Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q00960 (NMDE2_RAT)

Last modified November 25, 2008. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Glutamate [NMDA] receptor subunit epsilon-2
Alternative name(s):
    N-methyl D-aspartate receptor subtype 2B
      Short name=NMDAR2B
      Short name=NR2B
Gene names
Name: Grin2b
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Hide | Top

Protein attributes

Sequence length1482 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit structure

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3 By similarity. Interacts with PDZ domains of INADL and DLG4.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg4P310163EBI-396905,EBI-375655
Lrfn2Q460M51EBI-396905,EBI-877185

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 14821456Glutamate [NMDA] receptor subunit epsilon-2
PRO_0000011579

Regions

Topological domain27 – 557531Extracellular Potential
Transmembrane558 – 57821 Potential
Topological domain579 – 63456Cytoplasmic Potential
Transmembrane635 – 65521 Potential
Topological domain656 – 817162Extracellular Potential
Transmembrane818 – 83821 Potential
Topological domain839 – 1482644Cytoplasmic Potential
Motif1480 – 14823PDZ-binding
Compositional bias984 – 9896Poly-His

Sites

Site6151Functional determinant of NMDA receptors By similarity

Amino acid modifications

Modified residue9171Phosphoserine By similarity
Modified residue9301Phosphoserine By similarity
Modified residue9321Phosphotyrosine By similarity
Modified residue9491Phosphotyrosine By similarity
Modified residue9621Phosphotyrosine By similarity
Modified residue10391Phosphotyrosine By similarity
Modified residue10491Phosphotyrosine By similarity
Modified residue10611Phosphoserine By similarity
Modified residue10651Phosphothreonine By similarity
Modified residue10701Phosphotyrosine By similarity
Modified residue11091Phosphotyrosine By similarity
Modified residue11551Phosphotyrosine By similarity
Modified residue11661Phosphoserine By similarity
Modified residue12521Phosphotyrosine By similarity
Modified residue13031Phosphoserine By similarity
Modified residue13041Phosphotyrosine By similarity
Modified residue14721Phosphotyrosine By similarity
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation3411N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation4441N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Glycosylation5421N-linked (GlcNAc...) Potential
Glycosylation6881N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict12561E → K in AAA50554. Ref.2
Sequence conflict1430 – 14312VT → SA in AAA50554. Ref.2

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q00960-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: AEF8B9DF3C1B0D5D

FASTA1,482166,071
        10         20         30         40         50         60 
MKPSAECCSP KFWLVLAVLA VSGSKARSQK SPPSIGIAVI LVGTSDEVAI KDAHEKDDFH 

        70         80         90        100        110        120 
HLSVVPRVEL VAMNETDPKS IITRICDLMS DRKIQGVVFA DDTDQEAIAQ ILDFISAQTL 

       130        140        150        160        170        180 
TPILGIHGGS SMIMADKDES SMFFQFGPSI EQQASVMLNI MEEYDWYIFS IVTTYFPGYQ 

       190        200        210        220        230        240 
DFVNKIRSTI ENSFVGWELE EVLLLDMSLD DGDSKIQNQL KKLQSPIILL YCTKEEATYI 

       250        260        270        280        290        300 
FEVANSVGLT GYGYTWIVPS LVAGDTDTVP SEFPTGLISV SYDEWDYGLP ARVRDGIAII 

       310        320        330        340        350        360 
TTAASDMLSE HSFIPEPKSS CYNTHEKRIY QSNMLNRYLI NVTFEGRNLS FSEDGYQMHP 

       370        380        390        400        410        420 
KLVIILLNKE RKWERVGKWK DKSLQMKYYV WPRMCPETEE QEDDHLSIVT LEEAPFVIVE 

       430        440        450        460        470        480 
SVDPLSGTCM RNTVPCQKRI ISENKTDEEP GYIKKCCKGF CIDILKKISK SVKFTYDLYL 

       490        500        510        520        530        540 
VTNGKHGKKI NGTWNGMIGE VVMKRAYMAV GSLTINEERS EVVDFSVPFI ETGISVMVSR 

       550        560        570        580        590        600 
SNGTVSPSAF LEPFSADVWV MMFVMLLIVS AVAVFVFEYF SPVGYNRCLA DGREPGGPSF 

       610        620        630        640        650        660 
TIGKAIWLLW GLVFNNSVPV QNPKGTTSKI MVSVWAFFAV IFLASYTANL AAFMIQEEYV 

       670        680        690        700        710        720 
DQVSGLSDKK FQRPNDFSPP FRFGTVPNGS TERNIRNNYA EMHAYMGKFN QRGVDDALLS 

       730        740        750        760        770        780 
LKTGKLDAFI YDAAVLNYMA GRDEGCKLVT IGSGKVFAST GYGIAIQKDS GWKRQVDLAI 

       790        800        810        820        830        840 
LQLFGDGEME ELEALWLTGI CHNEKNEVMS SQLDIDNMAG VFYMLGAAMA LSLITFICEH 

       850        860        870        880        890        900 
LFYWQFRHCF MGVCSGKPGM VFSISRGIYS CIHGVAIEER QSVMNSPTAT MNNTHSNILR 

       910        920        930        940        950        960 
LLRTAKNMAN LSGVNGSPQS ALDFIRRESS VYDISEHRRS FTHSDCKSYN NPPCEENLFS 

       970        980        990       1000       1010       1020 
DYISEVERTF GNLQLKDSNV YQDHYHHHHR PHSIGSTSSI DGLYDCDNPP FTTQPRSISK 

      1030       1040       1050       1060       1070       1080 
KPLDIGLPSS KHSQLSDLYG KFSFKSDRYS GHDDLIRSDV SDISTHTVTY GNIEGNAAKR 

      1090       1100       1110       1120       1130       1140 
RKQQYKDSLK KRPASAKSRR EFDEIELAYR RRPPRSPDHK RYFRDKEGLR DFYLDQFRTK 

      1150       1160       1170       1180       1190       1200 
ENSPHWEHVD LTDIYKERSD DFKRDSVSGG GPCTNRSHLK HGTGEKHGVV GGVPAPWEKN 

      1210       1220       1230       1240       1250       1260 
LTNVDWEDRS GGNFCRSCPS KLHNYSSTVA GQNSGRQACI RCEACKKAGN LYDISEDNSL 

      1270       1280       1290       1300       1310       1320 
QELDQPAAPV AVTSNASSTK YPQSPTNSKA QKKNRNKLRR QHSYDTFVDL QKEEAALAPR 

      1330       1340       1350       1360       1370       1380 
SVSLKDKGRF MDGSPYAHMF EMPAGESSFA NKSSVPTAGH HHNNPGSGYM LSKSLYPDRV 

      1390       1400       1410       1420       1430       1440 
TQNPFIPTFG DDQCLLHGSK SYFFRQPTVA GASKTRPDFR ALVTNKPVVV TLHGAVPGRF 

      1450       1460       1470       1480 
QKDICIGNQS NPCVPNNKNP RAFNGSSNGH VYEKLSSIES DV

« Hide

Hide | Top

References

[1]"Heteromeric NMDA receptors: molecular and functional distinction of subtypes."
Monyer H., Sprengel R., Schoepfer R., Herb A., Higuchi M., Lomeli H., Burnashev N., Sakmann B., Seeburg P.H.
Science 256:1217-1221(1992) [PubMed: 1350383] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Identification of two cysteine residues that are required for redox modulation of the NMDA subtype of glutamate receptor."
Sullivan J.M., Traynelis S.F., Chen H.S., Escobar W., Heinemann S.F., Lipton S.A.
Neuron 13:929-936(1994) [PubMed: 7524561] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95."
Kornau H.C., Schenker L.T., Kennedy M.B., Seeburg P.H.
Science 269:1737-1740(1995) [PubMed: 7569905] [Abstract]
Cited for: INTERACTION WITH DLG4.
[4]"CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins."
Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J.
Mol. Cell. Neurosci. 11:161-172(1998) [PubMed: 9647694] [Abstract]
Cited for: INTERACTION WITH INADL.
[5]"An NMDA receptor signaling complex with protein phosphatase 2A."
Chan S.F., Sucher N.J.
J. Neurosci. 21:7985-7992(2001) [PubMed: 11588171] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN3A AND PPP2CB.
[6]"Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors."
Perez-Otano I., Schulteis C.T., Contractor A., Lipton S.A., Trimmer J.S., Sucher N.J., Heinemann S.F.
J. Neurosci. 21:1228-1237(2001) [PubMed: 11160393] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.
[7]"Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits."
Al-Hallaq R.A., Jarabek B.R., Fu Z., Vicini S., Wolfe B.B., Yasuda R.P.
Mol. Pharmacol. 62:1119-1127(2002) [PubMed: 12391275] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.
[8]"Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons."
Sasaki Y.F., Rothe T., Premkumar L.S., Das S., Cui J., Talantova M.V., Wong H.-K., Gong X., Chan S.F., Zhang D., Nakanishi N., Sucher N.J., Lipton S.A.
J. Neurophysiol. 87:2052-2063(2002) [PubMed: 11929923] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3A.

Hide | Top

Cross-references

Sequence databases

M91562 mRNA. Translation: AAA41714.1.
U11419 mRNA. Translation: AAA50554.1.
PIRB43274.
RefSeqNP_036706.1.
UniGeneRn.9711

3D structure databases

HSSPHSSP built from PDB template 1FTK based on UniProtKB P19491.
SMRQ00960. Positions 404-540, 662-802.
ModBaseSearch...

Protein-protein interaction databases

IntActQ00960.

PTM databases

PhosphoSiteQ00960.

Genome annotation databases

EnsemblENSRNOG00000008766. Rattus norvegicus. [Contig view]
GeneID24410.
KEGGrno:24410.

Organism-specific databases

RGD2738. Grin2b.

Phylogenomic databases

HOVERGENQ00960.

Gene expression databases

ArrayExpressQ00960.
GermOnlineENSRNOG00000008766. Rattus norvegicus.

Family and domain databases

InterProIPR015683. Glutamate_receptor-rel.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
[Graphical view]
PANTHERPTHR18966. Glut_Rec_Related. 1 hit.
PfamPF00060. Lig_chan. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00079. PBPe. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio603231.

Hide | Top

Entry information

Entry nameNMDE2_RAT
AccessionPrimary (citable) accession number: Q00960
Secondary accession number(s): Q62684
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 25, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents