Q00855 (ALL2_DERFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mite group 2 allergen Der f 2

Alternative name(s):
Allergen Der f II
Allergen=Der f 2

Gene names

Name:

DERF2

Organism

Dermatophagoides farinae (American house dust mite)

Taxonomic identifier

6954 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Acari › Acariformes › Sarcoptiformes › Astigmata › Psoroptidia › Analgoidea › Pyroglyphidae › Dermatophagoidinae › Dermatophagoides

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Allergenic properties	Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
Miscellaneous	The sequence shown here is from clone 2. The N-terminal sequence (AA 1-8) from clone 1 and 11 are not yet known.
Sequence similarities	Belongs to the NPC2 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Ref.4

Chain

18 – 146

129

Mite group 2 allergen Der f 2

PRO_0000019860

Amino acid modifications

Disulfide bond

25 ↔ 136

Ref.3

Disulfide bond

38 ↔ 44

Ref.3

Disulfide bond

90 ↔ 95

Ref.3

Natural variations

Natural variant

93

1

M → V in clone 1.

Natural variant

105

1

I → A in clone 11.

Natural variant

128

1

I → V in clone 11.

Natural variant

142

1

G → A in clone 11.

Experimental info

Sequence conflict

5 – 8

4

ILCL → GTMV in AAB30829. Ref.2

Secondary structure

1

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146

Helix Strand Turn

Details...

Beta strand

23 – 28

6

Beta strand

30 – 35

6

Beta strand

36 – 38

3

Beta strand

40 – 43

4

Beta strand

44 – 47

4

Beta strand

50 – 59

10

Beta strand

68 – 75

8

Beta strand

78 – 80

3

Beta strand

83 – 85

3

Helix

89 – 91

3

Turn

92 – 94

3

Beta strand

95 – 97

3

Beta strand

102 – 110

9

Beta strand

121 – 129

9

Beta strand

132 – 139

8

Beta strand

142 – 145

4

Sequences

	Sequence		Length	Mass (Da)
	Q00855 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FA118206CD88534A	FASTA	146	15,802
	10 20 30 40 50 60 MISKILCLSL LVAAVVADQV DVKDCANNEI KKVMVDGCHG SDPCIIHRGK PFTLEALFDA 70 80 90 100 110 120 NQNTKTAKIE IKASLDGLEI DVPGIDTNAC HFMKCPLVKG QQYDIKYTWN VPKIAPKSEN 130 140 VVVTVKLIGD NGVLACAIAT HGKIRD

References

[1]	"Cloning and expression of cDNA coding for the major house dust mite allergen Der f II in Escherichia coli." Yuuki T., Okumura Y., Ando T., Yamakawa H., Suko M., Haida M., Okudaira H. Agric. Biol. Chem. 55:1233-1238(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Molecular biology of mite antigens." Okuhira H. Arerugi 43:435-440(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-146.
[3]	"Determination of three disulfide bonds in a major house dust mite allergen, Der f II." Nishiyama C., Yuuki T., Takai T., Okumura Y., Okudaira H. Int. Arch. Allergy Immunol. 101:159-166(1993) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS, PARTIAL PROTEIN SEQUENCE.
[4]	"Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)." Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E. J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-52.
[5]	"Solution structure of Der f 2, the major mite allergen for atopic diseases." Ichikawa S., Hatanaka H., Yuuki T., Iwamoto N., Kojima S., Nishiyama C., Ogura K., Okumura Y., Inagaki F. J. Biol. Chem. 273:356-360(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D10447 mRNA. Translation: BAA01239.1.
D10448 mRNA. Translation: BAA01240.1.
D10449 mRNA. Translation: BAA01241.1.
S70378 mRNA. Translation: AAB30829.1.

PIR

JU0394.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AHK	NMR	-	A	18-146	[»]
1AHM	NMR	-	A	18-146	[»]
1WRF	NMR	-	A	18-146	[»]
1XWV	X-ray	1.83	A/B	18-146	[»]
2F08	X-ray	2.20	A/B/C/D	18-146	[»]

ProteinModelPortal

Q00855.

SMR

Q00855. Positions 18-146.

ModBase

Search...

Protein family/group databases

Allergome

1108. Der f 2.0103.
1294. Der f 2.0101.
1295. Der f 2.0102.
302. Der f 2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.40.770. 1 hit.

InterPro

IPR014756. Ig_E-set.
IPR003172. MD-2_lipid-recog_dom.
[Graphical view]

Pfam

PF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]

SMART

SM00737. ML. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q00855.

Entry information

Entry name

ALL2_DERFA

Accession

Primary (citable) accession number: Q00855
Secondary accession number(s): P39672, Q26359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)