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Q00916

- RU17_YEAST

UniProt

Q00916 - RU17_YEAST

Protein

U1 small nuclear ribonucleoprotein 70 kDa homolog

Gene

SNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Involved in nuclear mRNA splicing.

    GO - Molecular functioni

    1. mRNA binding Source: SGD
    2. nucleotide binding Source: InterPro
    3. protein binding Source: IntAct
    4. U1 snRNA binding Source: SGD

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31329-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U1 small nuclear ribonucleoprotein 70 kDa homolog
    Short name:
    U1 70K
    Short name:
    U1 snRNP 70 kDa homolog
    Short name:
    U1-70K
    Alternative name(s):
    U1 small nuclear ribonucleoprotein SNP1
    Short name:
    U1 snRNP protein SNP1
    Gene namesi
    Name:SNP1
    Ordered Locus Names:YIL061C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL061c.
    SGDiS000001323. SNP1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. commitment complex Source: SGD
    2. U1 snRNP Source: SGD
    3. U2-type prespliceosome Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi18 – 9881Missing: Severely temperature-sensitive. Defective in pre-mRNA splicing. Add
    BLAST
    Mutagenesisi18 – 9376Missing: Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP. Add
    BLAST
    Mutagenesisi92 – 248157Missing: Associates with U1 snRNP. Add
    BLAST
    Mutagenesisi148 – 1481K → L: No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152. 2 Publications
    Mutagenesisi150 – 1501Y → T: No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152. 2 Publications
    Mutagenesisi152 – 1521F → L: No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300U1 small nuclear ribonucleoprotein 70 kDa homologPRO_0000081886Add
    BLAST

    Proteomic databases

    MaxQBiQ00916.
    PaxDbiQ00916.
    PeptideAtlasiQ00916.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00916.

    Interactioni

    Subunit structurei

    Component of the spliceosome, where it is associated with snRNP U1. Binds stem loop I of U1 snRNA. Interacts with mRNA.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EXO84P382612EBI-724,EBI-21567
    PRP8P333342EBI-724,EBI-465

    Protein-protein interaction databases

    BioGridi34931. 82 interactions.
    DIPiDIP-663N.
    IntActiQ00916. 24 interactions.
    MINTiMINT-397124.
    STRINGi4932.YIL061C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00916.
    SMRiQ00916. Positions 109-204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 19892RRMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00530000063750.
    HOGENOMiHOG000000970.
    KOiK11093.
    OMAiEFMKDPY.
    OrthoDBiEOG7BW0WH.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR022023. U1snRNP70_N.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    PF12220. U1snRNP70_N. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYNLSKYPD DVSRLFKPRP PLSYKRPTDY PYAKRQTNPN ITGVANLLST    50
    SLKHYMEEFP EGSPNNHLQR YEDIKLSKIK NAQLLDRRLQ NWNPNVDPHI 100
    KDTDPYRTIF IGRLPYDLDE IELQKYFVKF GEIEKIRIVK DKITQKSKGY 150
    AFIVFKDPIS SKMAFKEIGV HRGIQIKDRI CIVDIERGRT VKYFKPRRLG 200
    GGLGGRGYSN RDSRLPGRFA SASTSNPAER NYAPRLPRRE TSSSAYSADR 250
    YGSSTLDARY RGNRPLLSAA TPTAAVTSVY KSRNSRTRES QPAPKEAPDY 300
    Length:300
    Mass (Da):34,447
    Last modified:April 1, 1993 - v1
    Checksum:i7D9E47BE66FE1EB8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38060 Genomic DNA. Translation: CAA86162.1.
    X59986 Genomic DNA. Translation: CAA42602.1.
    AY558085 Genomic DNA. Translation: AAS56411.1.
    BK006942 Genomic DNA. Translation: DAA08489.1.
    PIRiS16815.
    RefSeqiNP_012203.1. NM_001179411.1.

    Genome annotation databases

    EnsemblFungiiYIL061C; YIL061C; YIL061C.
    GeneIDi854749.
    KEGGisce:YIL061C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38060 Genomic DNA. Translation: CAA86162.1 .
    X59986 Genomic DNA. Translation: CAA42602.1 .
    AY558085 Genomic DNA. Translation: AAS56411.1 .
    BK006942 Genomic DNA. Translation: DAA08489.1 .
    PIRi S16815.
    RefSeqi NP_012203.1. NM_001179411.1.

    3D structure databases

    ProteinModelPortali Q00916.
    SMRi Q00916. Positions 109-204.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34931. 82 interactions.
    DIPi DIP-663N.
    IntActi Q00916. 24 interactions.
    MINTi MINT-397124.
    STRINGi 4932.YIL061C.

    Proteomic databases

    MaxQBi Q00916.
    PaxDbi Q00916.
    PeptideAtlasi Q00916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL061C ; YIL061C ; YIL061C .
    GeneIDi 854749.
    KEGGi sce:YIL061C.

    Organism-specific databases

    CYGDi YIL061c.
    SGDi S000001323. SNP1.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00530000063750.
    HOGENOMi HOG000000970.
    KOi K11093.
    OMAi EFMKDPY.
    OrthoDBi EOG7BW0WH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31329-MONOMER.

    Miscellaneous databases

    NextBioi 977479.
    PROi Q00916.

    Gene expression databases

    Genevestigatori Q00916.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR022023. U1snRNP70_N.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    PF12220. U1snRNP70_N. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast."
      Smith V., Barrell B.G.
      EMBO J. 10:2627-2634(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene."
      Kao H.-Y., Siliciano P.G.
      Nucleic Acids Res. 20:4009-4013(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: U1 RNA-BINDING.
    6. "The amino-terminal domain of yeast U1-70K is necessary and sufficient for function."
      Hilleren P.J., Kao H.-Y., Siliciano P.G.
      Mol. Cell. Biol. 15:6341-6350(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 18-PRO--PRO-98; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
    7. "The RRM domain is dispensable for yeast U1-70K function."
      Hilleren P.J., Kao H.-Y., Siliciano P.G.
      Nucleic Acids Symp. Ser. 33:244-247(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 18-PRO--ASN-93; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
    8. "Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry."
      Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M.
      Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
    9. "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
      Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
      RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
    10. "Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex."
      Zhang D., Rosbash M.
      Genes Dev. 13:581-592(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MRNA.
    11. "New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing."
      Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W.
      J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EXO84 AND PRP8.
    12. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
      Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
      Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRU17_YEAST
    AccessioniPrimary (citable) accession number: Q00916
    Secondary accession number(s): D6VVM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 736 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3