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Protein

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Gene

Bckdk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.By similarity

Catalytic activityi

ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.

GO - Molecular functioni

  • [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity Source: RGD
  • ATP binding Source: HGNC
  • protein serine/threonine kinase activity Source: HGNC

GO - Biological processi

  • branched-chain amino acid catabolic process Source: HGNC
  • phosphorylation Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.4. 5301.
ReactomeiR-RNO-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial (EC:2.7.11.4)
Alternative name(s):
Branched-chain alpha-ketoacid dehydrogenase kinase
Short name:
BCKD-kinase
Short name:
BCKDHKIN
Gene namesi
Name:Bckdk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2198. Bckdk.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial alpha-ketoglutarate dehydrogenase complex Source: HGNC
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000002345431 – 412[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrialAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphoserineCombined sources1
Modified residuei192N6-acetyllysineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei356PhosphoserineCombined sources1
Modified residuei360PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ00972.
PRIDEiQ00972.

PTM databases

iPTMnetiQ00972.
PhosphoSitePlusiQ00972.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019485.
ExpressionAtlasiQ00972. baseline and differential.
GenevisibleiQ00972. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi248235. 1 interactor.
IntActiQ00972. 1 interactor.
STRINGi10116.ENSRNOP00000026466.

Structurei

Secondary structure

1412
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi69 – 73Combined sources5
Beta strandi74 – 76Combined sources3
Beta strandi82 – 84Combined sources3
Helixi85 – 111Combined sources27
Helixi114 – 117Combined sources4
Helixi120 – 138Combined sources19
Helixi145 – 161Combined sources17
Turni162 – 164Combined sources3
Helixi165 – 176Combined sources12
Helixi177 – 179Combined sources3
Helixi184 – 209Combined sources26
Beta strandi221 – 225Combined sources5
Helixi227 – 246Combined sources20
Beta strandi252 – 257Combined sources6
Beta strandi262 – 264Combined sources3
Helixi267 – 286Combined sources20
Beta strandi298 – 304Combined sources7
Beta strandi306 – 314Combined sources9
Helixi322 – 325Combined sources4
Turni326 – 329Combined sources4
Helixi369 – 379Combined sources11
Beta strandi383 – 389Combined sources7
Turni390 – 392Combined sources3
Beta strandi393 – 401Combined sources9
Beta strandi403 – 405Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GJVX-ray2.70A31-412[»]
1GKXX-ray2.30A31-412[»]
1GKZX-ray2.20A31-412[»]
3TZ0X-ray2.50A1-412[»]
3TZ2X-ray2.85A1-412[»]
3TZ4X-ray2.25A1-412[»]
3TZ5X-ray2.40A1-412[»]
3VADX-ray2.60A1-412[»]
4DZYX-ray2.10A1-412[»]
4E00X-ray2.15A1-412[»]
4E01X-ray1.97A1-412[»]
4E02X-ray2.15A1-412[»]
4H7QX-ray2.10A1-412[»]
4H81X-ray2.05A1-412[»]
4H85X-ray2.10A1-412[»]
ProteinModelPortaliQ00972.
SMRiQ00972.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00972.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 404Histidine kinasePROSITE-ProRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the PDK/BCKDK protein kinase family.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0787. Eukaryota.
COG0642. LUCA.
GeneTreeiENSGT00550000074574.
HOGENOMiHOG000164315.
HOVERGENiHBG004829.
InParanoidiQ00972.
KOiK00905.
OMAiQVHELYI.
PhylomeDBiQ00972.
TreeFamiTF331303.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
SSF69012. SSF69012. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILTSVLGSG PRSGSSLWPL LGSSLSLRVR STSATDTHHV ELARERSKTV
60 70 80 90 100
TSFYNQSAID VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV
110 120 130 140 150
RIAHRIKGFR SLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ
160 170 180 190 200
YCQLVRQLLD DHKDVVTLLA EGLRESRKHI EDEKLVRYFL DKTLTSRLGI
210 220 230 240 250
RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR LCEHKYGNAP
260 270 280 290 300
RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI
310 320 330 340 350
TIANNDVDLI IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG
360 370 380 390 400
HLDMHSGGQS GPMHGFGFGL PTSRAYAEYL GGSLQLQSLQ GIGTDVYLRL
410
RHIDGREESF RI
Length:412
Mass (Da):46,474
Last modified:July 15, 1998 - v2
Checksum:iD630D3A44728927E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 124RSLPF…PTILH → VVFLSSLVATLPYCT (PubMed:1377677).CuratedAdd BLAST15
Sequence conflicti110 – 124RSLPF…PTILH → VVFLSSLVATLPYCT (PubMed:1496922).CuratedAdd BLAST15
Sequence conflicti222C → S (PubMed:1377677).Curated1
Sequence conflicti222C → S (PubMed:1496922).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93271 mRNA. Translation: AAA40818.1.
U27456 mRNA. Translation: AAB60498.1.
PIRiA42924.
RefSeqiNP_062117.2. NM_019244.2.
UniGeneiRn.31976.

Genome annotation databases

EnsembliENSRNOT00000078578; ENSRNOP00000069014; ENSRNOG00000019485.
GeneIDi29603.
KEGGirno:29603.
UCSCiRGD:2198. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93271 mRNA. Translation: AAA40818.1.
U27456 mRNA. Translation: AAB60498.1.
PIRiA42924.
RefSeqiNP_062117.2. NM_019244.2.
UniGeneiRn.31976.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GJVX-ray2.70A31-412[»]
1GKXX-ray2.30A31-412[»]
1GKZX-ray2.20A31-412[»]
3TZ0X-ray2.50A1-412[»]
3TZ2X-ray2.85A1-412[»]
3TZ4X-ray2.25A1-412[»]
3TZ5X-ray2.40A1-412[»]
3VADX-ray2.60A1-412[»]
4DZYX-ray2.10A1-412[»]
4E00X-ray2.15A1-412[»]
4E01X-ray1.97A1-412[»]
4E02X-ray2.15A1-412[»]
4H7QX-ray2.10A1-412[»]
4H81X-ray2.05A1-412[»]
4H85X-ray2.10A1-412[»]
ProteinModelPortaliQ00972.
SMRiQ00972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248235. 1 interactor.
IntActiQ00972. 1 interactor.
STRINGi10116.ENSRNOP00000026466.

PTM databases

iPTMnetiQ00972.
PhosphoSitePlusiQ00972.

Proteomic databases

PaxDbiQ00972.
PRIDEiQ00972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000078578; ENSRNOP00000069014; ENSRNOG00000019485.
GeneIDi29603.
KEGGirno:29603.
UCSCiRGD:2198. rat.

Organism-specific databases

CTDi10295.
RGDi2198. Bckdk.

Phylogenomic databases

eggNOGiKOG0787. Eukaryota.
COG0642. LUCA.
GeneTreeiENSGT00550000074574.
HOGENOMiHOG000164315.
HOVERGENiHBG004829.
InParanoidiQ00972.
KOiK00905.
OMAiQVHELYI.
PhylomeDBiQ00972.
TreeFamiTF331303.

Enzyme and pathway databases

BRENDAi2.7.11.4. 5301.
ReactomeiR-RNO-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

EvolutionaryTraceiQ00972.
PROiQ00972.

Gene expression databases

BgeeiENSRNOG00000019485.
ExpressionAtlasiQ00972. baseline and differential.
GenevisibleiQ00972. RN.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
SSF69012. SSF69012. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCKD_RAT
AccessioniPrimary (citable) accession number: Q00972
Secondary accession number(s): Q64552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.