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Q01061

- PDE1B_BOVIN

UniProt

Q01061 - PDE1B_BOVIN

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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

PDE1B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+. Regulated by phosphorylation by CaM-dependent protein kinase II.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Proton donorBy similarity
Metal bindingi225 – 2251Divalent metal cation 1By similarity
Metal bindingi261 – 2611Divalent metal cation 1By similarity
Metal bindingi262 – 2621Divalent metal cation 1By similarity
Metal bindingi262 – 2621Divalent metal cation 2By similarity
Metal bindingi368 – 3681Divalent metal cation 1By similarity

GO - Molecular functioni

  1. calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
  2. calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. metabolic process Source: GOC
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calmodulin-binding, cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_205403. cGMP effects.
REACT_206547. G alpha (s) signalling events.
REACT_212942. Cam-PDE 1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:PDE1B
Synonyms:PDE1B1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BPRO_0000198787Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei464 – 4641PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ01061.

Expressioni

Tissue specificityi

Expressed in central nervous system regions. Most abundant in basal ganglia. Also found in kidney papilla and adrenal medulla.

Gene expression databases

ExpressionAtlasiQ01061. baseline.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi159261. 2 interactions.
STRINGi9913.ENSBTAP00000005680.

Structurei

3D structure databases

ProteinModelPortaliQ01061.
SMRiQ01061. Positions 144-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 4721Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni195 – 494300CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG139098.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiQ01061.
KOiK13755.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01061-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV
60 70 80 90 100
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELQELRSD AVPSEVRDWL
110 120 130 140 150
ASTFTQQTRA KGPSEEKPKF RSIVHAVQAG IFVERMFRRT YTSVGPTYST
160 170 180 190 200
AVLNCLKNVD LWCFDVFSLN RAADDHALRT IVFELLTRHN LISRFKIPTV
210 220 230 240 250
FLMTFLDALE TGYGKYKNPY HNQIHAADVT QTVHCFLLRT GMVHCLSEIE
260 270 280 290 300
VLAIIFAAAI HDYEHTGTTN SFHIQTKSEC AILYNDRSVL ENHHISSVFR
310 320 330 340 350
MMQDDEMNIF INLTKDEFVE LRALVIEMVL ATDMSCHFQQ VKSMKTALQQ
360 370 380 390 400
LERIDKSKAL SLLLHAADIS HPTKQWSVHS RWTKALMEEF FRQGDKEAEL
410 420 430 440 450
GLPFSPLCDR TSTLVAQSQI GFIDFIVEPT FSVLTDVAEK SVQPTGDDDS
460 470 480 490 500
KSKNQPSFQW RQPSLDVEVG DPNPDVVSFR STWTKYIQEN KQKWKERAAS
510 520 530
GITNQMSIDE LSPCEEEAPA SPAEDEHNQN GNLD
Length:534
Mass (Da):61,006
Last modified:June 1, 1994 - v1
Checksum:iC86C3F48E0AE9B69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94867 mRNA. Translation: AAA74558.1.
PIRiA44162.
RefSeqiNP_776840.1. NM_174415.2.
UniGeneiBt.3910.

Genome annotation databases

EnsembliENSBTAT00000003552; ENSBTAP00000003552; ENSBTAG00000004337.
GeneIDi281970.
KEGGibta:281970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94867 mRNA. Translation: AAA74558.1 .
PIRi A44162.
RefSeqi NP_776840.1. NM_174415.2.
UniGenei Bt.3910.

3D structure databases

ProteinModelPortali Q01061.
SMRi Q01061. Positions 144-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 159261. 2 interactions.
STRINGi 9913.ENSBTAP00000005680.

Chemistry

ChEMBLi CHEMBL2650.

Proteomic databases

PRIDEi Q01061.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000003552 ; ENSBTAP00000003552 ; ENSBTAG00000004337 .
GeneIDi 281970.
KEGGi bta:281970.

Organism-specific databases

CTDi 5153.

Phylogenomic databases

eggNOGi NOG139098.
GeneTreei ENSGT00760000118889.
HOGENOMi HOG000231888.
HOVERGENi HBG056120.
InParanoidi Q01061.
KOi K13755.

Enzyme and pathway databases

Reactomei REACT_205403. cGMP effects.
REACT_206547. G alpha (s) signalling events.
REACT_212942. Cam-PDE 1 activation.

Miscellaneous databases

NextBioi 20805841.

Gene expression databases

ExpressionAtlasi Q01061. baseline.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of cDNA encoding a '63'-kDa calmodulin-stimulated phosphodiesterase from bovine brain."
    Bentley J.K., Kadlecek A., Sherbert C.H., Seger D., Sonnenburg W.K., Charbonneau H., Novack J.P., Beavo J.A.
    J. Biol. Chem. 267:18676-18682(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Sequence comparison of the 63-, 61-, and 59-kDa calmodulin-dependent cyclic nucleotide phosphodiesterases."
    Novack J.P., Charbonneau H., Bentley J.K., Walsh K.A., Beavo J.A.
    Biochemistry 30:7940-7947(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.

Entry informationi

Entry nameiPDE1B_BOVIN
AccessioniPrimary (citable) accession number: Q01061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3