Reviewed, UniProtKB/Swiss-Prot Q00978 (IRF9_HUMAN)

Names and origin

Protein names

Recommended name:
    Interferon regulatory factor 9
      Short name=IRF-9
Alternative name(s):
    Transcriptional regulator ISGF3 subunit gamma
    IFN-alpha-responsive transcription factor subunit
    Interferon-stimulated gene factor 3 gamma
    ISGF3 p48 subunit
    ISGF-3 gamma

Gene names

Name:	IRF9
Synonyms:	ISGF3G

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Subunit structure	Interacts with STAT2 in the cytoplasm. Forms a complex with heterodimer STAT1/STAT2.
Subcellular location	Cytoplasm. Nucleus. Note: Translocated into the nucleus upon activation by IFN-alpha/beta. Ref.3
Induction	By IFN-alpha and IFN-beta.
Sequence similarities	Belongs to the IRF family. Contains 1 tryptophan pentad repeat DNA-binding domain.

Ontologies

Keywords
Biological process	Antiviral defense Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Ref.1 Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to virus Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: HPA nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-626526,EBI-626526
IFNAR2	P48551	4	EBI-626526,EBI-958408
STAT2	P52630	3	EBI-626526,EBI-1546963

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Interferon regulatory factor 9		PRO_0000154567
Regions
	DNA binding	11 – 112	102	Tryptophan pentad repeat
	Compositional bias	181 – 187	7	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q00978-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F8E3784354BFD4A9	FASTA	393	43,696
	10 20 30 40 50 60 MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF REDQDAAFFK 70 80 90 100 110 120 AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER GRMDVAEPYK VYQLLPPGIV 130 140 150 160 170 180 SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG 190 200 210 220 230 240 SSSSSSSPEP QEVTDTTEAP FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD 250 260 270 280 290 300 CRLVAEPSGS ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW 310 320 330 340 350 360 NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN FWEESHGSSH 370 380 390 TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins." Veals S.A., Schindler C., Leonard D.G.B., Fu X.-Y., Aebersold R.H., Darnell J.E. Jr., Levy D.E. Mol. Cell. Biol. 12:3315-3324(1992) [PubMed: 1630447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	"Nuclear/cytoplasmic localization of IRFs in response to viral infection or interferon stimulation." Reich N.C. J. Interferon Cytokine Res. 22:103-109(2002) [PubMed: 11846981] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Viral inhibition of interferon signal transduction." Cebulla C.M., Miller D.M., Sedmak D.D. Intervirology 42:325-330(1999) [PubMed: 10702714] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87503 mRNA. Translation: AAA58687.1. BC035716 mRNA. Translation: AAH35716.1.
IPI	IPI00025478.
PIR	A45017.
RefSeq	NP_006075.3.
UniGene	Hs.1706
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00978. 4 interactions.
Genome annotation databases
Ensembl	ENST00000396864; ENSP00000380073; ENSG00000213928; Homo sapiens. [Genome view]
GeneID	10379.
KEGG	hsa:10379.
UCSC	uc001wmq.1. human.
Organism-specific databases
CTD	10379.
GeneCards	GC14P023690.
H-InvDB	HIX0079551. HIX0079610.
HGNC	HGNC:6131. IRF9.
HPA	HPA001862.
MIM	147574. gene.
PharmGKB	PA29931.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q00978.
OMA	LFVQRLC
OrthoDB	EOG90KBJX
Enzyme and pathway databases
Pathway_Interaction_DB	ifngpathway. IFN-gamma pathway.
Gene expression databases
ArrayExpress	Q00978.
Bgee	Q00978.
CleanEx	HS_IRF9.
Genevestigator	Q00978.
Family and domain databases
InterPro	IPR019817. Interferon_regulatory_fac_CS. IPR001346. Interferon_regulatory_factor. IPR019471. Interferon_regulatory_factor-3. IPR017855. SMAD_dom-like. IPR008984. SMAD_FHA_domain. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.60.200.10. MH2_Dwarfin-type. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00605. IRF. 1 hit. PF10401. IRF-3. 1 hit. [Graphical view]
PRINTS	PR00267. INTFRNREGFCT.
SMART	SM00348. IRF. 1 hit. [Graphical view]
PROSITE	PS00601. IRF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39319.
SOURCE	Search...

Entry information

Entry name

IRF9_HUMAN

Accession

Primary (citable) accession number: Q00978

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 24, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.