Q00816 (REG1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 120.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Resistance to glucose repression protein 1 Alternative name(s): Protein HEX2 Second-site suppressor of the rna1-1 mutation 1 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 1014 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in RNA processing and negative regulation of glucose repression. Regulates the level of two antigens, P43 and P70. Binds to protein phosphatase type 1. Functions with REG2 and SNF1 protein kinase to regulate growth. Might regulate SNF1 directly or indirectly. Ref.14 |
| Subunit structure | |
| Subcellular location | |
| Miscellaneous | Present with 2560 molecules/cell in log phase SD medium. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Nucleus |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | cellular response to glucose starvation Inferred from mutant phenotype PubMed 19723765. Source: SGD glycogen metabolic processTraceable author statement PubMed 10454550. Source: SGD negative regulation of transcription from RNA polymerase II promoterTraceable author statement PubMed 10454550. Source: SGD regulation of carbohydrate metabolic processInferred from genetic interaction PubMed 12167649. Source: SGD vacuolar protein catabolic processInferred from mutant phenotype PubMed 14684743. Source: SGD |
| Cellular_component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein phosphatase type 1 complexTraceable author statement PubMed 10454550. Source: SGD |
| Molecular_function | protein phosphatase type 1 regulator activity Traceable author statement PubMed 11412094. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| SNF1 | P06782 | 3 | EBI-8270,EBI-17516 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1014 | 1014 | Resistance to glucose repression protein 1 | PRO_0000083953 | |||||
Regions | |||||||||
| Motif | 277 – 283 | 7 | Nuclear localization signal Potential | ||||||
| Motif | 595 – 599 | 5 | Nuclear localization signal Potential | ||||||
| Motif | 873 – 879 | 7 | Nuclear localization signal Potential | ||||||
| Compositional bias | 543 – 552 | 10 | Ser-rich | ||||||
| Compositional bias | 742 – 760 | 19 | Asp/Glu-rich (acidic) | ||||||
| Compositional bias | 834 – 844 | 11 | Asp/Glu-rich (acidic) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 73 | 1 | Phosphothreonine Ref.11 | ||||||
| Modified residue | 75 | 1 | Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 | ||||||
| Modified residue | 242 | 1 | Phosphoserine Ref.12 Ref.13 | ||||||
| Modified residue | 254 | 1 | Phosphoserine Ref.12 Ref.13 | ||||||
| Modified residue | 311 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 349 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 421 | 1 | Phosphoserine Ref.10 Ref.12 Ref.13 | ||||||
| Modified residue | 480 | 1 | Phosphotyrosine Ref.11 | ||||||
| Modified residue | 485 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 490 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 570 | 1 | Phosphoserine Ref.7 Ref.12 Ref.13 | ||||||
| Modified residue | 572 | 1 | Phosphoserine Ref.10 Ref.12 Ref.13 | ||||||
| Modified residue | 576 | 1 | Phosphoserine Ref.7 Ref.10 Ref.12 Ref.13 | ||||||
| Modified residue | 582 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 586 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 587 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 608 | 1 | Phosphotyrosine Ref.13 | ||||||
| Modified residue | 610 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 614 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 680 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 767 | 1 | Phosphothreonine Ref.12 | ||||||
| Modified residue | 769 | 1 | Phosphoserine Ref.9 Ref.12 | ||||||
| Modified residue | 773 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 775 | 1 | Phosphoserine Ref.9 Ref.13 | ||||||
| Modified residue | 776 | 1 | Phosphothreonine Ref.7 Ref.13 | ||||||
| Modified residue | 778 | 1 | Phosphoserine Ref.7 Ref.13 | ||||||
| Modified residue | 886 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 898 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 925 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 977 | 1 | Phosphoserine Ref.12 Ref.13 | ||||||
| Modified residue | 1013 | 1 | Phosphoserine Ref.12 Ref.13 | ||||||
| Modified residue | 1014 | 1 | Phosphoserine Ref.12 Ref.13 | ||||||
Experimental info | |||||||||
| Sequence conflict | 376 – 377 | 2 | DK → EE in AAA34670. Ref.1 | ||||||
| Sequence conflict | 534 | 1 | N → K in AAA34670. Ref.1 | ||||||
| Sequence conflict | 657 | 1 | D → H in AAA34670. Ref.1 | ||||||
| Sequence conflict | 889 | 1 | S → T in AAA34670. Ref.1 | ||||||
| Sequence conflict | 988 – 1014 | 27 | ARGMA…GNDSS → QEVWQASTCTLGKRVTSSHK KMEMTAVRRKNFEVNMKRK Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeast." Niederacher D., Entian K.-D. Eur. J. Biochem. 200:311-319(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "SRN1, a yeast gene involved in RNA processing, is identical to HEX2/REG1, a negative regulator in glucose repression." Tung K.-S., Norbeck L.L., Nolan S.L., Atkinson N.S., Hopper A.K. Mol. Cell. Biol. 12:2673-2680(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P.Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames." Eide L.G., Sander C., Prydz H. Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 775-1014. |
| [6] | "REG1 binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae." Tu J.L., Carlson M. EMBO J. 14:5939-5946(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PP1. |
| [7] | "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570; SER-576; THR-776 AND SER-778, MASS SPECTROMETRY. Strain: 2124. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-75; SER-769 AND SER-775, MASS SPECTROMETRY. Strain: YAL6B. |
| [10] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-421; SER-572 AND SER-576, MASS SPECTROMETRY. Strain: ADR376. |
| [11] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-73; SER-75; TYR-480; SER-485 AND SER-490, MASS SPECTROMETRY. |
| [12] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-242; SER-254; SER-349; SER-421; SER-570; SER-572; SER-576; SER-586; SER-587; THR-767; SER-769; SER-977; SER-1013 AND SER-1014, MASS SPECTROMETRY. |
| [13] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-242; SER-254; SER-311; SER-421; SER-570; SER-572; SER-576; SER-582; TYR-608; SER-610; SER-614; SER-680; SER-773; SER-775; THR-776; SER-778; SER-886; SER-898; SER-925; SER-977; SER-1013 AND SER-1014, MASS SPECTROMETRY. |
| [14] | "Interaction of SNF1 protein kinase with its activating kinase Sak1." Liu Y., Xu X., Carlson M. Eukaryot. Cell 10:313-319(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SAK1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M33703 Genomic DNA. Translation: AAA34670.1. M90540 Genomic DNA. Translation: AAB59326.1. Z47814 Genomic DNA. Translation: CAA87807.1. Z74324 Genomic DNA. Translation: CAA98850.1. X95966 Genomic DNA. Translation: CAA65223.1. BK006938 Genomic DNA. Translation: DAA11873.1. |
| PIR | S32613. |
| RefSeq | NP_010311.1. NM_001180336.1. |
3D structure databases | |
| ProteinModelPortal | Q00816. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2513N. |
| IntAct | Q00816. 21 interactions. |
| MINT | MINT-648820. |
| STRING | 4932.YDR028C. |
Proteomic databases | |
| PaxDb | Q00816. |
| PeptideAtlas | Q00816. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YDR028C; YDR028C; YDR028C. |
| GeneID | 851592. |
| KEGG | sce:YDR028C. |
Organism-specific databases | |
| CYGD | YDR028c. |
| SGD | S000002435. REG1. |
Phylogenomic databases | |
| eggNOG | NOG70919. |
| OMA | WAKARNN. |
| OrthoDB | EOG4NPBC1. |
Gene expression databases | |
| Genevestigator | Q00816. |
| GermOnline | YDR028C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR013860. DUF1752_fun. [Graphical view] |
| Pfam | PF08550. DUF1752. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 969075. |
Entry information
| Entry name | REG1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q00816 Secondary accession number(s): D6VS13 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome IV Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names |

Clusters with
