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Q01056 (DEN_SHV21) Reviewed, UniProtKB/Swiss-Prot

Last modified July 24, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:64
Synonyms:EERF2
OrganismSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri) [Reference proteome]
Taxonomic identifier10383 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]

Protein attributes

Sequence length2469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 24692469Deneddylase
PRO_0000116039

Regions

Domain13 – 225213Peptidase C76
Coiled coil967 – 101448 Potential
Coiled coil1285 – 132440 Potential
Coiled coil1448 – 147528 Potential
Compositional bias258 – 31255Lys-rich
Compositional bias370 – 43465Asp-rich
Compositional bias2286 – 22894Poly-Asn

Sites

Active site331 By similarity
Active site1631 By similarity
Active site1651 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q01056 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: D2B4B8DC08644CDB

FASTA2,469280,168
        10         20         30         40         50         60 
MDIHPLFKKL NLEGIASTHQ ADEKYGQYAG SQCLSNCVMF LVSSYYNDET PVTSLHGLNE 

        70         80         90        100        110        120 
ILKYGAKIDF ILRRSGQLGH NQYAQLHHIP GYIAGPKWAC FIYQSIEMFG MLGHESPINE 

       130        140        150        160        170        180 
PFVASLKSLL SKNYNTTVQY FLAICNSKSM GILIKDKKIF IFDPHSCPLV PNSPAHVFST 

       190        200        210        220        230        240 
SNVNDAIEYL SPPNVQYTGS FLYFVPKEYI GHSHYIMNHY RVINYEKLHG PNIDLTSQEG 

       250        260        270        280        290        300 
LIIEISPPNT PKPTSTQKPP KTPRTPKPAT PKAPKTPRKP KTPKESTIPY DKSKKPPKIP 

       310        320        330        340        350        360 
KTSKKSKKVL TKDTALTPQH KTIEEHLREL LPPITETVED NTLFNHPVER TTPGTDSLLS 

       370        380        390        400        410        420 
GINSTTKRED DLEDDDNVTS KLKEDEDDWI DDIPIPEVLD TETTHSDQET IYMIGDENIH 

       430        440        450        460        470        480 
DWSYSDDDID DTLDISFIQL DNLITSLDNI PKNNTFPRII DKTSNQPIKE GKALHSIDRI 

       490        500        510        520        530        540 
LKNIVLEHGL ITSSSISISK CKSLLQFVIL WGEKLSIPTR DLKTILKTEL IITEIAEIAL 

       550        560        570        580        590        600 
TKLTNDTFRN NVITKLNKCM LKLKSESVDS YKHLSALLNN IILKIQTIDT EIELKTLSTV 

       610        620        630        640        650        660 
FTSELGKDFS VVCTKKESET IMAAIKNLKE KISTRKQELH TEENYFQSVL IAMETFQPIP 

       670        680        690        700        710        720 
LPTRVIEIQP SKKAQQLHEK SKLVEQKLTI DANNVLTDLL HTMKQDKTDI SPAPDFTTVL 

       730        740        750        760        770        780 
KNIQSTLQLL QTCVTDLNID KKFISNTVQQ LSYIGWEVAE LSHSQWNFPK ADPVIPLKIL 

       790        800        810        820        830        840 
DDIKKEIQQV TTKQKNEETL SKILADVQTL LENAKQSDTL SIPILQHYIT KAGTLVGERE 

       850        860        870        880        890        900 
NQKFESLKNT VQKLSTSEEF LKTLIDSTTL ENVQLQIQEI SDILQSNQYI HQSETIKQAF 

       910        920        930        940        950        960 
FDKSNTIINN ILQLINQQKY TTVTQPMLIA VKRFLSEAKF RESNTICEII STLVSLGSLL 

       970        980        990       1000       1010       1020 
SKSTTVEALK DALKSIDTLK EKLTAVDRPL KRELYNVIRK LQKQLKTLLE QQEFDNWKME 

      1030       1040       1050       1060       1070       1080 
VDSFVPTPSR DVKTFIQNAP SMKAKQYAKK ALKDQIQAME IDVDPESVIE DNIKANGQKA 

      1090       1100       1110       1120       1130       1140 
WQKIQSAFQD LNFSILIPDD WLSLAKEYTR PKSTLFTVIG PILLKFVEEV LESVKNLKEA 

      1150       1160       1170       1180       1190       1200 
KLKSLLPNGP VFTPPKFDWI HYYESNVNFH LKTINLPKVS TVAHNIGHEL SLLSQALNSK 

      1210       1220       1230       1240       1250       1260 
TLPEAVVGTS LEQHAAKFSC MFKTLEATWH DHQVDTRTKI DEYIEDLRND TKKHIVAPQI 

      1270       1280       1290       1300       1310       1320 
QSPNRFLSPE DIQEINSLPK LFRDSLLENE SRLLASQKNE FQMLENTVKA AELQYKATQE 

      1330       1340       1350       1360       1370       1380 
DIISNMSEAI NSLLPLAPAY ILAIPTIPTD PLKYVENIIQ DKRLLNTEPY QITIECLNWL 

      1390       1400       1410       1420       1430       1440 
NTACKTLLSI CPKSQKQRLV VLDQSINTHL NITQQFYNLE KTANTTDDLS VLQNAISTLD 

      1450       1460       1470       1480       1490       1500 
LKRVQGGKAT VDSWQSKLQQ MKAMLDNISK SAQTLASLDI LWGTALTSVS TAHLGELLQK 

      1510       1520       1530       1540       1550       1560 
ADPLQKDIES LSSTNTDLLS RVTELIHFIK FKRGFLSYYE EGQKEVFQRY PLTQNIRPSQ 

      1570       1580       1590       1600       1610       1620 
PTEINNLLRL ALFVLLKNKD ASAWIWTETL PLVDSNKLAY VPPNKGPLYT CSQYLKLLEA 

      1630       1640       1650       1660       1670       1680 
QLLDPSLSKV ILSDNRPLAG IAQARLGIDS TVLLARAFPD IQKHAEEVLT AYKNSIVSHT 

      1690       1700       1710       1720       1730       1740 
QNEFMAMTIV CHMIKIIMND FYPQNFNINT VPIYVNHTKL LQIILTMWPR LIKASLCQQS 

      1750       1760       1770       1780       1790       1800 
FQEATSLLQT TLKPLFLKIT DLTLENNIYN PASHCSDALL FFPQKWKSIN IQSIMWEHPS 

      1810       1820       1830       1840       1850       1860 
FLAICKNKSR ARITFLALAF KIIDPTILNQ LWTSLNPANT SDSTSYSLLL NHLVATEFDK 

      1870       1880       1890       1900       1910       1920 
NVPSTFLEPG NPSLAYAYGT QTGNIIGTKS YVPQKSPPIS VTAFEIALGA LIFQVPVKLF 

      1930       1940       1950       1960       1970       1980 
VTDKTPVLSS PELGDMLIVS ELLDCTGTTE PFKTMIEAPK SSLSTNLNKQ YVSPPHELEV 

      1990       2000       2010       2020       2030       2040 
FSRQASWLQH ILSSSNFKNN IVATIDYSTT FLNAYVVPEK LPFKQESFCF IPKIDSLQWP 

      2050       2060       2070       2080       2090       2100 
NNTFTTFLPL VEMPSNIELH YAKVTEPFNK TVLSTMFNVF PTHILPTQEE HDQSISSKSP 

      2110       2120       2130       2140       2150       2160 
TFKIEHDYNT NSVYNNHINN INLTNNSTYH QYKDVLPQPL ADKLSYEPKD LQNLASTTEP 

      2170       2180       2190       2200       2210       2220 
QIEDIFSELS IKETDNTAKA PLLYPQKQPK TKKFLSPVHT KHKTSNSIIF EENTTVKVQP 

      2230       2240       2250       2260       2270       2280 
NTCIQHSDLH KDTNTPRQQI SNAPCFIPNH KVPVIIKPSQ EKLKANTVHT NTDDLSPKKP 

      2290       2300       2310       2320       2330       2340 
QILIANNNNI FKQSDKQHKH QYTQISKPKI FINQDSNNPI KQPHHNPPQP LIKPTDPQQL 

      2350       2360       2370       2380       2390       2400 
TLSNDIISSD QTTKNLNVQR KPIIVIPNNN YALNQVQKLS NLPSIKTKPY ITLKDIQSNS 

      2410       2420       2430       2440       2450       2460 
KTLYDESPIT IPILEHLDIE PIVSISYLEK RVDETKFIIL EFIKHTKQNI IKTTNLLIHQ 


IMKIKTLYL 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the herpesvirus saimiri genome."
Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64346 Genomic DNA. Translation: CAA45687.1.
M86409 Genomic DNA. Translation: AAA46140.1.
RefSeqNP_040266.1. NC_001350.1.

3D structure databases

ProteinModelPortalQ01056.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ01056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1682468.

Phylogenomic databases

ProtClustDBCLSP2509700.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_SHV21
AccessionPrimary (citable) accession number: Q01056
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 24, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries