Reviewed, UniProtKB/Swiss-Prot Q00IB6 (CPL4_ARATH)

Names and origin

Protein names

Recommended name:
    RNA polymerase II C-terminal domain phosphatase-like 4
      Short name=FCP-like 4
    EC=3.1.3.16
Alternative name(s):
    Carboxyl-terminal phosphatase-like 4
      Short name=CTD phosphatase-like 4
      Short name=AtCPL4

Gene names

Name:	CPL4
Ordered Locus Names:	At5g58003

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II By similarity. Required for normal plant growth.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds magnesium, cobalt or manganese ions By similarity.
Subunit structure	Interacts with RAP74. Ref.1
Subcellular location	Nucleus. Ref.1
Induction	By NaCl. Ref.1
Domain	The BRCT domain is required for interaction with RAP74. Ref.1
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.
Sequence caution	The sequence BAB08870.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g58000 and At5g58003.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Metal-binding RNA-binding
Molecular function	Hydrolase Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW response to salt stress Ref.1 Inferred from expression pattern. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: EC protein C-terminus binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	RNA polymerase II C-terminal domain phosphatase-like 4		PRO_0000376086
Regions
	Domain	118 – 292	175	FCP1 homology
	Domain	337 – 429	93	BRCT

Sequences

	Sequence		Length	Mass (Da)
	Q00IB6-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 7712641DC888B82B	FASTA	440	50,264
	10 20 30 40 50 60 MSVASDSPVH SSSSSDDLAA FLDAELDSAS DASSGPSEEE EAEDDVESGL KRQKLEHLEE 70 80 90 100 110 120 ASSSKGECEH PGSFGNMCFV CGQKLEETGV SFRYIHKEMR LNEDEISRLR DSDSRFLQRQ 130 140 150 160 170 180 RKLYLVLDLD HTLLNTTILR DLKPEEEYLK SHTHSLQDGC NVSGGSLFLL EFMQMMTKLR 190 200 210 220 230 240 PFVHSFLKEA SEMFVMYIYT MGDRNYARQM AKLLDPKGEY FGDRVISRDD GTVRHEKSLD 250 260 270 280 290 300 VVLGQESAVL ILDDTENAWP KHKDNLIVIE RYHFFSSSCR QFDHRYKSLS ELKSDESEPD 310 320 330 340 350 360 GALATVLKVL KQAHALFFEN VDEGISNRDV RLMLKQVRKE ILKGCKIVFS RVFPTKAKPE 370 380 390 400 410 420 DHPLWKMAEE LGATCATEVD ASVTHVVAMD VGTEKARWAV REKKYVVHRG WIDAANYLWM 430 440 KQPEENFGLE QLKKQLTEEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions." Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M., Bahk J., Koiwa H. Plant Physiol. 142:586-594(2006) [PubMed: 16905668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAP74, INDUCTION BY NACL, BRCT DOMAIN, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants." Kerk D., Templeton G., Moorhead G.B.G. Plant Physiol. 146:351-367(2008) [PubMed: 18156295] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ503426 mRNA. Translation: ABF55959.1. AB013396 Genomic DNA. Translation: BAB08870.1. Sequence problems.
IPI	IPI00570463.
RefSeq	NP_001078764.1.
UniGene	At.71283
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q00IB6.
Genome annotation databases
GeneID	5008312.
GenomeReviews	Gene locus AT5G58003 in contig BA000015_GR.
KEGG	ath:AT5G58003.
Organism-specific databases
TAIR	At5g58003.
Gene expression databases
Genevestigator	Q00IB6.
Family and domain databases
InterPro	IPR001357. BRCT. IPR011947. FCP1_euk. IPR004274. NIF. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPL4_ARATH

Accession

Primary (citable) accession number: Q00IB6
Secondary accession number(s): Q9FJL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	November 14, 2006
Last modified:	October 13, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)