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Q01056

- DEN_SHV21

UniProt

Q01056 - DEN_SHV21

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Protein
Deneddylase
Gene
64, EERF2
Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331 By similarity
Active sitei163 – 1631 By similarity
Active sitei165 – 1651 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:64
Synonyms:EERF2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
ProteomesiUP000000587: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24692469Deneddylase
PRO_0000116039Add
BLAST

Proteomic databases

PRIDEiQ01056.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ01056.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 225213Peptidase C76
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili967 – 101448 Reviewed prediction
Add
BLAST
Coiled coili1285 – 132440 Reviewed prediction
Add
BLAST
Coiled coili1448 – 147528 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi258 – 31255Lys-rich
Add
BLAST
Compositional biasi370 – 43465Asp-rich
Add
BLAST
Compositional biasi2286 – 22894Poly-Asn

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01056-1 [UniParc]FASTAAdd to Basket

« Hide

MDIHPLFKKL NLEGIASTHQ ADEKYGQYAG SQCLSNCVMF LVSSYYNDET     50
PVTSLHGLNE ILKYGAKIDF ILRRSGQLGH NQYAQLHHIP GYIAGPKWAC 100
FIYQSIEMFG MLGHESPINE PFVASLKSLL SKNYNTTVQY FLAICNSKSM 150
GILIKDKKIF IFDPHSCPLV PNSPAHVFST SNVNDAIEYL SPPNVQYTGS 200
FLYFVPKEYI GHSHYIMNHY RVINYEKLHG PNIDLTSQEG LIIEISPPNT 250
PKPTSTQKPP KTPRTPKPAT PKAPKTPRKP KTPKESTIPY DKSKKPPKIP 300
KTSKKSKKVL TKDTALTPQH KTIEEHLREL LPPITETVED NTLFNHPVER 350
TTPGTDSLLS GINSTTKRED DLEDDDNVTS KLKEDEDDWI DDIPIPEVLD 400
TETTHSDQET IYMIGDENIH DWSYSDDDID DTLDISFIQL DNLITSLDNI 450
PKNNTFPRII DKTSNQPIKE GKALHSIDRI LKNIVLEHGL ITSSSISISK 500
CKSLLQFVIL WGEKLSIPTR DLKTILKTEL IITEIAEIAL TKLTNDTFRN 550
NVITKLNKCM LKLKSESVDS YKHLSALLNN IILKIQTIDT EIELKTLSTV 600
FTSELGKDFS VVCTKKESET IMAAIKNLKE KISTRKQELH TEENYFQSVL 650
IAMETFQPIP LPTRVIEIQP SKKAQQLHEK SKLVEQKLTI DANNVLTDLL 700
HTMKQDKTDI SPAPDFTTVL KNIQSTLQLL QTCVTDLNID KKFISNTVQQ 750
LSYIGWEVAE LSHSQWNFPK ADPVIPLKIL DDIKKEIQQV TTKQKNEETL 800
SKILADVQTL LENAKQSDTL SIPILQHYIT KAGTLVGERE NQKFESLKNT 850
VQKLSTSEEF LKTLIDSTTL ENVQLQIQEI SDILQSNQYI HQSETIKQAF 900
FDKSNTIINN ILQLINQQKY TTVTQPMLIA VKRFLSEAKF RESNTICEII 950
STLVSLGSLL SKSTTVEALK DALKSIDTLK EKLTAVDRPL KRELYNVIRK 1000
LQKQLKTLLE QQEFDNWKME VDSFVPTPSR DVKTFIQNAP SMKAKQYAKK 1050
ALKDQIQAME IDVDPESVIE DNIKANGQKA WQKIQSAFQD LNFSILIPDD 1100
WLSLAKEYTR PKSTLFTVIG PILLKFVEEV LESVKNLKEA KLKSLLPNGP 1150
VFTPPKFDWI HYYESNVNFH LKTINLPKVS TVAHNIGHEL SLLSQALNSK 1200
TLPEAVVGTS LEQHAAKFSC MFKTLEATWH DHQVDTRTKI DEYIEDLRND 1250
TKKHIVAPQI QSPNRFLSPE DIQEINSLPK LFRDSLLENE SRLLASQKNE 1300
FQMLENTVKA AELQYKATQE DIISNMSEAI NSLLPLAPAY ILAIPTIPTD 1350
PLKYVENIIQ DKRLLNTEPY QITIECLNWL NTACKTLLSI CPKSQKQRLV 1400
VLDQSINTHL NITQQFYNLE KTANTTDDLS VLQNAISTLD LKRVQGGKAT 1450
VDSWQSKLQQ MKAMLDNISK SAQTLASLDI LWGTALTSVS TAHLGELLQK 1500
ADPLQKDIES LSSTNTDLLS RVTELIHFIK FKRGFLSYYE EGQKEVFQRY 1550
PLTQNIRPSQ PTEINNLLRL ALFVLLKNKD ASAWIWTETL PLVDSNKLAY 1600
VPPNKGPLYT CSQYLKLLEA QLLDPSLSKV ILSDNRPLAG IAQARLGIDS 1650
TVLLARAFPD IQKHAEEVLT AYKNSIVSHT QNEFMAMTIV CHMIKIIMND 1700
FYPQNFNINT VPIYVNHTKL LQIILTMWPR LIKASLCQQS FQEATSLLQT 1750
TLKPLFLKIT DLTLENNIYN PASHCSDALL FFPQKWKSIN IQSIMWEHPS 1800
FLAICKNKSR ARITFLALAF KIIDPTILNQ LWTSLNPANT SDSTSYSLLL 1850
NHLVATEFDK NVPSTFLEPG NPSLAYAYGT QTGNIIGTKS YVPQKSPPIS 1900
VTAFEIALGA LIFQVPVKLF VTDKTPVLSS PELGDMLIVS ELLDCTGTTE 1950
PFKTMIEAPK SSLSTNLNKQ YVSPPHELEV FSRQASWLQH ILSSSNFKNN 2000
IVATIDYSTT FLNAYVVPEK LPFKQESFCF IPKIDSLQWP NNTFTTFLPL 2050
VEMPSNIELH YAKVTEPFNK TVLSTMFNVF PTHILPTQEE HDQSISSKSP 2100
TFKIEHDYNT NSVYNNHINN INLTNNSTYH QYKDVLPQPL ADKLSYEPKD 2150
LQNLASTTEP QIEDIFSELS IKETDNTAKA PLLYPQKQPK TKKFLSPVHT 2200
KHKTSNSIIF EENTTVKVQP NTCIQHSDLH KDTNTPRQQI SNAPCFIPNH 2250
KVPVIIKPSQ EKLKANTVHT NTDDLSPKKP QILIANNNNI FKQSDKQHKH 2300
QYTQISKPKI FINQDSNNPI KQPHHNPPQP LIKPTDPQQL TLSNDIISSD 2350
QTTKNLNVQR KPIIVIPNNN YALNQVQKLS NLPSIKTKPY ITLKDIQSNS 2400
KTLYDESPIT IPILEHLDIE PIVSISYLEK RVDETKFIIL EFIKHTKQNI 2450
IKTTNLLIHQ IMKIKTLYL 2469
Length:2,469
Mass (Da):280,168
Last modified:April 1, 1993 - v1
Checksum:iD2B4B8DC08644CDB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45687.1.
M86409 Genomic DNA. Translation: AAA46140.1.
RefSeqiNP_040266.1. NC_001350.1.

Genome annotation databases

GeneIDi1682468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45687.1 .
M86409 Genomic DNA. Translation: AAA46140.1 .
RefSeqi NP_040266.1. NC_001350.1.

3D structure databases

ProteinModelPortali Q01056.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q01056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1682468.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the herpesvirus saimiri genome."
    Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
    J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
    Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
    Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDEN_SHV21
AccessioniPrimary (citable) accession number: Q01056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi