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Reviewed, UniProtKB/Swiss-Prot Q00855 (ALL2_DERFA)

Last modified November 24, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Mite group 2 allergen Der f 2
Alternative name(s):
    Allergen Der f II
    Allergen=Der f 2
Gene names
Name: DERF2
OrganismDermatophagoides farinae (American house dust mite)
Taxonomic identifier6954 [NCBI]
Taxonomic lineageEukaryotaMetazoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Protein attributes

Sequence length146 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subcellular location

Secreted.

Allergenic properties

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Miscellaneous

The sequence shown here is from clone 2. The N-terminal sequence (AA 1-8) from clone 1 and 11 are not yet known.

Sequence similarities

Belongs to the NPC2 family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainSignal
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.4
Chain18 – 146129Mite group 2 allergen Der f 2
PRO_0000019860

Amino acid modifications

Disulfide bond25 ↔ 136 Ref.3
Disulfide bond38 ↔ 44 Ref.3
Disulfide bond90 ↔ 95 Ref.3

Natural variations

Natural variant931M → V in clone 1.
Natural variant1051I → A in clone 11.
Natural variant1281I → V in clone 11.
Natural variant1421G → A in clone 11.

Experimental info

Sequence conflict5 – 84ILCL → GTMV in AAB30829. Ref.2

Secondary structure

......................... 146
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00855-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: FA118206CD88534A

FASTA14615,802
        10         20         30         40         50         60 
MISKILCLSL LVAAVVADQV DVKDCANNEI KKVMVDGCHG SDPCIIHRGK PFTLEALFDA 

        70         80         90        100        110        120 
NQNTKTAKIE IKASLDGLEI DVPGIDTNAC HFMKCPLVKG QQYDIKYTWN VPKIAPKSEN 

       130        140 
VVVTVKLIGD NGVLACAIAT HGKIRD 

« Hide

References

[1]"Cloning and expression of cDNA coding for the major house dust mite allergen Der f II in Escherichia coli."
Yuuki T., Okumura Y., Ando T., Yamakawa H., Suko M., Haida M., Okudaira H.
Agric. Biol. Chem. 55:1233-1238(1991) [PubMed: 1368682] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Molecular biology of mite antigens."
Okuhira H.
Arerugi 43:435-440(1994) [PubMed: 8198452] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-146.
[3]"Determination of three disulfide bonds in a major house dust mite allergen, Der f II."
Nishiyama C., Yuuki T., Takai T., Okumura Y., Okudaira H.
Int. Arch. Allergy Immunol. 101:159-166(1993) [PubMed: 8508052] [Abstract]
Cited for: DISULFIDE BONDS, PARTIAL PROTEIN SEQUENCE.
[4]"Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)."
Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E.
J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed: 2732406] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 18-52.
[5]"Solution structure of Der f 2, the major mite allergen for atopic diseases."
Ichikawa S., Hatanaka H., Yuuki T., Iwamoto N., Kojima S., Nishiyama C., Ogura K., Okumura Y., Inagaki F.
J. Biol. Chem. 273:356-360(1998) [PubMed: 9417088] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10447 mRNA. Translation: BAA01239.1.
D10448 mRNA. Translation: BAA01240.1.
D10449 mRNA. Translation: BAA01241.1.
S70378 mRNA. Translation: AAB30829.1.
PIRJU0394.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AHKNMR-A18-146[»]
1AHMNMR-A18-146[»]
1WRFNMR-A18-146[»]
1XWVX-ray1.83A/B18-146[»]
2F08X-ray2.20A/B/C/D18-146[»]
ModBaseSearch...

Family and domain databases

InterProIPR014756. Ig_E-set.
IPR003172. MD-2_lipid-recog.
[Graphical view]
Gene3DG3DSA:2.60.40.770. MD-2_lipid-recog. 1 hit.
PfamPF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]
SMARTSM00737. ML. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALL2_DERFA
AccessionPrimary (citable) accession number: Q00855
Secondary accession number(s): P39672, Q26359
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Allergens

Nomenclature of allergens and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents