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Q00972 (BCKD_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

EC=2.7.11.4
Alternative name(s):
Branched-chain alpha-ketoacid dehydrogenase kinase
Short name=BCKD-kinase
Short name=BCKDHKIN
Gene names
Name:Bckdk
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Catalytic activity

ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.

Subunit structure

Monomer.

Subcellular location

Mitochondrion matrix.

Post-translational modification

Autophosphorylated.

Sequence similarities

Belongs to the PDK/BCKDK protein kinase family.

Contains 1 histidine kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3030Mitochondrion Ref.1
Chain31 – 412382[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
PRO_0000023454

Regions

Domain159 – 404246Histidine kinase

Amino acid modifications

Modified residue1921N6-acetyllysine By similarity
Modified residue2331N6-acetyllysine By similarity

Experimental info

Sequence conflict110 – 12415RSLPF…PTILH → VVFLSSLVATLPYCT Ref.1
Sequence conflict110 – 12415RSLPF…PTILH → VVFLSSLVATLPYCT Ref.2
Sequence conflict2221C → S Ref.1
Sequence conflict2221C → S Ref.2

Secondary structure

........................................... 412
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00972 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: D630D3A44728927E

FASTA41246,474
        10         20         30         40         50         60 
MILTSVLGSG PRSGSSLWPL LGSSLSLRVR STSATDTHHV ELARERSKTV TSFYNQSAID 

        70         80         90        100        110        120 
VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV RIAHRIKGFR SLPFIIGCNP 

       130        140        150        160        170        180 
TILHVHELYI RAFQKLTDFP PIKDQADEAQ YCQLVRQLLD DHKDVVTLLA EGLRESRKHI 

       190        200        210        220        230        240 
EDEKLVRYFL DKTLTSRLGI RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR 

       250        260        270        280        290        300 
LCEHKYGNAP RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI 

       310        320        330        340        350        360 
TIANNDVDLI IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG HLDMHSGGQS 

       370        380        390        400        410 
GPMHGFGFGL PTSRAYAEYL GGSLQLQSLQ GIGTDVYLRL RHIDGREESF RI 

« Hide

References

[1]"Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases."
Popov K.M., Zhao Y., Shimomura Y., Kuntz M.J., Harris R.A.
J. Biol. Chem. 267:13127-13130(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-67; 93-100; 254-260 AND 328-343.
Tissue: Heart.
[2]"Purification, characterization, regulation and molecular cloning of mitochondrial protein kinases."
Harris R.A., Popov K.M., Shimomura Y., Zhao Y., Jaskiewicz J., Nanaumi N., Suzuki M.
Adv. Enzyme Regul. 32:267-284(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[3]"Expression and characterization of branched-chain alpha-ketoacid dehydrogenase kinase from the rat. Is it a histidine-protein kinase?"
Davie J.R., Wynn R.M., Meng M., Huang Y.-S., Aalund G., Chuang D.T., Lau K.S.
J. Biol. Chem. 270:19861-19867(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-412, SEQUENCE REVISION TO 110-124 AND 222.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93271 mRNA. Translation: AAA40818.1.
U27456 mRNA. Translation: AAB60498.1.
PIRA42924.
RefSeqNP_062117.2. NM_019244.2.
UniGeneRn.31976.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GJVX-ray2.70A31-412[»]
1GKXX-ray2.30A31-412[»]
1GKZX-ray2.20A31-412[»]
3TZ0X-ray2.50A1-412[»]
3TZ2X-ray2.85A1-412[»]
3TZ4X-ray2.25A1-412[»]
3TZ5X-ray2.40A1-412[»]
3VADX-ray2.60A1-412[»]
4DZYX-ray2.10A1-412[»]
4E00X-ray2.15A1-412[»]
4E01X-ray1.97A1-412[»]
4E02X-ray2.15A1-412[»]
4H7QX-ray2.10A1-412[»]
4H81X-ray2.05A1-412[»]
4H85X-ray2.10A1-412[»]
ProteinModelPortalQ00972.
SMRQ00972. Positions 68-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248235. 1 interaction.
IntActQ00972. 1 interaction.
STRING10116.ENSRNOP00000026466.

PTM databases

PhosphoSiteQ00972.

Proteomic databases

PaxDbQ00972.
PRIDEQ00972.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026466; ENSRNOP00000026466; ENSRNOG00000019485.
GeneID29603.
KEGGrno:29603.
UCSCRGD:2198. rat.

Organism-specific databases

CTD10295.
RGD2198. Bckdk.

Phylogenomic databases

eggNOGCOG0642.
GeneTreeENSGT00550000074574.
HOGENOMHOG000164315.
HOVERGENHBG004829.
InParanoidQ00972.
KOK00905.
OMATIANNDV.
OrthoDBEOG7TQV0S.
PhylomeDBQ00972.
TreeFamTF331303.

Gene expression databases

GenevestigatorQ00972.

Family and domain databases

Gene3D1.20.140.20. 1 hit.
3.30.565.10. 1 hit.
InterProIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_ATP-bd.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
PfamPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSPR00344. BCTRLSENSOR.
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF55874. SSF55874. 1 hit.
SSF69012. SSF69012. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ00972.
NextBio609772.
PROQ00972.

Entry information

Entry nameBCKD_RAT
AccessionPrimary (citable) accession number: Q00972
Secondary accession number(s): Q64552
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references