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Protein

Protein BEX3

Gene

NGFRAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiREACT_13526. NADE modulates death signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BEX3
Alternative name(s):
Brain-expressed X-linked protein 3
Nerve growth factor receptor-associated protein 1
Ovarian granulosa cell 13.0 kDa protein HGR74
p75NTR-associated cell death executor
Gene namesi
Name:NGFRAP1
Synonyms:BEX3, DXS6984E, NADE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:13388. NGFRAP1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Shuttles between the cytoplasm and the nucleus. Associates with replicating mitochondria (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31616.

Polymorphism and mutation databases

DMDMi547642.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 111111Protein BEX3PRO_0000096688Add
BLAST

Post-translational modificationi

Ubiquitinated. Degraded by the proteasome (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ00994.
PRIDEiQ00994.

Expressioni

Tissue specificityi

Found in ovarian granulosa cells, testis, prostate and seminal vesicle tissue. High levels also detected in liver.1 Publication

Gene expression databases

BgeeiQ00994.
CleanExiHS_NGFRAP1.
GenevisibleiQ00994. HS.

Organism-specific databases

HPAiHPA018886.

Interactioni

Subunit structurei

Self-associates. Interacts with 14-3-3 epsilon (YWHAE). Interacts with DIABLO/SMAC (By similarity). Binds to the DEATH domain of p75NTR/NGFR.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC116Q8IYX34EBI-741753,EBI-744311
CTNNBL1Q8WYA63EBI-741753,EBI-748128
EMILIN1Q9Y6C23EBI-741753,EBI-744586
FSD2A1L4K13EBI-741753,EBI-5661036
GOLGA8DPQ0D2H93EBI-741753,EBI-10181276
GOLGA8GQ08AF83EBI-741753,EBI-10181260
NECAB2H3BTW23EBI-741753,EBI-10172876
TSC1Q925745EBI-741753,EBI-1047085
USHBP1Q8N6Y03EBI-741753,EBI-739895

Protein-protein interaction databases

BioGridi117956. 19 interactions.
IntActiQ00994. 20 interactions.
MINTiMINT-1471079.
STRINGi9606.ENSP00000299872.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SA6model-A1-111[»]
ProteinModelPortaliQ00994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 11144Interaction with 14-3-3 epsilonBy similarityAdd
BLAST
Regioni68 – 9326Interaction with p75NTR/NGFRBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 8711Nuclear export signalBy similarityAdd
BLAST

Domaini

The nuclear export signal is required for export from the nucleus and the interactions with itself and p75NTR/NGFR.By similarity

Sequence similaritiesi

Belongs to the BEX family.Curated

Phylogenomic databases

eggNOGiNOG48134.
GeneTreeiENSGT00740000115473.
HOGENOMiHOG000236300.
HOVERGENiHBG080240.
InParanoidiQ00994.
OMAiLIMANIH.
OrthoDBiEOG7T1RF2.
PhylomeDBiQ00994.
TreeFamiTF337909.

Family and domain databases

InterProiIPR007623. BEX.
IPR021156. TF_A-like/BEX-like.
[Graphical view]
PfamiPF04538. BEX. 1 hit.
[Graphical view]
PIRSFiPIRSF008633. BEX. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00994-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANIHQENEE MEQPMQNGEE DRPLGGGEGH QPAGNRRGQA RRLAPNFRWA
60 70 80 90 100
IPNRQINDGM GGDGDDMEIF MEEMREIRRK LRELQLRNCL RILMGELSNH
110
HDHHDEFCLM P
Length:111
Mass (Da):12,959
Last modified:June 1, 1994 - v1
Checksum:i29AA0573282C933E
GO
Isoform 2 (identifier: Q00994-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.
Show »
Length:101
Mass (Da):11,762
Checksum:iED263FF37D8E838F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010Missing in isoform 2. 1 PublicationVSP_046343

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38188 mRNA. Translation: AAA63232.1.
AF187064 mRNA. Translation: AAF75129.1.
AY833562 mRNA. Translation: AAX40680.1.
AK315371 mRNA. Translation: BAG37764.1.
EL953547 Genomic DNA. No translation available.
AL606763 Genomic DNA. No translation available.
CH471190 Genomic DNA. Translation: EAW54710.1.
CH471190 Genomic DNA. Translation: EAW54712.1.
BC003190 mRNA. Translation: AAH03190.1.
CCDSiCCDS14508.1. [Q00994-1]
CCDS14509.1. [Q00994-2]
PIRiC35826.
RefSeqiNP_055195.1. NM_014380.2. [Q00994-1]
NP_996798.1. NM_206915.2. [Q00994-1]
NP_996800.1. NM_206917.2. [Q00994-2]
UniGeneiHs.448588.

Genome annotation databases

EnsembliENST00000361298; ENSP00000354843; ENSG00000166681. [Q00994-2]
ENST00000372634; ENSP00000361717; ENSG00000166681. [Q00994-2]
ENST00000372635; ENSP00000361718; ENSG00000166681.
ENST00000372645; ENSP00000361728; ENSG00000166681.
GeneIDi27018.
UCSCiuc004ekh.3. human. [Q00994-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38188 mRNA. Translation: AAA63232.1.
AF187064 mRNA. Translation: AAF75129.1.
AY833562 mRNA. Translation: AAX40680.1.
AK315371 mRNA. Translation: BAG37764.1.
EL953547 Genomic DNA. No translation available.
AL606763 Genomic DNA. No translation available.
CH471190 Genomic DNA. Translation: EAW54710.1.
CH471190 Genomic DNA. Translation: EAW54712.1.
BC003190 mRNA. Translation: AAH03190.1.
CCDSiCCDS14508.1. [Q00994-1]
CCDS14509.1. [Q00994-2]
PIRiC35826.
RefSeqiNP_055195.1. NM_014380.2. [Q00994-1]
NP_996798.1. NM_206915.2. [Q00994-1]
NP_996800.1. NM_206917.2. [Q00994-2]
UniGeneiHs.448588.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SA6model-A1-111[»]
ProteinModelPortaliQ00994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117956. 19 interactions.
IntActiQ00994. 20 interactions.
MINTiMINT-1471079.
STRINGi9606.ENSP00000299872.

Polymorphism and mutation databases

DMDMi547642.

Proteomic databases

PaxDbiQ00994.
PRIDEiQ00994.

Protocols and materials databases

DNASUi27018.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361298; ENSP00000354843; ENSG00000166681. [Q00994-2]
ENST00000372634; ENSP00000361717; ENSG00000166681. [Q00994-2]
ENST00000372635; ENSP00000361718; ENSG00000166681.
ENST00000372645; ENSP00000361728; ENSG00000166681.
GeneIDi27018.
UCSCiuc004ekh.3. human. [Q00994-1]

Organism-specific databases

CTDi27018.
GeneCardsiGC0XP102631.
HGNCiHGNC:13388. NGFRAP1.
HPAiHPA018886.
MIMi300361. gene.
neXtProtiNX_Q00994.
PharmGKBiPA31616.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG48134.
GeneTreeiENSGT00740000115473.
HOGENOMiHOG000236300.
HOVERGENiHBG080240.
InParanoidiQ00994.
OMAiLIMANIH.
OrthoDBiEOG7T1RF2.
PhylomeDBiQ00994.
TreeFamiTF337909.

Enzyme and pathway databases

ReactomeiREACT_13526. NADE modulates death signalling.

Miscellaneous databases

ChiTaRSiNGFRAP1. human.
GeneWikiiNGFRAP1.
GenomeRNAii27018.
NextBioi49532.
PROiQ00994.
SOURCEiSearch...

Gene expression databases

BgeeiQ00994.
CleanExiHS_NGFRAP1.
GenevisibleiQ00994. HS.

Family and domain databases

InterProiIPR007623. BEX.
IPR021156. TF_A-like/BEX-like.
[Graphical view]
PfamiPF04538. BEX. 1 hit.
[Graphical view]
PIRSFiPIRSF008633. BEX. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of three abundant mRNAs from human ovarian granulosa cells."
    Rapp G., Freudenstein J., Klaudiny J., Mucha J., Wempe F., Zimmer M., Scheit K.H.
    DNA Cell Biol. 9:479-485(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Ovary.
  2. "NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR."
    Mukai J., Hachiya T., Shoji-Hoshino S., Kimura M.T., Nadano D., Suvanto P., Hanaoka T., Li Y., Irie S., Greene L.A., Sato T.-A.
    J. Biol. Chem. 275:17566-17570(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
  3. "Characterization of the Bex gene family in humans, mice, and rats."
    Alvarez E., Zhou W., Witta S.E., Freed C.R.
    Gene 357:18-28(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Cerebellum.
  5. "A comparative gene expression profile of the whole eye from human, mouse, and guinea pig."
    Zhou X., Wang W., Lu F., Hu S., Jiang L., Yan D., Zhang X., Yu X., Yu J., Qu J.
    Mol. Vis. 13:2214-2221(2007)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Eye.
  6. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.
  9. "Theoretical model for p75NTR-associated cell death executor."
    Arunkumar S.
    Submitted (JAN-2005) to the PDB data bank
    Cited for: 3D-STRUCTURE MODELING OF 1-111.

Entry informationi

Entry nameiBEX3_HUMAN
AccessioniPrimary (citable) accession number: Q00994
Secondary accession number(s): B2RD17
, D3DXA3, Q5JQT4, Q5JQT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 22, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Binds transition metals.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.