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Q01056

- DEN_SHV21

UniProt

Q01056 - DEN_SHV21

Protein

Deneddylase

Gene

64

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei33 – 331PROSITE-ProRule annotation
    Active sitei163 – 1631PROSITE-ProRule annotation
    Active sitei165 – 1651PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:64
    Synonyms:EERF2
    OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
    Taxonomic identifieri10383 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
    Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
    ProteomesiUP000000587: Genome

    Subcellular locationi

    Virion tegument. Host cytoplasm. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24692469DeneddylasePRO_0000116039Add
    BLAST

    Proteomic databases

    PRIDEiQ01056.

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ01056.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 225213Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili967 – 101448Sequence AnalysisAdd
    BLAST
    Coiled coili1285 – 132440Sequence AnalysisAdd
    BLAST
    Coiled coili1448 – 147528Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi258 – 31255Lys-richAdd
    BLAST
    Compositional biasi370 – 43465Asp-richAdd
    BLAST
    Compositional biasi2286 – 22894Poly-Asn

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIHPLFKKL NLEGIASTHQ ADEKYGQYAG SQCLSNCVMF LVSSYYNDET     50
    PVTSLHGLNE ILKYGAKIDF ILRRSGQLGH NQYAQLHHIP GYIAGPKWAC 100
    FIYQSIEMFG MLGHESPINE PFVASLKSLL SKNYNTTVQY FLAICNSKSM 150
    GILIKDKKIF IFDPHSCPLV PNSPAHVFST SNVNDAIEYL SPPNVQYTGS 200
    FLYFVPKEYI GHSHYIMNHY RVINYEKLHG PNIDLTSQEG LIIEISPPNT 250
    PKPTSTQKPP KTPRTPKPAT PKAPKTPRKP KTPKESTIPY DKSKKPPKIP 300
    KTSKKSKKVL TKDTALTPQH KTIEEHLREL LPPITETVED NTLFNHPVER 350
    TTPGTDSLLS GINSTTKRED DLEDDDNVTS KLKEDEDDWI DDIPIPEVLD 400
    TETTHSDQET IYMIGDENIH DWSYSDDDID DTLDISFIQL DNLITSLDNI 450
    PKNNTFPRII DKTSNQPIKE GKALHSIDRI LKNIVLEHGL ITSSSISISK 500
    CKSLLQFVIL WGEKLSIPTR DLKTILKTEL IITEIAEIAL TKLTNDTFRN 550
    NVITKLNKCM LKLKSESVDS YKHLSALLNN IILKIQTIDT EIELKTLSTV 600
    FTSELGKDFS VVCTKKESET IMAAIKNLKE KISTRKQELH TEENYFQSVL 650
    IAMETFQPIP LPTRVIEIQP SKKAQQLHEK SKLVEQKLTI DANNVLTDLL 700
    HTMKQDKTDI SPAPDFTTVL KNIQSTLQLL QTCVTDLNID KKFISNTVQQ 750
    LSYIGWEVAE LSHSQWNFPK ADPVIPLKIL DDIKKEIQQV TTKQKNEETL 800
    SKILADVQTL LENAKQSDTL SIPILQHYIT KAGTLVGERE NQKFESLKNT 850
    VQKLSTSEEF LKTLIDSTTL ENVQLQIQEI SDILQSNQYI HQSETIKQAF 900
    FDKSNTIINN ILQLINQQKY TTVTQPMLIA VKRFLSEAKF RESNTICEII 950
    STLVSLGSLL SKSTTVEALK DALKSIDTLK EKLTAVDRPL KRELYNVIRK 1000
    LQKQLKTLLE QQEFDNWKME VDSFVPTPSR DVKTFIQNAP SMKAKQYAKK 1050
    ALKDQIQAME IDVDPESVIE DNIKANGQKA WQKIQSAFQD LNFSILIPDD 1100
    WLSLAKEYTR PKSTLFTVIG PILLKFVEEV LESVKNLKEA KLKSLLPNGP 1150
    VFTPPKFDWI HYYESNVNFH LKTINLPKVS TVAHNIGHEL SLLSQALNSK 1200
    TLPEAVVGTS LEQHAAKFSC MFKTLEATWH DHQVDTRTKI DEYIEDLRND 1250
    TKKHIVAPQI QSPNRFLSPE DIQEINSLPK LFRDSLLENE SRLLASQKNE 1300
    FQMLENTVKA AELQYKATQE DIISNMSEAI NSLLPLAPAY ILAIPTIPTD 1350
    PLKYVENIIQ DKRLLNTEPY QITIECLNWL NTACKTLLSI CPKSQKQRLV 1400
    VLDQSINTHL NITQQFYNLE KTANTTDDLS VLQNAISTLD LKRVQGGKAT 1450
    VDSWQSKLQQ MKAMLDNISK SAQTLASLDI LWGTALTSVS TAHLGELLQK 1500
    ADPLQKDIES LSSTNTDLLS RVTELIHFIK FKRGFLSYYE EGQKEVFQRY 1550
    PLTQNIRPSQ PTEINNLLRL ALFVLLKNKD ASAWIWTETL PLVDSNKLAY 1600
    VPPNKGPLYT CSQYLKLLEA QLLDPSLSKV ILSDNRPLAG IAQARLGIDS 1650
    TVLLARAFPD IQKHAEEVLT AYKNSIVSHT QNEFMAMTIV CHMIKIIMND 1700
    FYPQNFNINT VPIYVNHTKL LQIILTMWPR LIKASLCQQS FQEATSLLQT 1750
    TLKPLFLKIT DLTLENNIYN PASHCSDALL FFPQKWKSIN IQSIMWEHPS 1800
    FLAICKNKSR ARITFLALAF KIIDPTILNQ LWTSLNPANT SDSTSYSLLL 1850
    NHLVATEFDK NVPSTFLEPG NPSLAYAYGT QTGNIIGTKS YVPQKSPPIS 1900
    VTAFEIALGA LIFQVPVKLF VTDKTPVLSS PELGDMLIVS ELLDCTGTTE 1950
    PFKTMIEAPK SSLSTNLNKQ YVSPPHELEV FSRQASWLQH ILSSSNFKNN 2000
    IVATIDYSTT FLNAYVVPEK LPFKQESFCF IPKIDSLQWP NNTFTTFLPL 2050
    VEMPSNIELH YAKVTEPFNK TVLSTMFNVF PTHILPTQEE HDQSISSKSP 2100
    TFKIEHDYNT NSVYNNHINN INLTNNSTYH QYKDVLPQPL ADKLSYEPKD 2150
    LQNLASTTEP QIEDIFSELS IKETDNTAKA PLLYPQKQPK TKKFLSPVHT 2200
    KHKTSNSIIF EENTTVKVQP NTCIQHSDLH KDTNTPRQQI SNAPCFIPNH 2250
    KVPVIIKPSQ EKLKANTVHT NTDDLSPKKP QILIANNNNI FKQSDKQHKH 2300
    QYTQISKPKI FINQDSNNPI KQPHHNPPQP LIKPTDPQQL TLSNDIISSD 2350
    QTTKNLNVQR KPIIVIPNNN YALNQVQKLS NLPSIKTKPY ITLKDIQSNS 2400
    KTLYDESPIT IPILEHLDIE PIVSISYLEK RVDETKFIIL EFIKHTKQNI 2450
    IKTTNLLIHQ IMKIKTLYL 2469
    Length:2,469
    Mass (Da):280,168
    Last modified:April 1, 1993 - v1
    Checksum:iD2B4B8DC08644CDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64346 Genomic DNA. Translation: CAA45687.1.
    M86409 Genomic DNA. Translation: AAA46140.1.
    RefSeqiNP_040266.1. NC_001350.1.

    Genome annotation databases

    GeneIDi1682468.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64346 Genomic DNA. Translation: CAA45687.1 .
    M86409 Genomic DNA. Translation: AAA46140.1 .
    RefSeqi NP_040266.1. NC_001350.1.

    3D structure databases

    ProteinModelPortali Q01056.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q01056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1682468.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the herpesvirus saimiri genome."
      Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
      J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
      Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
      Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_SHV21
    AccessioniPrimary (citable) accession number: Q01056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3