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Protein

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Gene

Bckdk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.By similarity

Catalytic activityi

ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.

GO - Molecular functioni

  • [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity Source: RGD
  • ATP binding Source: HGNC
  • protein serine/threonine kinase activity Source: HGNC

GO - Biological processi

  • branched-chain amino acid catabolic process Source: HGNC
  • phosphorylation Source: HGNC

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.4 5301
ReactomeiR-RNO-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial (EC:2.7.11.4)
Alternative name(s):
Branched-chain alpha-ketoacid dehydrogenase kinase
Short name:
BCKD-kinase
Short name:
BCKDHKIN
Gene namesi
Name:Bckdk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi2198 Bckdk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000002345431 – 412[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrialAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphoserineCombined sources1
Modified residuei192N6-acetyllysineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei356PhosphoserineCombined sources1
Modified residuei360PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ00972
PRIDEiQ00972

PTM databases

iPTMnetiQ00972
PhosphoSitePlusiQ00972

Expressioni

Gene expression databases

BgeeiENSRNOG00000019485
ExpressionAtlasiQ00972 baseline and differential
GenevisibleiQ00972 RN

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi248235, 1 interactor
IntActiQ00972, 1 interactor
STRINGi10116.ENSRNOP00000026466

Structurei

Secondary structure

1412
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi69 – 73Combined sources5
Beta strandi74 – 76Combined sources3
Beta strandi82 – 84Combined sources3
Helixi85 – 111Combined sources27
Helixi114 – 117Combined sources4
Helixi120 – 138Combined sources19
Helixi145 – 161Combined sources17
Turni162 – 164Combined sources3
Helixi165 – 176Combined sources12
Helixi177 – 179Combined sources3
Helixi184 – 209Combined sources26
Beta strandi221 – 225Combined sources5
Helixi227 – 246Combined sources20
Beta strandi252 – 257Combined sources6
Beta strandi262 – 264Combined sources3
Helixi267 – 286Combined sources20
Beta strandi298 – 304Combined sources7
Beta strandi306 – 314Combined sources9
Helixi322 – 325Combined sources4
Turni326 – 329Combined sources4
Helixi369 – 379Combined sources11
Beta strandi383 – 389Combined sources7
Turni390 – 392Combined sources3
Beta strandi393 – 401Combined sources9
Beta strandi403 – 405Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GJVX-ray2.70A31-412[»]
1GKXX-ray2.30A31-412[»]
1GKZX-ray2.20A31-412[»]
3TZ0X-ray2.50A1-412[»]
3TZ2X-ray2.85A1-412[»]
3TZ4X-ray2.25A1-412[»]
3TZ5X-ray2.40A1-412[»]
3VADX-ray2.60A1-412[»]
4DZYX-ray2.10A1-412[»]
4E00X-ray2.15A1-412[»]
4E01X-ray1.97A1-412[»]
4E02X-ray2.15A1-412[»]
4H7QX-ray2.10A1-412[»]
4H81X-ray2.05A1-412[»]
4H85X-ray2.10A1-412[»]
ProteinModelPortaliQ00972
SMRiQ00972
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00972

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 404Histidine kinasePROSITE-ProRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the PDK/BCKDK protein kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0787 Eukaryota
COG0642 LUCA
GeneTreeiENSGT00550000074574
HOGENOMiHOG000164315
HOVERGENiHBG004829
InParanoidiQ00972
KOiK00905
OMAiADEAQYC
PhylomeDBiQ00972
TreeFamiTF331303

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.140.20, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR036784 AK/P_DHK_N_sf
IPR018955 BCDHK/PDK_N
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF10436 BCDHK_Adom3, 1 hit
PF02518 HATPase_c, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF55874 SSF55874, 1 hit
SSF69012 SSF69012, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILTSVLGSG PRSGSSLWPL LGSSLSLRVR STSATDTHHV ELARERSKTV
60 70 80 90 100
TSFYNQSAID VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV
110 120 130 140 150
RIAHRIKGFR SLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ
160 170 180 190 200
YCQLVRQLLD DHKDVVTLLA EGLRESRKHI EDEKLVRYFL DKTLTSRLGI
210 220 230 240 250
RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR LCEHKYGNAP
260 270 280 290 300
RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI
310 320 330 340 350
TIANNDVDLI IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG
360 370 380 390 400
HLDMHSGGQS GPMHGFGFGL PTSRAYAEYL GGSLQLQSLQ GIGTDVYLRL
410
RHIDGREESF RI
Length:412
Mass (Da):46,474
Last modified:July 15, 1998 - v2
Checksum:iD630D3A44728927E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 124RSLPF…PTILH → VVFLSSLVATLPYCT (PubMed:1377677).CuratedAdd BLAST15
Sequence conflicti110 – 124RSLPF…PTILH → VVFLSSLVATLPYCT (PubMed:1496922).CuratedAdd BLAST15
Sequence conflicti222C → S (PubMed:1377677).Curated1
Sequence conflicti222C → S (PubMed:1496922).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93271 mRNA Translation: AAA40818.1
U27456 mRNA Translation: AAB60498.1
PIRiA42924
RefSeqiNP_062117.2, NM_019244.2
UniGeneiRn.31976

Genome annotation databases

EnsembliENSRNOT00000078578; ENSRNOP00000069014; ENSRNOG00000019485
GeneIDi29603
KEGGirno:29603
UCSCiRGD:2198 rat

Entry informationi

Entry nameiBCKD_RAT
AccessioniPrimary (citable) accession number: Q00972
Secondary accession number(s): Q64552
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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