Q00IB6 (CPL4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 4
Short name=FCP-like 4
EC=3.1.3.16

Alternative name(s):
Carboxyl-terminal phosphatase-like 4
Short name=AtCPL4
Short name=CTD phosphatase-like 4

Gene names

Name:	CPL4
Ordered Locus Names:	At5g58003
ORF Names:	MTI20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II By similarity. Required for normal plant growth. Ref.1
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds magnesium, cobalt or manganese ions By similarity.
Subunit structure	Interacts with RAP74. Ref.1
Subcellular location	Nucleus Ref.1.
Induction	By NaCl. Ref.1
Domain	The BRCT domain is required for interaction with RAP74. Ref.1
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.
Sequence caution	The sequence BAB08870.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g58000 and At5g58003.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Metal-binding RNA-binding
Molecular function	Hydrolase Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to salt stress Inferred from expression pattern Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	RNA polymerase II C-terminal domain phosphatase-like 4		PRO_0000376086
Regions
	Domain	118 – 292	175	FCP1 homology
	Domain	337 – 429	93	BRCT

Sequences

	Sequence		Length	Mass (Da)
	Q00IB6 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 7712641DC888B82B	FASTA	440	50,264
	10 20 30 40 50 60 MSVASDSPVH SSSSSDDLAA FLDAELDSAS DASSGPSEEE EAEDDVESGL KRQKLEHLEE 70 80 90 100 110 120 ASSSKGECEH PGSFGNMCFV CGQKLEETGV SFRYIHKEMR LNEDEISRLR DSDSRFLQRQ 130 140 150 160 170 180 RKLYLVLDLD HTLLNTTILR DLKPEEEYLK SHTHSLQDGC NVSGGSLFLL EFMQMMTKLR 190 200 210 220 230 240 PFVHSFLKEA SEMFVMYIYT MGDRNYARQM AKLLDPKGEY FGDRVISRDD GTVRHEKSLD 250 260 270 280 290 300 VVLGQESAVL ILDDTENAWP KHKDNLIVIE RYHFFSSSCR QFDHRYKSLS ELKSDESEPD 310 320 330 340 350 360 GALATVLKVL KQAHALFFEN VDEGISNRDV RLMLKQVRKE ILKGCKIVFS RVFPTKAKPE 370 380 390 400 410 420 DHPLWKMAEE LGATCATEVD ASVTHVVAMD VGTEKARWAV REKKYVVHRG WIDAANYLWM 430 440 KQPEENFGLE QLKKQLTEEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions." Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M., Bahk J., Koiwa H. Plant Physiol. 142:586-594(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAP74, INDUCTION BY NACL, BRCT DOMAIN, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants." Kerk D., Templeton G., Moorhead G.B.G. Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ503426 mRNA. Translation: ABF55959.1. AB013396 Genomic DNA. Translation: BAB08870.1. Sequence problems. CP002688 Genomic DNA. Translation: AED96984.1.
IPI	IPI00570463.
RefSeq	NP_001078764.1. NM_001085295.1.
UniGene	At.71283.
3D structure databases
ProteinModelPortal	Q00IB6.
SMR	Q00IB6. Positions 340-430.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G58003.1-P.
Proteomic databases
PaxDb	Q00IB6.
PRIDE	Q00IB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G58003.1; AT5G58003.1; AT5G58003.
GeneID	5008312.
KEGG	ath:AT5G58003.
Organism-specific databases
TAIR	At5g58003.
Phylogenomic databases
eggNOG	COG5190.
HOGENOM	HOG000239575.
OMA	ILDDTEY.
PhylomeDB	Q00IB6.
ProtClustDB	CLSN2699127.
Gene expression databases
Genevestigator	Q00IB6.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit. 3.40.50.10190. 1 hit.
InterPro	IPR001357. BRCT_dom. IPR011947. FCP1_euk. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPL4_ARATH

Accession

Primary (citable) accession number: Q00IB6
Secondary accession number(s): Q9FJL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	November 14, 2006
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program