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Q00899

- TYY1_MOUSE

UniProt

Q00899 - TYY1_MOUSE

Protein

Transcriptional repressor protein YY1

Gene

Yy1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. Binds to the upstream conserved region (UCR) (5'-CGCCATTTT-3') of Moloney murine leukemia virus (MuLV). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Involved in spermatogenesis and may play a role in meiotic DNA double-strand break repair.3 Publications
    Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi298 – 2981Zinc 1By similarity
    Metal bindingi303 – 3031Zinc 1By similarity
    Metal bindingi316 – 3161Zinc 1By similarity
    Metal bindingi320 – 3201Zinc 1By similarity
    Metal bindingi327 – 3271Zinc 2By similarity
    Metal bindingi330 – 3301Zinc 2By similarity
    Metal bindingi343 – 3431Zinc 2By similarity
    Metal bindingi347 – 3471Zinc 2By similarity
    Metal bindingi355 – 3551Zinc 3By similarity
    Metal bindingi360 – 3601Zinc 3By similarity
    Metal bindingi373 – 3731Zinc 3By similarity
    Metal bindingi377 – 3771Zinc 3By similarity
    Metal bindingi385 – 3851Zinc 4By similarity
    Metal bindingi390 – 3901Zinc 4By similarity
    Metal bindingi403 – 4031Zinc 4By similarity
    Metal bindingi407 – 4071Zinc 4By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri296 – 32025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri353 – 37725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri383 – 40725C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. four-way junction DNA binding Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. RNA binding Source: MGI
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
    7. sequence-specific DNA binding transcription factor activity Source: MGI
    8. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. anterior/posterior pattern specification Source: MGI
    2. camera-type eye morphogenesis Source: MGI
    3. cell differentiation Source: UniProtKB-KW
    4. cellular response to DNA damage stimulus Source: UniProtKB
    5. cellular response to UV Source: Ensembl
    6. chromosome organization Source: UniProtKB
    7. double-strand break repair via homologous recombination Source: Ensembl
    8. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    9. regulation of transcription, DNA-templated Source: MGI
    10. response to prostaglandin F Source: Ensembl
    11. response to UV-C Source: UniProtKB
    12. RNA localization Source: MGI
    13. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Differentiation, DNA damage, DNA recombination, DNA repair, Spermatogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional repressor protein YY1
    Alternative name(s):
    Delta transcription factor
    NF-E1
    UCR-motif DNA-binding protein
    Yin and yang 1
    Short name:
    YY-1
    Gene namesi
    Name:Yy1
    Synonyms:Ucrbp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:99150. Yy1.

    Subcellular locationi

    Nucleus matrix 1 Publication
    Note: Associated with the nuclear matrix. In testis, localized to heterochromatin of spermatocytes.

    GO - Cellular componenti

    1. Ino80 complex Source: Ensembl
    2. nuclear matrix Source: UniProtKB-SubCell
    3. nucleus Source: MGI
    4. PcG protein complex Source: MGI
    5. plasma membrane Source: Ensembl
    6. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Spermatocytes have a significant decrease in the global level of the heterochromatin markers and increase in the chromosomal double-strand break (DSB) signals at the leptotene/zygotene stages.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Transcriptional repressor protein YY1PRO_0000047191Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei120 – 1201PhosphoserineBy similarity
    Modified residuei247 – 2471PhosphoserineBy similarity
    Modified residuei378 – 3781PhosphothreonineBy similarity

    Post-translational modificationi

    Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.By similarity
    Ubiquitinated.By similarity

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ00899.
    PaxDbiQ00899.
    PRIDEiQ00899.

    PTM databases

    PhosphoSiteiQ00899.

    Expressioni

    Tissue specificityi

    Expressed in ovary and, at lower levels, in testis.1 Publication

    Developmental stagei

    At 7.5 dpc, highly expressed in the ectoplacental cone and, at lower levels, in the embryonic and extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and yolk sac, and, at lower levels, in brain and heart.1 Publication

    Gene expression databases

    BgeeiQ00899.
    CleanExiMM_YY1.
    GenevestigatoriQ00899.

    Interactioni

    Subunit structurei

    Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex By similarity. Found in a complex with SMAD1 and SMAD4. Interacts with SFMBT2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Smc4Q8CG472EBI-6921536,EBI-6921575

    Protein-protein interaction databases

    BioGridi204624. 12 interactions.
    DIPiDIP-59285N.
    IntActiQ00899. 5 interactions.
    MINTiMINT-4138987.
    STRINGi10090.ENSMUSP00000021692.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00899.
    SMRiQ00899. Positions 295-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 170170Interaction with the SMAD1/SMAD4 complexBy similarityAdd
    BLAST
    Regioni257 – 34185Involved in nuclear matrix associationBy similarityAdd
    BLAST
    Regioni295 – 414120Binding to DNABy similarityAdd
    BLAST
    Regioni333 – 37139Involved in repression of activated transcriptionBy similarityAdd
    BLAST
    Regioni371 – 39727Involved in masking transactivation domainBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi43 – 5311Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi54 – 7017Gly-richAdd
    BLAST
    Compositional biasi71 – 8212Poly-HisAdd
    BLAST
    Compositional biasi161 – 17010Gly/Ser-rich

    Sequence similaritiesi

    Belongs to the YY transcription factor family.Curated
    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri296 – 32025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri325 – 34723C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri353 – 37725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri383 – 40725C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00730000110826.
    HOGENOMiHOG000008232.
    HOVERGENiHBG006823.
    InParanoidiQ00899.
    KOiK09201.
    OMAiDIDHESV.
    OrthoDBiEOG769ZJD.
    PhylomeDBiQ00899.
    TreeFamiTF106493.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR017114. TF_Yin_yang.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PIRSFiPIRSF037113. TF_Yin_yang. 1 hit.
    SMARTiSM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00899-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGDTLYIA TDGSEMPAEI VELHEIEVET IPVETIETTV VGEEEEEDDD    50
    DEDGGGGDHG GGGGGHGHAG HHHHHHHHHH HHPPMIALQP LVTDDPTQVH 100
    HHQEVILVQT REEVVGGDDS DGLRAEDGFE DQILIPVPAP AGGDDDYIEQ 150
    TLVTVAAAGK SGGGASSGGG RVKKGGGKKS GKKSYLGGGA GAAGGGGADP 200
    GNKKWEQKQV QIKTLEGEFS VTMWSSDEKK DIDHETVVEE QIIGENSPPD 250
    YSEYMTGKKL PPGGIPGIDL SDPKQLAEFA RMKPRKIKED DAPRTIACPH 300
    KGCTKMFRDN SAMRKHLHTH GPRVHVCAEC GKAFVESSKL KRHQLVHTGE 350
    KPFQCTFEGC GKRFSLDFNL RTHVRIHTGD RPYVCPFDGC NKKFAQSTNL 400
    KSHILTHAKA KNNQ 414
    Length:414
    Mass (Da):44,717
    Last modified:November 1, 1995 - v1
    Checksum:iC012378288E984F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191F → S in AAA37521. (PubMed:1946404)Curated
    Sequence conflicti375 – 3751R → G in AAA37521. (PubMed:1946404)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73963 mRNA. Translation: AAA40522.1.
    L13968
    , L13969, L13965, L13966, L13967 Genomic DNA. Translation: AAA40477.1.
    M74590 mRNA. Translation: AAA37521.1.
    BC055899 mRNA. Translation: AAH55899.1.
    CCDSiCCDS26163.1.
    PIRiA48273.
    RefSeqiNP_033563.2. NM_009537.3.
    UniGeneiMm.3868.
    Mm.458511.

    Genome annotation databases

    EnsembliENSMUST00000021692; ENSMUSP00000021692; ENSMUSG00000021264.
    GeneIDi22632.
    KEGGimmu:22632.
    UCSCiuc007pac.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73963 mRNA. Translation: AAA40522.1 .
    L13968
    , L13969 , L13965 , L13966 , L13967 Genomic DNA. Translation: AAA40477.1 .
    M74590 mRNA. Translation: AAA37521.1 .
    BC055899 mRNA. Translation: AAH55899.1 .
    CCDSi CCDS26163.1.
    PIRi A48273.
    RefSeqi NP_033563.2. NM_009537.3.
    UniGenei Mm.3868.
    Mm.458511.

    3D structure databases

    ProteinModelPortali Q00899.
    SMRi Q00899. Positions 295-408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204624. 12 interactions.
    DIPi DIP-59285N.
    IntActi Q00899. 5 interactions.
    MINTi MINT-4138987.
    STRINGi 10090.ENSMUSP00000021692.

    PTM databases

    PhosphoSitei Q00899.

    Proteomic databases

    MaxQBi Q00899.
    PaxDbi Q00899.
    PRIDEi Q00899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021692 ; ENSMUSP00000021692 ; ENSMUSG00000021264 .
    GeneIDi 22632.
    KEGGi mmu:22632.
    UCSCi uc007pac.1. mouse.

    Organism-specific databases

    CTDi 7528.
    MGIi MGI:99150. Yy1.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00730000110826.
    HOGENOMi HOG000008232.
    HOVERGENi HBG006823.
    InParanoidi Q00899.
    KOi K09201.
    OMAi DIDHESV.
    OrthoDBi EOG769ZJD.
    PhylomeDBi Q00899.
    TreeFami TF106493.

    Miscellaneous databases

    ChiTaRSi YY1. mouse.
    NextBioi 303007.
    PROi Q00899.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q00899.
    CleanExi MM_YY1.
    Genevestigatori Q00899.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR017114. TF_Yin_yang.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    PIRSFi PIRSF037113. TF_Yin_yang. 1 hit.
    SMARTi SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a negative transcription factor that binds to the upstream conserved region of Moloney murine leukemia virus."
      Flanagan J.R., Becker K.G., Ennist D.L., Gleason S.L., Driggers P.H., Levi B.-Z., Appella E., Ozato K.
      Mol. Cell. Biol. 12:38-44(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of the mouse gene that encodes the delta/YY1/NF-E1/UCRBP transcription factor."
      Safrany G., Perry R.P.
      Proc. Natl. Acad. Sci. U.S.A. 90:5559-5563(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    3. "Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein."
      Hariharan N., Kelley D.E., Perry R.P.
      Proc. Natl. Acad. Sci. U.S.A. 88:9799-9803(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer."
      Lee K.H., Evans S., Ruan T.Y., Lassar A.B.
      Development 131:4709-4723(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, IDENTIFICATION IN A COMPLEX WITH SMAD1 AND SMAD4.
    6. "A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair."
      Wu S., Shi Y., Mulligan P., Gay F., Landry J., Liu H., Lu J., Qi H.H., Wang W., Nickoloff J.A., Wu C., Shi Y.
      Nat. Struct. Mol. Biol. 14:1165-1172(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DNA REPAIR.
    7. "The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues."
      Kuzmin A., Han Z., Golding M.C., Mann M.R., Latham K.E., Varmuza S.
      Gene Expr. Patterns 8:107-116(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SFMBT2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    8. "Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis."
      Wu S., Hu Y.C., Liu H., Shi Y.
      Mol. Cell. Biol. 29:6245-6256(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SPERMATOGENESIS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiTYY1_MOUSE
    AccessioniPrimary (citable) accession number: Q00899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3