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Q00977 (CXB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gap junction beta-2 protein
Alternative name(s):
Connexin-26
Short name=Cx26
Gene names
Name:Gjb2
Synonyms:Cxn-26
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subunit structure

A connexon is composed of a hexamer of connexins. Interacts with CNST. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctiongap junction.

Tissue specificity

Liver, kidney, intestine, lung, spleen, stomach, testis and brain, but not heart and adult skeletal muscle.

Sequence similarities

Belongs to the connexin family. Beta-type (group I) subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226Gap junction beta-2 protein
PRO_0000057858

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4020Helical; Potential
Topological domain41 – 7535Extracellular Potential
Transmembrane76 – 9823Helical; Potential
Topological domain99 – 13941Cytoplasmic Potential
Transmembrane140 – 16223Helical; Potential
Topological domain163 – 19230Extracellular Potential
Transmembrane193 – 21523Helical; Potential
Topological domain216 – 22611Cytoplasmic Potential

Amino acid modifications

Disulfide bond53 ↔ 180 By similarity
Disulfide bond60 ↔ 174 By similarity
Disulfide bond64 ↔ 169 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00977 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 0D8178F7A339E0B6

FASTA22626,411
        10         20         30         40         50         60 
MDWGTLQSIL GGVNKHSTSI GKIWLTVLFI FRIMILVVAA KEVWGDEQAD FVCNTLQPGC 

        70         80         90        100        110        120 
KNVCYDHHFP ISHIRLWALQ LIMVSTPALL VAMHVAYRRH EKKRKFMKGE IKNEFKDIEE 

       130        140        150        160        170        180 
IKTQKVRIEG SLWWTYTTSI FFRVIFEAVF MYVFYIMYNG FFMQRLVKCN AWPCPNTVDC 

       190        200        210        220 
FISRPTEKTV FTVFMISVSG ICILLNITEL CYLFVRYCSG KSKRPV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes."
Willecke K., Nicholson B.J., Dahl E., Kozjek G., Hennemann H.
Eur. J. Cell Biol. 58:81-89(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Developmental regulation of gap junction gene expression during mouse embryonic development."
Nishi M., Kumar N.M., Gilula N.B.
Dev. Biol. 146:117-130(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver and Olfactory epithelium.
[4]"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins."
del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C.
Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CNST.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M81445 Genomic DNA. Translation: AAA37495.1.
M63803 mRNA. Translation: AAA37276.1.
BC013634 mRNA. Translation: AAH13634.1.
BC051437 mRNA. Translation: AAH51437.1.
CCDSCCDS27153.1.
RefSeqNP_032151.1. NM_008125.3.
UniGeneMm.390683.

3D structure databases

ProteinModelPortalQ00977.
SMRQ00977. Positions 2-217.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00977. 1 interaction.

PTM databases

PhosphoSiteQ00977.

Proteomic databases

MaxQBQ00977.
PaxDbQ00977.
PRIDEQ00977.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055698; ENSMUSP00000054343; ENSMUSG00000046352.
GeneID14619.
KEGGmmu:14619.
UCSCuc007ucx.2. mouse.

Organism-specific databases

CTD2706.
MGIMGI:95720. Gjb2.

Phylogenomic databases

eggNOGNOG39645.
HOGENOMHOG000231127.
HOVERGENHBG009576.
InParanoidQ00977.
KOK07621.
OMAMYVFYIM.
OrthoDBEOG7P2XSS.
PhylomeDBQ00977.
TreeFamTF329606.

Gene expression databases

ArrayExpressQ00977.
BgeeQ00977.
CleanExMM_GJB2.
GenevestigatorQ00977.

Family and domain databases

InterProIPR000500. Connexin.
IPR002268. Connexin26.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERPTHR11984. PTHR11984. 1 hit.
PfamPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSPR00206. CONNEXIN.
PR01139. CONNEXINB2.
SMARTSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286440.
PROQ00977.
SOURCESearch...

Entry information

Entry nameCXB2_MOUSE
AccessionPrimary (citable) accession number: Q00977
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot