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Q00978

- IRF9_HUMAN

UniProt

Q00978 - IRF9_HUMAN

Protein

Interferon regulatory factor 9

Gene

IRF9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi9 – 116108IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. regulatory region DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: ProtInc
    2. cytokine-mediated signaling pathway Source: Reactome
    3. defense response to virus Source: UniProtKB-KW
    4. interferon-gamma-mediated signaling pathway Source: Reactome
    5. transcription from RNA polymerase II promoter Source: ProtInc
    6. type I interferon biosynthetic process Source: Ensembl
    7. type I interferon signaling pathway Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Antiviral defense, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_25078. Interferon gamma signaling.
    REACT_25162. Interferon alpha/beta signaling.
    SignaLinkiQ00978.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon regulatory factor 9
    Short name:
    IRF-9
    Alternative name(s):
    IFN-alpha-responsive transcription factor subunit
    ISGF3 p48 subunit
    Interferon-stimulated gene factor 3 gamma
    Short name:
    ISGF-3 gamma
    Transcriptional regulator ISGF3 subunit gamma
    Gene namesi
    Name:IRF9
    Synonyms:ISGF3G
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:6131. IRF9.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Translocated into the nucleus upon activation by IFN-alpha/beta.

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: Reactome
    3. nucleoplasm Source: Reactome
    4. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162392259.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Interferon regulatory factor 9PRO_0000154567Add
    BLAST

    Proteomic databases

    MaxQBiQ00978.
    PaxDbiQ00978.
    PRIDEiQ00978.

    PTM databases

    PhosphoSiteiQ00978.

    Expressioni

    Inductioni

    By IFN-alpha and IFNB1/IFN-beta.

    Gene expression databases

    BgeeiQ00978.
    CleanExiHS_IRF9.
    GenevestigatoriQ00978.

    Organism-specific databases

    HPAiHPA001862.

    Interactioni

    Subunit structurei

    Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IFNAR2P485516EBI-626526,EBI-958408
    STAT2P526306EBI-626526,EBI-1546963

    Protein-protein interaction databases

    BioGridi115652. 22 interactions.
    IntActiQ00978. 6 interactions.
    MINTiMINT-1526689.
    STRINGi9606.ENSP00000380073.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00978.
    SMRiQ00978. Positions 4-116, 253-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi181 – 1877Poly-Ser

    Sequence similaritiesi

    Belongs to the IRF family.PROSITE-ProRule annotation
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG43166.
    HOGENOMiHOG000010107.
    HOVERGENiHBG003072.
    InParanoidiQ00978.
    KOiK04693.
    OMAiRCTRKLR.
    OrthoDBiEOG7CCBR1.
    PhylomeDBiQ00978.
    TreeFamiTF328512.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProiIPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view]
    PRINTSiPR00267. INTFRNREGFCT.
    SMARTiSM00348. IRF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00978-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF    50
    REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER 100
    GRMDVAEPYK VYQLLPPGIV SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ 150
    NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG SSSSSSSPEP QEVTDTTEAP 200
    FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD CRLVAEPSGS 250
    ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW 300
    NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN 350
    FWEESHGSSH TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV 393
    Length:393
    Mass (Da):43,696
    Last modified:April 1, 1993 - v1
    Checksum:iF8E3784354BFD4A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87503 mRNA. Translation: AAA58687.1.
    CH471078 Genomic DNA. Translation: EAW66093.1.
    CH471078 Genomic DNA. Translation: EAW66094.1.
    BC035716 mRNA. Translation: AAH35716.1.
    CCDSiCCDS9615.1.
    PIRiA45017.
    RefSeqiNP_006075.3. NM_006084.4.
    UniGeneiHs.1706.

    Genome annotation databases

    EnsembliENST00000396864; ENSP00000380073; ENSG00000213928.
    GeneIDi10379.
    KEGGihsa:10379.
    UCSCiuc001wmq.3. human.

    Polymorphism databases

    DMDMi266392.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87503 mRNA. Translation: AAA58687.1 .
    CH471078 Genomic DNA. Translation: EAW66093.1 .
    CH471078 Genomic DNA. Translation: EAW66094.1 .
    BC035716 mRNA. Translation: AAH35716.1 .
    CCDSi CCDS9615.1.
    PIRi A45017.
    RefSeqi NP_006075.3. NM_006084.4.
    UniGenei Hs.1706.

    3D structure databases

    ProteinModelPortali Q00978.
    SMRi Q00978. Positions 4-116, 253-380.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115652. 22 interactions.
    IntActi Q00978. 6 interactions.
    MINTi MINT-1526689.
    STRINGi 9606.ENSP00000380073.

    PTM databases

    PhosphoSitei Q00978.

    Polymorphism databases

    DMDMi 266392.

    Proteomic databases

    MaxQBi Q00978.
    PaxDbi Q00978.
    PRIDEi Q00978.

    Protocols and materials databases

    DNASUi 10379.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396864 ; ENSP00000380073 ; ENSG00000213928 .
    GeneIDi 10379.
    KEGGi hsa:10379.
    UCSCi uc001wmq.3. human.

    Organism-specific databases

    CTDi 10379.
    GeneCardsi GC14P024630.
    HGNCi HGNC:6131. IRF9.
    HPAi HPA001862.
    MIMi 147574. gene.
    neXtProti NX_Q00978.
    PharmGKBi PA162392259.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43166.
    HOGENOMi HOG000010107.
    HOVERGENi HBG003072.
    InParanoidi Q00978.
    KOi K04693.
    OMAi RCTRKLR.
    OrthoDBi EOG7CCBR1.
    PhylomeDBi Q00978.
    TreeFami TF328512.

    Enzyme and pathway databases

    Reactomei REACT_25078. Interferon gamma signaling.
    REACT_25162. Interferon alpha/beta signaling.
    SignaLinki Q00978.

    Miscellaneous databases

    GeneWikii IRF9.
    ISGF3G.
    GenomeRNAii 10379.
    NextBioi 39319.
    PROi Q00978.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q00978.
    CleanExi HS_IRF9.
    Genevestigatori Q00978.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProi IPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view ]
    PRINTSi PR00267. INTFRNREGFCT.
    SMARTi SM00348. IRF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins."
      Veals S.A., Schindler C., Leonard D.G.B., Fu X.-Y., Aebersold R.H., Darnell J.E. Jr., Levy D.E.
      Mol. Cell. Biol. 12:3315-3324(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    4. "Nuclear/cytoplasmic localization of IRFs in response to viral infection or interferon stimulation."
      Reich N.C.
      J. Interferon Cytokine Res. 22:103-109(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Viral inhibition of interferon signal transduction."
      Cebulla C.M., Miller D.M., Sedmak D.D.
      Intervirology 42:325-330(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiIRF9_HUMAN
    AccessioniPrimary (citable) accession number: Q00978
    Secondary accession number(s): D3DS61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3