Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA polymerase II C-terminal domain phosphatase-like 4

Gene

CPL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II (By similarity). Required for normal plant growth.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Co2+By similarity, Mn2+By similarityNote: Binds Mg2+, Co2+ or Mn2+.By similarity

GO - Molecular functioni

  • CTD phosphatase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • protein C-terminus binding Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G58003-MONOMER.
ReactomeiR-ATH-112382. Formation of RNA Pol II elongation complex.
R-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 4 (EC:3.1.3.16)
Short name:
FCP-like 4
Alternative name(s):
Carboxyl-terminal phosphatase-like 4
Short name:
AtCPL4
Short name:
CTD phosphatase-like 4
Gene namesi
Name:CPL4
Ordered Locus Names:At5g58003
ORF Names:MTI20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G58003.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase II, holoenzyme Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440RNA polymerase II C-terminal domain phosphatase-like 4PRO_0000376086Add
BLAST

Proteomic databases

PaxDbiQ00IB6.
PRIDEiQ00IB6.

Expressioni

Inductioni

By NaCl.1 Publication

Gene expression databases

GenevisibleiQ00IB6. AT.

Interactioni

Subunit structurei

Interacts with RAP74.1 Publication

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi624522. 4 interactions.
STRINGi3702.AT5G58003.1.

Structurei

3D structure databases

ProteinModelPortaliQ00IB6.
SMRiQ00IB6. Positions 343-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 292175FCP1 homologyPROSITE-ProRule annotationAdd
BLAST
Domaini337 – 42993BRCTPROSITE-ProRule annotationAdd
BLAST

Domaini

The BRCT domain is required for interaction with RAP74.

Sequence similaritiesi

Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0323. Eukaryota.
COG5190. LUCA.
HOGENOMiHOG000239575.
InParanoidiQ00IB6.
KOiK18999.
OMAiHALFFEN.
OrthoDBiEOG09360I0G.
PhylomeDBiQ00IB6.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR004274. FCP1_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00IB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVASDSPVH SSSSSDDLAA FLDAELDSAS DASSGPSEEE EAEDDVESGL
60 70 80 90 100
KRQKLEHLEE ASSSKGECEH PGSFGNMCFV CGQKLEETGV SFRYIHKEMR
110 120 130 140 150
LNEDEISRLR DSDSRFLQRQ RKLYLVLDLD HTLLNTTILR DLKPEEEYLK
160 170 180 190 200
SHTHSLQDGC NVSGGSLFLL EFMQMMTKLR PFVHSFLKEA SEMFVMYIYT
210 220 230 240 250
MGDRNYARQM AKLLDPKGEY FGDRVISRDD GTVRHEKSLD VVLGQESAVL
260 270 280 290 300
ILDDTENAWP KHKDNLIVIE RYHFFSSSCR QFDHRYKSLS ELKSDESEPD
310 320 330 340 350
GALATVLKVL KQAHALFFEN VDEGISNRDV RLMLKQVRKE ILKGCKIVFS
360 370 380 390 400
RVFPTKAKPE DHPLWKMAEE LGATCATEVD ASVTHVVAMD VGTEKARWAV
410 420 430 440
REKKYVVHRG WIDAANYLWM KQPEENFGLE QLKKQLTEEE
Length:440
Mass (Da):50,264
Last modified:November 14, 2006 - v1
Checksum:i7712641DC888B82B
GO

Sequence cautioni

The sequence BAB08870 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g58000 and At5g58003.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503426 mRNA. Translation: ABF55959.1.
AB013396 Genomic DNA. Translation: BAB08870.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96984.1.
RefSeqiNP_001078764.1. NM_001085295.1.
UniGeneiAt.71283.

Genome annotation databases

EnsemblPlantsiAT5G58003.1; AT5G58003.1; AT5G58003.
GeneIDi5008312.
GrameneiAT5G58003.1; AT5G58003.1; AT5G58003.
KEGGiath:AT5G58003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503426 mRNA. Translation: ABF55959.1.
AB013396 Genomic DNA. Translation: BAB08870.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96984.1.
RefSeqiNP_001078764.1. NM_001085295.1.
UniGeneiAt.71283.

3D structure databases

ProteinModelPortaliQ00IB6.
SMRiQ00IB6. Positions 343-431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi624522. 4 interactions.
STRINGi3702.AT5G58003.1.

Proteomic databases

PaxDbiQ00IB6.
PRIDEiQ00IB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58003.1; AT5G58003.1; AT5G58003.
GeneIDi5008312.
GrameneiAT5G58003.1; AT5G58003.1; AT5G58003.
KEGGiath:AT5G58003.

Organism-specific databases

TAIRiAT5G58003.

Phylogenomic databases

eggNOGiKOG0323. Eukaryota.
COG5190. LUCA.
HOGENOMiHOG000239575.
InParanoidiQ00IB6.
KOiK18999.
OMAiHALFFEN.
OrthoDBiEOG09360I0G.
PhylomeDBiQ00IB6.

Enzyme and pathway databases

BioCyciARA:AT5G58003-MONOMER.
ReactomeiR-ATH-112382. Formation of RNA Pol II elongation complex.
R-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Miscellaneous databases

PROiQ00IB6.

Gene expression databases

GenevisibleiQ00IB6. AT.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR004274. FCP1_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPL4_ARATH
AccessioniPrimary (citable) accession number: Q00IB6
Secondary accession number(s): Q9FJL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 14, 2006
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.