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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

Uchl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
Sitei176 – 1761Important for enzyme activityBy similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: Ensembl
  2. ligase activity Source: UniProtKB-KW
  3. omega peptidase activity Source: Ensembl
  4. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. axon target recognition Source: Ensembl
  3. axon transport of mitochondrion Source: Ensembl
  4. cell proliferation Source: Ensembl
  5. eating behavior Source: Ensembl
  6. muscle fiber development Source: Ensembl
  7. negative regulation of MAP kinase activity Source: Ensembl
  8. neuromuscular process Source: Ensembl
  9. protein deubiquitination Source: Ensembl
  10. response to ischemia Source: Ensembl
  11. sensory perception of pain Source: RGD
  12. ubiquitin-dependent protein catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.19.12. 5301.

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:Uchl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3928. Uchl1.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. extracellular vesicular exosome Source: Ensembl
  5. neuronal cell body Source: Ensembl
  6. nucleus Source: Ensembl
  7. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211060Add
BLAST
Propeptidei221 – 2233Removed in mature formBy similarityPRO_0000414315

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteineBy similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Prenylation

Proteomic databases

PaxDbiQ00981.
PRIDEiQ00981.

2D gel databases

World-2DPAGE0004:Q00981.

PTM databases

PhosphoSiteiQ00981.

Expressioni

Gene expression databases

GenevestigatoriQ00981.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 (By similarity). Interacts with SNCA.By similarity1 Publication

Protein-protein interaction databases

BioGridi248179. 1 interaction.
IntActiQ00981. 1 interaction.
STRINGi10116.ENSRNOP00000056108.

Structurei

3D structure databases

ProteinModelPortaliQ00981.
SMRiQ00981. Positions 1-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitinBy similarity
Regioni151 – 1566Interaction with ubiquitinBy similarity
Regioni211 – 2166Interaction with ubiquitinBy similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiQ00981.
KOiK05611.
OMAiMEWKPME.
OrthoDBiEOG7S7SFK.
PhylomeDBiQ00981.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR030297. UCHL1.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PTHR10589:SF19. PTHR10589:SF19. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLAKL GVAGQWRFAD VLGLEEETLG SVPSPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDSVAQEG
160 170 180 190 200
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV
210 220
CREFTEREQG EVRFSAVALC KAA
Length:223
Mass (Da):24,838
Last modified:July 5, 2005 - v2
Checksum:iF1402BF7B0C077EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381T → I in BAA01541 (PubMed:1331034).Curated
Sequence conflicti96 – 961I → M in BAA01541 (PubMed:1331034).Curated
Sequence conflicti105 – 1073KLE → NLG in BAA01541 (PubMed:1331034).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10699 mRNA. Translation: BAA01541.1.
BC060573 mRNA. Translation: AAH60573.1.
PIRiJX0222.
RefSeqiNP_058933.2. NM_017237.3.
UniGeneiRn.107213.

Genome annotation databases

EnsembliENSRNOT00000003248; ENSRNOP00000003248; ENSRNOG00000002343.
GeneIDi29545.
KEGGirno:29545.
UCSCiRGD:3928. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10699 mRNA. Translation: BAA01541.1.
BC060573 mRNA. Translation: AAH60573.1.
PIRiJX0222.
RefSeqiNP_058933.2. NM_017237.3.
UniGeneiRn.107213.

3D structure databases

ProteinModelPortaliQ00981.
SMRiQ00981. Positions 1-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248179. 1 interaction.
IntActiQ00981. 1 interaction.
STRINGi10116.ENSRNOP00000056108.

Chemistry

BindingDBiQ00981.

Protein family/group databases

MEROPSiC12.001.

PTM databases

PhosphoSiteiQ00981.

2D gel databases

World-2DPAGE0004:Q00981.

Proteomic databases

PaxDbiQ00981.
PRIDEiQ00981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003248; ENSRNOP00000003248; ENSRNOG00000002343.
GeneIDi29545.
KEGGirno:29545.
UCSCiRGD:3928. rat.

Organism-specific databases

CTDi7345.
RGDi3928. Uchl1.

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiQ00981.
KOiK05611.
OMAiMEWKPME.
OrthoDBiEOG7S7SFK.
PhylomeDBiQ00981.

Enzyme and pathway databases

BRENDAi3.4.19.12. 5301.

Miscellaneous databases

NextBioi609557.
PROiQ00981.

Gene expression databases

GenevestigatoriQ00981.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR030297. UCHL1.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PTHR10589:SF19. PTHR10589:SF19. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and tissue distribution of a rat ubiquitin carboxyl-terminal hydrolase PGP9.5."
    Kajimoto Y., Hashimoto T., Shirai Y., Nishino N., Kuno T., Tanaka C.
    J. Biochem. 112:28-32(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. Lubec G., Boddul S., Afjehi-Sadat L., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 20-27; 84-123 AND 132-199, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Spinal cord.
  4. "Cytosolic O-glycosylation is abundant in nerve terminals."
    Cole R.N., Hart G.W.
    J. Neurochem. 79:1080-1089(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility."
    Liu Y., Fallon L., Lashuel H.A., Liu Z., Lansbury P.T. Jr.
    Cell 111:209-218(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNCA.

Entry informationi

Entry nameiUCHL1_RAT
AccessioniPrimary (citable) accession number: Q00981
Secondary accession number(s): Q6P9V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 5, 2005
Last modified: March 4, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.