Reviewed, UniProtKB/Swiss-Prot Q01077 (G3P2_KLUMA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 2
      Short name=GAPDH 2
    EC=1.2.1.12

Gene names

Name:

GAP2

Organism

Kluyveromyces marxianus (Yeast) (Candida kefyr)

Taxonomic identifier

4911 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Glyceraldehyde-3-phosphate dehydrogenase 2		PRO_0000145558
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	148 – 150	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	208 – 209	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	149	1	Nucleophile By similarity
	Binding site	33	1	NAD By similarity
	Binding site	78	1	NAD; via carbonyl oxygen By similarity
	Binding site	179	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	231	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	313	1	NAD By similarity
	Site	176	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01077-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: FEDB08479F4B5F09	FASTA	331	35,540
	10 20 30 40 50 60 MVRIAINGFG RIGRLVLRIA LSRKNIEVVA INDPFITVDY AAYMFKYDST HGRFDGEVSH 70 80 90 100 110 120 DGKSLIIDGK KVLVFQERDP ATLPWGAEKI DIAIDSTGIF KELDSAQKHI DAGAKKVVIT 130 140 150 160 170 180 APSSTAPMFV VGVNEDKYAG QTIVSNASCT TNCLAPLAKI INNAFGIEEG LMTTVHSITA 190 200 210 220 230 240 TQKTVDGPSH KDWRGGRTAS GNIIPSSTGA AKAVGKVLPE LQGKLTGMAF RVPTVDVSVV 250 260 270 280 290 300 DLTVKLAKPA TYEEIKAVVK KASENELKGV MGYTEDAVVS SDFLGDTHSS IFDAAAGIQL 310 320 330 SPQFVKLVSW YDNEFGYSTR VVDLVELVAK N

References

[1]

"Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene family from Kluyveromyces marxianus -- polymerase chain reaction-single-strand conformation polymorphism as a tool for the study of multigenic families." Fernandes P.A., Sena-Esteves M., Moradas-Ferreira P. Yeast 11:725-733(1995) [PubMed: 7668042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10022 / CBS 6432 / NCTC 2303 / NRRL Y-665.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S80241 Genomic DNA. Translation: AAB35209.1.
PIR	S57280.
3D structure databases
SMR	Q01077. Positions 1-329.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P2_KLUMA

Accession

Primary (citable) accession number: Q01077

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)