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Q00899

- TYY1_MOUSE

UniProt

Q00899 - TYY1_MOUSE

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Protein

Transcriptional repressor protein YY1

Gene
Yy1, Ucrbp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. Binds to the upstream conserved region (UCR) (5'-CGCCATTTT-3') of Moloney murine leukemia virus (MuLV). Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Involved in spermatogenesis and may play a role in meiotic DNA double-strand break repair.2 Publications
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements By similarity.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi298 – 2981Zinc 1 By similarity
Metal bindingi303 – 3031Zinc 1 By similarity
Metal bindingi316 – 3161Zinc 1 By similarity
Metal bindingi320 – 3201Zinc 1 By similarity
Metal bindingi327 – 3271Zinc 2 By similarity
Metal bindingi330 – 3301Zinc 2 By similarity
Metal bindingi343 – 3431Zinc 2 By similarity
Metal bindingi347 – 3471Zinc 2 By similarity
Metal bindingi355 – 3551Zinc 3 By similarity
Metal bindingi360 – 3601Zinc 3 By similarity
Metal bindingi373 – 3731Zinc 3 By similarity
Metal bindingi377 – 3771Zinc 3 By similarity
Metal bindingi385 – 3851Zinc 4 By similarity
Metal bindingi390 – 3901Zinc 4 By similarity
Metal bindingi403 – 4031Zinc 4 By similarity
Metal bindingi407 – 4071Zinc 4 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri296 – 32025C2H2-type 1Add
BLAST
Zinc fingeri325 – 34723C2H2-type 2Add
BLAST
Zinc fingeri353 – 37725C2H2-type 3Add
BLAST
Zinc fingeri383 – 40725C2H2-type 4Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. four-way junction DNA binding Source: Ensembl
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: IntAct
  5. RNA binding Source: MGI
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
  7. sequence-specific DNA binding transcription factor activity Source: MGI
  8. transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. camera-type eye morphogenesis Source: MGI
  3. cell differentiation Source: UniProtKB-KW
  4. cellular response to DNA damage stimulus Source: UniProtKB
  5. cellular response to UV Source: Ensembl
  6. chromosome organization Source: UniProtKB
  7. double-strand break repair via homologous recombination Source: Ensembl
  8. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  9. regulation of transcription, DNA-templated Source: MGI
  10. response to prostaglandin F Source: Ensembl
  11. response to UV-C Source: UniProtKB
  12. RNA localization Source: MGI
  13. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, DNA damage, DNA recombination, DNA repair, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor protein YY1
Alternative name(s):
Delta transcription factor
NF-E1
UCR-motif DNA-binding protein
Yin and yang 1
Short name:
YY-1
Gene namesi
Name:Yy1
Synonyms:Ucrbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:99150. Yy1.

Subcellular locationi

Nucleus matrix
Note: Associated with the nuclear matrix. In testis, localized to heterochromatin of spermatocytes.1 Publication

GO - Cellular componenti

  1. Ino80 complex Source: Ensembl
  2. nuclear matrix Source: UniProtKB-SubCell
  3. nucleus Source: MGI
  4. PcG protein complex Source: MGI
  5. plasma membrane Source: Ensembl
  6. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Spermatocytes have a significant decrease in the global level of the heterochromatin markers and increase in the chromosomal double-strand break (DSB) signals at the leptotene/zygotene stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Transcriptional repressor protein YY1PRO_0000047191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine By similarity
Modified residuei247 – 2471Phosphoserine By similarity
Modified residuei378 – 3781Phosphothreonine By similarity

Post-translational modificationi

Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites By similarity.
Ubiquitinated By similarity.

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ00899.
PaxDbiQ00899.
PRIDEiQ00899.

PTM databases

PhosphoSiteiQ00899.

Expressioni

Tissue specificityi

Expressed in ovary and, at lower levels, in testis.1 Publication

Developmental stagei

At 7.5 dpc, highly expressed in the ectoplacental cone and, at lower levels, in the embryonic and extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and yolk sac, and, at lower levels, in brain and heart.1 Publication

Gene expression databases

BgeeiQ00899.
CleanExiMM_YY1.
GenevestigatoriQ00899.

Interactioni

Subunit structurei

Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex By similarity. Interacts with SFMBT2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Smc4Q8CG472EBI-6921536,EBI-6921575

Protein-protein interaction databases

BioGridi204624. 12 interactions.
DIPiDIP-59285N.
IntActiQ00899. 5 interactions.
MINTiMINT-4138987.
STRINGi10090.ENSMUSP00000021692.

Structurei

3D structure databases

ProteinModelPortaliQ00899.
SMRiQ00899. Positions 295-408.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 34185Involved in nuclear matrix association By similarityAdd
BLAST
Regioni333 – 37139Involved in repression of activated transcription By similarityAdd
BLAST
Regioni371 – 39727Involved in masking transactivation domain By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 5311Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi54 – 7017Gly-richAdd
BLAST
Compositional biasi71 – 8212Poly-HisAdd
BLAST
Compositional biasi161 – 17010Gly/Ser-rich

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00730000110826.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiQ00899.
KOiK09201.
OMAiDIDHESV.
OrthoDBiEOG769ZJD.
PhylomeDBiQ00899.
TreeFamiTF106493.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PIRSFiPIRSF037113. TF_Yin_yang. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00899-1 [UniParc]FASTAAdd to Basket

« Hide

MASGDTLYIA TDGSEMPAEI VELHEIEVET IPVETIETTV VGEEEEEDDD    50
DEDGGGGDHG GGGGGHGHAG HHHHHHHHHH HHPPMIALQP LVTDDPTQVH 100
HHQEVILVQT REEVVGGDDS DGLRAEDGFE DQILIPVPAP AGGDDDYIEQ 150
TLVTVAAAGK SGGGASSGGG RVKKGGGKKS GKKSYLGGGA GAAGGGGADP 200
GNKKWEQKQV QIKTLEGEFS VTMWSSDEKK DIDHETVVEE QIIGENSPPD 250
YSEYMTGKKL PPGGIPGIDL SDPKQLAEFA RMKPRKIKED DAPRTIACPH 300
KGCTKMFRDN SAMRKHLHTH GPRVHVCAEC GKAFVESSKL KRHQLVHTGE 350
KPFQCTFEGC GKRFSLDFNL RTHVRIHTGD RPYVCPFDGC NKKFAQSTNL 400
KSHILTHAKA KNNQ 414
Length:414
Mass (Da):44,717
Last modified:November 1, 1995 - v1
Checksum:iC012378288E984F9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191F → S in AAA37521. 1 Publication
Sequence conflicti375 – 3751R → G in AAA37521. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73963 mRNA. Translation: AAA40522.1.
L13968
, L13969, L13965, L13966, L13967 Genomic DNA. Translation: AAA40477.1.
M74590 mRNA. Translation: AAA37521.1.
BC055899 mRNA. Translation: AAH55899.1.
CCDSiCCDS26163.1.
PIRiA48273.
RefSeqiNP_033563.2. NM_009537.3.
UniGeneiMm.3868.
Mm.458511.

Genome annotation databases

EnsembliENSMUST00000021692; ENSMUSP00000021692; ENSMUSG00000021264.
GeneIDi22632.
KEGGimmu:22632.
UCSCiuc007pac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73963 mRNA. Translation: AAA40522.1 .
L13968
, L13969 , L13965 , L13966 , L13967 Genomic DNA. Translation: AAA40477.1 .
M74590 mRNA. Translation: AAA37521.1 .
BC055899 mRNA. Translation: AAH55899.1 .
CCDSi CCDS26163.1.
PIRi A48273.
RefSeqi NP_033563.2. NM_009537.3.
UniGenei Mm.3868.
Mm.458511.

3D structure databases

ProteinModelPortali Q00899.
SMRi Q00899. Positions 295-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204624. 12 interactions.
DIPi DIP-59285N.
IntActi Q00899. 5 interactions.
MINTi MINT-4138987.
STRINGi 10090.ENSMUSP00000021692.

PTM databases

PhosphoSitei Q00899.

Proteomic databases

MaxQBi Q00899.
PaxDbi Q00899.
PRIDEi Q00899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021692 ; ENSMUSP00000021692 ; ENSMUSG00000021264 .
GeneIDi 22632.
KEGGi mmu:22632.
UCSCi uc007pac.1. mouse.

Organism-specific databases

CTDi 7528.
MGIi MGI:99150. Yy1.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00730000110826.
HOGENOMi HOG000008232.
HOVERGENi HBG006823.
InParanoidi Q00899.
KOi K09201.
OMAi DIDHESV.
OrthoDBi EOG769ZJD.
PhylomeDBi Q00899.
TreeFami TF106493.

Miscellaneous databases

ChiTaRSi YY1. mouse.
NextBioi 303007.
PROi Q00899.
SOURCEi Search...

Gene expression databases

Bgeei Q00899.
CleanExi MM_YY1.
Genevestigatori Q00899.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
PIRSFi PIRSF037113. TF_Yin_yang. 1 hit.
SMARTi SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a negative transcription factor that binds to the upstream conserved region of Moloney murine leukemia virus."
    Flanagan J.R., Becker K.G., Ennist D.L., Gleason S.L., Driggers P.H., Levi B.-Z., Appella E., Ozato K.
    Mol. Cell. Biol. 12:38-44(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the mouse gene that encodes the delta/YY1/NF-E1/UCRBP transcription factor."
    Safrany G., Perry R.P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5559-5563(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein."
    Hariharan N., Kelley D.E., Perry R.P.
    Proc. Natl. Acad. Sci. U.S.A. 88:9799-9803(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair."
    Wu S., Shi Y., Mulligan P., Gay F., Landry J., Liu H., Lu J., Qi H.H., Wang W., Nickoloff J.A., Wu C., Shi Y.
    Nat. Struct. Mol. Biol. 14:1165-1172(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DNA REPAIR.
  6. "The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues."
    Kuzmin A., Han Z., Golding M.C., Mann M.R., Latham K.E., Varmuza S.
    Gene Expr. Patterns 8:107-116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SFMBT2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis."
    Wu S., Hu Y.C., Liu H., Shi Y.
    Mol. Cell. Biol. 29:6245-6256(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPERMATOGENESIS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTYY1_MOUSE
AccessioniPrimary (citable) accession number: Q00899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi