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Q01062

- PDE2A_RAT

UniProt

Q01062 - PDE2A_RAT

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Protein

cGMP-dependent 3',5'-cyclic phosphodiesterase

Gene

Pde2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei419 – 4191cGMPBy similarity
Binding sitei434 – 4341cGMPBy similarity
Binding sitei487 – 4871cGMPBy similarity
Active sitei644 – 6441Proton donorBy similarity
Metal bindingi648 – 6481Divalent metal cation 1By similarity
Metal bindingi684 – 6841Divalent metal cation 1By similarity
Metal bindingi685 – 6851Divalent metal cation 1By similarity
Metal bindingi685 – 6851Divalent metal cation 2By similarity
Binding sitei685 – 6851SubstrateBy similarity
Metal bindingi796 – 7961Divalent metal cation 1By similarity
Binding sitei796 – 7961SubstrateBy similarity

GO - Molecular functioni

  1. cAMP binding Source: RGD
  2. cGMP binding Source: RGD
  3. cGMP-stimulated cyclic-nucleotide phosphodiesterase activity Source: RGD
  4. cyclic-nucleotide phosphodiesterase activity Source: UniProtKB
  5. drug binding Source: UniProtKB
  6. metal ion binding Source: UniProtKB-KW
  7. protein homodimerization activity Source: RGD

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB
  2. cAMP-mediated signaling Source: UniProtKB
  3. cellular response to cGMP Source: UniProtKB
  4. cellular response to drug Source: UniProtKB
  5. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: UniProtKB
  6. cellular response to macrophage colony-stimulating factor stimulus Source: UniProtKB
  7. cellular response to mechanical stimulus Source: UniProtKB
  8. cGMP catabolic process Source: RGD
  9. cGMP-mediated signaling Source: UniProtKB
  10. establishment of endothelial barrier Source: UniProtKB
  11. negative regulation of cAMP biosynthetic process Source: UniProtKB
  12. negative regulation of protein import into nucleus, translocation Source: UniProtKB
  13. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  14. negative regulation of vascular permeability Source: UniProtKB
  15. positive regulation of inflammatory response Source: UniProtKB
  16. positive regulation of vascular permeability Source: UniProtKB
  17. protein targeting to mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ01062.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent 3',5'-cyclic phosphodiesterase (EC:3.1.4.17)
Alternative name(s):
Cyclic GMP-stimulated phosphodiesterase
Short name:
CGS-PDE
Short name:
cGSPDE
Gene namesi
Name:Pde2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620965. Pde2a.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB
  4. Golgi apparatus Source: UniProtKB
  5. membrane raft Source: RGD
  6. mitochondrial matrix Source: UniProtKB
  7. nucleus Source: UniProtKB
  8. perinuclear region of cytoplasm Source: UniProtKB
  9. plasma membrane Source: UniProtKB
  10. presynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928cGMP-dependent 3',5'-cyclic phosphodiesterasePRO_0000198798Add
BLAST

Proteomic databases

PRIDEiQ01062.

PTM databases

PhosphoSiteiQ01062.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

GenevestigatoriQ01062.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AipQ5FWY52EBI-1786062,EBI-1786045

Protein-protein interaction databases

IntActiQ01062. 1 interaction.
STRINGi10116.ENSRNOP00000026586.

Structurei

3D structure databases

ProteinModelPortaliQ01062.
SMRiQ01062. Positions 210-550, 566-903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini228 – 365138GAF 1Add
BLAST
Domaini397 – 536140GAF 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni621 – 879259CatalyticBy similarityAdd
BLAST
Regioni644 – 6485Substrate bindingBy similarity

Domaini

GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme.By similarity

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
HOGENOMiHOG000007068.
HOVERGENiHBG053540.
InParanoidiQ01062.
KOiK18283.
PhylomeDBiQ01062.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 3 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform PDE2A3 (identifier: Q01062-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLVLHHILI AVVQFLRRGQ QVFLKPDEPP PQPCADSLQD ALLSLGAVID
60 70 80 90 100
IAGLRQAAKD ALSAVLPKVE TVYTYLVDGE SRLVCEDPPH ELPQEGKIRE
110 120 130 140 150
AVISRKRLSC DGLGPSDLLG KPLARLVAPL APDTQVLVIP LLDKETGTVA
160 170 180 190 200
AVILVHCGQL SDSEEQSLQV VEKHALVALQ RVQALQQRRP EAVQNTSADP
210 220 230 240 250
SEDQKDEKGY TAHDRKILQL CGELYDLDAT SLQLKVLRYL QQETQATHCC
260 270 280 290 300
LLLVSEDNLQ LSCKVIGEKV LGEEVSFPLT MGRLGQVVED KQCIQLKDLT
310 320 330 340 350
SDDVQQLQNM LGCELRAMLC VPVISRATDQ VVALACAFNK LGGDFFTDED
360 370 380 390 400
ERAIQHCFHY TGTVLTSTLA FQKEQKLKCE CQALLQVAKN LFTHLDDVSV
410 420 430 440 450
LLQEIITEAR NLSNAEICSV FLLDQNELVA KVFDGGVVDD ESYEIRIPAD
460 470 480 490 500
QGIAGHVATT GQILNIPDAY AHPLFYRGVD DSTGFRTRNI LCFPIKNENQ
510 520 530 540 550
EVIGVAELVN KINGPWFSKF DEDLATAFSI YCGISIAHSL LYKKVNEAQY
560 570 580 590 600
RSHLANEMMM YHMKVSDDEY TKLLHDGIQP VAAIDSNFAN FTYTPRSLPE
610 620 630 640 650
DDTSMAILSM LQDMNFINNY KIDCPTLARF CLMVKKGYRD PPYHNWMHAF
660 670 680 690 700
SVSHFCYLLY KNLELSNYLE DIEIFALFIS CMCHDLDHRG TNNSFQVASK
710 720 730 740 750
SVLAALYSSE GSVMERHHFA QAIAILNTHG CNIFDHFSRK DYQRMLDLMR
760 770 780 790 800
DIILATDLAH HLRIFKDLQK MAEVGYDRNN KQHHRLLLCL LMTSCDLSDQ
810 820 830 840 850
TKGWKTTRKI AELIYKEFFS QGDLEKAMGN RPMEMMDREK AYIPELQISF
860 870 880 890 900
MEHIAMPIYK LLQDLFPKAA ELYERVASNR EHWTKVSHKF TIRGLPSNNS
910 920
LDFLDEEYEV PDLDVTRAPV NGCCSLEG
Length:928
Mass (Da):104,664
Last modified:October 1, 1996 - v2
Checksum:iEF0B8C1E266EAB18
GO
Isoform PDE2A1 (identifier: Q01062-2)

Sequence is not available
Length:
Mass (Da):
Isoform PDE2A2 (identifier: Q01062-3)

Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti646 – 6461W → R in AAA40922. (PubMed:1326532)Curated
Sequence conflicti758 – 7581L → M(PubMed:1326532)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21101 mRNA. Translation: AAA63683.1.
M94540 mRNA. Translation: AAA40922.1.
PIRiJC2486.
RefSeqiNP_001137319.1. NM_001143847.1.
NP_112341.1. NM_031079.1. [Q01062-1]
UniGeneiRn.10044.

Genome annotation databases

GeneIDi81743.
KEGGirno:81743.
UCSCiRGD:620965. rat. [Q01062-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21101 mRNA. Translation: AAA63683.1 .
M94540 mRNA. Translation: AAA40922.1 .
PIRi JC2486.
RefSeqi NP_001137319.1. NM_001143847.1.
NP_112341.1. NM_031079.1. [Q01062-1 ]
UniGenei Rn.10044.

3D structure databases

ProteinModelPortali Q01062.
SMRi Q01062. Positions 210-550, 566-903.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q01062. 1 interaction.
STRINGi 10116.ENSRNOP00000026586.

Chemistry

BindingDBi Q01062.
ChEMBLi CHEMBL4650.

PTM databases

PhosphoSitei Q01062.

Proteomic databases

PRIDEi Q01062.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81743.
KEGGi rno:81743.
UCSCi RGD:620965. rat. [Q01062-1 ]

Organism-specific databases

CTDi 5138.
RGDi 620965. Pde2a.

Phylogenomic databases

eggNOGi NOG270709.
HOGENOMi HOG000007068.
HOVERGENi HBG053540.
InParanoidi Q01062.
KOi K18283.
PhylomeDBi Q01062.

Enzyme and pathway databases

SABIO-RK Q01062.

Miscellaneous databases

NextBioi 615464.
PROi Q01062.

Gene expression databases

Genevestigatori Q01062.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 3 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "A polymerase chain reaction strategy to identify and clone cyclic nucleotide phosphodiesterase cDNAs. Molecular cloning of the cDNA encoding the 63-kDa calmodulin-dependent phosphodiesterase."
    Repaske D.R., Swinnen J.V., Jin S.-L.C., van Wyk J.J., Conti M.
    J. Biol. Chem. 267:18683-18688(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 643-759.

Entry informationi

Entry nameiPDE2A_RAT
AccessioniPrimary (citable) accession number: Q01062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

cGMP binds at an allosteric activator site.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3