEBS Lett. 323:135-140(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of the C alpha-dehydrogenase gene involved in the cleavage of beta-aryl ether by Pseudomonas paucimobilis." Masai E., Kubota S., Katayama Y., Kawai S., Yamasaki M., Morohoshi N. Biosci. Biotechnol. Biochem. 57:1655-1659(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	S67975 Genomic DNA. Translation: AAC60455.1. D11473 Genomic DNA. Translation: BAA02030.1. D11341 Genomic DNA. Translation: BAA01953.1.
PIR	S35991.
3D structure databases
ProteinModelPortal	Q01198.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15126.
UniPathway	UPA00892.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIGD_PSEPA

Accession

Primary (citable) accession number: Q01198

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program