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Protein

Transcriptional repressor protein YY1

Gene

Yy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. Binds to the upstream conserved region (UCR) (5'-CGCCATTTT-3') of Moloney murine leukemia virus (MuLV). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Involved in spermatogenesis and may play a role in meiotic DNA double-strand break repair.3 Publications
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298Zinc 1By similarity1
Metal bindingi303Zinc 1By similarity1
Metal bindingi316Zinc 1By similarity1
Metal bindingi320Zinc 1By similarity1
Metal bindingi327Zinc 2By similarity1
Metal bindingi330Zinc 2By similarity1
Metal bindingi343Zinc 2By similarity1
Metal bindingi347Zinc 2By similarity1
Metal bindingi355Zinc 3By similarity1
Metal bindingi360Zinc 3By similarity1
Metal bindingi373Zinc 3By similarity1
Metal bindingi377Zinc 3By similarity1
Metal bindingi385Zinc 4By similarity1
Metal bindingi390Zinc 4By similarity1
Metal bindingi403Zinc 4By similarity1
Metal bindingi407Zinc 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri296 – 320C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri325 – 347C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri353 – 377C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri383 – 407C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • camera-type eye morphogenesis Source: MGI
  • cell differentiation Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: UniProtKB
  • cellular response to interleukin-1 Source: Ensembl
  • cellular response to UV Source: MGI
  • chromosome organization Source: UniProtKB
  • double-strand break repair via homologous recombination Source: MGI
  • negative regulation of gene expression Source: MGI
  • negative regulation of pri-miRNA transcription from RNA polymerase II promoter Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of gene expression Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • regulation of transcription, DNA-templated Source: MGI
  • response to prostaglandin F Source: Ensembl
  • response to UV-C Source: UniProtKB
  • RNA localization Source: MGI
  • spermatogenesis Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, DNA damage, DNA recombination, DNA repair, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-5689603. UCH proteinases.
R-MMU-5696394. DNA Damage Recognition in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor protein YY1
Alternative name(s):
Delta transcription factor
NF-E1
UCR-motif DNA-binding protein
Yin and yang 1
Short name:
YY-1
Gene namesi
Name:Yy1
Synonyms:Ucrbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:99150. Yy1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Ino80 complex Source: MGI
  • nuclear chromatin Source: BHF-UCL
  • nuclear matrix Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • PcG protein complex Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Spermatocytes have a significant decrease in the global level of the heterochromatin markers and increase in the chromosomal double-strand break (DSB) signals at the leptotene/zygotene stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471911 – 414Transcriptional repressor protein YY1Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineBy similarity1
Cross-linki183Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei247PhosphoserineCombined sources1
Cross-linki286Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki288Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei378PhosphothreonineBy similarity1

Post-translational modificationi

Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ00899.
MaxQBiQ00899.
PaxDbiQ00899.
PeptideAtlasiQ00899.
PRIDEiQ00899.

PTM databases

iPTMnetiQ00899.
PhosphoSitePlusiQ00899.

Expressioni

Tissue specificityi

Expressed in ovary and, at lower levels, in testis.1 Publication

Developmental stagei

At 7.5 dpc, highly expressed in the ectoplacental cone and, at lower levels, in the embryonic and extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and yolk sac, and, at lower levels, in brain and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000021264.
CleanExiMM_YY1.
GenevisibleiQ00899. MM.

Interactioni

Subunit structurei

Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex (By similarity). Found in a complex with SMAD1 and SMAD4 (PubMed:15329343). Interacts with SFMBT2 (PubMed:18024232). Found in a complex with YY1, SIN3A and HDAC1 (PubMed:21454521).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Smc4Q8CG472EBI-6921536,EBI-6921575

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204624. 16 interactors.
DIPiDIP-59285N.
IntActiQ00899. 7 interactors.
MINTiMINT-4138987.
STRINGi10090.ENSMUSP00000021692.

Structurei

3D structure databases

ProteinModelPortaliQ00899.
SMRiQ00899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 170Interaction with the SMAD1/SMAD4 complexBy similarityAdd BLAST170
Regioni257 – 341Involved in nuclear matrix associationBy similarityAdd BLAST85
Regioni295 – 414Binding to DNABy similarityAdd BLAST120
Regioni333 – 371Involved in repression of activated transcriptionBy similarityAdd BLAST39
Regioni371 – 397Involved in masking transactivation domainBy similarityAdd BLAST27

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 53Asp/Glu-rich (acidic)Add BLAST11
Compositional biasi54 – 70Gly-richAdd BLAST17
Compositional biasi71 – 82Poly-HisAdd BLAST12
Compositional biasi161 – 170Gly/Ser-rich10

Sequence similaritiesi

Belongs to the YY transcription factor family.Curated
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri296 – 320C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri325 – 347C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri353 – 377C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri383 – 407C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiQ00899.
KOiK09201.
OMAiGGRKWEQ.
OrthoDBiEOG091G03FM.
PhylomeDBiQ00899.
TreeFamiTF106493.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
PIRSFiPIRSF037113. TF_Yin_yang. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGDTLYIA TDGSEMPAEI VELHEIEVET IPVETIETTV VGEEEEEDDD
60 70 80 90 100
DEDGGGGDHG GGGGGHGHAG HHHHHHHHHH HHPPMIALQP LVTDDPTQVH
110 120 130 140 150
HHQEVILVQT REEVVGGDDS DGLRAEDGFE DQILIPVPAP AGGDDDYIEQ
160 170 180 190 200
TLVTVAAAGK SGGGASSGGG RVKKGGGKKS GKKSYLGGGA GAAGGGGADP
210 220 230 240 250
GNKKWEQKQV QIKTLEGEFS VTMWSSDEKK DIDHETVVEE QIIGENSPPD
260 270 280 290 300
YSEYMTGKKL PPGGIPGIDL SDPKQLAEFA RMKPRKIKED DAPRTIACPH
310 320 330 340 350
KGCTKMFRDN SAMRKHLHTH GPRVHVCAEC GKAFVESSKL KRHQLVHTGE
360 370 380 390 400
KPFQCTFEGC GKRFSLDFNL RTHVRIHTGD RPYVCPFDGC NKKFAQSTNL
410
KSHILTHAKA KNNQ
Length:414
Mass (Da):44,717
Last modified:November 1, 1995 - v1
Checksum:iC012378288E984F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219F → S in AAA37521 (PubMed:1946404).Curated1
Sequence conflicti375R → G in AAA37521 (PubMed:1946404).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73963 mRNA. Translation: AAA40522.1.
L13968
, L13969, L13965, L13966, L13967 Genomic DNA. Translation: AAA40477.1.
M74590 mRNA. Translation: AAA37521.1.
BC055899 mRNA. Translation: AAH55899.1.
CCDSiCCDS26163.1.
PIRiA48273.
RefSeqiNP_033563.2. NM_009537.3.
UniGeneiMm.3868.
Mm.458511.

Genome annotation databases

EnsembliENSMUST00000021692; ENSMUSP00000021692; ENSMUSG00000021264.
GeneIDi22632.
KEGGimmu:22632.
UCSCiuc007pac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73963 mRNA. Translation: AAA40522.1.
L13968
, L13969, L13965, L13966, L13967 Genomic DNA. Translation: AAA40477.1.
M74590 mRNA. Translation: AAA37521.1.
BC055899 mRNA. Translation: AAH55899.1.
CCDSiCCDS26163.1.
PIRiA48273.
RefSeqiNP_033563.2. NM_009537.3.
UniGeneiMm.3868.
Mm.458511.

3D structure databases

ProteinModelPortaliQ00899.
SMRiQ00899.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204624. 16 interactors.
DIPiDIP-59285N.
IntActiQ00899. 7 interactors.
MINTiMINT-4138987.
STRINGi10090.ENSMUSP00000021692.

PTM databases

iPTMnetiQ00899.
PhosphoSitePlusiQ00899.

Proteomic databases

EPDiQ00899.
MaxQBiQ00899.
PaxDbiQ00899.
PeptideAtlasiQ00899.
PRIDEiQ00899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021692; ENSMUSP00000021692; ENSMUSG00000021264.
GeneIDi22632.
KEGGimmu:22632.
UCSCiuc007pac.1. mouse.

Organism-specific databases

CTDi7528.
MGIiMGI:99150. Yy1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiQ00899.
KOiK09201.
OMAiGGRKWEQ.
OrthoDBiEOG091G03FM.
PhylomeDBiQ00899.
TreeFamiTF106493.

Enzyme and pathway databases

ReactomeiR-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-5689603. UCH proteinases.
R-MMU-5696394. DNA Damage Recognition in GG-NER.

Miscellaneous databases

ChiTaRSiYy1. mouse.
PROiQ00899.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021264.
CleanExiMM_YY1.
GenevisibleiQ00899. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017114. TF_Yin_yang.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
PIRSFiPIRSF037113. TF_Yin_yang. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYY1_MOUSE
AccessioniPrimary (citable) accession number: Q00899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.