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Protein

Interferon regulatory factor 9

Gene

IRF9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi9 – 116IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST108

GO - Molecular functioni

  • core promoter binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • defense response to virus Source: UniProtKB-KW
  • interferon-gamma-mediated signaling pathway Source: Reactome
  • transcription from RNA polymerase II promoter Source: ProtInc
  • type I interferon signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Antiviral defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-31791-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.
R-HSA-909733. Interferon alpha/beta signaling.
SignaLinkiQ00978.
SIGNORiQ00978.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 9
Short name:
IRF-9
Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name:
ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma
Gene namesi
Name:IRF9
Synonyms:ISGF3G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:6131. IRF9.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Translocated into the nucleus upon activation by IFN-alpha/beta.

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • nucleoplasm Source: Reactome
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10379.
OpenTargetsiENSG00000213928.
PharmGKBiPA162392259.

Polymorphism and mutation databases

BioMutaiIRF9.
DMDMi266392.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545671 – 393Interferon regulatory factor 9Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ00978.
PaxDbiQ00978.
PeptideAtlasiQ00978.
PRIDEiQ00978.

PTM databases

iPTMnetiQ00978.
PhosphoSitePlusiQ00978.

Expressioni

Inductioni

By IFN-alpha and IFNB1/IFN-beta.

Gene expression databases

BgeeiENSG00000213928.
CleanExiHS_IRF9.
ExpressionAtlasiQ00978. baseline and differential.
GenevisibleiQ00978. HS.

Organism-specific databases

HPAiHPA001862.

Interactioni

Subunit structurei

Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.

Binary interactionsi

WithEntry#Exp.IntActNotes
IFNAR2P485516EBI-626526,EBI-958408
STAT2P526306EBI-626526,EBI-1546963

Protein-protein interaction databases

BioGridi115652. 30 interactors.
IntActiQ00978. 7 interactors.
MINTiMINT-1526689.
STRINGi9606.ENSP00000380073.

Structurei

3D structure databases

ProteinModelPortaliQ00978.
SMRiQ00978.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 187Poly-Ser7

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IECM. Eukaryota.
ENOG4111AEC. LUCA.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ00978.
KOiK04693.
OMAiRCTRKLR.
OrthoDBiEOG091G067P.
PhylomeDBiQ00978.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF
60 70 80 90 100
REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER
110 120 130 140 150
GRMDVAEPYK VYQLLPPGIV SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ
160 170 180 190 200
NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG SSSSSSSPEP QEVTDTTEAP
210 220 230 240 250
FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD CRLVAEPSGS
260 270 280 290 300
ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW
310 320 330 340 350
NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN
360 370 380 390
FWEESHGSSH TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV
Length:393
Mass (Da):43,696
Last modified:April 1, 1993 - v1
Checksum:iF8E3784354BFD4A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87503 mRNA. Translation: AAA58687.1.
CH471078 Genomic DNA. Translation: EAW66093.1.
CH471078 Genomic DNA. Translation: EAW66094.1.
BC035716 mRNA. Translation: AAH35716.1.
CCDSiCCDS9615.1.
PIRiA45017.
RefSeqiNP_006075.3. NM_006084.4.
UniGeneiHs.1706.

Genome annotation databases

EnsembliENST00000396864; ENSP00000380073; ENSG00000213928.
GeneIDi10379.
KEGGihsa:10379.
UCSCiuc058zzb.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87503 mRNA. Translation: AAA58687.1.
CH471078 Genomic DNA. Translation: EAW66093.1.
CH471078 Genomic DNA. Translation: EAW66094.1.
BC035716 mRNA. Translation: AAH35716.1.
CCDSiCCDS9615.1.
PIRiA45017.
RefSeqiNP_006075.3. NM_006084.4.
UniGeneiHs.1706.

3D structure databases

ProteinModelPortaliQ00978.
SMRiQ00978.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115652. 30 interactors.
IntActiQ00978. 7 interactors.
MINTiMINT-1526689.
STRINGi9606.ENSP00000380073.

PTM databases

iPTMnetiQ00978.
PhosphoSitePlusiQ00978.

Polymorphism and mutation databases

BioMutaiIRF9.
DMDMi266392.

Proteomic databases

MaxQBiQ00978.
PaxDbiQ00978.
PeptideAtlasiQ00978.
PRIDEiQ00978.

Protocols and materials databases

DNASUi10379.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396864; ENSP00000380073; ENSG00000213928.
GeneIDi10379.
KEGGihsa:10379.
UCSCiuc058zzb.1. human.

Organism-specific databases

CTDi10379.
DisGeNETi10379.
GeneCardsiIRF9.
HGNCiHGNC:6131. IRF9.
HPAiHPA001862.
MIMi147574. gene.
neXtProtiNX_Q00978.
OpenTargetsiENSG00000213928.
PharmGKBiPA162392259.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IECM. Eukaryota.
ENOG4111AEC. LUCA.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ00978.
KOiK04693.
OMAiRCTRKLR.
OrthoDBiEOG091G067P.
PhylomeDBiQ00978.
TreeFamiTF328512.

Enzyme and pathway databases

BioCyciZFISH:G66-31791-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.
R-HSA-909733. Interferon alpha/beta signaling.
SignaLinkiQ00978.
SIGNORiQ00978.

Miscellaneous databases

GeneWikiiIRF9.
ISGF3G.
GenomeRNAii10379.
PROiQ00978.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000213928.
CleanExiHS_IRF9.
ExpressionAtlasiQ00978. baseline and differential.
GenevisibleiQ00978. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIRF9_HUMAN
AccessioniPrimary (citable) accession number: Q00978
Secondary accession number(s): D3DS61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.