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Q01037

- RIR1_SHV21

UniProt

Q01037 - RIR1_SHV21

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

61

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei176 – 1761SubstrateBy similarity
Sitei192 – 1921Important for hydrogen atom transferBy similarity
Binding sitei222 – 2221Substrate; via amide nitrogenBy similarity
Active sitei392 – 3921Proton acceptorBy similarity
Active sitei394 – 3941Cysteine radical intermediateBy similarity
Active sitei396 – 3961Proton acceptorBy similarity
Sitei408 – 4081Important for hydrogen atom transferBy similarity
Sitei709 – 7091Important for electron transferBy similarity
Sitei710 – 7101Important for electron transferBy similarity
Sitei763 – 7631Interacts with thioredoxin/glutaredoxinBy similarity
Sitei766 – 7661Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:61
Synonyms:EELF2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
ProteomesiUP000000587: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Ribonucleoside-diphosphate reductase large subunitPRO_0000187243Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi192 ↔ 408Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ01037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1922Substrate bindingBy similarity
Regioni392 – 3965Substrate bindingBy similarity
Regioni578 – 5825Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01037-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQETIISNL IDMLKVSAGW DREANEISGR LFHKLMDMSS TETISQYMSL
60 70 80 90 100
FGPLLEPHIL EFIQNYEQEI DEVCLEYRAS YDFMCLRNCG ILPAKRFYDT
110 120 130 140 150
YVLPPRTEMN GKYESIPHFF ARIAAYCAWN CIMCEPLKDT LVYVQKRDWN
160 170 180 190 200
VEIKTDMQIF KYFYKVISSQ LVCCATPVMR SAGVAGENLS SCFIIAPTLD
210 220 230 240 250
TEKSTISSIF GELAPLLASR SGVGVDVTKF SFGGKNIHSC LKLINAQVEF
260 270 280 290 300
FNDKSVRPVS VATYIEVWHC QIHEFLSAKL PENPDRCNSI FQGVCVPSLF
310 320 330 340 350
FKMYESDPNG LWYLFDPQDA PNLTRLYGLE FEEEYLRLVS EKKYKQSVTL
360 370 380 390 400
KSLMFSLINT IIKTGSPYVI SKEAMNKHHW YETQGEAINC SNLCAEIVQQ
410 420 430 440 450
PKQFTSTCNL ANVCLPKCLN SSNFPYTCSN TAQFDFSKLE YAVQAAVFII
460 470 480 490 500
NACILSPSPT SSATVGQRER SMGIGCHGLA DVFSEMGYGY LDLESECLDR
510 520 530 540 550
DIFETMYYTA VKTSSEICSV GKGQPFAGFR KSKLAHGVFH WATWDAMPQR
560 570 580 590 600
VPMKQWIHLQ DNIKKFGVFN SQFIALMPTA GTSQLTGYTD SFYPYFANMS
610 620 630 640 650
SKVSNKEEIM KPNITFLKNV KPQDLCTVRF YGGDVSMMPE DVSTRYKHFL
660 670 680 690 700
TAFDYCPEAQ MRRASIRAPY VDQSQSLTLF LTEENVQSAK YLKDLLLLGF
710 720 730 740 750
RLGLKTIMYY CRVKKTTKLL QLECLKLDEH TKKDAQIVLA DLARELPDSH
760
KTEDACPLDQ SECIACQ
Length:767
Mass (Da):87,216
Last modified:April 1, 1993 - v1
Checksum:iFE5922B11FFCA6DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45684.1.
M86409 Genomic DNA. Translation: AAA46137.1.
RefSeqiNP_040263.1. NC_001350.1.

Genome annotation databases

GeneIDi1682460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45684.1 .
M86409 Genomic DNA. Translation: AAA46137.1 .
RefSeqi NP_040263.1. NC_001350.1.

3D structure databases

ProteinModelPortali Q01037.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1682460.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the herpesvirus saimiri genome."
    Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
    J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
    Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
    Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_SHV21
AccessioniPrimary (citable) accession number: Q01037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3