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Q01065

- PDE1B_MOUSE

UniProt

Q01065 - PDE1B_MOUSE

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Protein
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
Gene
Pde1b, Pde1b1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate By similarity.

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei222 – 2221Proton donor By similarity
Metal bindingi226 – 2261Divalent metal cation 1 By similarity
Metal bindingi262 – 2621Divalent metal cation 1 By similarity
Metal bindingi263 – 2631Divalent metal cation 1 By similarity
Metal bindingi263 – 2631Divalent metal cation 2 By similarity
Metal bindingi369 – 3691Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: Ensembl
  2. calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
  3. calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
  4. cyclic-nucleotide phosphodiesterase activity Source: MGI
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: Ensembl
  2. cGMP catabolic process Source: Ensembl
  3. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
  4. cellular response to macrophage colony-stimulating factor stimulus Source: Ensembl
  5. locomotory behavior Source: MGI
  6. monocyte differentiation Source: Ensembl
  7. regulation of dopamine metabolic process Source: MGI
  8. regulation of neurotransmitter levels Source: MGI
  9. response to amphetamine Source: MGI
  10. serotonin metabolic process Source: MGI
  11. signal transduction Source: MGI
  12. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calmodulin-binding, cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_227001. Cam-PDE 1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:Pde1b
Synonyms:Pde1b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:97523. Pde1b.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. neuronal cell body Source: Ensembl
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
PRO_0000198790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei465 – 4651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01065.
PaxDbiQ01065.
PRIDEiQ01065.

PTM databases

PhosphoSiteiQ01065.

Expressioni

Gene expression databases

ArrayExpressiQ01065.
BgeeiQ01065.
CleanExiMM_PDE1B.
GenevestigatoriQ01065.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi202075. 2 interactions.
IntActiQ01065. 1 interaction.
MINTiMINT-4106830.

Structurei

3D structure databases

ProteinModelPortaliQ01065.
SMRiQ01065. Positions 145-501.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 4721Calmodulin-binding Reviewed prediction
Add
BLAST
Regioni196 – 495300Catalytic By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG139098.
GeneTreeiENSGT00660000095451.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiQ01065.
KOiK13755.
OMAiSTAVHNC.
OrthoDBiEOG7X9G6J.
PhylomeDBiQ01065.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01065-1 [UniParc]FASTAAdd to Basket

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MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV    50
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELRELRSD AVPSEVRDWL 100
ASTFTQQTRA KGRRAEEKPK FRSIVHAVQA GIFVERMFRR TYTSVGPTYS 150
TAVHNCLKNL DLWCFDVFSL NRAADDHALR TIVFELLTRH SLISRFKIPT 200
VFLMSFLEAL ETGYGKYKNP YHNQIHAADV TQTVHCFLLR TGMVHCLSEI 250
EVLAIIFAAA IHDYEHTGTT NSFHIQTKSE CAILYNDRSV LENHHISSVF 300
RMMQDDEMNI FINLTKDEFA ELRALVIEMV LATDMSCHFQ QVKTMKTALQ 350
QLERIDKSKA LSLLLHAADI SHPTKQWSVH SRWTKALMEE FFRQGDKEAE 400
LGLPFSPLCD RTSTLVAQSQ IGFIDFIVEP TFSVLTDVAE KSVQPLADDD 450
SKPKSQPSFQ WRQPSLDVDV GDPNPDVVSF RATWTKYIQE NKQKWKERAA 500
SGITNQMSID ELSPCEEEAP SSPAEDEHNQ NGNLD 535
Length:535
Mass (Da):61,226
Last modified:October 1, 1996 - v2
Checksum:iF87A585537C8D1CD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381L → M1 Publication
Sequence conflicti224 – 2241Q → R in AAA37367. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01695 mRNA. Translation: AAA39902.1.
AH007066 Genomic DNA. Translation: AAC96022.1.
M94538 mRNA. Translation: AAA37367.1.
CCDSiCCDS27904.1.
PIRiA46378.
RefSeqiNP_032826.1. NM_008800.2.
UniGeneiMm.390792.

Genome annotation databases

EnsembliENSMUST00000023132; ENSMUSP00000023132; ENSMUSG00000022489.
GeneIDi18574.
KEGGimmu:18574.
UCSCiuc007xyh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01695 mRNA. Translation: AAA39902.1 .
AH007066 Genomic DNA. Translation: AAC96022.1 .
M94538 mRNA. Translation: AAA37367.1 .
CCDSi CCDS27904.1.
PIRi A46378.
RefSeqi NP_032826.1. NM_008800.2.
UniGenei Mm.390792.

3D structure databases

ProteinModelPortali Q01065.
SMRi Q01065. Positions 145-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202075. 2 interactions.
IntActi Q01065. 1 interaction.
MINTi MINT-4106830.

PTM databases

PhosphoSitei Q01065.

Proteomic databases

MaxQBi Q01065.
PaxDbi Q01065.
PRIDEi Q01065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023132 ; ENSMUSP00000023132 ; ENSMUSG00000022489 .
GeneIDi 18574.
KEGGi mmu:18574.
UCSCi uc007xyh.1. mouse.

Organism-specific databases

CTDi 5153.
MGIi MGI:97523. Pde1b.

Phylogenomic databases

eggNOGi NOG139098.
GeneTreei ENSGT00660000095451.
HOGENOMi HOG000231888.
HOVERGENi HBG056120.
InParanoidi Q01065.
KOi K13755.
OMAi STAVHNC.
OrthoDBi EOG7X9G6J.
PhylomeDBi Q01065.
TreeFami TF314638.

Enzyme and pathway databases

Reactomei REACT_227001. Cam-PDE 1 activation.

Miscellaneous databases

NextBioi 294424.
PROi Q01065.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q01065.
Bgeei Q01065.
CleanExi MM_PDE1B.
Genevestigatori Q01065.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of DNA encoding a calmodulin-dependent phosphodiesterase enriched in striatum."
    Polli J.W., Kincaid R.L.
    Proc. Natl. Acad. Sci. U.S.A. 89:11079-11083(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure and chromosome location of the murine PDE1B phosphodiesterase gene."
    Reed T.M., Browning J.E., Blough R.I., Vorhees C.V., Repaske D.R.
    Mamm. Genome 9:571-576(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-535.
    Strain: 129/SvJ.
  3. "A polymerase chain reaction strategy to identify and clone cyclic nucleotide phosphodiesterase cDNAs. Molecular cloning of the cDNA encoding the 63-kDa calmodulin-dependent phosphodiesterase."
    Repaske D.R., Swinnen J.V., Jin S.-L.C., van Wyk J.J., Conti M.
    J. Biol. Chem. 267:18683-18688(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 221-336.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiPDE1B_MOUSE
AccessioniPrimary (citable) accession number: Q01065
Secondary accession number(s): O35384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi