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Q00972

- BCKD_RAT

UniProt

Q00972 - BCKD_RAT

Protein

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Gene

Bckdk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

    Catalytic activityi

    ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.

    GO - Molecular functioni

    1. [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity Source: RGD
    2. ATP binding Source: HGNC
    3. protein serine/threonine kinase activity Source: HGNC

    GO - Biological processi

    1. branched-chain amino acid catabolic process Source: HGNC
    2. phosphorylation Source: HGNC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial (EC:2.7.11.4)
    Alternative name(s):
    Branched-chain alpha-ketoacid dehydrogenase kinase
    Short name:
    BCKD-kinase
    Short name:
    BCKDHKIN
    Gene namesi
    Name:Bckdk
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2198. Bckdk.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial alpha-ketoglutarate dehydrogenase complex Source: HGNC
    2. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030Mitochondrion1 PublicationAdd
    BLAST
    Chaini31 – 412382[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrialPRO_0000023454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921N6-acetyllysineBy similarity
    Modified residuei233 – 2331N6-acetyllysineBy similarity

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ00972.
    PRIDEiQ00972.

    PTM databases

    PhosphoSiteiQ00972.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00972.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi248235. 1 interaction.
    IntActiQ00972. 1 interaction.
    STRINGi10116.ENSRNOP00000026466.

    Structurei

    Secondary structure

    1
    412
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi69 – 735
    Beta strandi74 – 763
    Beta strandi82 – 843
    Helixi85 – 11127
    Helixi114 – 1174
    Helixi120 – 13819
    Helixi145 – 16117
    Turni162 – 1643
    Helixi165 – 17612
    Helixi177 – 1793
    Helixi184 – 20926
    Beta strandi221 – 2255
    Helixi227 – 24620
    Beta strandi252 – 2576
    Beta strandi262 – 2643
    Helixi267 – 28620
    Beta strandi298 – 3047
    Beta strandi306 – 3149
    Helixi322 – 3254
    Turni326 – 3294
    Helixi369 – 37911
    Beta strandi383 – 3897
    Turni390 – 3923
    Beta strandi393 – 4019
    Beta strandi403 – 4053

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GJVX-ray2.70A31-412[»]
    1GKXX-ray2.30A31-412[»]
    1GKZX-ray2.20A31-412[»]
    3TZ0X-ray2.50A1-412[»]
    3TZ2X-ray2.85A1-412[»]
    3TZ4X-ray2.25A1-412[»]
    3TZ5X-ray2.40A1-412[»]
    3VADX-ray2.60A1-412[»]
    4DZYX-ray2.10A1-412[»]
    4E00X-ray2.15A1-412[»]
    4E01X-ray1.97A1-412[»]
    4E02X-ray2.15A1-412[»]
    4H7QX-ray2.10A1-412[»]
    4H81X-ray2.05A1-412[»]
    4H85X-ray2.10A1-412[»]
    ProteinModelPortaliQ00972.
    SMRiQ00972. Positions 68-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00972.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini159 – 404246Histidine kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PDK/BCKDK protein kinase family.Curated
    Contains 1 histidine kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0642.
    GeneTreeiENSGT00550000074574.
    HOGENOMiHOG000164315.
    HOVERGENiHBG004829.
    InParanoidiQ00972.
    KOiK00905.
    OMAiTIANNDV.
    OrthoDBiEOG7TQV0S.
    PhylomeDBiQ00972.
    TreeFamiTF331303.

    Family and domain databases

    Gene3Di1.20.140.20. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR018955. BCDHK/PDK_N.
    IPR003594. HATPase_ATP-bd.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view]
    PfamiPF10436. BCDHK_Adom3. 1 hit.
    PF02518. HATPase_c. 1 hit.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF55874. SSF55874. 1 hit.
    SSF69012. SSF69012. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILTSVLGSG PRSGSSLWPL LGSSLSLRVR STSATDTHHV ELARERSKTV    50
    TSFYNQSAID VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV 100
    RIAHRIKGFR SLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ 150
    YCQLVRQLLD DHKDVVTLLA EGLRESRKHI EDEKLVRYFL DKTLTSRLGI 200
    RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR LCEHKYGNAP 250
    RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI 300
    TIANNDVDLI IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG 350
    HLDMHSGGQS GPMHGFGFGL PTSRAYAEYL GGSLQLQSLQ GIGTDVYLRL 400
    RHIDGREESF RI 412
    Length:412
    Mass (Da):46,474
    Last modified:July 15, 1998 - v2
    Checksum:iD630D3A44728927E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 12415RSLPF…PTILH → VVFLSSLVATLPYCT(PubMed:1377677)CuratedAdd
    BLAST
    Sequence conflicti110 – 12415RSLPF…PTILH → VVFLSSLVATLPYCT(PubMed:1496922)CuratedAdd
    BLAST
    Sequence conflicti222 – 2221C → S(PubMed:1377677)Curated
    Sequence conflicti222 – 2221C → S(PubMed:1496922)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93271 mRNA. Translation: AAA40818.1.
    U27456 mRNA. Translation: AAB60498.1.
    PIRiA42924.
    RefSeqiNP_062117.2. NM_019244.2.
    UniGeneiRn.31976.

    Genome annotation databases

    EnsembliENSRNOT00000026466; ENSRNOP00000026466; ENSRNOG00000019485.
    GeneIDi29603.
    KEGGirno:29603.
    UCSCiRGD:2198. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93271 mRNA. Translation: AAA40818.1 .
    U27456 mRNA. Translation: AAB60498.1 .
    PIRi A42924.
    RefSeqi NP_062117.2. NM_019244.2.
    UniGenei Rn.31976.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GJV X-ray 2.70 A 31-412 [» ]
    1GKX X-ray 2.30 A 31-412 [» ]
    1GKZ X-ray 2.20 A 31-412 [» ]
    3TZ0 X-ray 2.50 A 1-412 [» ]
    3TZ2 X-ray 2.85 A 1-412 [» ]
    3TZ4 X-ray 2.25 A 1-412 [» ]
    3TZ5 X-ray 2.40 A 1-412 [» ]
    3VAD X-ray 2.60 A 1-412 [» ]
    4DZY X-ray 2.10 A 1-412 [» ]
    4E00 X-ray 2.15 A 1-412 [» ]
    4E01 X-ray 1.97 A 1-412 [» ]
    4E02 X-ray 2.15 A 1-412 [» ]
    4H7Q X-ray 2.10 A 1-412 [» ]
    4H81 X-ray 2.05 A 1-412 [» ]
    4H85 X-ray 2.10 A 1-412 [» ]
    ProteinModelPortali Q00972.
    SMRi Q00972. Positions 68-408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248235. 1 interaction.
    IntActi Q00972. 1 interaction.
    STRINGi 10116.ENSRNOP00000026466.

    PTM databases

    PhosphoSitei Q00972.

    Proteomic databases

    PaxDbi Q00972.
    PRIDEi Q00972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026466 ; ENSRNOP00000026466 ; ENSRNOG00000019485 .
    GeneIDi 29603.
    KEGGi rno:29603.
    UCSCi RGD:2198. rat.

    Organism-specific databases

    CTDi 10295.
    RGDi 2198. Bckdk.

    Phylogenomic databases

    eggNOGi COG0642.
    GeneTreei ENSGT00550000074574.
    HOGENOMi HOG000164315.
    HOVERGENi HBG004829.
    InParanoidi Q00972.
    KOi K00905.
    OMAi TIANNDV.
    OrthoDBi EOG7TQV0S.
    PhylomeDBi Q00972.
    TreeFami TF331303.

    Miscellaneous databases

    EvolutionaryTracei Q00972.
    NextBioi 609772.
    PROi Q00972.

    Gene expression databases

    Genevestigatori Q00972.

    Family and domain databases

    Gene3Di 1.20.140.20. 1 hit.
    3.30.565.10. 1 hit.
    InterProi IPR018955. BCDHK/PDK_N.
    IPR003594. HATPase_ATP-bd.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view ]
    Pfami PF10436. BCDHK_Adom3. 1 hit.
    PF02518. HATPase_c. 1 hit.
    [Graphical view ]
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55874. SSF55874. 1 hit.
    SSF69012. SSF69012. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases."
      Popov K.M., Zhao Y., Shimomura Y., Kuntz M.J., Harris R.A.
      J. Biol. Chem. 267:13127-13130(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-67; 93-100; 254-260 AND 328-343.
      Tissue: Heart.
    2. "Purification, characterization, regulation and molecular cloning of mitochondrial protein kinases."
      Harris R.A., Popov K.M., Shimomura Y., Zhao Y., Jaskiewicz J., Nanaumi N., Suzuki M.
      Adv. Enzyme Regul. 32:267-284(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    3. "Expression and characterization of branched-chain alpha-ketoacid dehydrogenase kinase from the rat. Is it a histidine-protein kinase?"
      Davie J.R., Wynn R.M., Meng M., Huang Y.-S., Aalund G., Chuang D.T., Lau K.S.
      J. Biol. Chem. 270:19861-19867(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-412, SEQUENCE REVISION TO 110-124 AND 222.
      Tissue: Kidney.

    Entry informationi

    Entry nameiBCKD_RAT
    AccessioniPrimary (citable) accession number: Q00972
    Secondary accession number(s): Q64552
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3