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Protein

RNA polymerase II C-terminal domain phosphatase-like 4

Gene

CPL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II (By similarity). Required for normal plant growth.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Co2+By similarity, Mn2+By similarityNote: Binds Mg2+, Co2+ or Mn2+.By similarity

GO - Molecular functioni

  • CTD phosphatase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • protein C-terminus binding Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Repressor, RNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-ATH-113418 Formation of the Early Elongation Complex
R-ATH-674695 RNA Polymerase II Pre-transcription Events
R-ATH-6796648 TP53 Regulates Transcription of DNA Repair Genes

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 4 (EC:3.1.3.16)
Short name:
FCP-like 4
Alternative name(s):
Carboxyl-terminal phosphatase-like 4
Short name:
AtCPL4
Short name:
CTD phosphatase-like 4
Gene namesi
Name:CPL4
Ordered Locus Names:At5g58003
ORF Names:MTI20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT5G58003
TAIRilocus:4010714056 AT5G58003

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003760861 – 440RNA polymerase II C-terminal domain phosphatase-like 4Add BLAST440

Proteomic databases

PaxDbiQ00IB6
PRIDEiQ00IB6

Expressioni

Inductioni

By NaCl.1 Publication

Gene expression databases

ExpressionAtlasiQ00IB6 baseline and differential
GenevisibleiQ00IB6 AT

Interactioni

Subunit structurei

Interacts with RAP74.1 Publication

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi624522, 4 interactors
STRINGi3702.AT5G58003.1

Structurei

3D structure databases

ProteinModelPortaliQ00IB6
SMRiQ00IB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini118 – 292FCP1 homologyPROSITE-ProRule annotationAdd BLAST175
Domaini337 – 429BRCTPROSITE-ProRule annotationAdd BLAST93

Domaini

The BRCT domain is required for interaction with RAP74.

Phylogenomic databases

eggNOGiKOG0323 Eukaryota
COG5190 LUCA
HOGENOMiHOG000239575
InParanoidiQ00IB6
KOiK18999
OMAiRVEDQHR
OrthoDBiEOG09360I0G
PhylomeDBiQ00IB6

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.1000, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR004274 FCP1_dom
IPR011947 FCP1_euk
IPR036412 HAD-like_sf
IPR023214 HAD_sf
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00577 CPDc, 1 hit
SUPFAMiSSF52113 SSF52113, 1 hit
SSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02250 FCP1_euk, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

Q00IB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVASDSPVH SSSSSDDLAA FLDAELDSAS DASSGPSEEE EAEDDVESGL
60 70 80 90 100
KRQKLEHLEE ASSSKGECEH PGSFGNMCFV CGQKLEETGV SFRYIHKEMR
110 120 130 140 150
LNEDEISRLR DSDSRFLQRQ RKLYLVLDLD HTLLNTTILR DLKPEEEYLK
160 170 180 190 200
SHTHSLQDGC NVSGGSLFLL EFMQMMTKLR PFVHSFLKEA SEMFVMYIYT
210 220 230 240 250
MGDRNYARQM AKLLDPKGEY FGDRVISRDD GTVRHEKSLD VVLGQESAVL
260 270 280 290 300
ILDDTENAWP KHKDNLIVIE RYHFFSSSCR QFDHRYKSLS ELKSDESEPD
310 320 330 340 350
GALATVLKVL KQAHALFFEN VDEGISNRDV RLMLKQVRKE ILKGCKIVFS
360 370 380 390 400
RVFPTKAKPE DHPLWKMAEE LGATCATEVD ASVTHVVAMD VGTEKARWAV
410 420 430 440
REKKYVVHRG WIDAANYLWM KQPEENFGLE QLKKQLTEEE
Length:440
Mass (Da):50,264
Last modified:November 14, 2006 - v1
Checksum:i7712641DC888B82B
GO

Sequence cautioni

The sequence BAB08870 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g58000 and At5g58003.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503426 mRNA Translation: ABF55959.1
AB013396 Genomic DNA Translation: BAB08870.1 Sequence problems.
CP002688 Genomic DNA Translation: AED96984.1
RefSeqiNP_001078764.1, NM_001085295.2
UniGeneiAt.71283

Genome annotation databases

EnsemblPlantsiAT5G58003.1; AT5G58003.1; AT5G58003
GeneIDi5008312
GrameneiAT5G58003.1; AT5G58003.1; AT5G58003
KEGGiath:AT5G58003

Entry informationi

Entry nameiCPL4_ARATH
AccessioniPrimary (citable) accession number: Q00IB6
Secondary accession number(s): Q9FJL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 14, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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