Reviewed, UniProtKB/Swiss-Prot Q01170 (NIA_CHLVU)

Names and origin

Protein names	Recommended name:     Nitrate reductase [NADH]       Short name=NR     EC=1.7.1.1
Organism	Chlorella vulgaris (Green alga)
Taxonomic identifier	3077 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Chlorophyta › Trebouxiophyceae › Chlorellales › Chlorellaceae › Chlorella

Protein attributes

Sequence length	318 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD per subunit. Binds 1 heme group per subunit. Binds 1 molybdenum-pterin group per subunit.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›318	›318	Nitrate reductase [NADH]		PRO_0000166053
Regions
	Domain	216 – 291	76	Cytochrome b5 heme-binding
Sites
	Metal binding	251	1	Iron (heme axial ligand) By similarity
	Metal binding	274	1	Iron (heme axial ligand) By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	318	1

Sequences

	Sequence		Length	Mass (Da)
	Q01170-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E60D82FE1E98292A	FASTA	318	34,830
	10 20 30 40 50 60 RPPVTEVESQ NYYHFHDNRV LPSHVDEALA NSEGWWYKPD FIINDLNVQS AIGYPAHEEV 70 80 90 100 110 120 VPLVAGTYAV RGYARGHGNK IIRCEVSLDD GKSWRLGSVT HEGQPTEYGK HWGWVWWSLE 130 140 150 160 170 180 VPIAELLTTP EIICRAWDSS MNTQPNTFTW NVMGMMNNCC YRVKIHPRQT TDGRFALQFE 190 200 210 220 230 240 HPTIAGPTVG GWMNRAEDVA AAAAVTVAPP PAPAGAKSFT MAEVETHTTM ESAWFVVDGK 250 260 270 280 290 300 VYDATPFLKD HPGGADSILL VAGIDATDEF NAIHSLKAKK QLLEYYIGEL AEEGQEAAAS 310 DRATPGPAAA IGTAVPVA

References

[1]	"Expression of a cDNA clone encoding the haem-binding domain of Chlorella nitrate reductase." Cannons A.C., Iida N., Solomonson L.P. Biochem. J. 278:203-209(1991) [PubMed: 1883330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56771 mRNA. Translation: CAA40090.1.
PIR	S17197.
3D structure databases
HSSP	HSSP built from PDB template 1B5M based on UniProtKB P04166.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.1. 2068.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF03404. Mo-co_dimer. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS00559. MOLYBDOPTERIN_EUK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_CHLVU

Accession

Primary (citable) accession number: Q01170

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)