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Q00916 (RU17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U1 small nuclear ribonucleoprotein 70 kDa homolog

Short name=U1 70K
Short name=U1 snRNP 70 kDa homolog
Short name=U1-70K
Alternative name(s):
U1 small nuclear ribonucleoprotein SNP1
Short name=U1 snRNP protein SNP1
Gene names
Name:SNP1
Ordered Locus Names:YIL061C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nuclear mRNA splicing.

Subunit structure

Component of the spliceosome, where it is associated with snRNP U1. Binds stem loop I of U1 snRNA. Interacts with USA1. Interacts with mRNA. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location

Nucleus By similarity.

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EXO84P382612EBI-724,EBI-21567
PRP8P333342EBI-724,EBI-465

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300U1 small nuclear ribonucleoprotein 70 kDa homolog
PRO_0000081886

Regions

Domain107 – 19892RRM

Experimental info

Mutagenesis18 – 9881Missing: Severely temperature-sensitive. Defective in pre-mRNA splicing. Ref.6
Mutagenesis18 – 9376Missing: Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP. Ref.7
Mutagenesis92 – 248157Missing: Associates with U1 snRNP. Ref.6 Ref.7
Mutagenesis1481K → L: No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152. Ref.6 Ref.7
Mutagenesis1501Y → T: No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152. Ref.6 Ref.7
Mutagenesis1521F → L: No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150. Ref.6 Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q00916 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 7D9E47BE66FE1EB8

FASTA30034,447
        10         20         30         40         50         60 
MNYNLSKYPD DVSRLFKPRP PLSYKRPTDY PYAKRQTNPN ITGVANLLST SLKHYMEEFP 

        70         80         90        100        110        120 
EGSPNNHLQR YEDIKLSKIK NAQLLDRRLQ NWNPNVDPHI KDTDPYRTIF IGRLPYDLDE 

       130        140        150        160        170        180 
IELQKYFVKF GEIEKIRIVK DKITQKSKGY AFIVFKDPIS SKMAFKEIGV HRGIQIKDRI 

       190        200        210        220        230        240 
CIVDIERGRT VKYFKPRRLG GGLGGRGYSN RDSRLPGRFA SASTSNPAER NYAPRLPRRE 

       250        260        270        280        290        300 
TSSSAYSADR YGSSTLDARY RGNRPLLSAA TPTAAVTSVY KSRNSRTRES QPAPKEAPDY 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast."
Smith V., Barrell B.G.
EMBO J. 10:2627-2634(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene."
Kao H.-Y., Siliciano P.G.
Nucleic Acids Res. 20:4009-4013(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: U1 RNA-BINDING.
[6]"The amino-terminal domain of yeast U1-70K is necessary and sufficient for function."
Hilleren P.J., Kao H.-Y., Siliciano P.G.
Mol. Cell. Biol. 15:6341-6350(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 18-PRO--PRO-98; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
[7]"The RRM domain is dispensable for yeast U1-70K function."
Hilleren P.J., Kao H.-Y., Siliciano P.G.
Nucleic Acids Symp. Ser. 33:244-247(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 18-PRO--ASN-93; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
[8]"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry."
Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M.
Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[9]"A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[10]"Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex."
Zhang D., Rosbash M.
Genes Dev. 13:581-592(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MRNA.
[11]"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EXO84; PRP8 AND USA1.
[12]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38060 Genomic DNA. Translation: CAA86162.1.
X59986 Genomic DNA. Translation: CAA42602.1.
AY558085 Genomic DNA. Translation: AAS56411.1.
BK006942 Genomic DNA. Translation: DAA08489.1.
PIRS16815.
RefSeqNP_012203.1. NM_001179411.1.

3D structure databases

ProteinModelPortalQ00916.
SMRQ00916. Positions 109-204.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34931. 82 interactions.
DIPDIP-663N.
IntActQ00916. 24 interactions.
MINTMINT-397124.
STRING4932.YIL061C.

Proteomic databases

MaxQBQ00916.
PaxDbQ00916.
PeptideAtlasQ00916.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL061C; YIL061C; YIL061C.
GeneID854749.
KEGGsce:YIL061C.

Organism-specific databases

CYGDYIL061c.
SGDS000001323. SNP1.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00530000063750.
HOGENOMHOG000000970.
KOK11093.
OMAEFMKDPY.
OrthoDBEOG7BW0WH.

Enzyme and pathway databases

BioCycYEAST:G3O-31329-MONOMER.

Gene expression databases

GenevestigatorQ00916.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977479.
PROQ00916.

Entry information

Entry nameRU17_YEAST
AccessionPrimary (citable) accession number: Q00916
Secondary accession number(s): D6VVM3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families