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Q00IB6 (CPL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II C-terminal domain phosphatase-like 4

Short name=FCP-like 4
EC=3.1.3.16
Alternative name(s):
Carboxyl-terminal phosphatase-like 4
Short name=AtCPL4
Short name=CTD phosphatase-like 4
Gene names
Name:CPL4
Ordered Locus Names:At5g58003
ORF Names:MTI20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II By similarity. Required for normal plant growth. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds magnesium, cobalt or manganese ions By similarity.

Subunit structure

Interacts with RAP74. Ref.1

Subcellular location

Nucleus Ref.1.

Induction

By NaCl. Ref.1

Domain

The BRCT domain is required for interaction with RAP74. Ref.1

Sequence similarities

Contains 1 BRCT domain.

Contains 1 FCP1 homology domain.

Sequence caution

The sequence BAB08870.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g58000 and At5g58003.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440RNA polymerase II C-terminal domain phosphatase-like 4
PRO_0000376086

Regions

Domain118 – 292175FCP1 homology
Domain337 – 42993BRCT

Sequences

Sequence LengthMass (Da)Tools
Q00IB6 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 7712641DC888B82B

FASTA44050,264
        10         20         30         40         50         60 
MSVASDSPVH SSSSSDDLAA FLDAELDSAS DASSGPSEEE EAEDDVESGL KRQKLEHLEE 

        70         80         90        100        110        120 
ASSSKGECEH PGSFGNMCFV CGQKLEETGV SFRYIHKEMR LNEDEISRLR DSDSRFLQRQ 

       130        140        150        160        170        180 
RKLYLVLDLD HTLLNTTILR DLKPEEEYLK SHTHSLQDGC NVSGGSLFLL EFMQMMTKLR 

       190        200        210        220        230        240 
PFVHSFLKEA SEMFVMYIYT MGDRNYARQM AKLLDPKGEY FGDRVISRDD GTVRHEKSLD 

       250        260        270        280        290        300 
VVLGQESAVL ILDDTENAWP KHKDNLIVIE RYHFFSSSCR QFDHRYKSLS ELKSDESEPD 

       310        320        330        340        350        360 
GALATVLKVL KQAHALFFEN VDEGISNRDV RLMLKQVRKE ILKGCKIVFS RVFPTKAKPE 

       370        380        390        400        410        420 
DHPLWKMAEE LGATCATEVD ASVTHVVAMD VGTEKARWAV REKKYVVHRG WIDAANYLWM 

       430        440 
KQPEENFGLE QLKKQLTEEE 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions."
Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M., Bahk J., Koiwa H.
Plant Physiol. 142:586-594(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAP74, INDUCTION BY NACL, BRCT DOMAIN, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
Kerk D., Templeton G., Moorhead G.B.G.
Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ503426 mRNA. Translation: ABF55959.1.
AB013396 Genomic DNA. Translation: BAB08870.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96984.1.
RefSeqNP_001078764.1. NM_001085295.1.
UniGeneAt.71283.

3D structure databases

ProteinModelPortalQ00IB6.
SMRQ00IB6. Positions 97-430.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid624522. 1 interaction.
STRING3702.AT5G58003.1-P.

Proteomic databases

PaxDbQ00IB6.
PRIDEQ00IB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G58003.1; AT5G58003.1; AT5G58003.
GeneID5008312.
KEGGath:AT5G58003.

Organism-specific databases

TAIRAT5G58003.

Phylogenomic databases

eggNOGCOG5190.
HOGENOMHOG000239575.
OMAQEEDFPV.
PhylomeDBQ00IB6.

Enzyme and pathway databases

BioCycARA:AT5G58003-MONOMER.

Gene expression databases

GenevestigatorQ00IB6.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR02250. FCP1_euk. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ00IB6.

Entry information

Entry nameCPL4_ARATH
AccessionPrimary (citable) accession number: Q00IB6
Secondary accession number(s): Q9FJL6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names