Reviewed, UniProtKB/Swiss-Prot Q01071 (ESMD_DROME)

Names and origin

Protein names

Recommended name:
    Enhancer of split mdelta protein
      Short name=E(spl)mdelta
Alternative name(s):
    HLH-mdelta
    Split locus enhancer protein mC

Gene names

Name:	HLHmdelta
ORF Names:	CG8328

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional repressor of genes that require a bHLH protein for their transcription. May serve as a transcriptional regulator of the Achaete-scute complex (AS-C) genes. Contributes to the neural-epidermal lineage decision during early neurogenesis. Part of the Notch signaling pathway.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein (Groucho) By similarity.
Subcellular location	Nucleus Probable.
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). Ref.8 The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins. Ref.8
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Notch signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW compound eye development Traceable author statement. Source: FlyBase nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8MSM9	1	EBI-118907,EBI-136178
gro	P16371	2	EBI-118907,EBI-153866
Osi6	Q9XZ15	1	EBI-118907,EBI-110025

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Enhancer of split mdelta protein		PRO_0000127178
Regions
	Domain	29 – 73	45	Helix-loop-helix motif
	Domain	93 – 126	34	Orange
	DNA binding	16 – 28	13	Basic motif
	Motif	170 – 173	4	WRPW motif
	Compositional bias	72 – 75	4	Poly-Gln
Natural variations
	Natural variant	121	1	H → R in strain: NVIII-42 and NVIII-18.
	Natural variant	162	1	D → V in strain: NVIII-m11.
Experimental info
	Sequence conflict	82	1	S → W in CAA47433. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01071-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F44DA6100CE0874F	FASTA	173	20,195
	10 20 30 40 50 60 MAVQGQRFMT KTQHYRKVTK PLLERKRRAR MNLYLDELKD LIVDTMDAQG EQVSKLEKAD 70 80 90 100 110 120 ILELTVNYLK AQQQQRVANP QSPPPDQVNL DKFRAGYTQA AYEVSHIFST VPGLDLKFGT 130 140 150 160 170 HLMKQLGHQL KDMKQEEEII DMAEEPVNLA DQKRSKSPRE EDIHHGEEVW RPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Seven genes of the Enhancer of split complex of Drosophila melanogaster encode helix-loop-helix proteins." Knust E., Schrons H., Grawe F., Campos-Ortega J.A. Genetics 132:505-518(1992) [PubMed: 1427040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R.
[2]	"The Enhancer of split [E(spl)] locus of Drosophila encodes seven independent helix-loop-helix proteins." Delidakis C., Artavanis-Tsakonas S. Proc. Natl. Acad. Sci. U.S.A. 89:8731-8735(1992) [PubMed: 1528887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Embryo.
[3]	"Identifying signatures of selection at the enhancer of split neurogenic gene complex in Drosophila." Macdonald S.J., Long A.D. Mol. Biol. Evol. 22:607-619(2005) [PubMed: 15537803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41, NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13, NVIII-m15 and NVIII-m19.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[6]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[7]	"A low-cost open-source SNP genotyping platform for association mapping applications." Macdonald S.J., Pastinen T., Genissel A., Cornforth T.W., Long A.D. Genome Biol. 6:RESEARCH105.1-RESEARCH105.11(2005) [PubMed: 16356268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-173. Strain: Nv2001_m0376, Nv2001_m0377, Nv2001_m0378, Nv2001_m0379, Nv2001_m0380, Nv2001_m0381, Nv2001_m0382, Nv2001_m0383, Nv2001_m0384, Nv2001_m0385, Nv2001_m0386, Nv2001_m0387, Nv2001_m0388, Nv2001_m0389, Nv2001_m0390, Nv2001_m0391, Nv2001_m0392, Nv2001_m0393, Nv2001_m0394, Nv2001_m0395, Nv2001_m0396, Nv2001_m0397, Nv2001_m0398, Nv2001_m0399, Nv2001_m0400, Nv2001_m0401, Nv2001_m0402, Nv2001_m0403, Nv2001_m0404, Nv2001_m0405, Nv2001_m0406, Nv2001_m0407, Nv2001_m0408, Nv2001_m0409, Nv2001_m0410, Nv2001_m0411, Nv2001_m0412, Nv2001_m0413, Nv2001_m0414, Nv2001_m0415, Nv2001_m0416, Nv2001_m0417, Nv2001_m0418, Nv2001_m0419, Nv2001_m0420, Nv2001_m0421, Nv2001_m0422, Nv2001_m0423, Nv2001_m0424, Nv2001_m0425, Nv2001_m0426, Nv2001_m0427, Nv2001_m0428, Nv2001_m0429, Nv2001_m0430, Nv2001_m0431, Nv2001_m0432, Nv2001_m0433, Nv2001_m0434, Nv2001_m0435, Nv2001_m0436, Nv2001_m0437, Nv2001_m0438, Nv2001_m0439, Nv2001_m0440, Nv2001_m0441, Nv2001_m0442, Nv2001_m0443, Nv2001_m0444, Nv2001_m0445, Nv2001_m0446, Nv2001_m0447, Nv2001_m0448, Nv2001_m0449, Nv2001_m0450, Nv2001_m0451, Nv2001_m0452, Nv2001_m0453, Nv2001_m0454, Nv2001_m0455, Nv2001_m0456, Nv2001_m0457, Nv2001_m0458, Nv2001_m0459, Nv2001_m0460, Nv2001_m0462, Nv2001_m0463, Nv2001_m0464, Nv2001_m0465, Nv2001_m0466, Nv2001_m0467, Nv2001_m0468 and Nv2001_m0469.
[8]	"Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins." Paroush Z., Finley R.L. Jr., Kidd T., Wainwright S.M., Ingham P.W., Brent R., Ish-Horowicz D. Cell 79:805-815(1994) [PubMed: 8001118] [Abstract] Cited for: DOMAIN WRPW MOTIF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67048 mRNA. Translation: CAA47433.1. M96168 Genomic DNA. Translation: AAA28911.1. AY779906 Genomic DNA. Translation: AAV59045.1. AY779907 Genomic DNA. Translation: AAV59057.1. AY779908 Genomic DNA. Translation: AAV59069.1. AY779909 Genomic DNA. Translation: AAV59081.1. AY779910 Genomic DNA. Translation: AAV59093.1. AY779911 Genomic DNA. Translation: AAV59105.1. AY779912 Genomic DNA. Translation: AAV59117.1. AY779913 Genomic DNA. Translation: AAV59129.1. AY779914 Genomic DNA. Translation: AAV59141.1. AY779915 Genomic DNA. Translation: AAV59153.1. AY779916 Genomic DNA. Translation: AAV59165.1. AY779917 Genomic DNA. Translation: AAV59177.1. AY779918 Genomic DNA. Translation: AAV59189.1. AY779919 Genomic DNA. Translation: AAV59201.1. AY779920 Genomic DNA. Translation: AAV59213.1. AY779921 Genomic DNA. Translation: AAV59225.1. AE014297 Genomic DNA. Translation: AAF56544.1. BT022133 mRNA. Translation: AAY51528.2. AY905719 Genomic DNA. Translation: AAX60065.1. AY905720 Genomic DNA. Translation: AAX60066.1. AY905721 Genomic DNA. Translation: AAX60067.1. AY905722 Genomic DNA. Translation: AAX60068.1. AY905723 Genomic DNA. Translation: AAX60069.1. AY905724 Genomic DNA. Translation: AAX60070.1. AY905725 Genomic DNA. Translation: AAX60071.1. AY905726 Genomic DNA. Translation: AAX60072.1. AY905727 Genomic DNA. Translation: AAX60073.1. AY905728 Genomic DNA. Translation: AAX60074.1. AY905729 Genomic DNA. Translation: AAX60075.1. AY905730 Genomic DNA. Translation: AAX60076.1. AY905731 Genomic DNA. Translation: AAX60077.1. AY905732 Genomic DNA. Translation: AAX60078.1. AY905733 Genomic DNA. Translation: AAX60079.1. AY905734 Genomic DNA. Translation: AAX60080.1. AY905735 Genomic DNA. Translation: AAX60081.1. AY905736 Genomic DNA. Translation: AAX60082.1. AY905737 Genomic DNA. Translation: AAX60083.1. AY905738 Genomic DNA. Translation: AAX60084.1. AY905739 Genomic DNA. Translation: AAX60085.1. AY905740 Genomic DNA. Translation: AAX60086.1. AY905741 Genomic DNA. Translation: AAX60087.1. AY905742 Genomic DNA. Translation: AAX60088.1. AY905743 Genomic DNA. Translation: AAX60089.1. AY905744 Genomic DNA. Translation: AAX60090.1. AY905745 Genomic DNA. Translation: AAX60091.1. AY905746 Genomic DNA. Translation: AAX60092.1. AY905747 Genomic DNA. Translation: AAX60093.1. AY905748 Genomic DNA. Translation: AAX60094.1. AY905749 Genomic DNA. Translation: AAX60095.1. AY905750 Genomic DNA. Translation: AAX60096.1. AY905751 Genomic DNA. Translation: AAX60097.1. AY905752 Genomic DNA. Translation: AAX60098.1. AY905753 Genomic DNA. Translation: AAX60099.1. AY905754 Genomic DNA. Translation: AAX60100.1. AY905755 Genomic DNA. Translation: AAX60101.1. AY905756 Genomic DNA. Translation: AAX60102.1. AY905757 Genomic DNA. Translation: AAX60103.1. AY905758 Genomic DNA. Translation: AAX60104.1. AY905759 Genomic DNA. Translation: AAX60105.1. AY905760 Genomic DNA. Translation: AAX60106.1. AY905761 Genomic DNA. Translation: AAX60107.1. AY905762 Genomic DNA. Translation: AAX60108.1. AY905763 Genomic DNA. Translation: AAX60109.1. AY905764 Genomic DNA. Translation: AAX60110.1. AY905765 Genomic DNA. Translation: AAX60111.1. AY905766 Genomic DNA. Translation: AAX60112.1. AY905767 Genomic DNA. Translation: AAX60113.1. AY905768 Genomic DNA. Translation: AAX60114.1. AY905769 Genomic DNA. Translation: AAX60115.1. AY905770 Genomic DNA. Translation: AAX60116.1. AY905771 Genomic DNA. Translation: AAX60117.1. AY905772 Genomic DNA. Translation: AAX60118.1. AY905773 Genomic DNA. Translation: AAX60119.1. AY905774 Genomic DNA. Translation: AAX60120.1. AY905775 Genomic DNA. Translation: AAX60121.1. AY905776 Genomic DNA. Translation: AAX60122.1. AY905777 Genomic DNA. Translation: AAX60123.1. AY905778 Genomic DNA. Translation: AAX60124.1. AY905779 Genomic DNA. Translation: AAX60125.1. AY905780 Genomic DNA. Translation: AAX60126.1. AY905781 Genomic DNA. Translation: AAX60127.1. AY905782 Genomic DNA. Translation: AAX60128.1. AY905783 Genomic DNA. Translation: AAX60129.1. AY905784 Genomic DNA. Translation: AAX60130.1. AY905785 Genomic DNA. Translation: AAX60131.1. AY905786 Genomic DNA. Translation: AAX60132.1. AY905787 Genomic DNA. Translation: AAX60133.1. AY905788 Genomic DNA. Translation: AAX60134.1. AY905789 Genomic DNA. Translation: AAX60135.1. AY905790 Genomic DNA. Translation: AAX60136.1. AY905791 Genomic DNA. Translation: AAX60137.1. AY905792 Genomic DNA. Translation: AAX60138.1. AY905793 Genomic DNA. Translation: AAX60139.1. AY905794 Genomic DNA. Translation: AAX60140.1. AY905795 Genomic DNA. Translation: AAX60141.1. AY905796 Genomic DNA. Translation: AAX60142.1. AY905797 Genomic DNA. Translation: AAX60143.1. AY905798 Genomic DNA. Translation: AAX60144.1. AY905799 Genomic DNA. Translation: AAX60145.1. AY905800 Genomic DNA. Translation: AAX60146.1. AY905801 Genomic DNA. Translation: AAX60147.1. AY905802 Genomic DNA. Translation: AAX60148.1. AY905803 Genomic DNA. Translation: AAX60149.1. AY905804 Genomic DNA. Translation: AAX60150.1. AY905805 Genomic DNA. Translation: AAX60151.1. AY905806 Genomic DNA. Translation: AAX60152.1. AY905807 Genomic DNA. Translation: AAX60153.1. AY905808 Genomic DNA. Translation: AAX60154.1. AY905809 Genomic DNA. Translation: AAX60155.1. AY905810 Genomic DNA. Translation: AAX60156.1. AY905811 Genomic DNA. Translation: AAX60157.1.
PIR	A46177.
RefSeq	NP_524503.2.
UniGene	Dm.2355
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:722N.
IntAct	Q01071. 17 interactions.
Proteomic databases
PRIDE	Q01071.
Genome annotation databases
Ensembl	FBgn0002734. Drosophila melanogaster. [Contig view]
GeneID	43150.
KEGG	dme:Dmel_CG8328.
Organism-specific databases
FlyBase	FBgn0002734. HLHmdelta.
Phylogenomic databases
HOGENOM	Q01071.
OMA	Q01071. AYEVSHI.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013495-MON.
Gene expression databases
ArrayExpress	Q01071.
GermOnline	CG8328. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR003650. Orange. IPR018352. Orange_subgroup. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	832427.

Entry information

Entry name

ESMD_DROME

Accession

Primary (citable) accession number: Q01071
Secondary accession number(s): Q01897 , Q4V723, Q56MT6, Q56MU1, Q56MU3, Q56MU4, Q56MU6, Q56MU8, Q56MV0, Q56MV5, Q56MV6, Q56MW2, Q56MW7, Q56MW9, Q56MX9, Q56MY0, Q56MY1, Q56N19, Q56N23, Q5S485, Q5S4A9, Q5S4K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project