Protein LEAFY - Arabidopsis thaliana (Mouse-ear cress)

Contribute

Reviewed, UniProtKB/Swiss-Prot Q00958 (LFY_ARATH)

Last modified November 25, 2008. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Protein LEAFY

Gene names

Name:	LFY
Ordered Locus Names:	At5g61850
ORF Names:	MAC9.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1. Is required subsequently for the transition of an inflorescence meristem into a floral meristem, by an immediate upstream regulation of the ABC classes of floral homeotic genes. Activates directly APETALA1, CAULIFLOWER and AGAMOUS, and indirectly APETALA3 and PISTILLATA with the cooperation of UFO.
Subcellular location	NucleusProbable.
Tissue specificity	Expressed uniformly throughout the young floral primordia.
Developmental stage	Expressed at an early stage of floral initiation.
Induction	Positively regulated by CAULIFLOWER and APETALA1. Down-regulated by TFL1.
Miscellaneous	Mutations in the LEAFY gene result in the complete transformation of the first few flowers into leaves with associated shoots.
Sequence similarities	Belongs to the FLO/LFY family.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UFO	Q39090	3	EBI-1644366,EBI-590758

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

424

Protein LEAFY

PRO_0000129147

Regions

Coiled coil

26

Potential

Compositional bias

7

Poly-Pro

Compositional bias

17

Arg/Glu-rich

Compositional bias

13

Asp/Glu-rich (acidic)

Natural variations

Natural variant

1

S → R in strain: cv. Jl-1.

Natural variant

1

G → D in strain: cv. Ita-0.

Sequences

Sequence

Length

Mass (Da)

Tools

Q00958-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 246FD1263C6638AA
Show »

424

47,100

        10         20         30         40         50         60 
MDPEGFTSGL FRWNPTRALV QAPPPVPPPL QQQPVTPQTA AFGMRLGGLE GLFGPYGIRF 

        70         80         90        100        110        120 
YTAAKIAELG FTASTLVGMK DEELEEMMNS LSHIFRWELL VGERYGIKAA VRAERRRLQE 

       130        140        150        160        170        180 
EEEEESSRRR HLLLSAAGDS GTHHALDALS QEDDWTGLSE EPVQQQDQTD AAGNNGGGGS 

       190        200        210        220        230        240 
GYWDAGQGKM KKQQQQRRRK KPMLTSVETD EDVNEGEDDD GMDNGNGGSG LGTERQREHP 

       250        260        270        280        290        300 
FIVTEPGEVA RGKKNGLDYL FHLYEQCREF LLQVQTIAKD RGEKCPTKVT NQVFRYAKKS 

       310        320        330        340        350        360 
GASYINKPKM RHYVHCYALH CLDEEASNAL RRAFKERGEN VGSWRQACYK PLVNIACRHG 

       370        380        390        400        410        420 
WDIDAVFNAH PRLSIWYVPT KLRQLCHLER NNAVAAAAAL VGGISCTGSS TSGRGGCGGD 


DLRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LEAFY controls floral meristem identity in Arabidopsis." Weigel D., Alvarez J., Smyth D.R., Yanofsky M.F., Meyerowitz E.M. Cell 69:843-859(1992) [PubMed: 1350515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Contrasting evolutionary forces in the Arabidopsis thaliana floral developmental pathway." Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D. Genetics 160:1641-1650(2002) [PubMed: 11973317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS. Strain: cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, cv. Jl-1, cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Lisse and cv. Wassilewskija.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes." Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R. Development 119:721-743(1993) Cited for: FUNCTION.
[5]	"The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function." Krizek B.A., Meyerowitz E.M. Development 122:11-22(1996) [PubMed: 8565821] [Abstract] Cited for: FUNCTION.
[6]	"A genetic framework for floral patterning." Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D. Nature 395:561-566(1998) [PubMed: 9783581] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases

M91208 Genomic DNA. Translation: AAA32826.1.
AF466787 Genomic DNA. Translation: AAM27927.1.
AF466788 Genomic DNA. Translation: AAM27928.1.
AF466789 Genomic DNA. Translation: AAM27929.1.
AF466790 Genomic DNA. Translation: AAM27930.1.
AF466791 Genomic DNA. Translation: AAM27931.1.
AF466792 Genomic DNA. Translation: AAM27932.1.
AF466793 Genomic DNA. Translation: AAM27933.1.
AF466794 Genomic DNA. Translation: AAM27934.1.
AF466795 Genomic DNA. Translation: AAM27935.1.
AF466796 Genomic DNA. Translation: AAM27936.1.
AF466797 Genomic DNA. Translation: AAM27937.1.
AF466798 Genomic DNA. Translation: AAM27938.1.
AF466799 Genomic DNA. Translation: AAM27939.1.
AF466800 Genomic DNA. Translation: AAM27940.1.
AF466801 Genomic DNA. Translation: AAM27941.1.
AB010069 Genomic DNA. Translation: BAB10085.1.

PIR

A38104.
B38104.
C38104.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VY1	X-ray	2.10	A	231-424	[»]
2VY2	X-ray	2.30	A	231-424	[»]

ModBase

Protein-protein interaction databases

IntAct

Genome annotation databases

GenomeReviews

Gene locus AT5G61850 in contig BA000015_GR.

Organism-specific databases

TAIR

Gene expression databases

ArrayExpress

Family and domain databases

InterPro

IPR002910. FLO_LFY.
[Graphical view]

Pfam

PF01698. FLO_LFY. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

LFY_ARATH

Accession

Primary (citable) accession number: Q00958
Secondary accession number(s): Q8LSH2, Q8LSH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 25, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents