Reviewed, UniProtKB/Swiss-Prot Q01070 (ESMC_DROME)

Names and origin

Protein names

Recommended name:
    Enhancer of split mgamma protein
      Short name=E(spl)mgamma
Alternative name(s):
    Split locus enhancer protein mB

Gene names

Name:	HLHmgamma
ORF Names:	CG8333

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional repressor of genes that require a bHLH protein for their transcription. May serve as a transcriptional regulator of the Achaete-scute complex (AS-C) genes. Contributes to the neural-epidermal lineage decision during early neurogenesis. Part of the Notch signaling pathway.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein (Groucho) By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in sensory organ precursors in the wing, leg and eye imaginal disk. Ref.3
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Notch signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW compound eye development Traceable author statement. Source: FlyBase negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: FlyBase nervous system development Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW wing disc dorsal/ventral pattern formation Inferred from genetic interaction. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Enhancer of split mgamma protein		PRO_0000127177
Regions
	Domain	29 – 73	45	Helix-loop-helix motif
	Domain	93 – 126	34	Orange
	DNA binding	16 – 28	13	Basic motif
	Motif	202 – 205	4	WRPW motif
Experimental info
	Sequence conflict	166	1	S → T in CAA47434. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01070-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4183A35D66DAB7E5	FASTA	205	23,135
	10 20 30 40 50 60 MSSLQMSEMS KTYQYRKVMK PMLERKRRAR INKCLDELKD LMVATLESEG EHVTRLEKAD 70 80 90 100 110 120 ILELTVTHLQ KMKQQRQHKR ASGDESLTPA EGFRSGYIHA VNEVSRSLSQ LPGMNVSLGT 130 140 150 160 170 180 QLMTHLGQRL NQIQPAEKEV LPVTAPLSVH IANRDAYSVP ISPISSYAGS PNSNTSSTSH 190 200 SLLTTIDVTK MEDDSEDEEN VWRPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Seven genes of the Enhancer of split complex of Drosophila melanogaster encode helix-loop-helix proteins." Knust E., Schrons H., Grawe F., Campos-Ortega J.A. Genetics 132:505-518(1992) [PubMed: 1427040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R. Tissue: Embryo.
[2]	"The Enhancer of split [E(spl)] locus of Drosophila encodes seven independent helix-loop-helix proteins." Delidakis C., Artavanis-Tsakonas S. Proc. Natl. Acad. Sci. U.S.A. 89:8731-8735(1992) [PubMed: 1528887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Embryo.
[3]	"Spatially restricted factors cooperate with Notch in the regulation of Enhancer of split genes." Cooper M.T.D., Tyler D.M., Furriols M., Chalkiadaki A., Delidakis C., Bray S. Dev. Biol. 221:390-403(2000) [PubMed: 10790334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[4]	"Identifying signatures of selection at the enhancer of split neurogenic gene complex in Drosophila." Macdonald S.J., Long A.D. Mol. Biol. Evol. 22:607-619(2005) [PubMed: 15537803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41, NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13, NVIII-m15 and NVIII-m19.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[7]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67049 mRNA. Translation: CAA47434.1. M96167 Genomic DNA. Translation: AAA28910.1. AJ276315 Genomic DNA. Translation: CAB77021.1. AY779906 Genomic DNA. Translation: AAV59046.1. AY779907 Genomic DNA. Translation: AAV59058.1. AY779908 Genomic DNA. Translation: AAV59070.1. AY779909 Genomic DNA. Translation: AAV59082.1. AY779910 Genomic DNA. Translation: AAV59094.1. AY779911 Genomic DNA. Translation: AAV59106.1. AY779912 Genomic DNA. Translation: AAV59118.1. AY779913 Genomic DNA. Translation: AAV59130.1. AY779914 Genomic DNA. Translation: AAV59142.1. AY779915 Genomic DNA. Translation: AAV59154.1. AY779916 Genomic DNA. Translation: AAV59166.1. AY779917 Genomic DNA. Translation: AAV59178.1. AY779918 Genomic DNA. Translation: AAV59190.1. AY779919 Genomic DNA. Translation: AAV59202.1. AY779920 Genomic DNA. Translation: AAV59214.1. AY779921 Genomic DNA. Translation: AAV59226.1. AE014297 Genomic DNA. Translation: AAF56545.1. BT022125 mRNA. Translation: AAY51520.1.
PIR	B46177.
RefSeq	NP_524504.2.
UniGene	Dm.1339
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:723N.
IntAct	Q01070. 13 interactions.
Proteomic databases
PRIDE	Q01070.
Genome annotation databases
Ensembl	FBgn0002735. Drosophila melanogaster. [Contig view]
GeneID	43151.
KEGG	dme:Dmel_CG8333.
NMPDR	fig|7227.3.peg.14760.
Organism-specific databases
FlyBase	FBgn0002735. HLHmgamma.
Phylogenomic databases
HOGENOM	Q01070.
OMA	Q01070. LESEGEH.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013496-MON.
Gene expression databases
GermOnline	CG8333. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR003650. Orange. IPR018352. Orange_subgroup. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	832432.

Entry information

Entry name

ESMC_DROME

Accession

Primary (citable) accession number: Q01070
Secondary accession number(s): Q01898, Q5S4K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project