Reviewed, UniProtKB/Swiss-Prot Q01158 (LUCI_LUCLA)

Names and origin

Protein names	Recommended name:     Luciferin 4-monooxygenase       Short name=Luciferase     EC=1.13.12.7
Organism	Luciola lateralis (Firefly)
Taxonomic identifier	7052 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Elateriformia › Elateroidea › Lampyridae › Luciolinae › Luciola

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces green light.
Catalytic activity	Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.
Cofactor	Magnesium.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Luminescence
Cellular component	Peroxisome
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Luciferin 4-monooxygenase		PRO_0000193144
Regions
	Motif	546 – 548	3	Microbody targeting signal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01158-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: AC62F9320BB6D4A6	FASTA	548	60,125
	10 20 30 40 50 60 MENMENDENI VYGPEPFYPI EEGSAGAQLR KYMDRYAKLG AIAFTNALTG VDYTYAEYLE 70 80 90 100 110 120 KSCCLGEALK NYGLVVDGRI ALCSENCEEF FIPVLAGLFI GVGVAPTNEI YTLRELVHSL 130 140 150 160 170 180 GISKPTIVFS SKKGLDKVIT VQKTVTAIKT IVILDSKVDY RGYQSMDNFI KKNTPQGFKG 190 200 210 220 230 240 SSFKTVEVNR KEQVALIMNS SGSTGLPKGV QLTHENAVTR FSHARDPIYG NQVSPGTAIL 250 260 270 280 290 300 TVVPFHHGFG MFTTLGYLTC GFRIVMLTKF DEETFLKTLQ DYKCSSVILV PTLFAILNRS 310 320 330 340 350 360 ELLDKYDLSN LVEIASGGAP LSKEIGEAVA RRFNLPGVRQ GYGLTETTSA IIITPEGDDK 370 380 390 400 410 420 PGASGKVVPL FKAKVIDLDT KKTLGPNRRG EVCVKGPMLM KGYVDNPEAT REIIDEEGWL 430 440 450 460 470 480 HTGDIGYYDE EKHFFIVDRL KSLIKYKGYQ VPPAELESVL LQHPNIFDAG VAGVPDPIAG 490 500 510 520 530 540 ELPGAVVVLE KGKSMTEKEV MDYVASQVSN AKRLRGGVRF VDEVPKGLTG KIDGKAIREI LKKPVAKM

References

[1]	"Molecular cloning and expression in Escherichia coli of a cDNA clone encoding luciferase of a firefly, Luciola lateralis." Tatsumi H., Kajiyama N., Nakano E. Biochim. Biophys. Acta 1131:161-165(1992) [PubMed: 1610896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66919 mRNA. Translation: CAA47358.1.
PIR	S23437.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
SMR	Q01158. Positions 7-545.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.12.7. 262202.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUCI_LUCLA

Accession

Primary (citable) accession number: Q01158

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)