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Q00859 (MAPK_FUSSO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase

EC=2.7.11.24
Alternative name(s):
FsMAPK
Gene names
Name:MAPK
OrganismFusarium solani subsp. pisi (Nectria haematococca)
Taxonomic identifier70791 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress by phosphorylating downstream targets By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Subcellular location

Nucleus Potential.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Mitogen-activated protein kinase
PRO_0000186335

Regions

Domain23 – 311289Protein kinase
Nucleotide binding29 – 379ATP By similarity
Motif183 – 1853TXY

Sites

Active site1471Proton acceptor By similarity
Binding site521ATP By similarity

Amino acid modifications

Modified residue1831Phosphothreonine By similarity
Modified residue1851Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00859 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D692EF98EF76C0CC

FASTA35541,221
        10         20         30         40         50         60 
MSRSNPPNPT GSRKISFNVS EQYDIQDVVG EGAYGVVCSA IHKPSGQKVA IKKITPFDHS 

        70         80         90        100        110        120 
MFCLRTLREM KLLRYFNHEN IISILDIQKP RNYESFNEVY LIQELMETDM HRAIRTQDLS 

       130        140        150        160        170        180 
DDHCQYFIYQ TLRALKAMHS ANVLHRDLKP SNLLLNANCD LKVCDFGLAR SAASQEDNSG 

       190        200        210        220        230        240 
FMTEYVATRW YRAPEIMLTF KEYTKAIDVW SVGCILAEML SGKPLFPGKD YHHQLTLILD 

       250        260        270        280        290        300 
VLGTPTMEDY YGIKSRRARE YIRSLPFKKK VPFRTLFPKT SDLALDLLEK LLAFNPVKRI 

       310        320        330        340        350 
TVEEALKHPY LEPYHDPEDE PTAPPIPEEF FDFDKHKDNL SKEQLKQLIY QEIMR 

« Hide

References

[1]"Cloning and expression of cDNA encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus."
Li D., Rogers L., Kolattukudy P.E.
Gene 195:161-166(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: T-8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52963 mRNA. Translation: AAB72017.1.

3D structure databases

ProteinModelPortalQ00859.
SMRQ00859. Positions 13-354.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ00859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 2361.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAPK_FUSSO
AccessionPrimary (citable) accession number: Q00859
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families