Q00U23 (Q00U23_OSTTA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Acetolactate synthase RuleBase RU003591
EC=2.2.1.6 RuleBase RU003591

Gene names

Ordered Locus Names:

Ot16g02270 EMBL CAL58226.1

Organism

Ostreococcus tauri [Complete proteome] EMBL CAL58226.1

Taxonomic identifier

70448 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Mamiellophyceae › Mamiellales › Ostreococcus

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Sequence similarities	Belongs to the TPP enzyme family. RuleBase RU003702
Caution	The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. EMBL CAL58226.1

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis RuleBase RU003591
Ligand	Magnesium RuleBase RU003591 Metal-binding RuleBase RU003591 Thiamine pyrophosphate RuleBase RU003591
Molecular function	Transferase RuleBase RU003591
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q00U23 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 48CFA490D4655C0A	FASTA	679	74,211
	10 20 30 40 50 60 MHANARAQRT PTTALSVQNA RVSRRAAIRA RSQIRRVLSL KSPAVREPGA SEYVDPSQFT 70 80 90 100 110 120 ELELTTSQMT AYASRRPIGH FQSRFADDEP RKGADILVEA LEREGVDCVF AYPGGASMEI 130 140 150 160 170 180 HQSLTKSVTG IRNVLCRHEQ GEVFAAEGYA KSTGKVGVCI ATSGPGATNL VTGLADALLD 190 200 210 220 230 240 SVPMVAITGQ VPRPMIGTDA FQETPIVEIT RQITKHNYLV MNVEEIPRIV REAFFLASSG 250 260 270 280 290 300 RPGPVLIDIP KDVQQQLAVP YWEPKVSLHG YLSRLPGPPT NPQIQQVVDL IKKSKKPIVY 310 320 330 340 350 360 AGGGCLDASK ELVEFCRATG IPVTNTLMGL GTFPQSDDLS LSMLGMHGAV YANYAIDSAD 370 380 390 400 410 420 LLLAFGVRFD DRVTGKLAEF AKRAAIVHID IDPAELGKNK KPFCSIFSNI KPALRSLNKL 430 440 450 460 470 480 IALDAANLPD FSDWRAEIEV KRKEFPFPIP KQQGDVIAPQ AAIELLCKLT EGKAIISTGV 490 500 510 520 530 540 GQHQMWAAQH YKFDEPRNWL SSGGLGSMGF GLPAAIGAQA ARPDELVIDI DGDGSFVMNI 550 560 570 580 590 600 QELATLHAEE LPVKCFILNN QYLGMVVQWE DRFYKANRGH TYLGSPNDDY QVSKEEAHIF 610 620 630 640 650 660 PDFVTISNGF RVPAERVTRM SELEGAIKRM IETPGPYLLD VMVPHVEHVL PMVPGGGTFA 670 DTIVTGDGRT PEEKDMKTL

References

[1]

"Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features." Derelle E., Ferraz C., Rombauts S., Rouze P., Worden A.Z., Robbens S., Partensky F., Degroeve S., Echeynie S., Cooke R., Saeys Y., Wuyts J., Jabbari K., Bowler C., Panaud O., Piegu B., Ball S.G., Ral J.-P. , Bouget F.-Y., Piganeau G., De Baets B., Picard A., Delseny M., Demaille J., Van de Peer Y., Moreau H. Proc. Natl. Acad. Sci. U.S.A. 103:11647-11652(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OTTH0595.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAID01000016 Genomic DNA. Translation: CAL58226.1.
RefSeq	XP_003083677.1. XM_003083629.1.
3D structure databases
ProteinModelPortal	Q00U23.
SMR	Q00U23. Positions 81-669.
ModBase	Search...
Protein-protein interaction databases
STRING	70448.Q00U23. Q00U23.
Proteomic databases
PRIDE	Q00U23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9831116.
KEGG	ota:Ot16g02270.
Phylogenomic databases
eggNOG	COG0028.
HOGENOM	HOG000258448.
KO	K01652.
OMA	NNEEQGM.
Enzyme and pathway databases
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q00U23_OSTTA

Accession

Primary (citable) accession number: Q00U23

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)