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Q00954

- SCN1B_RAT

UniProt

Q00954 - SCN1B_RAT

Protein

Sodium channel subunit beta-1

Gene

Scn1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. sodium channel inhibitor activity Source: Ensembl
    3. sodium channel regulator activity Source: RGD
    4. voltage-gated sodium channel activity involved in Purkinje myocyte action potential Source: Ensembl

    GO - Biological processi

    1. cardiac muscle cell action potential involved in contraction Source: Ensembl
    2. cardiac muscle contraction Source: Ensembl
    3. corticospinal neuron axon guidance Source: Ensembl
    4. neuronal action potential propagation Source: Ensembl
    5. positive regulation of neuron projection development Source: Ensembl
    6. positive regulation of sodium ion transport Source: Ensembl
    7. regulation of atrial cardiac muscle cell membrane depolarization Source: Ensembl
    8. regulation of heart rate by cardiac conduction Source: Ensembl
    9. regulation of sodium ion transmembrane transporter activity Source: Ensembl
    10. regulation of sodium ion transport Source: RGD
    11. regulation of ventricular cardiac muscle cell membrane repolarization Source: Ensembl
    12. response to pyrethroid Source: RGD

    Keywords - Molecular functioni

    Ion channel, Sodium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium channel subunit beta-1
    Gene namesi
    Name:Scn1b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3631. Scn1b.

    Subcellular locationi

    GO - Cellular componenti

    1. intercalated disc Source: Ensembl
    2. node of Ranvier Source: Ensembl
    3. plasma membrane Source: Reactome
    4. T-tubule Source: Ensembl
    5. voltage-gated sodium channel complex Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 218200Sodium channel subunit beta-1PRO_0000014929Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi40 ↔ 1211 Publication
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ00954.
    PRIDEiQ00954.

    PTM databases

    PhosphoSiteiQ00954.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, skeletal muscle and spinal cord.

    Developmental stagei

    In developing nodes of Ranvier, it is localized in the sciatic nerve at postnantal days 3 and 10, during the process of myelination and maturation of the nodes.

    Gene expression databases

    ArrayExpressiQ00954.
    GenevestigatoriQ00954.

    Interactioni

    Subunit structurei

    The voltage-sensitive sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more beta-1, beta-2, beta-3 and/or beta-4 subunits. Beta-1 and beta-3 are non-covalently associated with alpha, while beta-2 and beta-4 are covalently linked by disulfide bonds. Beta-1 or beta-3 subunits associate with neurofascin. Associates with SCN10A.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Syt1P217072EBI-2619363,EBI-458098

    Protein-protein interaction databases

    IntActiQ00954. 2 interactions.
    MINTiMINT-205402.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00954.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 160142ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini183 – 21836CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei161 – 18222HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 150129Ig-like C2-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45207.
    GeneTreeiENSGT00390000018560.
    HOGENOMiHOG000276881.
    HOVERGENiHBG056582.
    InParanoidiQ00954.
    KOiK04845.
    OMAiISCKKRS.
    OrthoDBiEOG7M3J19.
    PhylomeDBiQ00954.
    TreeFamiTF332097.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR013783. Ig-like_fold.
    IPR013151. Immunoglobulin.
    IPR027098. Na_channel_b1/b3.
    [Graphical view]
    PANTHERiPTHR10546. PTHR10546. 1 hit.
    PfamiPF00047. ig. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGTLLALVVG AVLVSSAWGG CVEVDSETEA VYGMTFKILC ISCKRRSETT    50
    AETFTEWTFR QKGTEEFVKI LRYENEVLQL EEDERFEGRV VWNGSRGTKD 100
    LQDLSIFITN VTYNHSGDYE CHVYRLLFFD NYEHNTSVVK KIHLEVVDKA 150
    NRDMASIVSE IMMYVLIVVL TIWLVAEMVY CYKKIAAATE AAAQENASEY 200
    LAITSESKEN CTGVQVAE 218
    Length:218
    Mass (Da):24,692
    Last modified:July 1, 1993 - v1
    Checksum:i0BA84FC44FF2306B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91808 mRNA. Translation: AAA88513.1.
    BC094523 mRNA. Translation: AAH94523.1.
    PIRiA42737.
    RefSeqiNP_001257974.1. NM_001271045.1.
    NP_058984.1. NM_017288.2.
    UniGeneiRn.4958.

    Genome annotation databases

    EnsembliENSRNOT00000028653; ENSRNOP00000028653; ENSRNOG00000021102.
    GeneIDi29686.
    KEGGirno:29686.
    UCSCiRGD:3631. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91808 mRNA. Translation: AAA88513.1 .
    BC094523 mRNA. Translation: AAH94523.1 .
    PIRi A42737.
    RefSeqi NP_001257974.1. NM_001271045.1.
    NP_058984.1. NM_017288.2.
    UniGenei Rn.4958.

    3D structure databases

    ProteinModelPortali Q00954.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00954. 2 interactions.
    MINTi MINT-205402.

    PTM databases

    PhosphoSitei Q00954.

    Proteomic databases

    PaxDbi Q00954.
    PRIDEi Q00954.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028653 ; ENSRNOP00000028653 ; ENSRNOG00000021102 .
    GeneIDi 29686.
    KEGGi rno:29686.
    UCSCi RGD:3631. rat.

    Organism-specific databases

    CTDi 6324.
    RGDi 3631. Scn1b.

    Phylogenomic databases

    eggNOGi NOG45207.
    GeneTreei ENSGT00390000018560.
    HOGENOMi HOG000276881.
    HOVERGENi HBG056582.
    InParanoidi Q00954.
    KOi K04845.
    OMAi ISCKKRS.
    OrthoDBi EOG7M3J19.
    PhylomeDBi Q00954.
    TreeFami TF332097.

    Miscellaneous databases

    NextBioi 610057.
    PROi Q00954.

    Gene expression databases

    ArrayExpressi Q00954.
    Genevestigatori Q00954.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR013783. Ig-like_fold.
    IPR013151. Immunoglobulin.
    IPR027098. Na_channel_b1/b3.
    [Graphical view ]
    PANTHERi PTHR10546. PTHR10546. 1 hit.
    Pfami PF00047. ig. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of the beta 1 subunit of the rat brain sodium channel."
      Isom L.L., De Jongh K.S., Patton D.E., Reber B.F.X., Offord J., Charbonneau H., Walsh K., Goldin A.L., Catterall W.A.
      Science 256:839-842(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Sodium channel beta1 and beta3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain."
      Ratcliffe C.F., Westenbroek R.E., Curtis R., Catterall W.A.
      J. Cell Biol. 154:427-434(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH NEUROFASCIN.
    4. "Role of auxiliary beta1-, beta2-, and beta3-subunits and their interaction with Na(v)1.8 voltage-gated sodium channel."
      Vijayaragavan K., Powell A.J., Kinghorn I.J., Chahine M.
      Biochem. Biophys. Res. Commun. 319:531-540(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH SCN10A.
    5. "Identification of an intra-molecular disulfide bond in the sodium channel beta1-subunit."
      Barbieri R., Baroni D., Moran O.
      Biochem. Biophys. Res. Commun. 420:364-367(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BOND.

    Entry informationi

    Entry nameiSCN1B_RAT
    AccessioniPrimary (citable) accession number: Q00954
    Secondary accession number(s): Q505J0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3