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Q00899

- TYY1_MOUSE

UniProt

Q00899 - TYY1_MOUSE

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Protein

Transcriptional repressor protein YY1

Gene

Yy1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. Binds to the upstream conserved region (UCR) (5'-CGCCATTTT-3') of Moloney murine leukemia virus (MuLV). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Involved in spermatogenesis and may play a role in meiotic DNA double-strand break repair.3 Publications
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi298 – 2981Zinc 1By similarity
Metal bindingi303 – 3031Zinc 1By similarity
Metal bindingi316 – 3161Zinc 1By similarity
Metal bindingi320 – 3201Zinc 1By similarity
Metal bindingi327 – 3271Zinc 2By similarity
Metal bindingi330 – 3301Zinc 2By similarity
Metal bindingi343 – 3431Zinc 2By similarity
Metal bindingi347 – 3471Zinc 2By similarity
Metal bindingi355 – 3551Zinc 3By similarity
Metal bindingi360 – 3601Zinc 3By similarity
Metal bindingi373 – 3731Zinc 3By similarity
Metal bindingi377 – 3771Zinc 3By similarity
Metal bindingi385 – 3851Zinc 4By similarity
Metal bindingi390 – 3901Zinc 4By similarity
Metal bindingi403 – 4031Zinc 4By similarity
Metal bindingi407 – 4071Zinc 4By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri296 – 32025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri353 – 37725C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri383 – 40725C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. four-way junction DNA binding Source: Ensembl
  3. metal ion binding Source: UniProtKB-KW
  4. RNA binding Source: MGI
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
  6. sequence-specific DNA binding transcription factor activity Source: MGI
  7. transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. camera-type eye morphogenesis Source: MGI
  3. cell differentiation Source: UniProtKB-KW
  4. cellular response to DNA damage stimulus Source: UniProtKB
  5. cellular response to UV Source: Ensembl
  6. chromosome organization Source: UniProtKB
  7. double-strand break repair via homologous recombination Source: Ensembl
  8. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  9. positive regulation of gene expression Source: MGI
  10. regulation of transcription, DNA-templated Source: MGI
  11. response to prostaglandin F Source: Ensembl
  12. response to UV-C Source: UniProtKB
  13. RNA localization Source: MGI
  14. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, DNA damage, DNA recombination, DNA repair, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor protein YY1
Alternative name(s):
Delta transcription factor
NF-E1
UCR-motif DNA-binding protein
Yin and yang 1
Short name:
YY-1
Gene namesi
Name:Yy1
Synonyms:Ucrbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:99150. Yy1.

Subcellular locationi

Nucleus matrix 1 Publication
Note: Associated with the nuclear matrix. In testis, localized to heterochromatin of spermatocytes.

GO - Cellular componenti

  1. Ino80 complex Source: Ensembl
  2. nucleus Source: MGI
  3. PcG protein complex Source: MGI
  4. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Spermatocytes have a significant decrease in the global level of the heterochromatin markers and increase in the chromosomal double-strand break (DSB) signals at the leptotene/zygotene stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Transcriptional repressor protein YY1PRO_0000047191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201PhosphoserineBy similarity
Modified residuei247 – 2471PhosphoserineBy similarity
Modified residuei378 – 3781PhosphothreonineBy similarity

Post-translational modificationi

Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ00899.
PaxDbiQ00899.
PRIDEiQ00899.

PTM databases

PhosphoSiteiQ00899.

Expressioni

Tissue specificityi

Expressed in ovary and, at lower levels, in testis.1 Publication

Developmental stagei

At 7.5 dpc, highly expressed in the ectoplacental cone and, at lower levels, in the embryonic and extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and yolk sac, and, at lower levels, in brain and heart.1 Publication

Gene expression databases

BgeeiQ00899.
CleanExiMM_YY1.
GenevestigatoriQ00899.

Interactioni

Subunit structurei

Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex (By similarity). Found in a complex with SMAD1 and SMAD4. Interacts with SFMBT2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Smc4Q8CG472EBI-6921536,EBI-6921575

Protein-protein interaction databases

BioGridi204624. 15 interactions.
DIPiDIP-59285N.
IntActiQ00899. 5 interactions.
MINTiMINT-4138987.
STRINGi10090.ENSMUSP00000021692.

Structurei

3D structure databases

ProteinModelPortaliQ00899.
SMRiQ00899. Positions 295-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 170170Interaction with the SMAD1/SMAD4 complexBy similarityAdd
BLAST
Regioni257 – 34185Involved in nuclear matrix associationBy similarityAdd
BLAST
Regioni295 – 414120Binding to DNABy similarityAdd
BLAST
Regioni333 – 37139Involved in repression of activated transcriptionBy similarityAdd
BLAST
Regioni371 – 39727Involved in masking transactivation domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 5311Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi54 – 7017Gly-richAdd
BLAST
Compositional biasi71 – 8212Poly-HisAdd
BLAST
Compositional biasi161 – 17010Gly/Ser-rich

Sequence similaritiesi

Belongs to the YY transcription factor family.Curated
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri296 – 32025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri353 – 37725C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri383 – 40725C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00770000120559.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiQ00899.
KOiK09201.
OMAiDIDHESV.
OrthoDBiEOG769ZJD.
PhylomeDBiQ00899.
TreeFamiTF106493.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PIRSFiPIRSF037113. TF_Yin_yang. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00899-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASGDTLYIA TDGSEMPAEI VELHEIEVET IPVETIETTV VGEEEEEDDD
60 70 80 90 100
DEDGGGGDHG GGGGGHGHAG HHHHHHHHHH HHPPMIALQP LVTDDPTQVH
110 120 130 140 150
HHQEVILVQT REEVVGGDDS DGLRAEDGFE DQILIPVPAP AGGDDDYIEQ
160 170 180 190 200
TLVTVAAAGK SGGGASSGGG RVKKGGGKKS GKKSYLGGGA GAAGGGGADP
210 220 230 240 250
GNKKWEQKQV QIKTLEGEFS VTMWSSDEKK DIDHETVVEE QIIGENSPPD
260 270 280 290 300
YSEYMTGKKL PPGGIPGIDL SDPKQLAEFA RMKPRKIKED DAPRTIACPH
310 320 330 340 350
KGCTKMFRDN SAMRKHLHTH GPRVHVCAEC GKAFVESSKL KRHQLVHTGE
360 370 380 390 400
KPFQCTFEGC GKRFSLDFNL RTHVRIHTGD RPYVCPFDGC NKKFAQSTNL
410
KSHILTHAKA KNNQ
Length:414
Mass (Da):44,717
Last modified:November 1, 1995 - v1
Checksum:iC012378288E984F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191F → S in AAA37521. (PubMed:1946404)Curated
Sequence conflicti375 – 3751R → G in AAA37521. (PubMed:1946404)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73963 mRNA. Translation: AAA40522.1.
L13968
, L13969, L13965, L13966, L13967 Genomic DNA. Translation: AAA40477.1.
M74590 mRNA. Translation: AAA37521.1.
BC055899 mRNA. Translation: AAH55899.1.
CCDSiCCDS26163.1.
PIRiA48273.
RefSeqiNP_033563.2. NM_009537.3.
UniGeneiMm.3868.
Mm.458511.

Genome annotation databases

EnsembliENSMUST00000021692; ENSMUSP00000021692; ENSMUSG00000021264.
GeneIDi22632.
KEGGimmu:22632.
UCSCiuc007pac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73963 mRNA. Translation: AAA40522.1 .
L13968
, L13969 , L13965 , L13966 , L13967 Genomic DNA. Translation: AAA40477.1 .
M74590 mRNA. Translation: AAA37521.1 .
BC055899 mRNA. Translation: AAH55899.1 .
CCDSi CCDS26163.1.
PIRi A48273.
RefSeqi NP_033563.2. NM_009537.3.
UniGenei Mm.3868.
Mm.458511.

3D structure databases

ProteinModelPortali Q00899.
SMRi Q00899. Positions 295-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204624. 15 interactions.
DIPi DIP-59285N.
IntActi Q00899. 5 interactions.
MINTi MINT-4138987.
STRINGi 10090.ENSMUSP00000021692.

PTM databases

PhosphoSitei Q00899.

Proteomic databases

MaxQBi Q00899.
PaxDbi Q00899.
PRIDEi Q00899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021692 ; ENSMUSP00000021692 ; ENSMUSG00000021264 .
GeneIDi 22632.
KEGGi mmu:22632.
UCSCi uc007pac.1. mouse.

Organism-specific databases

CTDi 7528.
MGIi MGI:99150. Yy1.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00770000120559.
HOGENOMi HOG000008232.
HOVERGENi HBG006823.
InParanoidi Q00899.
KOi K09201.
OMAi DIDHESV.
OrthoDBi EOG769ZJD.
PhylomeDBi Q00899.
TreeFami TF106493.

Miscellaneous databases

ChiTaRSi Yy1. mouse.
NextBioi 303007.
PROi Q00899.
SOURCEi Search...

Gene expression databases

Bgeei Q00899.
CleanExi MM_YY1.
Genevestigatori Q00899.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
PIRSFi PIRSF037113. TF_Yin_yang. 1 hit.
SMARTi SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a negative transcription factor that binds to the upstream conserved region of Moloney murine leukemia virus."
    Flanagan J.R., Becker K.G., Ennist D.L., Gleason S.L., Driggers P.H., Levi B.-Z., Appella E., Ozato K.
    Mol. Cell. Biol. 12:38-44(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the mouse gene that encodes the delta/YY1/NF-E1/UCRBP transcription factor."
    Safrany G., Perry R.P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5559-5563(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein."
    Hariharan N., Kelley D.E., Perry R.P.
    Proc. Natl. Acad. Sci. U.S.A. 88:9799-9803(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer."
    Lee K.H., Evans S., Ruan T.Y., Lassar A.B.
    Development 131:4709-4723(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, IDENTIFICATION IN A COMPLEX WITH SMAD1 AND SMAD4.
  6. "A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair."
    Wu S., Shi Y., Mulligan P., Gay F., Landry J., Liu H., Lu J., Qi H.H., Wang W., Nickoloff J.A., Wu C., Shi Y.
    Nat. Struct. Mol. Biol. 14:1165-1172(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DNA REPAIR.
  7. "The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues."
    Kuzmin A., Han Z., Golding M.C., Mann M.R., Latham K.E., Varmuza S.
    Gene Expr. Patterns 8:107-116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SFMBT2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis."
    Wu S., Hu Y.C., Liu H., Shi Y.
    Mol. Cell. Biol. 29:6245-6256(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPERMATOGENESIS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTYY1_MOUSE
AccessioniPrimary (citable) accession number: Q00899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3