Q00916 (RU17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa homolog
Short name=U1 70K
Short name=U1 snRNP 70 kDa homolog
Short name=U1-70K

Alternative name(s):
U1 small nuclear ribonucleoprotein SNP1
Short name=U1 snRNP protein SNP1

Gene names

Name:	SNP1
Ordered Locus Names:	YIL061C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nuclear mRNA splicing.
Subunit structure	Component of the spliceosome, where it is associated with snRNP U1. Binds stem loop I of U1 snRNA. Interacts with USA1. Interacts with mRNA. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 736 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from physical interaction Ref.10. Source: SGD
Cellular_component	U1 snRNP Inferred from direct assay Ref.9. Source: SGD U2-type prespliceosome Inferred from direct assay PubMed 16618970. Source: SGD commitment complex Inferred from physical interaction Ref.10. Source: SGD
Molecular_function	U1 snRNA binding Inferred from physical interaction Ref.5. Source: SGD mRNA binding Inferred from physical interaction Ref.10. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EXO84	P38261	2	EBI-724,EBI-21567
PRP8	P33334	3	EBI-724,EBI-465

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	U1 small nuclear ribonucleoprotein 70 kDa homolog		PRO_0000081886
Regions
	Domain	107 – 198	92	RRM
Experimental info
	Mutagenesis	18 – 98	81	Missing: Severely temperature-sensitive. Defective in pre-mRNA splicing. Ref.6
	Mutagenesis	18 – 93	76	Missing: Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP. Ref.7
	Mutagenesis	92 – 248	157	Missing: Associates with U1 snRNP. Ref.6 Ref.7
	Mutagenesis	148	1	K → L: No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152. Ref.6 Ref.7
	Mutagenesis	150	1	Y → T: No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152. Ref.6 Ref.7
	Mutagenesis	152	1	F → L: No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150. Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q00916 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 7D9E47BE66FE1EB8	FASTA	300	34,447
	10 20 30 40 50 60 MNYNLSKYPD DVSRLFKPRP PLSYKRPTDY PYAKRQTNPN ITGVANLLST SLKHYMEEFP 70 80 90 100 110 120 EGSPNNHLQR YEDIKLSKIK NAQLLDRRLQ NWNPNVDPHI KDTDPYRTIF IGRLPYDLDE 130 140 150 160 170 180 IELQKYFVKF GEIEKIRIVK DKITQKSKGY AFIVFKDPIS SKMAFKEIGV HRGIQIKDRI 190 200 210 220 230 240 CIVDIERGRT VKYFKPRRLG GGLGGRGYSN RDSRLPGRFA SASTSNPAER NYAPRLPRRE 250 260 270 280 290 300 TSSSAYSADR YGSSTLDARY RGNRPLLSAA TPTAAVTSVY KSRNSRTRES QPAPKEAPDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast." Smith V., Barrell B.G. EMBO J. 10:2627-2634(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene." Kao H.-Y., Siliciano P.G. Nucleic Acids Res. 20:4009-4013(1992) [PubMed] [Europe PMC] [Abstract] Cited for: U1 RNA-BINDING.
[6]	"The amino-terminal domain of yeast U1-70K is necessary and sufficient for function." Hilleren P.J., Kao H.-Y., Siliciano P.G. Mol. Cell. Biol. 15:6341-6350(1995) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF 18-PRO--PRO-98; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
[7]	"The RRM domain is dispensable for yeast U1-70K function." Hilleren P.J., Kao H.-Y., Siliciano P.G. Nucleic Acids Symp. Ser. 33:244-247(1995) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF 18-PRO--ASN-93; 92-TRP--ALA-248; LYS-148; TYR-150 AND PHE-152.
[8]	"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry." Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M. Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[9]	"A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins." Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P. RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[10]	"Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex." Zhang D., Rosbash M. Genes Dev. 13:581-592(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MRNA.
[11]	"New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing." Awasthi S., Palmer R., Castro M., Mobarak C.D., Ruby S.W. J. Biol. Chem. 276:31004-31015(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EXO84; PRP8 AND USA1.
[12]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38060 Genomic DNA. Translation: CAA86162.1. X59986 Genomic DNA. Translation: CAA42602.1. AY558085 Genomic DNA. Translation: AAS56411.1. BK006942 Genomic DNA. Translation: DAA08489.1.
PIR	S16815.
RefSeq	NP_012203.1. NM_001179411.1.
3D structure databases
ProteinModelPortal	Q00916.
SMR	Q00916. Positions 104-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-663N.
IntAct	Q00916. 39 interactions.
MINT	MINT-397124.
STRING	4932.YIL061C.
Proteomic databases
PaxDb	Q00916.
PeptideAtlas	Q00916.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL061C; YIL061C; YIL061C.
GeneID	854749.
KEGG	sce:YIL061C.
Organism-specific databases
CYGD	YIL061c.
SGD	S000001323. SNP1.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00530000063750.
HOGENOM	HOG000000970.
KO	K11093.
OMA	ETSSSAY.
OrthoDB	EOG4HMNKR.
Gene expression databases
Genevestigator	Q00916.
GermOnline	YIL061C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR022023. U1snRNP70_N. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. PF12220. U1snRNP70_N. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977479.

Entry information

Entry name

RU17_YEAST

Accession

Primary (citable) accession number: Q00916
Secondary accession number(s): D6VVM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program