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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

Pde1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei222Proton donorBy similarity1
Metal bindingi226Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi262Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi263Divalent metal cation 1By similarity1
Metal bindingi263Divalent metal cation 2By similarity1
Metal bindingi369Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • cAMP catabolic process Source: MGI
  • cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: MGI
  • cellular response to macrophage colony-stimulating factor stimulus Source: MGI
  • cGMP catabolic process Source: MGI
  • locomotory behavior Source: MGI
  • monocyte differentiation Source: Ensembl
  • regulation of dopamine metabolic process Source: MGI
  • regulation of neurotransmitter levels Source: MGI
  • response to amphetamine Source: MGI
  • serotonin metabolic process Source: MGI
  • signal transduction Source: MGI
  • visual learning Source: MGI

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.17 3474
ReactomeiR-MMU-111957 Cam-PDE 1 activation
R-MMU-418457 cGMP effects
R-MMU-418555 G alpha (s) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:Pde1b
Synonyms:Pde1b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97523 Pde1b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987901 – 535Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BAdd BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01065
PaxDbiQ01065
PeptideAtlasiQ01065
PRIDEiQ01065

PTM databases

iPTMnetiQ01065
PhosphoSitePlusiQ01065

Expressioni

Gene expression databases

BgeeiENSMUSG00000022489
CleanExiMM_PDE1B
ExpressionAtlasiQ01065 baseline and differential
GenevisibleiQ01065 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202075, 3 interactors
IntActiQ01065, 2 interactors
MINTiQ01065
STRINGi10090.ENSMUSP00000023132

Structurei

3D structure databases

ProteinModelPortaliQ01065
SMRiQ01065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 502PDEasePROSITE-ProRule annotationAdd BLAST358

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 47Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688 Eukaryota
ENOG410XQDD LUCA
GeneTreeiENSGT00760000118889
HOGENOMiHOG000231888
HOVERGENiHBG056120
InParanoidiQ01065
KOiK13755
OMAiLMQDDEM
OrthoDBiEOG091G04C4
PhylomeDBiQ01065
TreeFamiTF314638

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
IPR013706 PDEase_N
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
PF08499 PDEase_I_N, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q01065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV
60 70 80 90 100
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELRELRSD AVPSEVRDWL
110 120 130 140 150
ASTFTQQTRA KGRRAEEKPK FRSIVHAVQA GIFVERMFRR TYTSVGPTYS
160 170 180 190 200
TAVHNCLKNL DLWCFDVFSL NRAADDHALR TIVFELLTRH SLISRFKIPT
210 220 230 240 250
VFLMSFLEAL ETGYGKYKNP YHNQIHAADV TQTVHCFLLR TGMVHCLSEI
260 270 280 290 300
EVLAIIFAAA IHDYEHTGTT NSFHIQTKSE CAILYNDRSV LENHHISSVF
310 320 330 340 350
RMMQDDEMNI FINLTKDEFA ELRALVIEMV LATDMSCHFQ QVKTMKTALQ
360 370 380 390 400
QLERIDKSKA LSLLLHAADI SHPTKQWSVH SRWTKALMEE FFRQGDKEAE
410 420 430 440 450
LGLPFSPLCD RTSTLVAQSQ IGFIDFIVEP TFSVLTDVAE KSVQPLADDD
460 470 480 490 500
SKPKSQPSFQ WRQPSLDVDV GDPNPDVVSF RATWTKYIQE NKQKWKERAA
510 520 530
SGITNQMSID ELSPCEEEAP SSPAEDEHNQ NGNLD
Length:535
Mass (Da):61,226
Last modified:October 1, 1996 - v2
Checksum:iF87A585537C8D1CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38L → M (PubMed:9657856).Curated1
Sequence conflicti224Q → R in AAA37367 (PubMed:1326532).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01695 mRNA Translation: AAA39902.1
AH007066 Genomic DNA Translation: AAC96022.1
M94538 mRNA Translation: AAA37367.1
CCDSiCCDS27904.1
PIRiA46378
RefSeqiNP_032826.1, NM_008800.2
UniGeneiMm.390792

Genome annotation databases

EnsembliENSMUST00000023132; ENSMUSP00000023132; ENSMUSG00000022489
GeneIDi18574
KEGGimmu:18574
UCSCiuc007xyh.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPDE1B_MOUSE
AccessioniPrimary (citable) accession number: Q01065
Secondary accession number(s): O35384
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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