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Q00859

- MAPK_FUSSO

UniProt

Q00859 - MAPK_FUSSO

Protein

Mitogen-activated protein kinase

Gene

MAPK

Organism
Fusarium solani subsp. pisi (Nectria haematococca)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Responds to activation by environmental stress by phosphorylating downstream targets.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPPROSITE-ProRule annotation
    Active sitei147 – 1471Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 2361.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase (EC:2.7.11.24)
    Alternative name(s):
    FsMAPK
    Gene namesi
    Name:MAPK
    OrganismiFusarium solani subsp. pisi (Nectria haematococca)
    Taxonomic identifieri70791 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Mitogen-activated protein kinasePRO_0000186335Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphothreonineBy similarity
    Modified residuei185 – 1851PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ00859.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00859.
    SMRiQ00859. Positions 13-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 311289Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1853TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRSNPPNPT GSRKISFNVS EQYDIQDVVG EGAYGVVCSA IHKPSGQKVA    50
    IKKITPFDHS MFCLRTLREM KLLRYFNHEN IISILDIQKP RNYESFNEVY 100
    LIQELMETDM HRAIRTQDLS DDHCQYFIYQ TLRALKAMHS ANVLHRDLKP 150
    SNLLLNANCD LKVCDFGLAR SAASQEDNSG FMTEYVATRW YRAPEIMLTF 200
    KEYTKAIDVW SVGCILAEML SGKPLFPGKD YHHQLTLILD VLGTPTMEDY 250
    YGIKSRRARE YIRSLPFKKK VPFRTLFPKT SDLALDLLEK LLAFNPVKRI 300
    TVEEALKHPY LEPYHDPEDE PTAPPIPEEF FDFDKHKDNL SKEQLKQLIY 350
    QEIMR 355
    Length:355
    Mass (Da):41,221
    Last modified:November 1, 1996 - v1
    Checksum:iD692EF98EF76C0CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52963 mRNA. Translation: AAB72017.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52963 mRNA. Translation: AAB72017.1 .

    3D structure databases

    ProteinModelPortali Q00859.
    SMRi Q00859. Positions 13-354.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q00859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 2361.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cDNA encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus."
      Li D., Rogers L., Kolattukudy P.E.
      Gene 195:161-166(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: T-8.

    Entry informationi

    Entry nameiMAPK_FUSSO
    AccessioniPrimary (citable) accession number: Q00859
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3