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Protein

P-selectin

Gene

Selp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: MGI
  • calcium ion binding Source: InterPro
  • fucose binding Source: MGI
  • glycoprotein binding Source: MGI
  • heparin binding Source: MGI
  • lipopolysaccharide binding Source: MGI
  • oligosaccharide binding Source: MGI
  • sialic acid binding Source: MGI

GO - Biological processi

  • calcium-mediated signaling using intracellular calcium source Source: MGI
  • cellular response to interleukin-6 Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  • inflammatory response Source: MGI
  • leukocyte cell-cell adhesion Source: MGI
  • leukocyte migration Source: MGI
  • leukocyte tethering or rolling Source: MGI
  • positive regulation of leukocyte migration Source: MGI
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
  • positive regulation of platelet activation Source: BHF-UCL
  • regulation of integrin activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-202733. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
P-selectin
Alternative name(s):
CD62 antigen-like family member P
Granule membrane protein 140
Short name:
GMP-140
Leukocyte-endothelial cell adhesion molecule 3
Short name:
LECAM3
Platelet activation dependent granule-external membrane protein
Short name:
PADGEM
CD_antigen: CD62P
Gene namesi
Name:Selp
Synonyms:Grmp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:98280. Selp.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini42 – 709ExtracellularSequence analysisAdd BLAST668
Transmembranei710 – 733HelicalSequence analysisAdd BLAST24
Topological domaini734 – 768CytoplasmicSequence analysisAdd BLAST35

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2455.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Sequence analysisAdd BLAST41
ChainiPRO_000001749942 – 768P-selectinAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 158By similarity
Disulfide bondi131 ↔ 150By similarity
Disulfide bondi163 ↔ 174By similarity
Disulfide bondi168 ↔ 183By similarity
Disulfide bondi185 ↔ 194By similarity
Disulfide bondi200 ↔ 244By similarity
Disulfide bondi230 ↔ 257By similarity
Disulfide bondi262 ↔ 306By similarity
Disulfide bondi292 ↔ 319By similarity
Disulfide bondi324 ↔ 368By similarity
Disulfide bondi354 ↔ 381By similarity
Disulfide bondi386 ↔ 430By similarity
Glycosylationi398N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi416 ↔ 443By similarity
Disulfide bondi448 ↔ 492By similarity
Disulfide bondi478 ↔ 505By similarity
Disulfide bondi510 ↔ 554By similarity
Disulfide bondi540 ↔ 567By similarity
Disulfide bondi580 ↔ 624By similarity
Glycosylationi603N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi610 ↔ 637By similarity
Disulfide bondi642 ↔ 686By similarity
Glycosylationi654N-linked (GlcNAc...)Sequence analysis1
Glycosylationi661N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi672 ↔ 699By similarity
Glycosylationi679N-linked (GlcNAc...)Sequence analysis1
Lipidationi745S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ01102.
PaxDbiQ01102.
PeptideAtlasiQ01102.
PRIDEiQ01102.

PTM databases

iPTMnetiQ01102.
PhosphoSitePlusiQ01102.

Expressioni

Tissue specificityi

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Inductioni

By TNF-alpha. Induced expression in lung, liver, kidney and heart after endotoxin treatment.2 Publications

Gene expression databases

BgeeiENSMUSG00000026580.
CleanExiMM_SELP.
ExpressionAtlasiQ01102. baseline and differential.
GenevisibleiQ01102. MM.

Interactioni

Subunit structurei

Interacts with SNX17. Interacts with PSGL1/SEPL and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of PSGL1 and PODXL2, and specific tyrosine sulfation on PSGL1 (By similarity).By similarity

GO - Molecular functioni

  • calcium-dependent protein binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123924.

Chemistry databases

BindingDBiQ01102.

Structurei

Secondary structure

1768
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi756 – 760Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GXBX-ray1.80B735-768[»]
ProteinModelPortaliQ01102.
SMRiQ01102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 158C-type lectinPROSITE-ProRule annotationAdd BLAST101
Domaini159 – 195EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini198 – 259Sushi 1PROSITE-ProRule annotationAdd BLAST62
Domaini260 – 321Sushi 2PROSITE-ProRule annotationAdd BLAST62
Domaini322 – 383Sushi 3PROSITE-ProRule annotationAdd BLAST62
Domaini384 – 445Sushi 4PROSITE-ProRule annotationAdd BLAST62
Domaini446 – 507Sushi 5PROSITE-ProRule annotationAdd BLAST62
Domaini508 – 569Sushi 6PROSITE-ProRule annotationAdd BLAST62
Domaini578 – 639Sushi 7PROSITE-ProRule annotationAdd BLAST62
Domaini640 – 701Sushi 8PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni759 – 768Interaction with SNX17By similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi756 – 759Endocytosis signalCurated4

Sequence similaritiesi

Belongs to the selectin/LECAM family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 8 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS44. Eukaryota.
ENOG410Y5JF. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000236254.
HOVERGENiHBG052375.
InParanoidiQ01102.
KOiK06496.
OMAiTYGVFTN.
OrthoDBiEOG091G00VV.
PhylomeDBiQ01102.
TreeFamiTF326910.

Family and domain databases

CDDicd00033. CCP. 8 hits.
Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 8 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 8 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 8 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCPKGSWT PRLRSVILGG AQLIWFSALI SELVNQKEVA AWTYNYSTKA
60 70 80 90 100
YSWNNSRVFC RRHFTDLVAI QNKNEIAHLN DVIPFFNSYY WIGIRKINNK
110 120 130 140 150
WTWVGTNKTL TEEAENWADN EPNNKKNNQD CVEIYIKSNS APGKWNDEPC
160 170 180 190 200
FKRKRALCYT ASCQDMSCSN QGECIETIGS YTCSCYPGFY GPECEYVKEC
210 220 230 240 250
GKVNIPQHVL MNCSHPLGEF SFNSQCTFSC AEGYELDGPG ELQCLASGIW
260 270 280 290 300
TNNPPKCDAV QCQSLEAPPH GTMACMHPIA AFAYDSSCKF ECQPGYRARG
310 320 330 340 350
SNTLHCTGSG QWSEPLPTCE AIACEPPEIP IHGSMDCVPS TGTFGYNSSC
360 370 380 390 400
TFLCAEGFVL KGNDAIQCAD SGQWTAPAPF CEALQCPEFP VPSKAQVNCS
410 420 430 440 450
DPFGTLTYQS VCSFSCDEGS LLVGASVIRC LATGHWNGAP PECQAVSCAP
460 470 480 490 500
MLSPENGSMT CVQPLGNSTY KSTCQFMCDE GFYLSGPERL DCSPSGHWTG
510 520 530 540 550
TPPTCEAIKC PGIFAPEQGN LDCSHVHGEF GVGSICHFSC NEDFELLGSE
560 570 580 590 600
NVECTVSGRW SAPPPTCKGI TSLPAPAVRC PALTTPGQGT MSCQHHLGSF
610 620 630 640 650
GPNTTCYFGC KTGFTLRGAN SLRCRASGQW TAVTPMCRAV KCSELHMDTA
660 670 680 690 700
VAMNCSNPWG NFSYGSTCTF QCPEGQSLNG SVRATCREDG HWSDAMPTCQ
710 720 730 740 750
AGTLTIQEAL TYLGGAVAST TGLAVGGTLL ALLRKRLRKK DDGKCPLNPH
760
SHLGTYGVFT NAAYDPTP
Length:768
Mass (Da):83,099
Last modified:April 1, 1993 - v1
Checksum:iE5173074D2F66E68
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti724A → E in AAA37712 (PubMed:1379089).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87861 mRNA. Translation: AAA40008.1.
M72332 mRNA. Translation: AAA37712.1.
BC109158 mRNA. Translation: AAI09159.1.
BC109159 mRNA. Translation: AAI09160.1.
CCDSiCCDS48422.1.
PIRiA42755.
RefSeqiNP_035477.1. NM_011347.2.
UniGeneiMm.3337.

Genome annotation databases

EnsembliENSMUST00000162746; ENSMUSP00000123924; ENSMUSG00000026580.
GeneIDi20344.
KEGGimmu:20344.
UCSCiuc007dhz.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

P-selectin

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87861 mRNA. Translation: AAA40008.1.
M72332 mRNA. Translation: AAA37712.1.
BC109158 mRNA. Translation: AAI09159.1.
BC109159 mRNA. Translation: AAI09160.1.
CCDSiCCDS48422.1.
PIRiA42755.
RefSeqiNP_035477.1. NM_011347.2.
UniGeneiMm.3337.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GXBX-ray1.80B735-768[»]
ProteinModelPortaliQ01102.
SMRiQ01102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123924.

Chemistry databases

BindingDBiQ01102.
ChEMBLiCHEMBL2455.

PTM databases

iPTMnetiQ01102.
PhosphoSitePlusiQ01102.

Proteomic databases

MaxQBiQ01102.
PaxDbiQ01102.
PeptideAtlasiQ01102.
PRIDEiQ01102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162746; ENSMUSP00000123924; ENSMUSG00000026580.
GeneIDi20344.
KEGGimmu:20344.
UCSCiuc007dhz.2. mouse.

Organism-specific databases

CTDi6403.
MGIiMGI:98280. Selp.

Phylogenomic databases

eggNOGiENOG410IS44. Eukaryota.
ENOG410Y5JF. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000236254.
HOVERGENiHBG052375.
InParanoidiQ01102.
KOiK06496.
OMAiTYGVFTN.
OrthoDBiEOG091G00VV.
PhylomeDBiQ01102.
TreeFamiTF326910.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-202733. Cell surface interactions at the vascular wall.

Miscellaneous databases

PROiQ01102.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026580.
CleanExiMM_SELP.
ExpressionAtlasiQ01102. baseline and differential.
GenevisibleiQ01102. MM.

Family and domain databases

CDDicd00033. CCP. 8 hits.
Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 8 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 8 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 8 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 8 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLYAM3_MOUSE
AccessioniPrimary (citable) accession number: Q01102
Secondary accession number(s): Q32MF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.