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Q01061

- PDE1B_BOVIN

UniProt

Q01061 - PDE1B_BOVIN

Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

PDE1B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate By similarity.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Type I PDE are activated by the binding of calmodulin in the presence of Ca2+. Regulated by phosphorylation by CaM-dependent protein kinase II.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211Proton donorBy similarity
    Metal bindingi225 – 2251Divalent metal cation 1By similarity
    Metal bindingi261 – 2611Divalent metal cation 1By similarity
    Metal bindingi262 – 2621Divalent metal cation 1By similarity
    Metal bindingi262 – 2621Divalent metal cation 2By similarity
    Metal bindingi368 – 3681Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
    2. calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. metabolic process Source: GOC
    2. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calmodulin-binding, cAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_205403. cGMP effects.
    REACT_206547. G alpha (s) signalling events.
    REACT_212942. Cam-PDE 1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
    Short name:
    Cam-PDE 1B
    Alternative name(s):
    63 kDa Cam-PDE
    Gene namesi
    Name:PDE1B
    Synonyms:PDE1B1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BPRO_0000198787Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei464 – 4641PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ01061.

    Expressioni

    Tissue specificityi

    Expressed in central nervous system regions. Most abundant in basal ganglia. Also found in kidney papilla and adrenal medulla.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi159261. 2 interactions.
    STRINGi9913.ENSBTAP00000005680.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01061.
    SMRiQ01061. Positions 144-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 4721Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni195 – 494300CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG139098.
    GeneTreeiENSGT00660000095451.
    HOGENOMiHOG000231888.
    HOVERGENiHBG056120.
    KOiK13755.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013706. PDEase_N.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    PF08499. PDEase_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01061-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV    50
    NIEELKKNLE YTASLLEAVY IDETRQILDT EDELQELRSD AVPSEVRDWL 100
    ASTFTQQTRA KGPSEEKPKF RSIVHAVQAG IFVERMFRRT YTSVGPTYST 150
    AVLNCLKNVD LWCFDVFSLN RAADDHALRT IVFELLTRHN LISRFKIPTV 200
    FLMTFLDALE TGYGKYKNPY HNQIHAADVT QTVHCFLLRT GMVHCLSEIE 250
    VLAIIFAAAI HDYEHTGTTN SFHIQTKSEC AILYNDRSVL ENHHISSVFR 300
    MMQDDEMNIF INLTKDEFVE LRALVIEMVL ATDMSCHFQQ VKSMKTALQQ 350
    LERIDKSKAL SLLLHAADIS HPTKQWSVHS RWTKALMEEF FRQGDKEAEL 400
    GLPFSPLCDR TSTLVAQSQI GFIDFIVEPT FSVLTDVAEK SVQPTGDDDS 450
    KSKNQPSFQW RQPSLDVEVG DPNPDVVSFR STWTKYIQEN KQKWKERAAS 500
    GITNQMSIDE LSPCEEEAPA SPAEDEHNQN GNLD 534
    Length:534
    Mass (Da):61,006
    Last modified:June 1, 1994 - v1
    Checksum:iC86C3F48E0AE9B69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94867 mRNA. Translation: AAA74558.1.
    PIRiA44162.
    RefSeqiNP_776840.1. NM_174415.2.
    UniGeneiBt.3910.

    Genome annotation databases

    EnsembliENSBTAT00000003552; ENSBTAP00000003552; ENSBTAG00000004337.
    GeneIDi281970.
    KEGGibta:281970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94867 mRNA. Translation: AAA74558.1 .
    PIRi A44162.
    RefSeqi NP_776840.1. NM_174415.2.
    UniGenei Bt.3910.

    3D structure databases

    ProteinModelPortali Q01061.
    SMRi Q01061. Positions 144-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 159261. 2 interactions.
    STRINGi 9913.ENSBTAP00000005680.

    Chemistry

    BindingDBi Q01061.
    ChEMBLi CHEMBL2111408.

    Proteomic databases

    PRIDEi Q01061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000003552 ; ENSBTAP00000003552 ; ENSBTAG00000004337 .
    GeneIDi 281970.
    KEGGi bta:281970.

    Organism-specific databases

    CTDi 5153.

    Phylogenomic databases

    eggNOGi NOG139098.
    GeneTreei ENSGT00660000095451.
    HOGENOMi HOG000231888.
    HOVERGENi HBG056120.
    KOi K13755.

    Enzyme and pathway databases

    Reactomei REACT_205403. cGMP effects.
    REACT_206547. G alpha (s) signalling events.
    REACT_212942. Cam-PDE 1 activation.

    Miscellaneous databases

    NextBioi 20805841.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013706. PDEase_N.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    PF08499. PDEase_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNA encoding a '63'-kDa calmodulin-stimulated phosphodiesterase from bovine brain."
      Bentley J.K., Kadlecek A., Sherbert C.H., Seger D., Sonnenburg W.K., Charbonneau H., Novack J.P., Beavo J.A.
      J. Biol. Chem. 267:18676-18682(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Sequence comparison of the 63-, 61-, and 59-kDa calmodulin-dependent cyclic nucleotide phosphodiesterases."
      Novack J.P., Charbonneau H., Bentley J.K., Walsh K.A., Beavo J.A.
      Biochemistry 30:7940-7947(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.

    Entry informationi

    Entry nameiPDE1B_BOVIN
    AccessioniPrimary (citable) accession number: Q01061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3