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Q01056

- DEN_SHV21

UniProt

Q01056 - DEN_SHV21

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Protein

Deneddylase

Gene

64

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331PROSITE-ProRule annotation
Active sitei163 – 1631PROSITE-ProRule annotation
Active sitei165 – 1651PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:64
Synonyms:EERF2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
ProteomesiUP000000587: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24692469DeneddylasePRO_0000116039Add
BLAST

Proteomic databases

PRIDEiQ01056.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ01056.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 225213Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili967 – 101448Sequence AnalysisAdd
BLAST
Coiled coili1285 – 132440Sequence AnalysisAdd
BLAST
Coiled coili1448 – 147528Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi258 – 31255Lys-richAdd
BLAST
Compositional biasi370 – 43465Asp-richAdd
BLAST
Compositional biasi2286 – 22894Poly-Asn

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01056-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDIHPLFKKL NLEGIASTHQ ADEKYGQYAG SQCLSNCVMF LVSSYYNDET
60 70 80 90 100
PVTSLHGLNE ILKYGAKIDF ILRRSGQLGH NQYAQLHHIP GYIAGPKWAC
110 120 130 140 150
FIYQSIEMFG MLGHESPINE PFVASLKSLL SKNYNTTVQY FLAICNSKSM
160 170 180 190 200
GILIKDKKIF IFDPHSCPLV PNSPAHVFST SNVNDAIEYL SPPNVQYTGS
210 220 230 240 250
FLYFVPKEYI GHSHYIMNHY RVINYEKLHG PNIDLTSQEG LIIEISPPNT
260 270 280 290 300
PKPTSTQKPP KTPRTPKPAT PKAPKTPRKP KTPKESTIPY DKSKKPPKIP
310 320 330 340 350
KTSKKSKKVL TKDTALTPQH KTIEEHLREL LPPITETVED NTLFNHPVER
360 370 380 390 400
TTPGTDSLLS GINSTTKRED DLEDDDNVTS KLKEDEDDWI DDIPIPEVLD
410 420 430 440 450
TETTHSDQET IYMIGDENIH DWSYSDDDID DTLDISFIQL DNLITSLDNI
460 470 480 490 500
PKNNTFPRII DKTSNQPIKE GKALHSIDRI LKNIVLEHGL ITSSSISISK
510 520 530 540 550
CKSLLQFVIL WGEKLSIPTR DLKTILKTEL IITEIAEIAL TKLTNDTFRN
560 570 580 590 600
NVITKLNKCM LKLKSESVDS YKHLSALLNN IILKIQTIDT EIELKTLSTV
610 620 630 640 650
FTSELGKDFS VVCTKKESET IMAAIKNLKE KISTRKQELH TEENYFQSVL
660 670 680 690 700
IAMETFQPIP LPTRVIEIQP SKKAQQLHEK SKLVEQKLTI DANNVLTDLL
710 720 730 740 750
HTMKQDKTDI SPAPDFTTVL KNIQSTLQLL QTCVTDLNID KKFISNTVQQ
760 770 780 790 800
LSYIGWEVAE LSHSQWNFPK ADPVIPLKIL DDIKKEIQQV TTKQKNEETL
810 820 830 840 850
SKILADVQTL LENAKQSDTL SIPILQHYIT KAGTLVGERE NQKFESLKNT
860 870 880 890 900
VQKLSTSEEF LKTLIDSTTL ENVQLQIQEI SDILQSNQYI HQSETIKQAF
910 920 930 940 950
FDKSNTIINN ILQLINQQKY TTVTQPMLIA VKRFLSEAKF RESNTICEII
960 970 980 990 1000
STLVSLGSLL SKSTTVEALK DALKSIDTLK EKLTAVDRPL KRELYNVIRK
1010 1020 1030 1040 1050
LQKQLKTLLE QQEFDNWKME VDSFVPTPSR DVKTFIQNAP SMKAKQYAKK
1060 1070 1080 1090 1100
ALKDQIQAME IDVDPESVIE DNIKANGQKA WQKIQSAFQD LNFSILIPDD
1110 1120 1130 1140 1150
WLSLAKEYTR PKSTLFTVIG PILLKFVEEV LESVKNLKEA KLKSLLPNGP
1160 1170 1180 1190 1200
VFTPPKFDWI HYYESNVNFH LKTINLPKVS TVAHNIGHEL SLLSQALNSK
1210 1220 1230 1240 1250
TLPEAVVGTS LEQHAAKFSC MFKTLEATWH DHQVDTRTKI DEYIEDLRND
1260 1270 1280 1290 1300
TKKHIVAPQI QSPNRFLSPE DIQEINSLPK LFRDSLLENE SRLLASQKNE
1310 1320 1330 1340 1350
FQMLENTVKA AELQYKATQE DIISNMSEAI NSLLPLAPAY ILAIPTIPTD
1360 1370 1380 1390 1400
PLKYVENIIQ DKRLLNTEPY QITIECLNWL NTACKTLLSI CPKSQKQRLV
1410 1420 1430 1440 1450
VLDQSINTHL NITQQFYNLE KTANTTDDLS VLQNAISTLD LKRVQGGKAT
1460 1470 1480 1490 1500
VDSWQSKLQQ MKAMLDNISK SAQTLASLDI LWGTALTSVS TAHLGELLQK
1510 1520 1530 1540 1550
ADPLQKDIES LSSTNTDLLS RVTELIHFIK FKRGFLSYYE EGQKEVFQRY
1560 1570 1580 1590 1600
PLTQNIRPSQ PTEINNLLRL ALFVLLKNKD ASAWIWTETL PLVDSNKLAY
1610 1620 1630 1640 1650
VPPNKGPLYT CSQYLKLLEA QLLDPSLSKV ILSDNRPLAG IAQARLGIDS
1660 1670 1680 1690 1700
TVLLARAFPD IQKHAEEVLT AYKNSIVSHT QNEFMAMTIV CHMIKIIMND
1710 1720 1730 1740 1750
FYPQNFNINT VPIYVNHTKL LQIILTMWPR LIKASLCQQS FQEATSLLQT
1760 1770 1780 1790 1800
TLKPLFLKIT DLTLENNIYN PASHCSDALL FFPQKWKSIN IQSIMWEHPS
1810 1820 1830 1840 1850
FLAICKNKSR ARITFLALAF KIIDPTILNQ LWTSLNPANT SDSTSYSLLL
1860 1870 1880 1890 1900
NHLVATEFDK NVPSTFLEPG NPSLAYAYGT QTGNIIGTKS YVPQKSPPIS
1910 1920 1930 1940 1950
VTAFEIALGA LIFQVPVKLF VTDKTPVLSS PELGDMLIVS ELLDCTGTTE
1960 1970 1980 1990 2000
PFKTMIEAPK SSLSTNLNKQ YVSPPHELEV FSRQASWLQH ILSSSNFKNN
2010 2020 2030 2040 2050
IVATIDYSTT FLNAYVVPEK LPFKQESFCF IPKIDSLQWP NNTFTTFLPL
2060 2070 2080 2090 2100
VEMPSNIELH YAKVTEPFNK TVLSTMFNVF PTHILPTQEE HDQSISSKSP
2110 2120 2130 2140 2150
TFKIEHDYNT NSVYNNHINN INLTNNSTYH QYKDVLPQPL ADKLSYEPKD
2160 2170 2180 2190 2200
LQNLASTTEP QIEDIFSELS IKETDNTAKA PLLYPQKQPK TKKFLSPVHT
2210 2220 2230 2240 2250
KHKTSNSIIF EENTTVKVQP NTCIQHSDLH KDTNTPRQQI SNAPCFIPNH
2260 2270 2280 2290 2300
KVPVIIKPSQ EKLKANTVHT NTDDLSPKKP QILIANNNNI FKQSDKQHKH
2310 2320 2330 2340 2350
QYTQISKPKI FINQDSNNPI KQPHHNPPQP LIKPTDPQQL TLSNDIISSD
2360 2370 2380 2390 2400
QTTKNLNVQR KPIIVIPNNN YALNQVQKLS NLPSIKTKPY ITLKDIQSNS
2410 2420 2430 2440 2450
KTLYDESPIT IPILEHLDIE PIVSISYLEK RVDETKFIIL EFIKHTKQNI
2460
IKTTNLLIHQ IMKIKTLYL
Length:2,469
Mass (Da):280,168
Last modified:April 1, 1993 - v1
Checksum:iD2B4B8DC08644CDB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45687.1.
M86409 Genomic DNA. Translation: AAA46140.1.
RefSeqiNP_040266.1. NC_001350.1.

Genome annotation databases

GeneIDi1682468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45687.1 .
M86409 Genomic DNA. Translation: AAA46140.1 .
RefSeqi NP_040266.1. NC_001350.1.

3D structure databases

ProteinModelPortali Q01056.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q01056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1682468.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the herpesvirus saimiri genome."
    Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
    J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
    Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
    Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDEN_SHV21
AccessioniPrimary (citable) accession number: Q01056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3