Q00978 (IRF9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon regulatory factor 9
Short name=IRF-9

Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name=ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma

Gene names

Name:	IRF9
Synonyms:	ISGF3G

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Subunit structure	Interacts with STAT2 in the cytoplasm. Forms a complex with heterodimer STAT1/STAT2.
Subcellular location	Cytoplasm. Nucleus. Note: Translocated into the nucleus upon activation by IFN-alpha/beta. Ref.4
Induction	By IFN-alpha and IFNB1/IFN-beta.
Sequence similarities	Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Ontologies

Keywords
Biological process	Antiviral defense Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome response to virus Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: IntAct regulatory region DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IFNAR2	P48551	6	EBI-626526,EBI-958408
STAT2	P52630	3	EBI-626526,EBI-1546963

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Interferon regulatory factor 9		PRO_0000154567
Regions
	DNA binding	9 – 116	108	IRF tryptophan pentad repeat
	Compositional bias	181 – 187	7	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q00978 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F8E3784354BFD4A9	FASTA	393	43,696
	10 20 30 40 50 60 MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF REDQDAAFFK 70 80 90 100 110 120 AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER GRMDVAEPYK VYQLLPPGIV 130 140 150 160 170 180 SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG 190 200 210 220 230 240 SSSSSSSPEP QEVTDTTEAP FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD 250 260 270 280 290 300 CRLVAEPSGS ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW 310 320 330 340 350 360 NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN FWEESHGSSH 370 380 390 TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins." Veals S.A., Schindler C., Leonard D.G.B., Fu X.-Y., Aebersold R.H., Darnell J.E. Jr., Levy D.E. Mol. Cell. Biol. 12:3315-3324(1992) [PubMed: 1630447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Nuclear/cytoplasmic localization of IRFs in response to viral infection or interferon stimulation." Reich N.C. J. Interferon Cytokine Res. 22:103-109(2002) [PubMed: 11846981] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Viral inhibition of interferon signal transduction." Cebulla C.M., Miller D.M., Sedmak D.D. Intervirology 42:325-330(1999) [PubMed: 10702714] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M87503 mRNA. Translation: AAA58687.1. CH471078 Genomic DNA. Translation: EAW66093.1. CH471078 Genomic DNA. Translation: EAW66094.1. BC035716 mRNA. Translation: AAH35716.1.
IPI	IPI00025478.
PIR	A45017.
RefSeq	NP_006075.3. NM_006084.4.
UniGene	Hs.1706.
3D structure databases
ProteinModelPortal	Q00978.
SMR	Q00978. Positions 4-120, 215-393.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00978. 8 interactions.
MINT	MINT-1526689.
STRING	Q00978.
PTM databases
PhosphoSite	Q00978.
Polymorphism databases
DMDM	266392.
Proteomic databases
PRIDE	Q00978.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000396864; ENSP00000380073; ENSG00000213928.
GeneID	10379.
KEGG	hsa:10379.
UCSC	uc001wmq.1. human.
Organism-specific databases
CTD	10379.
GeneCards	GC14P024630.
H-InvDB	HIX0079551. HIX0079610.
HGNC	HGNC:6131. IRF9.
HPA	HPA001862.
MIM	147574. gene.
neXtProt	NX_Q00978.
PharmGKB	PA162392259.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074205.
HOGENOM	HBG445888.
HOVERGEN	HBG003072.
InParanoid	Q00978.
OMA	RCTRKLR.
OrthoDB	EOG42FSHX.
Enzyme and pathway databases
Pathway_Interaction_DB	ifngpathway. IFN-gamma pathway.
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q00978.
Bgee	Q00978.
CleanEx	HS_IRF9.
Genevestigator	Q00978.
Family and domain databases
InterPro	IPR019817. Interferon_reg_fac_CS. IPR001346. Interferon_reg_fact_DNA-bd_dom. IPR019471. Interferon_reg_factor-3. IPR017855. SMAD_dom-like. IPR008984. SMAD_FHA_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.60.200.10. MH2_Dwarfin-type. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K04693.
Pfam	PF00605. IRF. 1 hit. PF10401. IRF-3. 1 hit. [Graphical view]
PRINTS	PR00267. INTFRNREGFCT.
SMART	SM00348. IRF. 1 hit. [Graphical view]
SUPFAM	SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS00601. IRF_1. 1 hit. PS51507. IRF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39319.
SOURCE	Search...

Entry information

Entry name

IRF9_HUMAN

Accession

Primary (citable) accession number: Q00978
Secondary accession number(s): D3DS61

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.