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Q01037

- RIR1_SHV21

UniProt

Q01037 - RIR1_SHV21

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

61

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei176 – 1761SubstrateBy similarity
    Sitei192 – 1921Important for hydrogen atom transferBy similarity
    Binding sitei222 – 2221Substrate; via amide nitrogenBy similarity
    Active sitei392 – 3921Proton acceptorBy similarity
    Active sitei394 – 3941Cysteine radical intermediateBy similarity
    Active sitei396 – 3961Proton acceptorBy similarity
    Sitei408 – 4081Important for hydrogen atom transferBy similarity
    Sitei709 – 7091Important for electron transferBy similarity
    Sitei710 – 7101Important for electron transferBy similarity
    Sitei763 – 7631Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei766 – 7661Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase large subunit
    Gene namesi
    Name:61
    Synonyms:EELF2
    OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
    Taxonomic identifieri10383 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
    Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
    ProteomesiUP000000587: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 767767Ribonucleoside-diphosphate reductase large subunitPRO_0000187243Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi192 ↔ 408Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ01037.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni191 – 1922Substrate bindingBy similarity
    Regioni392 – 3965Substrate bindingBy similarity
    Regioni578 – 5825Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01037-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQETIISNL IDMLKVSAGW DREANEISGR LFHKLMDMSS TETISQYMSL    50
    FGPLLEPHIL EFIQNYEQEI DEVCLEYRAS YDFMCLRNCG ILPAKRFYDT 100
    YVLPPRTEMN GKYESIPHFF ARIAAYCAWN CIMCEPLKDT LVYVQKRDWN 150
    VEIKTDMQIF KYFYKVISSQ LVCCATPVMR SAGVAGENLS SCFIIAPTLD 200
    TEKSTISSIF GELAPLLASR SGVGVDVTKF SFGGKNIHSC LKLINAQVEF 250
    FNDKSVRPVS VATYIEVWHC QIHEFLSAKL PENPDRCNSI FQGVCVPSLF 300
    FKMYESDPNG LWYLFDPQDA PNLTRLYGLE FEEEYLRLVS EKKYKQSVTL 350
    KSLMFSLINT IIKTGSPYVI SKEAMNKHHW YETQGEAINC SNLCAEIVQQ 400
    PKQFTSTCNL ANVCLPKCLN SSNFPYTCSN TAQFDFSKLE YAVQAAVFII 450
    NACILSPSPT SSATVGQRER SMGIGCHGLA DVFSEMGYGY LDLESECLDR 500
    DIFETMYYTA VKTSSEICSV GKGQPFAGFR KSKLAHGVFH WATWDAMPQR 550
    VPMKQWIHLQ DNIKKFGVFN SQFIALMPTA GTSQLTGYTD SFYPYFANMS 600
    SKVSNKEEIM KPNITFLKNV KPQDLCTVRF YGGDVSMMPE DVSTRYKHFL 650
    TAFDYCPEAQ MRRASIRAPY VDQSQSLTLF LTEENVQSAK YLKDLLLLGF 700
    RLGLKTIMYY CRVKKTTKLL QLECLKLDEH TKKDAQIVLA DLARELPDSH 750
    KTEDACPLDQ SECIACQ 767
    Length:767
    Mass (Da):87,216
    Last modified:April 1, 1993 - v1
    Checksum:iFE5922B11FFCA6DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64346 Genomic DNA. Translation: CAA45684.1.
    M86409 Genomic DNA. Translation: AAA46137.1.
    RefSeqiNP_040263.1. NC_001350.1.

    Genome annotation databases

    GeneIDi1682460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64346 Genomic DNA. Translation: CAA45684.1 .
    M86409 Genomic DNA. Translation: AAA46137.1 .
    RefSeqi NP_040263.1. NC_001350.1.

    3D structure databases

    ProteinModelPortali Q01037.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1682460.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the herpesvirus saimiri genome."
      Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
      J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
      Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
      Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_SHV21
    AccessioniPrimary (citable) accession number: Q01037
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3