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Q01037

- RIR1_SHV21

UniProt

Q01037 - RIR1_SHV21

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Protein
Ribonucleoside-diphosphate reductase large subunit
Gene
61, EELF2
Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei176 – 1761Substrate By similarity
Sitei192 – 1921Important for hydrogen atom transfer By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Active sitei392 – 3921Proton acceptor By similarity
Active sitei394 – 3941Cysteine radical intermediate By similarity
Active sitei396 – 3961Proton acceptor By similarity
Sitei408 – 4081Important for hydrogen atom transfer By similarity
Sitei709 – 7091Important for electron transfer By similarity
Sitei710 – 7101Important for electron transfer By similarity
Sitei763 – 7631Interacts with thioredoxin/glutaredoxin By similarity
Sitei766 – 7661Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:61
Synonyms:EELF2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
ProteomesiUP000000587: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Ribonucleoside-diphosphate reductase large subunit
PRO_0000187243Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi192 ↔ 408Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ01037.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1922Substrate binding By similarity
Regioni392 – 3965Substrate binding By similarity
Regioni578 – 5825Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01037-1 [UniParc]FASTAAdd to Basket

« Hide

MSQETIISNL IDMLKVSAGW DREANEISGR LFHKLMDMSS TETISQYMSL    50
FGPLLEPHIL EFIQNYEQEI DEVCLEYRAS YDFMCLRNCG ILPAKRFYDT 100
YVLPPRTEMN GKYESIPHFF ARIAAYCAWN CIMCEPLKDT LVYVQKRDWN 150
VEIKTDMQIF KYFYKVISSQ LVCCATPVMR SAGVAGENLS SCFIIAPTLD 200
TEKSTISSIF GELAPLLASR SGVGVDVTKF SFGGKNIHSC LKLINAQVEF 250
FNDKSVRPVS VATYIEVWHC QIHEFLSAKL PENPDRCNSI FQGVCVPSLF 300
FKMYESDPNG LWYLFDPQDA PNLTRLYGLE FEEEYLRLVS EKKYKQSVTL 350
KSLMFSLINT IIKTGSPYVI SKEAMNKHHW YETQGEAINC SNLCAEIVQQ 400
PKQFTSTCNL ANVCLPKCLN SSNFPYTCSN TAQFDFSKLE YAVQAAVFII 450
NACILSPSPT SSATVGQRER SMGIGCHGLA DVFSEMGYGY LDLESECLDR 500
DIFETMYYTA VKTSSEICSV GKGQPFAGFR KSKLAHGVFH WATWDAMPQR 550
VPMKQWIHLQ DNIKKFGVFN SQFIALMPTA GTSQLTGYTD SFYPYFANMS 600
SKVSNKEEIM KPNITFLKNV KPQDLCTVRF YGGDVSMMPE DVSTRYKHFL 650
TAFDYCPEAQ MRRASIRAPY VDQSQSLTLF LTEENVQSAK YLKDLLLLGF 700
RLGLKTIMYY CRVKKTTKLL QLECLKLDEH TKKDAQIVLA DLARELPDSH 750
KTEDACPLDQ SECIACQ 767
Length:767
Mass (Da):87,216
Last modified:April 1, 1993 - v1
Checksum:iFE5922B11FFCA6DF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45684.1.
M86409 Genomic DNA. Translation: AAA46137.1.
RefSeqiNP_040263.1. NC_001350.1.

Genome annotation databases

GeneIDi1682460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64346 Genomic DNA. Translation: CAA45684.1 .
M86409 Genomic DNA. Translation: AAA46137.1 .
RefSeqi NP_040263.1. NC_001350.1.

3D structure databases

ProteinModelPortali Q01037.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1682460.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the herpesvirus saimiri genome."
    Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
    J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus."
    Nicholas J., Cameron K.R., Coleman H., Newman C., Honess R.W.
    Virology 188:296-310(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_SHV21
AccessioniPrimary (citable) accession number: Q01037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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