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Protein

Hydantoin racemase

Gene

hyuE

Organism
Pseudomonas sp. (strain NS671)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the reaction cascade known as the hydantoinase process, which allows the asymmetric conversion of D,L-5-monosubstituted hydantoins into D- or L-amino acids. Catalyzes the racemization via enolization of D- and L-5-monosubstituted hydantoins. Also able to racemize 5-substituted hydantoins having either aromatic or aliphatic substituents such as D- and L-5-(2-methylthioethyl)hydantoin.2 Publications

Catalytic activityi

D-5-monosubstituted hydantoin = L-5-monosubstituted hydantoin.1 Publication

Enzyme regulationi

Strongly inhibited by Cu2+ and Zn2+. Slightly stimulated by of Mn2+ or Co2+, but also by metal-chelating agents such as EDTA or EGTA.1 Publication

Kineticsi

    1. Vmax=79 µmol/min/mg enzyme with L-5-(2-methylthioethyl)hydantoin as substrate1 Publication
    2. Vmax=35.2 µmol/min/mg enzyme with D-5-(2-methylthioethyl)hydantoin as substrate1 Publication

    pH dependencei

    Optimum pH is 9.5.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei76Seems to be responsible for recognition and proton retrieval of D-isomersBy similarity1
    Sitei181Seems to be responsible for L-isomers recognition and racemizationBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processAmino-acid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14268.
    BRENDAi5.1.99.5. 5085.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydantoin racemase1 Publication (EC:5.1.99.51 Publication)
    Gene namesi
    Name:hyuE1 Publication
    Synonyms:hyuACurated
    Encoded oniPlasmid pHN6710 Publication
    OrganismiPseudomonas sp. (strain NS671)
    Taxonomic identifieri29441 [NCBI]
    Taxonomic lineageiBacteriaProteobacteria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000841201 – 249Hydantoin racemaseAdd BLAST249

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ00924.
    SMRiQ00924.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HyuE racemase family.Curated

    Family and domain databases

    InterProiView protein in InterPro
    IPR015942. Asp/Glu/hydantoin_racemase.
    PfamiView protein in Pfam
    PF01177. Asp_Glu_race. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q00924-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIKVINPNT TLAMTKGIEH AAKSAARSDT QIVAVSPKMG PASIESYYDE
    60 70 80 90 100
    YLSIPGVIEE IKKGEEEGVD AFVIACWGDP GLHAAREVTD KPVVGIAESS
    110 120 130 140 150
    VYLASMLAAR FSVVTVLPRI KTMLEDLVDS YGMQKRVLNI RTTPMGVLDF
    160 170 180 190 200
    ERDPEAGIEM LRQEGKRAVE EDNAEAILLG CAGMAEFADS LEKELGVPVI
    210 220 230 240
    DGVVAGVKFA ETIVDLGKKT SKLKTYKYPE KKEYVGALEN FGRNQTTTK
    Length:249
    Mass (Da):27,090
    Last modified:April 1, 1993 - v1
    Checksum:iFC9A3E9E81485317
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M84731 Genomic DNA. Translation: AAA25843.1.
    PIRiB41895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M84731 Genomic DNA. Translation: AAA25843.1.
    PIRiB41895.

    3D structure databases

    ProteinModelPortaliQ00924.
    SMRiQ00924.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14268.
    BRENDAi5.1.99.5. 5085.

    Family and domain databases

    InterProiView protein in InterPro
    IPR015942. Asp/Glu/hydantoin_racemase.
    PfamiView protein in Pfam
    PF01177. Asp_Glu_race. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHYUA_PSESN
    AccessioniPrimary (citable) accession number: Q00924
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: May 10, 2017
    This is version 40 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.