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Q01134 (CHKA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Choline kinase alpha
Short name=CK
EC=2.7.1.32
Alternative name(s):
CHETK-alpha
Ethanolamine kinase
Short name=EK
EC=2.7.1.82
Gene names
Name:Chka
Synonyms:Chk, Ckr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline By similarity.

Catalytic activity

ATP + choline = ADP + phosphocholine.

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Subunit structure

Homodimer, and heterodimer with CHKB By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Testis, brain, lung, kidney and liver.

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q01134-1)

Also known as: R2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q01134-2)

Also known as: R1;

The sequence of this isoform differs from the canonical sequence as follows:
     151-168: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Choline kinase alpha
PRO_0000206221

Regions

Nucleotide binding113 – 1197ATP By similarity
Nucleotide binding203 – 2097ATP By similarity
Region115 – 1173Substrate binding By similarity
Compositional bias53 – 8129Pro-rich

Sites

Binding site1421ATP By similarity
Binding site3041ATP By similarity
Binding site3261ATP By similarity

Natural variations

Alternative sequence151 – 16818Missing in isoform 2.
VSP_001067

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (R2) [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: DBF5643F321F0A2E

FASTA45351,824
        10         20         30         40         50         60 
MKTKFCTGGE AEPSPLGLLL SCGGSAAPTP GVGQQRDAAG ELESKQLGGR SQPLALPPPP 

        70         80         90        100        110        120 
PPPLPLPPPP SPPLADEQPE PRTRRRAYLW CKEFLPGAWR GLREDQFHIS VIRGGLSNML 

       130        140        150        160        170        180 
FQCSLPDSIA SVGDEPRKVL LRLYGAILKM RSCNKEGSEQ AQNENEFQGA EAMVLESVMF 

       190        200        210        220        230        240 
AILAERSLGP KLYGIFPQGR LEQFIPSRRL DTEELCLPDI SAEIAEKMAT FHGMKMPFNK 

       250        260        270        280        290        300 
EPKWLFGTME KYLNQVLRLK FSREARVQQL HKFLSYNLPL ELENLRSLLQ YTRSPVVFCH 

       310        320        330        340        350        360 
NDCQEGNILL LEGQENSEKQ KLMLIDFEYS SYNYRGFDIG NHFCEWMYDY TYEKYPFFRA 

       370        380        390        400        410        420 
NIQKYPTRKQ QLHFISSYLT TFQNDFESLS SEEQSATKED MLLEVNRFAL ASHFLWGLWS 

       430        440        450 
IVQAKISSIE FGYMEYAQAR FDAYFDQKRK LGV

« Hide

Isoform 2 (R1) [UniParc].

Checksum: A3EA4DD27652BE61
Show »

FASTA43549,744

References

[1]"Molecular cloning, characterization, and expression in Escherichia coli of a cDNA encoding mammalian choline kinase."
Uchida T., Yamashita S.
J. Biol. Chem. 267:10156-10162(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
Strain: Wistar.
Tissue: Liver.
[2]"Regulation of choline kinase R: analyses of alternatively spliced choline kinases and the promoter region."
Uchida T.
J. Biochem. 116:508-518(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
Strain: Sprague-Dawley and Wistar.
Tissue: Liver and Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D10261 mRNA. Translation: BAA01102.1.
D37884 Genomic DNA. Translation: BAA07126.1.
D37885 mRNA. Translation: BAA07127.1.
IPIIPI00199913.
IPI00230948.
PIRA42672.
JX0342.
RefSeqNP_058823.1. NM_017127.1.
UniGeneRn.10985.

3D structure databases

ProteinModelPortalQ01134.
SMRQ01134. Positions 83-453.
ModBaseSearch...

Proteomic databases

PaxDbQ01134.
PRIDEQ01134.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000022824; ENSRNOP00000022824; ENSRNOG00000016791.
ENSRNOT00000023020; ENSRNOP00000023020; ENSRNOG00000016791.
GeneID29194.
KEGGrno:29194.
UCSCRGD:61944. rat.

Organism-specific databases

CTD1119.
RGD61944. Chka.

Phylogenomic databases

eggNOGCOG0510.
GeneTreeENSGT00530000062991.
HOGENOMHOG000041274.
HOVERGENHBG050943.
InParanoidQ01134.
KOK14156.
OMATRRRAYL.
OrthoDBEOG49GKH0.

Enzyme and pathway databases

SABIO-RKQ01134.
UniPathwayUPA00558; UER00741.
UPA00753; UER00737.

Gene expression databases

ArrayExpressQ01134.
GenevestigatorQ01134.
GermOnlineENSRNOG00000016791. Rattus norvegicus.

Family and domain databases

InterProIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR22603. PTHR22603. 1 hit.
SUPFAMSSF56112. Kinase_like. 1 hit.
ProtoNetSearch...

Other

NextBio608327.

Entry information

Entry nameCHKA_RAT
AccessionPrimary (citable) accession number: Q01134
Secondary accession number(s): Q63114
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 30, 2000
Last modified: April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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