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Protein

Interferon regulatory factor 9

Gene

IRF9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi9 – 116IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST108

GO - Molecular functioni

  • core promoter binding Source: UniProtKB
  • DNA binding transcription factor activity Source: ProtInc
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • defense response to virus Source: UniProtKB-KW
  • interferon-gamma-mediated signaling pathway Source: Reactome
  • transcription by RNA polymerase II Source: ProtInc
  • type I interferon signaling pathway Source: Reactome

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAntiviral defense, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling
R-HSA-909733 Interferon alpha/beta signaling
SignaLinkiQ00978
SIGNORiQ00978

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 9
Short name:
IRF-9
Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name:
ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma
Gene namesi
Name:IRF9
Synonyms:ISGF3G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000213928.8
HGNCiHGNC:6131 IRF9
MIMi147574 gene
neXtProtiNX_Q00978

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10379
OpenTargetsiENSG00000213928
PharmGKBiPA162392259

Polymorphism and mutation databases

BioMutaiIRF9
DMDMi266392

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545671 – 393Interferon regulatory factor 9Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ00978
PaxDbiQ00978
PeptideAtlasiQ00978
PRIDEiQ00978

PTM databases

iPTMnetiQ00978
PhosphoSitePlusiQ00978

Expressioni

Inductioni

By IFN-alpha and IFNB1/IFN-beta.

Gene expression databases

BgeeiENSG00000213928
CleanExiHS_IRF9
ExpressionAtlasiQ00978 baseline and differential
GenevisibleiQ00978 HS

Organism-specific databases

HPAiHPA001862

Interactioni

Subunit structurei

Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115652, 31 interactors
CORUMiQ00978
IntActiQ00978, 7 interactors
MINTiQ00978
STRINGi9606.ENSP00000380073

Structurei

3D structure databases

ProteinModelPortaliQ00978
SMRiQ00978
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 187Poly-Ser7

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IECM Eukaryota
ENOG4111AEC LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000010107
HOVERGENiHBG003072
InParanoidiQ00978
KOiK04693
OMAiRCTRKLR
OrthoDBiEOG091G067P
PhylomeDBiQ00978
TreeFamiTF328512

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
2.60.200.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR019471 Interferon_reg_factor-3
IPR017855 SMAD-like_dom_sf
IPR008984 SMAD_FHA_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PF10401 IRF-3, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SM01243 IRF-3, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF49879 SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q00978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF
60 70 80 90 100
REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER
110 120 130 140 150
GRMDVAEPYK VYQLLPPGIV SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ
160 170 180 190 200
NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG SSSSSSSPEP QEVTDTTEAP
210 220 230 240 250
FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD CRLVAEPSGS
260 270 280 290 300
ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW
310 320 330 340 350
NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN
360 370 380 390
FWEESHGSSH TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV
Length:393
Mass (Da):43,696
Last modified:April 1, 1993 - v1
Checksum:iF8E3784354BFD4A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87503 mRNA Translation: AAA58687.1
CH471078 Genomic DNA Translation: EAW66093.1
CH471078 Genomic DNA Translation: EAW66094.1
BC035716 mRNA Translation: AAH35716.1
CCDSiCCDS9615.1
PIRiA45017
RefSeqiNP_006075.3, NM_006084.4
UniGeneiHs.1706

Genome annotation databases

EnsembliENST00000396864; ENSP00000380073; ENSG00000213928
GeneIDi10379
KEGGihsa:10379
UCSCiuc058zzb.1 human

Similar proteinsi

Entry informationi

Entry nameiIRF9_HUMAN
AccessioniPrimary (citable) accession number: Q00978
Secondary accession number(s): D3DS61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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