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Q01066

- PDE1B_RAT

UniProt

Q01066 - PDE1B_RAT

Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

Pde1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate By similarity.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei222 – 2221Proton donorBy similarity
    Metal bindingi226 – 2261Divalent metal cation 1By similarity
    Metal bindingi262 – 2621Divalent metal cation 1By similarity
    Metal bindingi263 – 2631Divalent metal cation 1By similarity
    Metal bindingi263 – 2631Divalent metal cation 2By similarity
    Metal bindingi369 – 3691Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
    2. calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: RGD
    3. cyclic-nucleotide phosphodiesterase activity Source: RGD
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: RGD
    2. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
    3. cellular response to macrophage colony-stimulating factor stimulus Source: Ensembl
    4. cGMP catabolic process Source: RGD
    5. locomotory behavior Source: Ensembl
    6. monocyte differentiation Source: Ensembl
    7. regulation of dopamine metabolic process Source: Ensembl
    8. regulation of neurotransmitter levels Source: Ensembl
    9. response to amphetamine Source: Ensembl
    10. serotonin metabolic process Source: Ensembl
    11. signal transduction Source: Ensembl
    12. visual learning Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calmodulin-binding, cAMP, cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
    Short name:
    Cam-PDE 1B
    Alternative name(s):
    63 kDa Cam-PDE
    Gene namesi
    Name:Pde1b
    Synonyms:Pde1b1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi3278. Pde1b.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. neuronal cell body Source: RGD
    3. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BPRO_0000198791Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei465 – 4651PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ01066.
    PRIDEiQ01066.

    Expressioni

    Gene expression databases

    ArrayExpressiQ01066.
    GenevestigatoriQ01066.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01066.
    SMRiQ01066. Positions 145-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 4721Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni196 – 495300CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG139098.
    GeneTreeiENSGT00660000095451.
    HOGENOMiHOG000231888.
    HOVERGENiHBG056120.
    InParanoidiQ01066.
    KOiK13755.
    OMAiSTAVHNC.
    OrthoDBiEOG7X9G6J.
    PhylomeDBiQ01066.
    TreeFamiTF314638.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013706. PDEase_N.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    PF08499. PDEase_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01066-1 [UniParc]FASTAAdd to Basket

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    MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV    50
    NIEELKKNLE YTASLLEAVY IDETRQILDT EDELRELRSD AVPSEVRDWL 100
    ASTFTQQTRA KGRRAEEKPK FRSIVHAVQA GIFVERMFRR TYTAVGPTYS 150
    TAVHNCLKNL DVWCFDVFSL NRAADDHALR TIVFELLTRH SLISRFKIPT 200
    VFLMSFLEAL ETGYGKYKNP YHNQIHAADV TQTVHCFLLR TGMVHCLSEI 250
    EVLAIIFAAA IHDYEHTGTT NSFHIQTKSE CAILYNDRSV LENHHISSVF 300
    RMMQDDEMNI FINLTKDEFV ELRALVIEMV LATDMSCHFQ QVKTMKTALQ 350
    QLERIDKSKA LSLLLHAADI SHPTKQWSVH SRWTKALMEE FFRQGDKEAE 400
    LGLPFSPLCD RTSTLVAQSQ IGFIDFIVEP TFSVLTDVAE KSVQPLTDDD 450
    SKSKSQPSFQ WRQPSLDVDV GDPNPDVVSF RSTWTKYIQE NKQKWKERAA 500
    SGITNQMSID ELSPCEEEAP SSPAEDEHNQ NGNLD 535
    Length:535
    Mass (Da):61,260
    Last modified:April 1, 1993 - v1
    Checksum:iF98FFFE61F848F89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94537 mRNA. Translation: AAA16530.1.
    AF327906 mRNA. Translation: AAK15740.1.
    PIRiA44161.
    RefSeqiNP_073201.1. NM_022710.1.
    UniGeneiRn.53489.

    Genome annotation databases

    EnsembliENSRNOT00000055272; ENSRNOP00000052147; ENSRNOG00000036828.
    GeneIDi29691.
    KEGGirno:29691.
    UCSCiRGD:3278. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94537 mRNA. Translation: AAA16530.1 .
    AF327906 mRNA. Translation: AAK15740.1 .
    PIRi A44161.
    RefSeqi NP_073201.1. NM_022710.1.
    UniGenei Rn.53489.

    3D structure databases

    ProteinModelPortali Q01066.
    SMRi Q01066. Positions 145-501.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q01066.
    ChEMBLi CHEMBL2111322.

    Proteomic databases

    PaxDbi Q01066.
    PRIDEi Q01066.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000055272 ; ENSRNOP00000052147 ; ENSRNOG00000036828 .
    GeneIDi 29691.
    KEGGi rno:29691.
    UCSCi RGD:3278. rat.

    Organism-specific databases

    CTDi 5153.
    RGDi 3278. Pde1b.

    Phylogenomic databases

    eggNOGi NOG139098.
    GeneTreei ENSGT00660000095451.
    HOGENOMi HOG000231888.
    HOVERGENi HBG056120.
    InParanoidi Q01066.
    KOi K13755.
    OMAi STAVHNC.
    OrthoDBi EOG7X9G6J.
    PhylomeDBi Q01066.
    TreeFami TF314638.

    Miscellaneous databases

    NextBioi 610076.
    PROi Q01066.

    Gene expression databases

    ArrayExpressi Q01066.
    Genevestigatori Q01066.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013706. PDEase_N.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    PF08499. PDEase_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A polymerase chain reaction strategy to identify and clone cyclic nucleotide phosphodiesterase cDNAs. Molecular cloning of the cDNA encoding the 63-kDa calmodulin-dependent phosphodiesterase."
      Repaske D.R., Swinnen J.V., Jin S.-L.C., van Wyk J.J., Conti M.
      J. Biol. Chem. 267:18683-18688(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Phosphodiesterase 1B (PDE1B) in rat brain."
      Prime G.R., Sutor B.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.

    Entry informationi

    Entry nameiPDE1B_RAT
    AccessioniPrimary (citable) accession number: Q01066
    Secondary accession number(s): Q548L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3