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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

Pde1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei222Proton donorBy similarity1
Metal bindingi226Divalent metal cation 1By similarity1
Metal bindingi262Divalent metal cation 1By similarity1
Metal bindingi263Divalent metal cation 1By similarity1
Metal bindingi263Divalent metal cation 2By similarity1
Metal bindingi369Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • cAMP catabolic process Source: MGI
  • cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: MGI
  • cellular response to macrophage colony-stimulating factor stimulus Source: MGI
  • cGMP catabolic process Source: MGI
  • locomotory behavior Source: MGI
  • monocyte differentiation Source: Ensembl
  • regulation of dopamine metabolic process Source: MGI
  • regulation of neurotransmitter levels Source: MGI
  • response to amphetamine Source: MGI
  • serotonin metabolic process Source: MGI
  • signal transduction Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calmodulin-binding, cAMP, cGMP, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.17. 3474.
ReactomeiR-MMU-111957. Cam-PDE 1 activation.
R-MMU-418457. cGMP effects.
R-MMU-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:Pde1b
Synonyms:Pde1b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97523. Pde1b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987901 – 535Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BAdd BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01065.
PaxDbiQ01065.
PeptideAtlasiQ01065.
PRIDEiQ01065.

PTM databases

iPTMnetiQ01065.
PhosphoSitePlusiQ01065.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022489.
CleanExiMM_PDE1B.
GenevisibleiQ01065. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi202075. 2 interactors.
IntActiQ01065. 1 interactor.
MINTiMINT-4106830.
STRINGi10090.ENSMUSP00000023132.

Structurei

3D structure databases

ProteinModelPortaliQ01065.
SMRiQ01065.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 47Calmodulin-bindingSequence analysisAdd BLAST21
Regioni196 – 495CatalyticBy similarityAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688. Eukaryota.
ENOG410XQDD. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiQ01065.
KOiK13755.
OMAiLMQDDEM.
OrthoDBiEOG091G04C4.
PhylomeDBiQ01065.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV
60 70 80 90 100
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELRELRSD AVPSEVRDWL
110 120 130 140 150
ASTFTQQTRA KGRRAEEKPK FRSIVHAVQA GIFVERMFRR TYTSVGPTYS
160 170 180 190 200
TAVHNCLKNL DLWCFDVFSL NRAADDHALR TIVFELLTRH SLISRFKIPT
210 220 230 240 250
VFLMSFLEAL ETGYGKYKNP YHNQIHAADV TQTVHCFLLR TGMVHCLSEI
260 270 280 290 300
EVLAIIFAAA IHDYEHTGTT NSFHIQTKSE CAILYNDRSV LENHHISSVF
310 320 330 340 350
RMMQDDEMNI FINLTKDEFA ELRALVIEMV LATDMSCHFQ QVKTMKTALQ
360 370 380 390 400
QLERIDKSKA LSLLLHAADI SHPTKQWSVH SRWTKALMEE FFRQGDKEAE
410 420 430 440 450
LGLPFSPLCD RTSTLVAQSQ IGFIDFIVEP TFSVLTDVAE KSVQPLADDD
460 470 480 490 500
SKPKSQPSFQ WRQPSLDVDV GDPNPDVVSF RATWTKYIQE NKQKWKERAA
510 520 530
SGITNQMSID ELSPCEEEAP SSPAEDEHNQ NGNLD
Length:535
Mass (Da):61,226
Last modified:October 1, 1996 - v2
Checksum:iF87A585537C8D1CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38L → M (PubMed:9657856).Curated1
Sequence conflicti224Q → R in AAA37367 (PubMed:1326532).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01695 mRNA. Translation: AAA39902.1.
AH007066 Genomic DNA. Translation: AAC96022.1.
M94538 mRNA. Translation: AAA37367.1.
CCDSiCCDS27904.1.
PIRiA46378.
RefSeqiNP_032826.1. NM_008800.2.
UniGeneiMm.390792.

Genome annotation databases

EnsembliENSMUST00000023132; ENSMUSP00000023132; ENSMUSG00000022489.
GeneIDi18574.
KEGGimmu:18574.
UCSCiuc007xyh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01695 mRNA. Translation: AAA39902.1.
AH007066 Genomic DNA. Translation: AAC96022.1.
M94538 mRNA. Translation: AAA37367.1.
CCDSiCCDS27904.1.
PIRiA46378.
RefSeqiNP_032826.1. NM_008800.2.
UniGeneiMm.390792.

3D structure databases

ProteinModelPortaliQ01065.
SMRiQ01065.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202075. 2 interactors.
IntActiQ01065. 1 interactor.
MINTiMINT-4106830.
STRINGi10090.ENSMUSP00000023132.

PTM databases

iPTMnetiQ01065.
PhosphoSitePlusiQ01065.

Proteomic databases

MaxQBiQ01065.
PaxDbiQ01065.
PeptideAtlasiQ01065.
PRIDEiQ01065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023132; ENSMUSP00000023132; ENSMUSG00000022489.
GeneIDi18574.
KEGGimmu:18574.
UCSCiuc007xyh.2. mouse.

Organism-specific databases

CTDi5153.
MGIiMGI:97523. Pde1b.

Phylogenomic databases

eggNOGiKOG3688. Eukaryota.
ENOG410XQDD. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiQ01065.
KOiK13755.
OMAiLMQDDEM.
OrthoDBiEOG091G04C4.
PhylomeDBiQ01065.
TreeFamiTF314638.

Enzyme and pathway databases

BRENDAi3.1.4.17. 3474.
ReactomeiR-MMU-111957. Cam-PDE 1 activation.
R-MMU-418457. cGMP effects.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

PROiQ01065.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022489.
CleanExiMM_PDE1B.
GenevisibleiQ01065. MM.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDE1B_MOUSE
AccessioniPrimary (citable) accession number: Q01065
Secondary accession number(s): O35384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.