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Protein

Choline kinase alpha

Gene

Chka

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathway: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
Proteins known to be involved in this subpathway in this organism are:
  1. Choline kinase alpha (Chka)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Pathway: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline kinase alpha (Chka), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei142 – 1421ATPBy similarity
Binding sitei304 – 3041ATPBy similarity
Binding sitei326 – 3261ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi113 – 1197ATPBy similarity
Nucleotide bindingi203 – 2097ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: RGD
  • choline binding Source: RGD
  • choline kinase activity Source: UniProtKB
  • cholinesterase activity Source: Ensembl
  • drug binding Source: UniProtKB
  • ethanolamine kinase activity Source: UniProtKB

GO - Biological processi

  • CDP-choline pathway Source: GOC
  • choline metabolic process Source: RGD
  • phosphatidylcholine biosynthetic process Source: UniProtKB
  • phosphatidylethanolamine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_284894. Synthesis of PE.
REACT_351400. Synthesis of PC.
SABIO-RKQ01134.
UniPathwayiUPA00558; UER00741.
UPA00753; UER00737.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline kinase alpha (EC:2.7.1.32)
Short name:
CK
Alternative name(s):
CHETK-alpha
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
Gene namesi
Name:Chka
Synonyms:Chk, Ckr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi61944. Chka.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Choline kinase alphaPRO_0000206221Add
BLAST

Proteomic databases

PaxDbiQ01134.
PRIDEiQ01134.

Expressioni

Tissue specificityi

Testis, brain, lung, kidney and liver.

Gene expression databases

ExpressionAtlasiQ01134. baseline and differential.
GenevisibleiQ01134. RN.

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKB.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023020.

Structurei

3D structure databases

ProteinModelPortaliQ01134.
SMRiQ01134. Positions 83-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 1173Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 8129Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiQ01134.
KOiK14156.
OMAiRAYLWCK.
OrthoDBiEOG72VH68.
PhylomeDBiQ01134.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01134-1) [UniParc]FASTAAdd to basket

Also known as: R2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTKFCTGGE AEPSPLGLLL SCGGSAAPTP GVGQQRDAAG ELESKQLGGR
60 70 80 90 100
SQPLALPPPP PPPLPLPPPP SPPLADEQPE PRTRRRAYLW CKEFLPGAWR
110 120 130 140 150
GLREDQFHIS VIRGGLSNML FQCSLPDSIA SVGDEPRKVL LRLYGAILKM
160 170 180 190 200
RSCNKEGSEQ AQNENEFQGA EAMVLESVMF AILAERSLGP KLYGIFPQGR
210 220 230 240 250
LEQFIPSRRL DTEELCLPDI SAEIAEKMAT FHGMKMPFNK EPKWLFGTME
260 270 280 290 300
KYLNQVLRLK FSREARVQQL HKFLSYNLPL ELENLRSLLQ YTRSPVVFCH
310 320 330 340 350
NDCQEGNILL LEGQENSEKQ KLMLIDFEYS SYNYRGFDIG NHFCEWMYDY
360 370 380 390 400
TYEKYPFFRA NIQKYPTRKQ QLHFISSYLT TFQNDFESLS SEEQSATKED
410 420 430 440 450
MLLEVNRFAL ASHFLWGLWS IVQAKISSIE FGYMEYAQAR FDAYFDQKRK

LGV
Length:453
Mass (Da):51,824
Last modified:May 30, 2000 - v2
Checksum:iDBF5643F321F0A2E
GO
Isoform 2 (identifier: Q01134-2) [UniParc]FASTAAdd to basket

Also known as: R1

The sequence of this isoform differs from the canonical sequence as follows:
     151-168: Missing.

Show »
Length:435
Mass (Da):49,744
Checksum:iA3EA4DD27652BE61
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei151 – 16818Missing in isoform 2. CuratedVSP_001067Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10261 mRNA. Translation: BAA01102.1.
D37884 Genomic DNA. Translation: BAA07126.1.
D37885 mRNA. Translation: BAA07127.1.
PIRiA42672.
JX0342.
RefSeqiNP_058823.1. NM_017127.1. [Q01134-2]
XP_006230770.1. XM_006230708.2. [Q01134-1]
UniGeneiRn.10985.

Genome annotation databases

EnsembliENSRNOT00000022824; ENSRNOP00000022824; ENSRNOG00000016791. [Q01134-2]
ENSRNOT00000023020; ENSRNOP00000023020; ENSRNOG00000016791. [Q01134-1]
GeneIDi29194.
KEGGirno:29194.
UCSCiRGD:61944. rat. [Q01134-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10261 mRNA. Translation: BAA01102.1.
D37884 Genomic DNA. Translation: BAA07126.1.
D37885 mRNA. Translation: BAA07127.1.
PIRiA42672.
JX0342.
RefSeqiNP_058823.1. NM_017127.1. [Q01134-2]
XP_006230770.1. XM_006230708.2. [Q01134-1]
UniGeneiRn.10985.

3D structure databases

ProteinModelPortaliQ01134.
SMRiQ01134. Positions 83-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023020.

Proteomic databases

PaxDbiQ01134.
PRIDEiQ01134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022824; ENSRNOP00000022824; ENSRNOG00000016791. [Q01134-2]
ENSRNOT00000023020; ENSRNOP00000023020; ENSRNOG00000016791. [Q01134-1]
GeneIDi29194.
KEGGirno:29194.
UCSCiRGD:61944. rat. [Q01134-1]

Organism-specific databases

CTDi1119.
RGDi61944. Chka.

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiQ01134.
KOiK14156.
OMAiRAYLWCK.
OrthoDBiEOG72VH68.
PhylomeDBiQ01134.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
UPA00753; UER00737.
ReactomeiREACT_284894. Synthesis of PE.
REACT_351400. Synthesis of PC.
SABIO-RKQ01134.

Miscellaneous databases

NextBioi608327.
PROiQ01134.

Gene expression databases

ExpressionAtlasiQ01134. baseline and differential.
GenevisibleiQ01134. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, characterization, and expression in Escherichia coli of a cDNA encoding mammalian choline kinase."
    Uchida T., Yamashita S.
    J. Biol. Chem. 267:10156-10162(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
    Strain: Wistar.
    Tissue: Liver.
  2. "Regulation of choline kinase R: analyses of alternatively spliced choline kinases and the promoter region."
    Uchida T.
    J. Biochem. 116:508-518(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley and Wistar.
    Tissue: Liver and Testis.

Entry informationi

Entry nameiCHKA_RAT
AccessioniPrimary (citable) accession number: Q01134
Secondary accession number(s): Q63114
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 30, 2000
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.