Q00977 (CXB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction beta-2 protein

Alternative name(s):
Connexin-26
Short name=Cx26

Gene names

Name:	Gjb2
Synonyms:	Cxn-26

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins. Interacts with CNST. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Liver, kidney, intestine, lung, spleen, stomach, testis and brain, but not heart and adult skeletal muscle.
Sequence similarities	Belongs to the connexin family. Beta-type (group I) subfamily.

Ontologies

Keywords
Biological process	Hearing
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Inferred from direct assay PubMed 11501764. Source: MGI male genitalia development Inferred from electronic annotation. Source: Compara sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	connexon complex Inferred from electronic annotation. Source: InterPro gap junction Inferred from direct assay PubMed 11501764PubMed 14595769PubMed 20089884. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lateral plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	gap junction channel activity Inferred from direct assay PubMed 11501764. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Gap junction beta-2 protein		PRO_0000057858
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 40	20	Helical; Potential
	Topological domain	41 – 75	35	Extracellular Potential
	Transmembrane	76 – 98	23	Helical; Potential
	Topological domain	99 – 139	41	Cytoplasmic Potential
	Transmembrane	140 – 162	23	Helical; Potential
	Topological domain	163 – 192	30	Extracellular Potential
	Transmembrane	193 – 215	23	Helical; Potential
	Topological domain	216 – 226	11	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	53 ↔ 180		By similarity
	Disulfide bond	60 ↔ 174		By similarity
	Disulfide bond	64 ↔ 169		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00977 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0D8178F7A339E0B6	FASTA	226	26,411
	10 20 30 40 50 60 MDWGTLQSIL GGVNKHSTSI GKIWLTVLFI FRIMILVVAA KEVWGDEQAD FVCNTLQPGC 70 80 90 100 110 120 KNVCYDHHFP ISHIRLWALQ LIMVSTPALL VAMHVAYRRH EKKRKFMKGE IKNEFKDIEE 130 140 150 160 170 180 IKTQKVRIEG SLWWTYTTSI FFRVIFEAVF MYVFYIMYNG FFMQRLVKCN AWPCPNTVDC 190 200 210 220 FISRPTEKTV FTVFMISVSG ICILLNITEL CYLFVRYCSG KSKRPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes." Willecke K., Nicholson B.J., Dahl E., Kozjek G., Hennemann H. Eur. J. Cell Biol. 58:81-89(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Developmental regulation of gap junction gene expression during mouse embryonic development." Nishi M., Kumar N.M., Gilula N.B. Dev. Biol. 146:117-130(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Olfactory epithelium.
[4]	"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins." del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C. Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CNST.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81445 Genomic DNA. Translation: AAA37495.1. M63803 mRNA. Translation: AAA37276.1. BC013634 mRNA. Translation: AAH13634.1. BC051437 mRNA. Translation: AAH51437.1.
IPI	IPI00125162.
RefSeq	NP_032151.1. NM_008125.3.
UniGene	Mm.390683.
3D structure databases
ProteinModelPortal	Q00977.
SMR	Q00977. Positions 2-217.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00977. 1 interaction.
PTM databases
PhosphoSite	Q00977.
Proteomic databases
PaxDb	Q00977.
PRIDE	Q00977.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000055698; ENSMUSP00000054343; ENSMUSG00000046352.
GeneID	14619.
KEGG	mmu:14619.
Organism-specific databases
CTD	2706.
MGI	MGI:95720. Gjb2.
Phylogenomic databases
eggNOG	NOG39645.
HOGENOM	HOG000231127.
HOVERGEN	HBG009576.
InParanoid	Q00977.
KO	K07621.
OMA	MDWSTLQ.
OrthoDB	EOG4PK28F.
Gene expression databases
ArrayExpress	Q00977.
Bgee	Q00977.
CleanEx	MM_GJB2.
Genevestigator	Q00977.
GermOnline	ENSMUSG00000046352. Mus musculus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002268. Connexin26. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit. PTHR11984:SF24. PTHR11984:SF24. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01139. CONNEXINB2.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286440.
SOURCE	Search...

Entry information

Entry name

CXB2_MOUSE

Accession

Primary (citable) accession number: Q00977

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program