Q01085 (TIAR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolysin TIAR

Alternative name(s):
TIA-1-related protein

Gene names

Name:

TIAL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Cytoplasmic granule. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules By similarity. Cytoplasmic granules of cytolytic T-lymphocytes.
Post-translational modification	Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. Ref.5
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Traceable author statement Ref.1. Source: ProtInc germ cell development Inferred from electronic annotation. Source: Compara induction of apoptosis Traceable author statement Ref.1. Source: ProtInc positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 9207209. Source: ProtInc stem cell division Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasmic stress granule Inferred from sequence or structural similarity. Source: UniProtKB lysosome Traceable author statement Ref.1. Source: ProtInc nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	AU-rich element binding Inferred from electronic annotation. Source: Compara RNA binding Traceable author statement Ref.1. Source: ProtInc nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01085-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01085-2)

The sequence of this isoform differs from the canonical sequence as follows:
     43-43: E → EQPDSRRVNSSVGFSVLQ

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 375

375

Nucleolysin TIAR

PRO_0000081978

Regions

Domain

9 – 85

77

RRM 1

Domain

97 – 175

79

RRM 2

Domain

205 – 277

73

RRM 3

Amino acid modifications

Modified residue

122

1

N6-acetyllysine Ref.4

Modified residue

201

1

Phosphoserine Ref.6

Natural variations

Alternative sequence

43

1

E → EQPDSRRVNSSVGFSVLQ in isoform 2.

VSP_043700

Secondary structure

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375

Helix Strand Turn

Details...

Beta strand

10 – 15

6

Helix

22 – 32

11

Beta strand

35 – 41

7

Beta strand

50 – 57

8

Helix

58 – 68

11

Beta strand

71 – 73

3

Beta strand

76 – 82

7

Beta strand

97 – 102

6

Helix

110 – 116

7

Turn

118 – 120

3

Beta strand

123 – 130

8

Beta strand

132 – 134

3

Beta strand

136 – 147

12

Helix

148 – 157

10

Turn

158 – 160

3

Beta strand

164 – 166

3

Helix

193 – 199

7

Beta strand

206 – 210

5

Helix

218 – 228

11

Beta strand

231 – 237

7

Turn

238 – 241

4

Beta strand

242 – 249

8

Helix

250 – 260

11

Beta strand

271 – 273

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 2F8D1C7169A7817E	FASTA	375	41,591
	10 20 30 40 50 60 MMEDDGQPRT LYVGNLSRDV TEVLILQLFS QIGPCKSCKM ITEHTSNDPY CFVEFYEHRD 70 80 90 100 110 120 AAAALAAMNG RKILGKEVKV NWATTPSSQK KDTSNHFHVF VGDLSPEITT EDIKSAFAPF 130 140 150 160 170 180 GKISDARVVK DMATGKSKGY GFVSFYNKLD AENAIVHMGG QWLGGRQIRT NWATRKPPAP 190 200 210 220 230 240 KSTQENNTKQ LRFEDVVNQS SPKNCTVYCG GIASGLTDQL MRQTFSPFGQ IMEIRVFPEK 250 260 270 280 290 300 GYSFVRFSTH ESAAHAIVSV NGTTIEGHVV KCYWGKESPD MTKNFQQVDY SQWGQWSQVY 310 320 330 340 350 360 GNPQQYGQYM ANGWQVPPYG VYGQPWNQQG FGVDQSPSAA WMGGFGAQPP QGQAPPPVIP 370 PPNQAGYGMA SYQTQ
	Isoform 2 [UniParc]. Checksum: F81DA9563C041D75	FASTA	392	43,449
	10 20 30 40 50 60 MMEDDGQPRT LYVGNLSRDV TEVLILQLFS QIGPCKSCKM ITEQPDSRRV NSSVGFSVLQ 70 80 90 100 110 120 HTSNDPYCFV EFYEHRDAAA ALAAMNGRKI LGKEVKVNWA TTPSSQKKDT SNHFHVFVGD 130 140 150 160 170 180 LSPEITTEDI KSAFAPFGKI SDARVVKDMA TGKSKGYGFV SFYNKLDAEN AIVHMGGQWL 190 200 210 220 230 240 GGRQIRTNWA TRKPPAPKST QENNTKQLRF EDVVNQSSPK NCTVYCGGIA SGLTDQLMRQ 250 260 270 280 290 300 TFSPFGQIME IRVFPEKGYS FVRFSTHESA AHAIVSVNGT TIEGHVVKCY WGKESPDMTK 310 320 330 340 350 360 NFQQVDYSQW GQWSQVYGNP QQYGQYMANG WQVPPYGVYG QPWNQQGFGV DQSPSAAWMG 370 380 390 GFGAQPPQGQ APPPVIPPPN QAGYGMASYQ TQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and functional characterization of a TIA-1-related nucleolysin." Kawakami A., Tian Q., Duan X., Streuli M., Schlossman S.F., Anderson P. Proc. Natl. Acad. Sci. U.S.A. 89:8681-8685(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[3]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-122, MASS SPECTROMETRY.
[5]	"DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization." Reinhardt H.C., Hasskamp P., Schmedding I., Morandell S., van Vugt M.A., Wang X., Linding R., Ong S.E., Weaver D., Carr S.A., Yaffe M.B. Mol. Cell 40:34-49(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY MAPK14.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Solution structure of the RNA binding domains of nucleolysin TIAR." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-282.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M96954 mRNA. Translation: AAA36384.1.
AK290695 mRNA. Translation: BAF83384.1.
AK290984 mRNA. Translation: BAF83673.1.

IPI

IPI00005615.
IPI00644708.

PIR

A46174.

RefSeq

NP_001029097.1. NM_001033925.1.
NP_003243.1. NM_003252.3.

UniGene

Hs.501203.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X4G	NMR	-	A	187-282	[»]
2CQI	NMR	-	A	1-90	[»]
2DH7	NMR	-	A	89-180	[»]

ProteinModelPortal

Q01085.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-42425N.

IntAct

Q01085. 12 interactions.

MINT

MINT-1203913.

STRING

9606.ENSP00000358089.

PTM databases

PhosphoSite

Q01085.

Polymorphism databases

DMDM

267131.

Proteomic databases

PaxDb

Q01085.

PRIDE

Q01085.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000369093; ENSP00000358089; ENSG00000151923.
ENST00000436547; ENSP00000394902; ENSG00000151923.

GeneID

7073.

KEGG

hsa:7073.

UCSC

uc001leh.1. human.
uc001lei.1. human.

Organism-specific databases

CTD

7073.

GeneCards

GC10M121324.

HGNC

HGNC:11804. TIAL1.

MIM

603413. gene.

neXtProt

NX_Q01085.

PharmGKB

PA36513.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0724.

HOGENOM

HOG000206748.

HOVERGEN

HBG105006.

KO

K13201.

OMA

EEVYNQS.

OrthoDB

EOG4B5P59.

PhylomeDB

Q01085.

Gene expression databases

ArrayExpress

Q01085.

Bgee

Q01085.

CleanEx

HS_TIAL1.

Genevestigator

Q01085.

GermOnline

ENSG00000151923. Homo sapiens.

Family and domain databases

Gene3D

3.30.70.330. 3 hits.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 3 hits.
[Graphical view]

SMART

SM00360. RRM. 3 hits.
[Graphical view]

PROSITE

PS50102. RRM. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TIAL1. human.

EvolutionaryTrace

Q01085.

GenomeRNAi

7073.

NextBio

27661.

SOURCE

Search...

Entry information

Entry name

TIAR_HUMAN

Accession

Primary (citable) accession number: Q01085
Secondary accession number(s): A8K3T0, A8K4L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 29, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.