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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

PDE1B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+. Regulated by phosphorylation by CaM-dependent protein kinase II.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei221Proton donorBy similarity1
Metal bindingi225Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi261Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi262Divalent metal cation 1By similarity1
Metal bindingi262Divalent metal cation 2By similarity1
Metal bindingi368Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-111957. Cam-PDE 1 activation.
R-BTA-418457. cGMP effects.
R-BTA-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:PDE1B
Synonyms:PDE1B1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2650.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987871 – 534Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BAdd BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei512PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01061.
PRIDEiQ01061.

Expressioni

Tissue specificityi

Expressed in central nervous system regions. Most abundant in basal ganglia. Also found in kidney papilla and adrenal medulla.

Gene expression databases

BgeeiENSBTAG00000004337.
ExpressionAtlasiQ01061. baseline.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159261. 2 interactors.
STRINGi9913.ENSBTAP00000005680.

Chemistry databases

BindingDBiQ01061.

Structurei

3D structure databases

ProteinModelPortaliQ01061.
SMRiQ01061.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 47Calmodulin-bindingSequence analysisAdd BLAST21
Regioni195 – 494CatalyticBy similarityAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688. Eukaryota.
ENOG410XQDD. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiQ01061.
KOiK13755.

Family and domain databases

CDDicd00077. HDc. 1 hit.
Gene3Di1.10.1300.10. 1 hit.
InterProiView protein in InterPro
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR036971. PDEase_catalytic_dom_sf.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
PfamiView protein in Pfam
PF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
PRINTSiPR00387. PDIESTERASE1.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
PROSITEiView protein in PROSITE
PS00126. PDEASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV
60 70 80 90 100
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELQELRSD AVPSEVRDWL
110 120 130 140 150
ASTFTQQTRA KGPSEEKPKF RSIVHAVQAG IFVERMFRRT YTSVGPTYST
160 170 180 190 200
AVLNCLKNVD LWCFDVFSLN RAADDHALRT IVFELLTRHN LISRFKIPTV
210 220 230 240 250
FLMTFLDALE TGYGKYKNPY HNQIHAADVT QTVHCFLLRT GMVHCLSEIE
260 270 280 290 300
VLAIIFAAAI HDYEHTGTTN SFHIQTKSEC AILYNDRSVL ENHHISSVFR
310 320 330 340 350
MMQDDEMNIF INLTKDEFVE LRALVIEMVL ATDMSCHFQQ VKSMKTALQQ
360 370 380 390 400
LERIDKSKAL SLLLHAADIS HPTKQWSVHS RWTKALMEEF FRQGDKEAEL
410 420 430 440 450
GLPFSPLCDR TSTLVAQSQI GFIDFIVEPT FSVLTDVAEK SVQPTGDDDS
460 470 480 490 500
KSKNQPSFQW RQPSLDVEVG DPNPDVVSFR STWTKYIQEN KQKWKERAAS
510 520 530
GITNQMSIDE LSPCEEEAPA SPAEDEHNQN GNLD
Length:534
Mass (Da):61,006
Last modified:June 1, 1994 - v1
Checksum:iC86C3F48E0AE9B69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94867 mRNA. Translation: AAA74558.1.
PIRiA44162.
RefSeqiNP_776840.1. NM_174415.2.
UniGeneiBt.3910.

Genome annotation databases

EnsembliENSBTAT00000003552; ENSBTAP00000003552; ENSBTAG00000004337.
GeneIDi281970.
KEGGibta:281970.

Similar proteinsi

Entry informationi

Entry nameiPDE1B_BOVIN
AccessioniPrimary (citable) accession number: Q01061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families