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Reviewed, UniProtKB/Swiss-Prot Q00891 (NPRM_BACME)

Last modified June 16, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bacillolysin
    EC=3.4.24.28
Alternative name(s):
    Neutral protease
Gene names
Name: nprM
OrganismBacillus megaterium
Taxonomic identifier1404 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

biophysicochemical properties

Temperature dependence:

Thermostable.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 245221Activation peptide Potential
PRO_0000028610
Chain246 – 562317Bacillolysin
PRO_0000028611

Sites

Active site3891 By similarity
Active site4771Proton donor By similarity
Metal binding3031Calcium 1 By similarity
Metal binding3051Calcium 1 By similarity
Metal binding3841Calcium 2 By similarity
Metal binding3881Zinc; catalytic By similarity
Metal binding3921Zinc; catalytic By similarity
Metal binding4121Zinc; catalytic By similarity
Metal binding4231Calcium 2 By similarity
Metal binding4231Calcium 3 By similarity
Metal binding4291Calcium 3; via carbonyl oxygen By similarity
Metal binding4311Calcium 2 By similarity
Metal binding4311Calcium 3 By similarity
Metal binding4331Calcium 2; via carbonyl oxygen By similarity
Metal binding4361Calcium 2 By similarity
Metal binding4361Calcium 3 By similarity
Metal binding4391Calcium 4; via carbonyl oxygen By similarity
Metal binding4401Calcium 4 By similarity
Metal binding4461Calcium 4 By similarity

Experimental info

Sequence conflict831S → N in CAA52964. Ref.2
Sequence conflict1861A → G in CAA52964. Ref.2
Sequence conflict2511T → A in CAA52964. Ref.2
Sequence conflict3021A → T in CAA52964. Ref.2
Sequence conflict3441R → A in CAA52964. Ref.2
Sequence conflict3941L → V in CAA52964. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q00891-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 17203441C7F6AAB7

FASTA56260,949
        10         20         30         40         50         60 
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI SGKLSGPSSQ 

        70         80         90        100        110        120 
KAQDVVFHYM NTNKDKYKLG NESAQNSFKV TEVVKDPVEQ ATVVRLQQVY NNIPVWGSTQ 

       130        140        150        160        170        180 
LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL 

       190        200        210        220        230        240 
YVYQNASDTT YAYVVNLNFL SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK 

       250        260        270        280        290        300 
PAAKPVTGTN TIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL 

       310        320        330        340        350        360 
WADTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGRPLKSTV HYSSGYNNAF 

       370        380        390        400        410        420 
WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THALTERSSN LIYQYESGAL NEAISDIFGT 

       430        440        450        460        470        480 
LVEYYDNRNP DWEIGEDIYT PGTSGDALRS MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS 

       490        500        510        520        530        540 
GIINKAAYLL ANGGTHYGVT VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD 

       550        560 
LYGSGSQEVI SVGKSFDAVG VQ

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References

[1]"Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581."
Kuhn S., Fortnagel P.
J. Gen. Microbiol. 139:39-47(1993) [PubMed: 8450307] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 14581 / DSM 32 / NCIB 9376 / NCTC 10342 / JCM 2506.
[2]"Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319."
Meinhardt F., Busskamp M., Wittchen K.D.
Appl. Microbiol. Biotechnol. 41:344-351(1994) [PubMed: 7764969] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 319.

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Cross-references

Sequence databases

X61380 Genomic DNA. Translation: CAA43654.1.
X75070 Genomic DNA. Translation: CAA52964.1.
PIRA47710.
I40227.

3D structure databases

HSSPHSSP built from PDB template 1NPC based on UniProtKB P05806.
SMRQ00891. Positions 246-561.
ModBaseSearch...

Protein family/group databases

MEROPSM04.001.

Enzyme and pathway databases

BRENDA3.4.24.28. 325.

Family and domain databases

InterProIPR005075. Pept_M4_propep_PepSY.
IPR006025. Pept_M_Zn_BS.
IPR013856. Peptidase_M4.
IPR001570. Peptidase_M4_C.
IPR011096. Propep_M4_M36.
[Graphical view]
Gene3DG3DSA:3.10.170.10. Peptidase_M4. 1 hit.
G3DSA:1.10.390.10. Peptidase_M4/M36. 1 hit.
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNPRM_BACME
AccessionPrimary (citable) accession number: Q00891
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents