Q01102 (LYAM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P-selectin

Alternative name(s):
CD62 antigen-like family member P
Granule membrane protein 140
Short name=GMP-140
Leukocyte-endothelial cell adhesion molecule 3
Short name=LECAM3
Platelet activation dependent granule-external membrane protein
Short name=PADGEM
CD_antigen=CD62P

Gene names

Name:	Selp
Synonyms:	Grmp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Ref.4
Subunit structure	Interacts with SNX17. Interacts with PSGL1/SEPL and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of PSGL1 and PODXL2, and specific tyrosine sulfation on PSGL1 By similarity. Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Induction	By TNF-alpha. Induced expression in lung, liver, kidney and heart after endotoxin treatment. Ref.1 Ref.2
Sequence similarities	Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 8 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Domain	EGF-like domain Repeat Signal Sushi Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to interleukin-6 Inferred from sequence orthology PubMed 9486531. Source: MGI heterophilic cell-cell adhesion Inferred from mutant phenotype PubMed 8557754PubMed 8562500. Source: MGI inflammatory response Inferred from mutant phenotype PubMed 7688665. Source: MGI leukocyte tethering or rolling Inferred from mutant phenotype PubMed 15956287. Source: MGI positive regulation of leukocyte migration Inferred from mutant phenotype PubMed 17114491. Source: MGI positive regulation of phosphatidylinositol 3-kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of platelet activation Inferred from mutant phenotype PubMed 16514062. Source: BHF-UCL regulation of integrin activation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 17114491PubMed 19349303PubMed 8562500. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: Compara platelet alpha granule membrane Inferred from electronic annotation. Source: Compara
Molecular_function	calcium-dependent protein binding Inferred from direct assay PubMed 7524641. Source: MGI fucose binding Inferred from electronic annotation. Source: Compara glycoprotein binding Inferred from direct assay PubMed 8562500. Source: MGI heparin binding Inferred from electronic annotation. Source: Compara lipopolysaccharide binding Inferred from electronic annotation. Source: Compara oligosaccharide binding Inferred from electronic annotation. Source: Compara sialic acid binding Inferred from direct assay PubMed 8562500. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 768	727	P-selectin		PRO_0000017499
Regions
	Topological domain	42 – 709	668	Extracellular Potential
	Transmembrane	710 – 733	24	Helical; Potential
	Topological domain	734 – 768	35	Cytoplasmic Potential
	Domain	58 – 158	101	C-type lectin
	Domain	159 – 195	37	EGF-like
	Domain	198 – 259	62	Sushi 1
	Domain	260 – 321	62	Sushi 2
	Domain	322 – 383	62	Sushi 3
	Domain	384 – 445	62	Sushi 4
	Domain	446 – 507	62	Sushi 5
	Domain	508 – 569	62	Sushi 6
	Domain	578 – 639	62	Sushi 7
	Domain	640 – 701	62	Sushi 8
	Region	759 – 768	10	Interaction with SNX17 By similarity
	Motif	756 – 759	4	Endocytosis signal Probable
Amino acid modifications
	Lipidation	745	1	S-palmitoyl cysteine By similarity
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	603	1	N-linked (GlcNAc...) Potential
	Glycosylation	654	1	N-linked (GlcNAc...) Potential
	Glycosylation	661	1	N-linked (GlcNAc...) Potential
	Glycosylation	679	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 158		By similarity
	Disulfide bond	131 ↔ 150		By similarity
	Disulfide bond	163 ↔ 174		By similarity
	Disulfide bond	168 ↔ 183		By similarity
	Disulfide bond	185 ↔ 194		By similarity
	Disulfide bond	200 ↔ 244		By similarity
	Disulfide bond	230 ↔ 257		By similarity
	Disulfide bond	262 ↔ 306		By similarity
	Disulfide bond	292 ↔ 319		By similarity
	Disulfide bond	324 ↔ 368		By similarity
	Disulfide bond	354 ↔ 381		By similarity
	Disulfide bond	386 ↔ 430		By similarity
	Disulfide bond	416 ↔ 443		By similarity
	Disulfide bond	448 ↔ 492		By similarity
	Disulfide bond	478 ↔ 505		By similarity
	Disulfide bond	510 ↔ 554		By similarity
	Disulfide bond	540 ↔ 567		By similarity
	Disulfide bond	580 ↔ 624		By similarity
	Disulfide bond	610 ↔ 637		By similarity
	Disulfide bond	642 ↔ 686		By similarity
	Disulfide bond	672 ↔ 699		By similarity
Experimental info
	Sequence conflict	724	1	A → E in AAA37712. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01102 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E5173074D2F66E68	FASTA	768	83,099
	10 20 30 40 50 60 MAGCPKGSWT PRLRSVILGG AQLIWFSALI SELVNQKEVA AWTYNYSTKA YSWNNSRVFC 70 80 90 100 110 120 RRHFTDLVAI QNKNEIAHLN DVIPFFNSYY WIGIRKINNK WTWVGTNKTL TEEAENWADN 130 140 150 160 170 180 EPNNKKNNQD CVEIYIKSNS APGKWNDEPC FKRKRALCYT ASCQDMSCSN QGECIETIGS 190 200 210 220 230 240 YTCSCYPGFY GPECEYVKEC GKVNIPQHVL MNCSHPLGEF SFNSQCTFSC AEGYELDGPG 250 260 270 280 290 300 ELQCLASGIW TNNPPKCDAV QCQSLEAPPH GTMACMHPIA AFAYDSSCKF ECQPGYRARG 310 320 330 340 350 360 SNTLHCTGSG QWSEPLPTCE AIACEPPEIP IHGSMDCVPS TGTFGYNSSC TFLCAEGFVL 370 380 390 400 410 420 KGNDAIQCAD SGQWTAPAPF CEALQCPEFP VPSKAQVNCS DPFGTLTYQS VCSFSCDEGS 430 440 450 460 470 480 LLVGASVIRC LATGHWNGAP PECQAVSCAP MLSPENGSMT CVQPLGNSTY KSTCQFMCDE 490 500 510 520 530 540 GFYLSGPERL DCSPSGHWTG TPPTCEAIKC PGIFAPEQGN LDCSHVHGEF GVGSICHFSC 550 560 570 580 590 600 NEDFELLGSE NVECTVSGRW SAPPPTCKGI TSLPAPAVRC PALTTPGQGT MSCQHHLGSF 610 620 630 640 650 660 GPNTTCYFGC KTGFTLRGAN SLRCRASGQW TAVTPMCRAV KCSELHMDTA VAMNCSNPWG 670 680 690 700 710 720 NFSYGSTCTF QCPEGQSLNG SVRATCREDG HWSDAMPTCQ AGTLTIQEAL TYLGGAVAST 730 740 750 760 TGLAVGGTLL ALLRKRLRKK DDGKCPLNPH SHLGTYGVFT NAAYDPTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha." Weller A., Isenmann S., Vestweber D. J. Biol. Chem. 267:15176-15183(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]	"Molecular cloning and analysis of in vivo expression of murine P-selectin." Sanders W.E. Jr., Wilson R.W., Ballantyne C.M., Beaudet A.L. Blood 80:795-800(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin." Hirata T., Furie B.C., Furie B. J. Immunol. 169:4307-4313(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"N-terminal residues in murine P-selectin glycoprotein ligand-1 required for binding to murine P-selectin." Xia L., Ramachandran V., McDaniel J.M., Nguyen K.N., Cummings R.D., McEver R.P. Blood 101:552-559(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SELPLG.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

P-selectin

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M87861 mRNA. Translation: AAA40008.1.
M72332 mRNA. Translation: AAA37712.1.
BC109158 mRNA. Translation: AAI09159.1.
BC109159 mRNA. Translation: AAI09160.1.

IPI

IPI00230123.

PIR

A42755.

RefSeq

NP_035477.1. NM_011347.2.

UniGene

Mm.3337.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GXB	X-ray	1.80	B	735-768	[»]

ProteinModelPortal

Q01102.

SMR

Q01102. Positions 42-199, 217-702.

ModBase

Search...

PTM databases

PhosphoSite

Q01102.

Proteomic databases

PRIDE

Q01102.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000162746; ENSMUSP00000123924; ENSMUSG00000026580.

GeneID

20344.

KEGG

mmu:20344.

UCSC

uc007dhz.1. mouse.

Organism-specific databases

CTD

6403.

MGI

MGI:98280. Selp.

Phylogenomic databases

eggNOG

NOG242963.

GeneTree

ENSGT00700000104229.

HOGENOM

HOG000236254.

HOVERGEN

HBG052375.

InParanoid

Q32MF1.

KO

K06496.

OMA

GQWTAVT.

OrthoDB

EOG45TCN6.

Gene expression databases

ArrayExpress

Q01102.

Bgee

Q01102.

CleanEx

MM_SELP.

Genevestigator

Q01102.

GermOnline

ENSMUSG00000026580. Mus musculus.

Family and domain databases

Gene3D

3.10.100.10. 1 hit.

InterPro

IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP.
[Graphical view]

Pfam

PF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 8 hits.
[Graphical view]

PRINTS

PR00343. SELECTIN.

SMART

SM00032. CCP. 8 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]

SUPFAM

SSF56436. C-type_lectin_fold. 1 hit.
SSF57535. Complement_control_module. 8 hits.

PROSITE

PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 8 hits.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q01102.

ChEMBL

CHEMBL2455.

NextBio

298177.

SOURCE

Search...

Entry information

Entry name

LYAM3_MOUSE

Accession

Primary (citable) accession number: Q01102
Secondary accession number(s): Q32MF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program