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Q00978 (IRF9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon regulatory factor 9

Short name=IRF-9
Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name=ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma
Gene names
Name:IRF9
Synonyms:ISGF3G
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Subunit structure

Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus upon activation by IFN-alpha/beta. Ref.4

Induction

By IFN-alpha and IFNB1/IFN-beta.

Sequence similarities

Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IFNAR2P485516EBI-626526,EBI-958408
STAT2P526304EBI-626526,EBI-1546963

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Interferon regulatory factor 9
PRO_0000154567

Regions

DNA binding9 – 116108IRF tryptophan pentad repeat
Compositional bias181 – 1877Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
Q00978 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: F8E3784354BFD4A9

FASTA39343,696
        10         20         30         40         50         60 
MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF REDQDAAFFK 

        70         80         90        100        110        120 
AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER GRMDVAEPYK VYQLLPPGIV 

       130        140        150        160        170        180 
SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG 

       190        200        210        220        230        240 
SSSSSSSPEP QEVTDTTEAP FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD 

       250        260        270        280        290        300 
CRLVAEPSGS ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW 

       310        320        330        340        350        360 
NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN FWEESHGSSH 

       370        380        390 
TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV 

« Hide

References

« Hide 'large scale' references
[1]"Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins."
Veals S.A., Schindler C., Leonard D.G.B., Fu X.-Y., Aebersold R.H., Darnell J.E. Jr., Levy D.E.
Mol. Cell. Biol. 12:3315-3324(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[4]"Nuclear/cytoplasmic localization of IRFs in response to viral infection or interferon stimulation."
Reich N.C.
J. Interferon Cytokine Res. 22:103-109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Viral inhibition of interferon signal transduction."
Cebulla C.M., Miller D.M., Sedmak D.D.
Intervirology 42:325-330(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M87503 mRNA. Translation: AAA58687.1.
CH471078 Genomic DNA. Translation: EAW66093.1.
CH471078 Genomic DNA. Translation: EAW66094.1.
BC035716 mRNA. Translation: AAH35716.1.
PIRA45017.
RefSeqNP_006075.3. NM_006084.4.
UniGeneHs.1706.

3D structure databases

ProteinModelPortalQ00978.
SMRQ00978. Positions 4-116, 209-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115652. 22 interactions.
IntActQ00978. 6 interactions.
MINTMINT-1526689.
STRING9606.ENSP00000380073.

PTM databases

PhosphoSiteQ00978.

Polymorphism databases

DMDM266392.

Proteomic databases

PaxDbQ00978.
PRIDEQ00978.

Protocols and materials databases

DNASU10379.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000396864; ENSP00000380073; ENSG00000213928.
GeneID10379.
KEGGhsa:10379.
UCSCuc001wmq.3. human.

Organism-specific databases

CTD10379.
GeneCardsGC14P024630.
HGNCHGNC:6131. IRF9.
HPAHPA001862.
MIM147574. gene.
neXtProtNX_Q00978.
PharmGKBPA162392259.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43166.
HOGENOMHOG000010107.
HOVERGENHBG003072.
InParanoidQ00978.
KOK04693.
OMARCTRKLR.
OrthoDBEOG7CCBR1.
PhylomeDBQ00978.
TreeFamTF328512.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkQ00978.

Gene expression databases

BgeeQ00978.
CleanExHS_IRF9.
GenevestigatorQ00978.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSPR00267. INTFRNREGFCT.
SMARTSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiIRF9.
ISGF3G.
GenomeRNAi10379.
NextBio39319.
PROQ00978.
SOURCESearch...

Entry information

Entry nameIRF9_HUMAN
AccessionPrimary (citable) accession number: Q00978
Secondary accession number(s): D3DS61
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM