Q01098 (NMDE3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor ionotropic, NMDA 2C
Short name=GluN2C

Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-3
N-methyl D-aspartate receptor subtype 2C
Short name=NMDAR2C
Short name=NR2C

Gene names

Name:

Grin2c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Subunit structure	Interacts with PDZ domains of INADL and DLG4 By similarity. Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Calcium Magnesium
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	directional locomotion Inferred from genetic interaction PubMed 8987814. Source: MGI negative regulation of protein catabolic process Inferred from genetic interaction PubMed 15317856. Source: MGI neuromuscular process controlling balance Inferred from mutant phenotype PubMed 9458051. Source: MGI protein localization Inferred from mutant phenotype PubMed 15317856. Source: MGI regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype PubMed 8756432PubMed 8987814. Source: MGI response to wounding Inferred from genetic interaction PubMed 9512392. Source: MGI
Cellular_component	N-methyl-D-aspartate selective glutamate receptor complex Inferred from physical interaction PubMed 7929101. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: Compara postsynaptic density Inferred from direct assay PubMed 15317856. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-methyl-D-aspartate selective glutamate receptor activity Inferred from mutant phenotype PubMed 8756432PubMed 9427357PubMed 9512392. Source: MGI cation channel activity Inferred from mutant phenotype PubMed 8756432PubMed 9427357. Source: MGI extracellular-glutamate-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 1239	1220	Glutamate receptor ionotropic, NMDA 2C		PRO_0000011581
Regions
	Topological domain	20 – 553	534	Extracellular Potential
	Transmembrane	554 – 574	21	Helical; Potential
	Topological domain	575 – 626	52	Cytoplasmic Potential
	Transmembrane	627 – 647	21	Helical; Potential
	Topological domain	648 – 814	167	Extracellular Potential
	Transmembrane	815 – 835	21	Helical; Potential
	Topological domain	836 – 1239	404	Cytoplasmic Potential
	Motif	1237 – 1239	3	PDZ-binding By similarity
Sites
	Site	612	1	Functional determinant of NMDA receptors By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Glycosylation	539	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01098 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 793E8E731E20C3C9	FASTA	1,239	135,420
	10 20 30 40 50 60 MGGALGPALL LTSLLGAWAG LGAGQGEQAV TVAVVFGSSG PLQAQARTRL TPQNFLDLPL 70 80 90 100 110 120 EIQPLTIGVN NTNPSSILTQ ICGLLGAARV HGIVFEDNVD TEAVAQLLDF VSSQTHVPIL 130 140 150 160 170 180 SISGGSAVVL TPKEPGSAFL QLGVSLEQQL QVLFKVLEEY DWSAFAVITS LHPGHALFLE 190 200 210 220 230 240 GVRAVADASY LSWRLLDVLT LELGPGGPRA RTQRLLRQVD APVLVAYCSR EEAEVLFAEA 250 260 270 280 290 300 AQAGLVGPGH VWLVPNLALG STDAPPAAFP VGLISVVTES WRLSLRQKVR DGVAILALGA 310 320 330 340 350 360 HSYRRQYGTL PAPAGDCRSH PGPVSPAREA FYRHLLNVTW EGRDFSFSPG GYLVQPTMVV 370 380 390 400 410 420 IALNRHRLWE MVGRWDHGVL YMKYPVWPRY STSLQPVVDS RHLTVATLEE RPFVIVESPD 430 440 450 460 470 480 PGTGGCVPNT VPCRRQSNHT FSSGDITPYT KLCCKGFCID ILKKLAKVVK FSYDLYLVTN 490 500 510 520 530 540 GKHGKRVRGV WNGMIGEVYY KRADMAIGSL TINEERSEII DFSVPFVETG ISVMVARSNG 550 560 570 580 590 600 TVSPSAFLEP YSPAVWVMMF VMCLTVVAIT VFMFEYFSPV SYNQNLTKGK KSGGPSFTIG 610 620 630 640 650 660 KSVWLLWALV FNNSVPIENP RGTTSKIMVL VWAFFAVIFL ASYTANLAAF MIQEQYIDTV 670 680 690 700 710 720 SGLSDKKFQR PQDQYPPFRF GTVPNGSTER NIRSNYRDMH THMVKFNQRS VEDALTSLKM 730 740 750 760 770 780 GKLDAFIYDA AVLNYMAGKD EGCKLVTIGS GKVFATTGYG IAMQKDSHWK RAIDLALLQF 790 800 810 820 830 840 LGDGETQKLE TVWLSGICHN EKNEVMSSKL DIDNMAGVFY MLLVAMGLAL LVFAWEHLVY 850 860 870 880 890 900 WKLRHSVPSS SQLDFLLAFS RGIYSCFNGV QSLPSPARPP SPDLTAGSAQ ANVLKMLQAA 910 920 930 940 950 960 RDMVSTADVS GSLDRATRTI ENWGNNRRAP APTTSGPRSC TPGPPGQPSP SGWRPPGGGR 970 980 990 1000 1010 1020 TPLARRAPQP PARPATCAGS PQPDVSRASC RHAWDARWPV RVGHQGSHLS ASERRALPER 1030 1040 1050 1060 1070 1080 SLLHAHCHYS SFPRAERSGR PFLPLFPEPP EPDDLPLLGP EQLARREALL RAAWARGPRP 1090 1100 1110 1120 1130 1140 RHASLPSSVA EAFTRSNPLP ARCTGHACAC PCPQSRPSCR HVAQTQSLRL PSYREACVEG 1150 1160 1170 1180 1190 1200 VPAGVAATWQ PRQHVCLHTH THLPFCWGTV CRHPPPCSSH SPWLIGTWEP PSHRGRTLGL 1210 1220 1230 GTGYRDSGVL EEVSREACGT QGFPRSCTWR RISSLESEV

References

[1]	"Molecular diversity of the NMDA receptor channel." Kutsuwada T., Kashiwabuchi N., Mori H., Sakimura K., Kushiya E., Araki K., Meguro H., Masaki H., Kumanishi T., Arakawa M., Mishina M. Nature 358:36-41(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Kashiwabuchi N. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10694 mRNA. Translation: BAA01536.1.
IPI	IPI00321321.
PIR	I49705.
RefSeq	NP_034480.2. NM_010350.2.
UniGene	Mm.39090.
3D structure databases
ProteinModelPortal	Q01098.
SMR	Q01098. Positions 67-832.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01098. 1 interaction.
MINT	MINT-104161.
PTM databases
PhosphoSite	Q01098.
Proteomic databases
PaxDb	Q01098.
PRIDE	Q01098.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003351; ENSMUSP00000003351; ENSMUSG00000020734. ENSMUST00000106554; ENSMUSP00000102164; ENSMUSG00000020734.
GeneID	14813.
KEGG	mmu:14813.
UCSC	uc007mgx.1. mouse.
Organism-specific databases
CTD	2905.
MGI	MGI:95822. Grin2c.
Phylogenomic databases
eggNOG	NOG282132.
GeneTree	ENSGT00540000070221.
HOGENOM	HOG000231061.
HOVERGEN	HBG052636.
InParanoid	Q01098.
KO	K05211.
OMA	CTWRRIS.
OrthoDB	EOG42RD6M.
Gene expression databases
ArrayExpress	Q01098.
Bgee	Q01098.
Genevestigator	Q01098.
GermOnline	ENSMUSG00000020734. Mus musculus.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR019594. Glu_rcpt_Glu/Gly-bd. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. IPR018884. NMDAR2_C. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00060. Lig_chan. 1 hit. PF10613. Lig_chan-Glu_bd. 1 hit. PF10565. NMDAR2_C. 1 hit. [Graphical view]
PRINTS	PR00177. NMDARECEPTOR.
SMART	SM00918. Lig_chan-Glu_bd. 1 hit. SM00079. PBPe. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q01098.
ChEMBL	CHEMBL3555.
NextBio	287007.
SOURCE	Search...

Entry information

Entry name

NMDE3_MOUSE

Accession

Primary (citable) accession number: Q01098

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program