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Protein

Neutral protease

Gene

nprV

Organism
Vibrio proteolyticus (Aeromonas proteolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favored residue, which distinguished this enzyme from thermolysin.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi343Zinc; catalyticPROSITE-ProRule annotation1
Active sitei344PROSITE-ProRule annotation1
Metal bindingi347Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi367Zinc; catalyticPROSITE-ProRule annotation1
Active sitei426Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.25. 167.

Protein family/group databases

MEROPSiM04.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral protease (EC:3.4.24.25)
Alternative name(s):
Aeromonolysin
Vibriolysin
Gene namesi
Name:nprV
OrganismiVibrio proteolyticus (Aeromonas proteolytica)
Taxonomic identifieri671 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002863025 – 196Sequence analysisAdd BLAST172
ChainiPRO_0000028631197 – 609Neutral proteaseAdd BLAST413

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ00971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK08604.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR007280. Peptidase_C_arc/bac.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTQRHINW LLAVSAATAL PVTAAEMINV NDGSLLNQAL KAQSQSVAPV
60 70 80 90 100
ETGFKQMKRV VLPNGKVKVR YQQTHHGLPV FNTSVVATES KSGSSEVFGV
110 120 130 140 150
MAQGIADDVS TLTPSVEMKQ AISIAKSRFQ QQEKMVAEPA TENEKAELMV
160 170 180 190 200
RLDDNNQAQL VYLVDFFVAE DHPARPFFFI DAQTGEVLQT WDGLNHAQAD
210 220 230 240 250
GTGPGGNTKT GRYEYGSDFP PFVIDKVGTK CSMNNSAVRT VDLNGSTSGN
260 270 280 290 300
TTYSYTCNDS TNYNDYKAIN GAYSPLNDAH YFGKVVFDMY KDWMNTTPLT
310 320 330 340 350
FQLTMRVHYG NNYENAFWNG SSMTFGDGYS TFYPLVDINV SAHEVSHGFT
360 370 380 390 400
EQNSGLVYEN MSGGMNEAFS DIAGEAAEFY MKGSVDWVVG ADIFKSSGGL
410 420 430 440 450
RYFDQPSRDG RSIDHASDYY NGLNVHYSSG VFNRAFYLLA NKAGWDVRKG
460 470 480 490 500
FEVFTLANQL YWTANSTFDE GGCGVVKAAS DMGYSVADVE DAFNTVGVNA
510 520 530 540 550
SCGATPPPSG DVLEIGKPLA NLSGNRNDMT YYTFTPSSSS SVVIKITGGT
560 570 580 590 600
GDADLYVKAG SKPTTTSYDC RPYKYGNEEQ CSISAQAGTT YHVMLRGYSN

YAGVTLRAD
Length:609
Mass (Da):66,363
Last modified:April 1, 1993 - v1
Checksum:i8FAB1798D737FC42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64809 Genomic DNA. Translation: AAA27548.1.
PIRiJT0903.

Genome annotation databases

KEGGiag:AAA27548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64809 Genomic DNA. Translation: AAA27548.1.
PIRiJT0903.

3D structure databases

ProteinModelPortaliQ00971.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM04.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA27548.

Phylogenomic databases

KOiK08604.

Enzyme and pathway databases

BRENDAi3.4.24.25. 167.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR007280. Peptidase_C_arc/bac.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRV_VIBPR
AccessioniPrimary (citable) accession number: Q00971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.