Protein LEAFY - Arabidopsis thaliana (Mouse-ear cress)

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Q00958 (LFY_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein LEAFY

Gene names

Name:	LFY
Ordered Locus Names:	At5g61850
ORF Names:	MAC9.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1. Is required subsequently for the transition of an inflorescence meristem into a floral meristem, by an immediate upstream regulation of the ABC classes of floral homeotic genes. Activates directly APETALA1, CAULIFLOWER and AGAMOUS, and indirectly APETALA3 and PISTILLATA with the cooperation of UFO. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed uniformly throughout the young floral primordia.
Developmental stage	Expressed at an early stage of floral initiation.
Induction	Positively regulated by CAULIFLOWER and APETALA1. Down-regulated by TFL1.
Miscellaneous	Mutations in the LEAFY gene result in the complete transformation of the first few flowers into leaves with associated shoots.
Sequence similarities	Belongs to the FLO/LFY family.
Sequence caution	The sequence AAA32826.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27927.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27928.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27929.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27930.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27931.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27932.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27933.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27934.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27935.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27936.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27937.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27938.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27939.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27940.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM27941.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABK28773.1 differs from that shown. Reason: Erroneous termination at position 421. Translated as stop. The sequence BAB10085.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from mutant phenotype. Source: TAIR gibberellic acid mediated signaling pathway Traceable author statement. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Traceable author statement. Source: TAIR
Molecular function	chromatin DNA binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: TAIR sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UFO	Q39090	6	EBI-1644366,EBI-590758

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Protein LEAFY

PRO_0000129147

Regions

Coiled coil

108 – 133

Potential

Compositional bias

23 – 29

Poly-Pro

Compositional bias

114 – 130

Arg/Glu-rich

Compositional bias

204 – 216

Asp/Glu-rich (acidic)

Natural variations

Natural variant

S → R in strain: cv. Jl-1.

Natural variant

G → D in strain: cv. Ita-0.

Secondary structure

420

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q00958 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 952268D52E035CFF
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FASTA

420

46,582

        10         20         30         40         50         60 
MDPEGFTSGL FRWNPTRALV QAPPPVPPPL QQQPVTPQTA AFGMRLGGLE GLFGPYGIRF 

        70         80         90        100        110        120 
YTAAKIAELG FTASTLVGMK DEELEEMMNS LSHIFRWELL VGERYGIKAA VRAERRRLQE 

       130        140        150        160        170        180 
EEEEESSRRR HLLLSAAGDS GTHHALDALS QEGLSEEPVQ QQDQTDAAGN NGGGGSGYWD 

       190        200        210        220        230        240 
AGQGKMKKQQ QQRRRKKPML TSVETDEDVN EGEDDDGMDN GNGGSGLGTE RQREHPFIVT 

       250        260        270        280        290        300 
EPGEVARGKK NGLDYLFHLY EQCREFLLQV QTIAKDRGEK CPTKVTNQVF RYAKKSGASY 

       310        320        330        340        350        360 
INKPKMRHYV HCYALHCLDE EASNALRRAF KERGENVGSW RQACYKPLVN IACRHGWDID 

       370        380        390        400        410        420 
AVFNAHPRLS IWYVPTKLRQ LCHLERNNAV AAAAALVGGI SCTGSSTSGR GGCGGDDLRF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LEAFY controls floral meristem identity in Arabidopsis." Weigel D., Alvarez J., Smyth D.R., Yanofsky M.F., Meyerowitz E.M. Cell 69:843-859(1992) [PubMed: 1350515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Contrasting evolutionary forces in the Arabidopsis thaliana floral developmental pathway." Olsen K.M., Womack A., Garrett A.R., Suddith J.I., Purugganan M.D. Genetics 160:1641-1650(2002) [PubMed: 11973317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS. Strain: cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0, cv. Gr-3, cv. Ita-0, cv. Jl-1, cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, cv. Lisse and cv. Wassilewskija.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes." Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M., Smyth D.R. Development 119:721-743(1993) Cited for: FUNCTION.
[7]	"The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function." Krizek B.A., Meyerowitz E.M. Development 122:11-22(1996) [PubMed: 8565821] [Abstract] Cited for: FUNCTION.
[8]	"A genetic framework for floral patterning." Parcy F., Nilsson O., Busch M.A., Lee I., Weigel D. Nature 395:561-566(1998) [PubMed: 9783581] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M91208 Genomic DNA. Translation: AAA32826.1. Sequence problems.
AF466787 Genomic DNA. Translation: AAM27927.1. Sequence problems.
AF466788 Genomic DNA. Translation: AAM27928.1. Sequence problems.
AF466789 Genomic DNA. Translation: AAM27929.1. Sequence problems.
AF466790 Genomic DNA. Translation: AAM27930.1. Sequence problems.
AF466791 Genomic DNA. Translation: AAM27931.1. Sequence problems.
AF466792 Genomic DNA. Translation: AAM27932.1. Sequence problems.
AF466793 Genomic DNA. Translation: AAM27933.1. Sequence problems.
AF466794 Genomic DNA. Translation: AAM27934.1. Sequence problems.
AF466795 Genomic DNA. Translation: AAM27935.1. Sequence problems.
AF466796 Genomic DNA. Translation: AAM27936.1. Sequence problems.
AF466797 Genomic DNA. Translation: AAM27937.1. Sequence problems.
AF466798 Genomic DNA. Translation: AAM27938.1. Sequence problems.
AF466799 Genomic DNA. Translation: AAM27939.1. Sequence problems.
AF466800 Genomic DNA. Translation: AAM27940.1. Sequence problems.
AF466801 Genomic DNA. Translation: AAM27941.1. Sequence problems.
AB010069 Genomic DNA. Translation: BAB10085.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97525.1.
DQ447103 mRNA. Translation: ABE66271.1.
DQ653389 mRNA. Translation: ABK28773.1. Sequence problems.

IPI

IPI00522122.

PIR

A38104.
B38104.
C38104.

RefSeq

NP_200993.1. NM_125579.1.

UniGene

At.55671.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VY1	X-ray	2.10	A	227-420	[»]
2VY2	X-ray	2.30	A	227-420	[»]

ProteinModelPortal

Q00958.

SMR

Q00958. Positions 233-394.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q00958. 1 interaction.

STRING

Q00958.

Proteomic databases

PRIDE

Q00958.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G61850.1; AT5G61850.1; AT5G61850.

GeneID

836307.

GenomeReviews

Gene locus AT5G61850 in contig BA000015_GR.

KEGG

ath:AT5G61850.

Organism-specific databases

TAIR

At5g61850.

Phylogenomic databases

HOGENOM

HBG596286.

InParanoid

Q00958.

OMA

HCLDEDA.

PhylomeDB

Q00958.

ProtClustDB

CLSN2686949.

Gene expression databases

ArrayExpress

Q00958.

Genevestigator

Q00958.

Family and domain databases

InterPro

IPR002910. FLO_LFY.
[Graphical view]

Pfam

PF01698. FLO_LFY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LFY_ARATH

Accession

Primary (citable) accession number: Q00958
Secondary accession number(s): A0MFR2

Q8LSH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	September 22, 2009
Last modified:	December 14, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents