Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/arginine-rich splicing factor 2

Gene

SRSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.2 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • pre-mRNA binding Source: Ensembl
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161547-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiQ01130.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 2
Alternative name(s):
Protein PR264
Splicing component, 35 kDa
Splicing factor SC35
Short name:
SC-35
Splicing factor, arginine/serine-rich 2
Gene namesi
Name:SRSF2
Synonyms:SFRS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:10783. SRSF2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • interchromatin granule Source: Ensembl
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • spliceosomal complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6427.
OpenTargetsiENSG00000161547.
PharmGKBiPA35699.

Polymorphism and mutation databases

BioMutaiSRSF2.
DMDMi60416437.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000819182 – 221Serine/arginine-rich splicing factor 2Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei2PhosphoserineCombined sources1
Modified residuei22PhosphothreonineCombined sources1
Modified residuei25PhosphothreonineCombined sources1
Modified residuei26PhosphoserineCombined sources1
Modified residuei52N6-acetyllysine1 Publication1
Modified residuei189PhosphoserineCombined sources1
Modified residuei191PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation.2 Publications
Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ01130.
MaxQBiQ01130.
PaxDbiQ01130.
PeptideAtlasiQ01130.
PRIDEiQ01130.
TopDownProteomicsiQ01130-1. [Q01130-1]

PTM databases

iPTMnetiQ01130.
PhosphoSitePlusiQ01130.
SwissPalmiQ01130.

Expressioni

Inductioni

Accumulates in a hypoacetylated/phosphorylated form in response to cisplatin treatment.

Gene expression databases

BgeeiENSG00000161547.
CleanExiHS_SFRS2.
ExpressionAtlasiQ01130. baseline and differential.
GenevisibleiQ01130. HS.

Organism-specific databases

HPAiHPA049905.

Interactioni

Subunit structurei

Interacts with BRDT (By similarity). In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SCAF11. Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIR1Q86X954EBI-627047,EBI-627102
KAT5Q929933EBI-627047,EBI-399080

Protein-protein interaction databases

BioGridi112325. 110 interactors.
DIPiDIP-33836N.
IntActiQ01130. 54 interactors.
MINTiMINT-2863034.
STRINGi9606.ENSP00000353089.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 12Combined sources3
Beta strandi15 – 20Combined sources6
Helixi27 – 30Combined sources4
Helixi35 – 37Combined sources3
Beta strandi41 – 44Combined sources4
Beta strandi49 – 53Combined sources5
Beta strandi57 – 64Combined sources8
Helixi65 – 74Combined sources10
Beta strandi80 – 83Combined sources4
Beta strandi86 – 90Combined sources5
Beta strandi91 – 94Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KN4NMR-A9-101[»]
2LEANMR-A1-101[»]
2LEBNMR-A1-101[»]
2LECNMR-A1-101[»]
ProteinModelPortaliQ01130.
SMRiQ01130.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01130.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 92RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 116Gly-rich (hinge region)6
Compositional biasi117 – 221Arg/Ser-rich (RS domain)Add BLAST105

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4207. Eukaryota.
ENOG4111NJ8. LUCA.
GeneTreeiENSGT00700000104403.
HOVERGENiHBG107480.
InParanoidiQ01130.
KOiK12891.
OMAiNGSPEKQ.
OrthoDBiEOG091G13K0.
TreeFamiTF106262.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01130-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYGRPPPDV EGMTSLKVDN LTYRTSPDTL RRVFEKYGRV GDVYIPRDRY
60 70 80 90 100
TKESRGFAFV RFHDKRDAED AMDAMDGAVL DGRELRVQMA RYGRPPDSHH
110 120 130 140 150
SRRGPPPRRY GGGGYGRRSR SPRRRRRSRS RSRSRSRSRS RSRYSRSKSR
160 170 180 190 200
SRTRSRSRST SKSRSARRSK SKSSSVSRSR SRSRSRSRSR SPPPVSKRES
210 220
KSRSRSKSPP KSPEEEGAVS S
Length:221
Mass (Da):25,476
Last modified:January 23, 2007 - v4
Checksum:i68121AC4D35714FA
GO
Isoform 2 (identifier: Q01130-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-119: Missing.

Note: No experimental confirmation available.
Show »
Length:209
Mass (Da):24,153
Checksum:iE1229752981E5304
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38G → R in AAA60306 (PubMed:1373910).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056153108 – 119Missing in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90104 mRNA. Translation: AAA60306.1.
X62447 mRNA. Translation: CAA44307.1.
X75755 Genomic DNA. Translation: CAA53383.1.
BT007250 mRNA. Translation: AAP35914.1.
AK124792 mRNA. Translation: BAG54097.1.
AK297813 mRNA. Translation: BAG60151.1.
AC005837 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89439.1.
CH471099 Genomic DNA. Translation: EAW89448.1.
BC000339 mRNA. Translation: AAH00339.1.
BC001303 mRNA. Translation: AAH01303.1.
BC070086 mRNA. Translation: AAH70086.1.
L03693 Genomic DNA. Translation: AAA60162.1.
CCDSiCCDS11749.1. [Q01130-1]
RefSeqiNP_001182356.1. NM_001195427.1. [Q01130-1]
NP_003007.2. NM_003016.4. [Q01130-1]
XP_016880431.1. XM_017024942.1. [Q01130-1]
UniGeneiHs.584801.

Genome annotation databases

EnsembliENST00000359995; ENSP00000353089; ENSG00000161547. [Q01130-1]
ENST00000392485; ENSP00000376276; ENSG00000161547. [Q01130-1]
ENST00000452355; ENSP00000391278; ENSG00000161547. [Q01130-1]
ENST00000508921; ENSP00000441780; ENSG00000161547. [Q01130-2]
ENST00000585202; ENSP00000462425; ENSG00000161547. [Q01130-1]
GeneIDi6427.
KEGGihsa:6427.
UCSCiuc002jsv.4. human. [Q01130-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90104 mRNA. Translation: AAA60306.1.
X62447 mRNA. Translation: CAA44307.1.
X75755 Genomic DNA. Translation: CAA53383.1.
BT007250 mRNA. Translation: AAP35914.1.
AK124792 mRNA. Translation: BAG54097.1.
AK297813 mRNA. Translation: BAG60151.1.
AC005837 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89439.1.
CH471099 Genomic DNA. Translation: EAW89448.1.
BC000339 mRNA. Translation: AAH00339.1.
BC001303 mRNA. Translation: AAH01303.1.
BC070086 mRNA. Translation: AAH70086.1.
L03693 Genomic DNA. Translation: AAA60162.1.
CCDSiCCDS11749.1. [Q01130-1]
RefSeqiNP_001182356.1. NM_001195427.1. [Q01130-1]
NP_003007.2. NM_003016.4. [Q01130-1]
XP_016880431.1. XM_017024942.1. [Q01130-1]
UniGeneiHs.584801.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KN4NMR-A9-101[»]
2LEANMR-A1-101[»]
2LEBNMR-A1-101[»]
2LECNMR-A1-101[»]
ProteinModelPortaliQ01130.
SMRiQ01130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112325. 110 interactors.
DIPiDIP-33836N.
IntActiQ01130. 54 interactors.
MINTiMINT-2863034.
STRINGi9606.ENSP00000353089.

PTM databases

iPTMnetiQ01130.
PhosphoSitePlusiQ01130.
SwissPalmiQ01130.

Polymorphism and mutation databases

BioMutaiSRSF2.
DMDMi60416437.

Proteomic databases

EPDiQ01130.
MaxQBiQ01130.
PaxDbiQ01130.
PeptideAtlasiQ01130.
PRIDEiQ01130.
TopDownProteomicsiQ01130-1. [Q01130-1]

Protocols and materials databases

DNASUi6427.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359995; ENSP00000353089; ENSG00000161547. [Q01130-1]
ENST00000392485; ENSP00000376276; ENSG00000161547. [Q01130-1]
ENST00000452355; ENSP00000391278; ENSG00000161547. [Q01130-1]
ENST00000508921; ENSP00000441780; ENSG00000161547. [Q01130-2]
ENST00000585202; ENSP00000462425; ENSG00000161547. [Q01130-1]
GeneIDi6427.
KEGGihsa:6427.
UCSCiuc002jsv.4. human. [Q01130-1]

Organism-specific databases

CTDi6427.
DisGeNETi6427.
GeneCardsiSRSF2.
HGNCiHGNC:10783. SRSF2.
HPAiHPA049905.
MIMi600813. gene.
neXtProtiNX_Q01130.
OpenTargetsiENSG00000161547.
PharmGKBiPA35699.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4207. Eukaryota.
ENOG4111NJ8. LUCA.
GeneTreeiENSGT00700000104403.
HOVERGENiHBG107480.
InParanoidiQ01130.
KOiK12891.
OMAiNGSPEKQ.
OrthoDBiEOG091G13K0.
TreeFamiTF106262.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161547-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiQ01130.

Miscellaneous databases

ChiTaRSiSRSF2. human.
EvolutionaryTraceiQ01130.
GeneWikiiSFRS2.
GenomeRNAii6427.
PROiQ01130.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161547.
CleanExiHS_SFRS2.
ExpressionAtlasiQ01130. baseline and differential.
GenevisibleiQ01130. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF2_HUMAN
AccessioniPrimary (citable) accession number: Q01130
Secondary accession number(s): B3KWD5, B4DN89, H0YG49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 199 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.