Reviewed,
UniProtKB/Swiss-Prot Q00960 (NMDE2_RAT)
Last modified
November 25, 2008.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutamate [NMDA] receptor subunit epsilon-2 Alternative name(s): N-methyl D-aspartate receptor subtype 2B Short name=NMDAR2B Short name=NR2B | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1482 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. |
| Subunit structure | Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3 By similarity. Interacts with PDZ domains of INADL and DLG4. |
| Subcellular location | Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. |
| Sequence similarities | Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification] |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Dlg4 | P31016 | 3 | EBI-396905,EBI-375655 | |
| Lrfn2 | Q460M5 | 1 | EBI-396905,EBI-877185 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Potential | ||||||
| Chain | 27 – 1482 | 1456 | Glutamate [NMDA] receptor subunit epsilon-2 | PRO_0000011579 | |||||
Regions | |||||||||
| Topological domain | 27 – 557 | 531 | Extracellular Potential | ||||||
| Transmembrane | 558 – 578 | 21 | Potential | ||||||
| Topological domain | 579 – 634 | 56 | Cytoplasmic Potential | ||||||
| Transmembrane | 635 – 655 | 21 | Potential | ||||||
| Topological domain | 656 – 817 | 162 | Extracellular Potential | ||||||
| Transmembrane | 818 – 838 | 21 | Potential | ||||||
| Topological domain | 839 – 1482 | 644 | Cytoplasmic Potential | ||||||
| Motif | 1480 – 1482 | 3 | PDZ-binding | ||||||
| Compositional bias | 984 – 989 | 6 | Poly-His | ||||||
Sites | |||||||||
| Site | 615 | 1 | Functional determinant of NMDA receptors By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 917 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 930 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 932 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 949 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 962 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1039 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1049 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1061 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1065 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 1070 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1109 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1155 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1166 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1252 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1303 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1304 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1472 | 1 | Phosphotyrosine By similarity | ||||||
| Glycosylation | 74 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 341 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 348 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 444 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 491 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 542 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 688 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 1256 | 1 | E → K in AAA50554. Ref.2 | ||||||
| Sequence conflict | 1430 – 1431 | 2 | VT → SA in AAA50554. Ref.2 | ||||||
Sequences
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References
| [1] | "Heteromeric NMDA receptors: molecular and functional distinction of subtypes." Monyer H., Sprengel R., Schoepfer R., Herb A., Higuchi M., Lomeli H., Burnashev N., Sakmann B., Seeburg P.H. Science 256:1217-1221(1992) [PubMed: 1350383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | "Identification of two cysteine residues that are required for redox modulation of the NMDA subtype of glutamate receptor." Sullivan J.M., Traynelis S.F., Chen H.S., Escobar W., Heinemann S.F., Lipton S.A. Neuron 13:929-936(1994) [PubMed: 7524561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95." Kornau H.C., Schenker L.T., Kennedy M.B., Seeburg P.H. Science 269:1737-1740(1995) [PubMed: 7569905] [Abstract] Cited for: INTERACTION WITH DLG4. |
| [4] | "CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins." Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J. Mol. Cell. Neurosci. 11:161-172(1998) [PubMed: 9647694] [Abstract] Cited for: INTERACTION WITH INADL. |
| [5] | "An NMDA receptor signaling complex with protein phosphatase 2A." Chan S.F., Sucher N.J. J. Neurosci. 21:7985-7992(2001) [PubMed: 11588171] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN3A AND PPP2CB. |
| [6] | "Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors." Perez-Otano I., Schulteis C.T., Contractor A., Lipton S.A., Trimmer J.S., Sucher N.J., Heinemann S.F. J. Neurosci. 21:1228-1237(2001) [PubMed: 11160393] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A. |
| [7] | "Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits." Al-Hallaq R.A., Jarabek B.R., Fu Z., Vicini S., Wolfe B.B., Yasuda R.P. Mol. Pharmacol. 62:1119-1127(2002) [PubMed: 12391275] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A. |
| [8] | "Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons." Sasaki Y.F., Rothe T., Premkumar L.S., Das S., Cui J., Talantova M.V., Wong H.-K., Gong X., Chan S.F., Zhang D., Nakanishi N., Sucher N.J., Lipton S.A. J. Neurophysiol. 87:2052-2063(2002) [PubMed: 11929923] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A. |
Cross-references
Sequence databases | |
|---|---|
| M91562 mRNA. Translation: AAA41714.1. U11419 mRNA. Translation: AAA50554.1. | |
| PIR | B43274. |
| RefSeq | NP_036706.1. |
| UniGene | Rn.9711 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FTK based on UniProtKB P19491. |
| SMR | Q00960. Positions 404-540, 662-802. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q00960. |
PTM databases | |
| PhosphoSite | Q00960. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000008766. Rattus norvegicus. [Contig view] |
| GeneID | 24410. |
| KEGG | rno:24410. |
Organism-specific databases | |
| RGD | 2738. Grin2b. |
Phylogenomic databases | |
| HOVERGEN | Q00960. |
Gene expression databases | |
| ArrayExpress | Q00960. |
| GermOnline | ENSRNOG00000008766. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR015683. Glutamate_receptor-rel. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. [Graphical view] |
| PANTHER | PTHR18966. Glut_Rec_Related. 1 hit. |
| Pfam | PF00060. Lig_chan. 1 hit. [Graphical view] |
| PRINTS | PR00177. NMDARECEPTOR. |
| SMART | SM00079. PBPe. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 603231. |
Entry information
| Entry name | NMDE2_RAT | ||||||||
| Accession | Primary (citable) accession number: Q00960 Secondary accession number(s): Q62684 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


