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Protein

Protein LEAFY

Gene

LFY

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1. Is required subsequently for the transition of an inflorescence meristem into a floral meristem, by an immediate upstream regulation of the ABC classes of floral homeotic genes. Activates directly APETALA1, CAULIFLOWER and AGAMOUS, and indirectly APETALA3 and PISTILLATA with the cooperation of UFO.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi233 – 2375
DNA bindingi302 – 3098
DNA bindingi373 – 3764

GO - Molecular functioni

  • chromatin DNA binding Source: TAIR
  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: TAIR
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • floral meristem determinacy Source: TAIR
  • flower development Source: TAIR
  • gibberellic acid mediated signaling pathway Source: TAIR
  • maintenance of inflorescence meristem identity Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein LEAFY
Gene namesi
Name:LFY
Ordered Locus Names:At5g61850
ORF Names:MAC9.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G61850.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233R → A: Impaired DNA-binding. 1 Publication1
Mutagenesisi234E → K in lfy-4; decreased in vitro DNA-binding affinities and leafy phenotype. 1 Publication1
Mutagenesisi236P → L in lfy-5; decreased in vitro DNA-binding affinities and leafy phenotype. 1 Publication1
Mutagenesisi240T → M in lfy-3; leafy phenotype. 1 Publication1
Mutagenesisi287N → A: Impaired DNA-binding. 1 Publication1
Mutagenesisi302N → D in lfy-20; reduced DNA-binding affinity with a weak leafy phenotype. 1 Publication1
Mutagenesisi303K → A: Impaired DNA-binding. 1 Publication1
Mutagenesisi304P → L in lfy-28; impaired DNA-binding and strong leafy phenotype. 1 Publication1
Mutagenesisi327R → K in lfy-9; leafy phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001291471 – 420Protein LEAFYAdd BLAST420

Proteomic databases

PaxDbiQ00958.

PTM databases

iPTMnetiQ00958.

Expressioni

Tissue specificityi

Expressed uniformly throughout the young floral primordia.

Developmental stagei

Expressed at an early stage of floral initiation.

Inductioni

Positively regulated by CAULIFLOWER and APETALA1. Down-regulated by TFL1.

Gene expression databases

ExpressionAtlasiQ00958. baseline and differential.
GenevisibleiQ00958. AT.

Interactioni

Subunit structurei

Forms homodimer when associated to DNA. Interacts with SYD and BRM.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei280Interaction with DNA1
Sitei287Interaction with DNA1
Sitei291Interaction with DNA1
Sitei338Interaction with DNA1

Binary interactionsi

WithEntry#Exp.IntActNotes
UFOQ390906EBI-1644366,EBI-590758

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: TAIR

Protein-protein interaction databases

BioGridi21551. 5 interactors.
DIPiDIP-40341N.
IntActiQ00958. 1 interactor.
STRINGi3702.AT5G61850.1.

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi253 – 276Combined sources24
Helixi287 – 295Combined sources9
Helixi303 – 308Combined sources6
Helixi310 – 318Combined sources9
Helixi320 – 332Combined sources13
Helixi337 – 343Combined sources7
Helixi345 – 353Combined sources9
Turni354 – 357Combined sources4
Helixi359 – 365Combined sources7
Helixi369 – 371Combined sources3
Helixi376 – 392Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VY1X-ray2.10A227-420[»]
2VY2X-ray2.30A227-420[»]
ProteinModelPortaliQ00958.
SMRiQ00958.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00958.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili108 – 133Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 29Poly-Pro7
Compositional biasi114 – 130Arg/Glu-richAdd BLAST17
Compositional biasi204 – 216Asp/Glu-rich (acidic)Add BLAST13

Sequence similaritiesi

Belongs to the FLO/LFY family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IE4M. Eukaryota.
ENOG4110P9Q. LUCA.
HOGENOMiHOG000083786.
InParanoidiQ00958.
OMAiDMQGDEH.
OrthoDBiEOG09360J2T.
PhylomeDBiQ00958.

Family and domain databases

InterProiIPR002910. FLO_LFY.
[Graphical view]
PANTHERiPTHR36079:SF1. PTHR36079:SF1. 1 hit.
PfamiPF01698. FLO_LFY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPEGFTSGL FRWNPTRALV QAPPPVPPPL QQQPVTPQTA AFGMRLGGLE
60 70 80 90 100
GLFGPYGIRF YTAAKIAELG FTASTLVGMK DEELEEMMNS LSHIFRWELL
110 120 130 140 150
VGERYGIKAA VRAERRRLQE EEEEESSRRR HLLLSAAGDS GTHHALDALS
160 170 180 190 200
QEGLSEEPVQ QQDQTDAAGN NGGGGSGYWD AGQGKMKKQQ QQRRRKKPML
210 220 230 240 250
TSVETDEDVN EGEDDDGMDN GNGGSGLGTE RQREHPFIVT EPGEVARGKK
260 270 280 290 300
NGLDYLFHLY EQCREFLLQV QTIAKDRGEK CPTKVTNQVF RYAKKSGASY
310 320 330 340 350
INKPKMRHYV HCYALHCLDE EASNALRRAF KERGENVGSW RQACYKPLVN
360 370 380 390 400
IACRHGWDID AVFNAHPRLS IWYVPTKLRQ LCHLERNNAV AAAAALVGGI
410 420
SCTGSSTSGR GGCGGDDLRF
Length:420
Mass (Da):46,582
Last modified:September 22, 2009 - v2
Checksum:i952268D52E035CFF
GO

Sequence cautioni

The sequence AAA32826 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27927 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27928 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27929 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27930 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27931 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27932 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27933 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27934 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27935 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27936 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27937 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27938 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27939 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27940 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAM27941 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABK28773 differs from that shown. Reason: Erroneous termination at position 421. Translated as stop.Curated
The sequence BAB10085 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8S → R in strain: cv. Jl-1. 1
Natural varianti78G → D in strain: cv. Ita-0. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91208 Genomic DNA. Translation: AAA32826.1. Sequence problems.
AF466787 Genomic DNA. Translation: AAM27927.1. Sequence problems.
AF466788 Genomic DNA. Translation: AAM27928.1. Sequence problems.
AF466789 Genomic DNA. Translation: AAM27929.1. Sequence problems.
AF466790 Genomic DNA. Translation: AAM27930.1. Sequence problems.
AF466791 Genomic DNA. Translation: AAM27931.1. Sequence problems.
AF466792 Genomic DNA. Translation: AAM27932.1. Sequence problems.
AF466793 Genomic DNA. Translation: AAM27933.1. Sequence problems.
AF466794 Genomic DNA. Translation: AAM27934.1. Sequence problems.
AF466795 Genomic DNA. Translation: AAM27935.1. Sequence problems.
AF466796 Genomic DNA. Translation: AAM27936.1. Sequence problems.
AF466797 Genomic DNA. Translation: AAM27937.1. Sequence problems.
AF466798 Genomic DNA. Translation: AAM27938.1. Sequence problems.
AF466799 Genomic DNA. Translation: AAM27939.1. Sequence problems.
AF466800 Genomic DNA. Translation: AAM27940.1. Sequence problems.
AF466801 Genomic DNA. Translation: AAM27941.1. Sequence problems.
AB010069 Genomic DNA. Translation: BAB10085.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97525.1.
DQ447103 mRNA. Translation: ABE66271.1.
DQ653389 mRNA. Translation: ABK28773.1. Sequence problems.
PIRiA38104.
B38104.
C38104.
RefSeqiNP_200993.1. NM_125579.1.
UniGeneiAt.55671.

Genome annotation databases

EnsemblPlantsiAT5G61850.1; AT5G61850.1; AT5G61850.
GeneIDi836307.
GrameneiAT5G61850.1; AT5G61850.1; AT5G61850.
KEGGiath:AT5G61850.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91208 Genomic DNA. Translation: AAA32826.1. Sequence problems.
AF466787 Genomic DNA. Translation: AAM27927.1. Sequence problems.
AF466788 Genomic DNA. Translation: AAM27928.1. Sequence problems.
AF466789 Genomic DNA. Translation: AAM27929.1. Sequence problems.
AF466790 Genomic DNA. Translation: AAM27930.1. Sequence problems.
AF466791 Genomic DNA. Translation: AAM27931.1. Sequence problems.
AF466792 Genomic DNA. Translation: AAM27932.1. Sequence problems.
AF466793 Genomic DNA. Translation: AAM27933.1. Sequence problems.
AF466794 Genomic DNA. Translation: AAM27934.1. Sequence problems.
AF466795 Genomic DNA. Translation: AAM27935.1. Sequence problems.
AF466796 Genomic DNA. Translation: AAM27936.1. Sequence problems.
AF466797 Genomic DNA. Translation: AAM27937.1. Sequence problems.
AF466798 Genomic DNA. Translation: AAM27938.1. Sequence problems.
AF466799 Genomic DNA. Translation: AAM27939.1. Sequence problems.
AF466800 Genomic DNA. Translation: AAM27940.1. Sequence problems.
AF466801 Genomic DNA. Translation: AAM27941.1. Sequence problems.
AB010069 Genomic DNA. Translation: BAB10085.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97525.1.
DQ447103 mRNA. Translation: ABE66271.1.
DQ653389 mRNA. Translation: ABK28773.1. Sequence problems.
PIRiA38104.
B38104.
C38104.
RefSeqiNP_200993.1. NM_125579.1.
UniGeneiAt.55671.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VY1X-ray2.10A227-420[»]
2VY2X-ray2.30A227-420[»]
ProteinModelPortaliQ00958.
SMRiQ00958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21551. 5 interactors.
DIPiDIP-40341N.
IntActiQ00958. 1 interactor.
STRINGi3702.AT5G61850.1.

PTM databases

iPTMnetiQ00958.

Proteomic databases

PaxDbiQ00958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G61850.1; AT5G61850.1; AT5G61850.
GeneIDi836307.
GrameneiAT5G61850.1; AT5G61850.1; AT5G61850.
KEGGiath:AT5G61850.

Organism-specific databases

TAIRiAT5G61850.

Phylogenomic databases

eggNOGiENOG410IE4M. Eukaryota.
ENOG4110P9Q. LUCA.
HOGENOMiHOG000083786.
InParanoidiQ00958.
OMAiDMQGDEH.
OrthoDBiEOG09360J2T.
PhylomeDBiQ00958.

Miscellaneous databases

EvolutionaryTraceiQ00958.
PROiQ00958.

Gene expression databases

ExpressionAtlasiQ00958. baseline and differential.
GenevisibleiQ00958. AT.

Family and domain databases

InterProiIPR002910. FLO_LFY.
[Graphical view]
PANTHERiPTHR36079:SF1. PTHR36079:SF1. 1 hit.
PfamiPF01698. FLO_LFY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLFY_ARATH
AccessioniPrimary (citable) accession number: Q00958
Secondary accession number(s): A0MFR2
, Q1PDG5, Q8LSH2, Q8LSH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in the LEAFY gene result in the complete transformation of the first few flowers into leaves with associated shoots.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.