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Q00916

- RU17_YEAST

UniProt

Q00916 - RU17_YEAST

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Protein

U1 small nuclear ribonucleoprotein 70 kDa homolog

Gene

SNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in nuclear mRNA splicing.

GO - Molecular functioni

  1. mRNA binding Source: SGD
  2. nucleotide binding Source: InterPro
  3. U1 snRNA binding Source: SGD

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31329-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa homolog
Short name:
U1 70K
Short name:
U1 snRNP 70 kDa homolog
Short name:
U1-70K
Alternative name(s):
U1 small nuclear ribonucleoprotein SNP1
Short name:
U1 snRNP protein SNP1
Gene namesi
Name:SNP1
Ordered Locus Names:YIL061C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL061c.
SGDiS000001323. SNP1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. commitment complex Source: SGD
  2. U1 snRNP Source: SGD
  3. U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi18 – 9881Missing: Severely temperature-sensitive. Defective in pre-mRNA splicing. 1 PublicationAdd
BLAST
Mutagenesisi18 – 9376Missing: Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP. 1 PublicationAdd
BLAST
Mutagenesisi92 – 248157Missing: Associates with U1 snRNP. 2 PublicationsAdd
BLAST
Mutagenesisi148 – 1481K → L: No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152. 2 Publications
Mutagenesisi150 – 1501Y → T: No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152. 2 Publications
Mutagenesisi152 – 1521F → L: No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300U1 small nuclear ribonucleoprotein 70 kDa homologPRO_0000081886Add
BLAST

Proteomic databases

MaxQBiQ00916.
PaxDbiQ00916.
PeptideAtlasiQ00916.

Expressioni

Gene expression databases

GenevestigatoriQ00916.

Interactioni

Subunit structurei

Component of the spliceosome, where it is associated with snRNP U1. Binds stem loop I of U1 snRNA. Interacts with mRNA.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EXO84P382612EBI-724,EBI-21567
PRP8P333342EBI-724,EBI-465

Protein-protein interaction databases

BioGridi34931. 83 interactions.
DIPiDIP-663N.
IntActiQ00916. 24 interactions.
MINTiMINT-397124.
STRINGi4932.YIL061C.

Structurei

3D structure databases

ProteinModelPortaliQ00916.
SMRiQ00916. Positions 109-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 19892RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00530000063750.
HOGENOMiHOG000000970.
InParanoidiQ00916.
KOiK11093.
OMAiEFMKDPY.
OrthoDBiEOG7BW0WH.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00916-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYNLSKYPD DVSRLFKPRP PLSYKRPTDY PYAKRQTNPN ITGVANLLST
60 70 80 90 100
SLKHYMEEFP EGSPNNHLQR YEDIKLSKIK NAQLLDRRLQ NWNPNVDPHI
110 120 130 140 150
KDTDPYRTIF IGRLPYDLDE IELQKYFVKF GEIEKIRIVK DKITQKSKGY
160 170 180 190 200
AFIVFKDPIS SKMAFKEIGV HRGIQIKDRI CIVDIERGRT VKYFKPRRLG
210 220 230 240 250
GGLGGRGYSN RDSRLPGRFA SASTSNPAER NYAPRLPRRE TSSSAYSADR
260 270 280 290 300
YGSSTLDARY RGNRPLLSAA TPTAAVTSVY KSRNSRTRES QPAPKEAPDY
Length:300
Mass (Da):34,447
Last modified:April 1, 1993 - v1
Checksum:i7D9E47BE66FE1EB8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38060 Genomic DNA. Translation: CAA86162.1.
X59986 Genomic DNA. Translation: CAA42602.1.
AY558085 Genomic DNA. Translation: AAS56411.1.
BK006942 Genomic DNA. Translation: DAA08489.1.
PIRiS16815.
RefSeqiNP_012203.1. NM_001179411.1.

Genome annotation databases

EnsemblFungiiYIL061C; YIL061C; YIL061C.
GeneIDi854749.
KEGGisce:YIL061C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38060 Genomic DNA. Translation: CAA86162.1 .
X59986 Genomic DNA. Translation: CAA42602.1 .
AY558085 Genomic DNA. Translation: AAS56411.1 .
BK006942 Genomic DNA. Translation: DAA08489.1 .
PIRi S16815.
RefSeqi NP_012203.1. NM_001179411.1.

3D structure databases

ProteinModelPortali Q00916.
SMRi Q00916. Positions 109-204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34931. 83 interactions.
DIPi DIP-663N.
IntActi Q00916. 24 interactions.
MINTi MINT-397124.
STRINGi 4932.YIL061C.

Proteomic databases

MaxQBi Q00916.
PaxDbi Q00916.
PeptideAtlasi Q00916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL061C ; YIL061C ; YIL061C .
GeneIDi 854749.
KEGGi sce:YIL061C.

Organism-specific databases

CYGDi YIL061c.
SGDi S000001323. SNP1.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00530000063750.
HOGENOMi HOG000000970.
InParanoidi Q00916.
KOi K11093.
OMAi EFMKDPY.
OrthoDBi EOG7BW0WH.

Enzyme and pathway databases

BioCyci YEAST:G3O-31329-MONOMER.

Miscellaneous databases

NextBioi 977479.
PROi Q00916.

Gene expression databases

Genevestigatori Q00916.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast."
    Smith V., Barrell B.G.
    EMBO J. 10:2627-2634(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene."
    Kao H.-Y., Siliciano P.G.
    Nucleic Acids Res. 20:4009-4013(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: U1 RNA-BINDING.
  6. "The amino-terminal domain of yeast U1-70K is necessary and sufficient for function."
    Hilleren P.J., Kao H.-Y., Siliciano P.G.
    Mol. Cell. Biol. 15:6341-6350(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 18-PRO--PRO-98; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
  7. "The RRM domain is dispensable for yeast U1-70K function."
    Hilleren P.J., Kao H.-Y., Siliciano P.G.
    Nucleic Acids Symp. Ser. 33:244-247(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 18-PRO--ASN-93; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
  8. "Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry."
    Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M.
    Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
  9. "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
    Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
    RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
  10. "Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex."
    Zhang D., Rosbash M.
    Genes Dev. 13:581-592(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MRNA.
  11. "New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
    Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
    J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EXO84 AND PRP8.
  12. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRU17_YEAST
AccessioniPrimary (citable) accession number: Q00916
Secondary accession number(s): D6VVM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3