Plasma membrane calcium-transporting ATPase 1 - Oryctolagus cuniculus (Rabbit)

Names and origin

Protein names

Recommended name:
    Plasma membrane calcium-transporting ATPase 1
      Short name=PMCA1
    EC=3.6.3.8
Alternative name(s):
    Plasma membrane calcium pump isoform 1

Gene names

Name:	ATP2B1
Synonyms:	PMCA1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

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Protein attributes

Sequence length	1249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Isoform B is ubiquitously expressed and is the most predominant isoform. Isoform C is expressed at much lower levels in all tissues tested, but liver, while isoform A is found only in aorta, brain and stomach.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform C (identifier: Q00804-1) Also known as: CIII; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q00804-2) Also known as: CII; The sequence of this isoform differs from the canonical sequence as follows: 1147-1249: IRVVNAFRSS...SPLHSLETSL → VTNISTPTHVVFSSSTASTTVGYSSGECIS

Isoform B (identifier: Q00804-3) Also known as: CI; The sequence of this isoform differs from the canonical sequence as follows: 1118-1146: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1249

1249

Plasma membrane calcium-transporting ATPase 1

PRO_0000046211

Regions

Topological domain

1 – 97

97

Cytoplasmic Potential

Transmembrane

98 – 118

21

Potential

Topological domain

119 – 154

36

Extracellular Potential

Transmembrane

155 – 175

21

Potential

Topological domain

176 – 366

191

Cytoplasmic Potential

Transmembrane

367 – 386

20

Potential

Topological domain

387 – 419

33

Extracellular Potential

Transmembrane

420 – 437

18

Potential

Topological domain

438 – 852

415

Cytoplasmic Potential

Transmembrane

853 – 872

20

Potential

Topological domain

873 – 882

10

Extracellular Potential

Transmembrane

883 – 903

21

Potential

Topological domain

904 – 923

20

Cytoplasmic Potential

Transmembrane

924 – 946

23

Potential

Topological domain

947 – 964

18

Extracellular Potential

Transmembrane

965 – 986

22

Potential

Topological domain

987 – 1005

19

Cytoplasmic Potential

Transmembrane

1006 – 1027

22

Potential

Topological domain

1028 – 1037

10

Extracellular Potential

Transmembrane

1038 – 1059

22

Potential

Topological domain

1060 – 1249

190

Cytoplasmic Potential

Region

1100 – 1117

18

Calmodulin-binding subdomain A By similarity

Region

1118 – 1127

10

Calmodulin-binding subdomain B By similarity

Compositional bias

296 – 299

4

Poly-Glu

Sites

Active site

475

1

4-aspartylphosphate intermediate

Metal binding

797

1

Magnesium By similarity

Metal binding

801

1

Magnesium By similarity

Amino acid modifications

Modified residue

17

1

Phosphoserine By similarity

Modified residue

1116

1

Phosphothreonine; by PKC By similarity

Modified residue

1184

1

Phosphoserine By similarity

Modified residue

1194

1

Phosphothreonine By similarity

Modified residue

1206

1

Phosphoserine; by PKA By similarity

Modified residue

1211

1

Phosphoserine By similarity

Modified residue

1237

1

Phosphoserine By similarity

Modified residue

1240

1

Phosphoserine By similarity

Modified residue

1244

1

Phosphoserine By similarity

Modified residue

1247

1

Phosphothreonine By similarity

Modified residue

1248

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1118 – 1146

29

Missing in isoform B.

VSP_000377

Alternative sequence

1147 – 1249

103

IRVVN…LETSL → VTNISTPTHVVFSSSTASTT VGYSSGECIS in isoform A.

VSP_000378

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Sequences

Sequence		Length	Mass (Da)
Isoform C (CIII) [UniParc]. Last modified June 1, 2001. Version 2. Checksum: D3EEAA2861621FEA Show »	FASTA	1,249	137,770
10 20 30 40 50 60 MGDMANNSVV YGGVKNSLKE ANHDGDFGIT LAAVRALMEL RSTDALRKIL ESYGDVYGIC 70 80 90 100 110 120 TKLKTSPNEG LRGNPADLER REAVFGKNFI PPKKPKTFLQ LVWEALQDVT LIILEIAAIV 130 140 150 160 170 180 SLGLSFYQPP EGDNALCGEV SVGEEEGEGE TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ 190 200 210 220 230 240 FRGLQSRIEQ EQKFTVIRGG QVIQIPVSDI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE 250 260 270 280 290 300 SSLTGESDHV KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK 310 320 330 340 350 360 KDEKKKEKKN KKQDGAIENR NKAKAQDGEP MEMQPLKSEE GGDGDEKDKK KANLPKKEKS 370 380 390 400 410 420 VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFLIDTFWVQ KRPWLAECTP IYIQYFVKFF 430 440 450 460 470 480 IIGVTVLVVA VPEGLPLPVT ISLAYSVNEM MKDNNLVRHL DACETMGNAT AICSDKTGTL 490 500 510 520 530 540 TMNRMAVVQA YINEKHYKKV PEPEPYPPNI LSYLVTGISV NCAYTSKILP PEEEGGLPRI 550 560 570 580 590 600 VGNKTECALL GPLLDLKQDY QDVRNEIPEE ALYKVYTFQS VRKSMSTVLK NSDGSFRIFS 610 620 630 640 650 660 KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL AFRDFPAGEP 670 680 690 700 710 720 EPEWDNENDI VTGLTCIAVV GIEDPGRPEV ADAIKKCQRA GITVEVVTGD NINTARAIAT 730 740 750 760 770 780 KCGILHPGED FLCLEGKDFN RRIRNEKGEI EQESIDKIWP KLRVLARSSP TDKHTLVKGI 790 800 810 820 830 840 IDSTVSEQRQ VVAVTGDGTN DGPALKKADG GFAMGIAGTD VAKEASDIIL TDDNFTSIVK 850 860 870 880 890 900 AVMWGRNVYD SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL 910 920 930 940 950 960 ALATEPPTES LLLGKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLLAG EKFFDIDSGR 970 980 990 1000 1010 1020 NAPLHAPPSE HYTIVFNIFV LMQLFNEINA RKIHGERNVF EGIFNNAIFC TIVLGTFVVQ 1030 1040 1050 1060 1070 1080 IIIVQFAGKP FSCSELSVEQ WLWSIFLGMG TLLWGQLIST IPTSRLKFQK EVVHGTQKRE 1090 1100 1110 1120 1130 1140 IGEEELAEDV EEIVHAEREL RRWQILWFRG LNRIQTQMDV VNAFQSGSSI QGALRRQPSI 1150 1160 1170 1180 1190 1200 ASQHHDIRVV NAFRSSLYEG LEKPESRSSI HNFMTHPEFR IEDSEPHIPL IDDTDAEDDA 1210 1220 1230 1240 PTKRNSSPPP SPNKNNNLVD SGIHLTIEMN KSATSSSPGS PLHSLETSL « Hide
Isoform A (CII). Checksum: 8F752C76121EBC9C Show »	FASTA	1,176	129,440
10 20 30 40 50 60 MGDMANNSVV YGGVKNSLKE ANHDGDFGIT LAAVRALMEL RSTDALRKIL ESYGDVYGIC 70 80 90 100 110 120 TKLKTSPNEG LRGNPADLER REAVFGKNFI PPKKPKTFLQ LVWEALQDVT LIILEIAAIV 130 140 150 160 170 180 SLGLSFYQPP EGDNALCGEV SVGEEEGEGE TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ 190 200 210 220 230 240 FRGLQSRIEQ EQKFTVIRGG QVIQIPVSDI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE 250 260 270 280 290 300 SSLTGESDHV KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK 310 320 330 340 350 360 KDEKKKEKKN KKQDGAIENR NKAKAQDGEP MEMQPLKSEE GGDGDEKDKK KANLPKKEKS 370 380 390 400 410 420 VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFLIDTFWVQ KRPWLAECTP IYIQYFVKFF 430 440 450 460 470 480 IIGVTVLVVA VPEGLPLPVT ISLAYSVNEM MKDNNLVRHL DACETMGNAT AICSDKTGTL 490 500 510 520 530 540 TMNRMAVVQA YINEKHYKKV PEPEPYPPNI LSYLVTGISV NCAYTSKILP PEEEGGLPRI 550 560 570 580 590 600 VGNKTECALL GPLLDLKQDY QDVRNEIPEE ALYKVYTFQS VRKSMSTVLK NSDGSFRIFS 610 620 630 640 650 660 KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL AFRDFPAGEP 670 680 690 700 710 720 EPEWDNENDI VTGLTCIAVV GIEDPGRPEV ADAIKKCQRA GITVEVVTGD NINTARAIAT 730 740 750 760 770 780 KCGILHPGED FLCLEGKDFN RRIRNEKGEI EQESIDKIWP KLRVLARSSP TDKHTLVKGI 790 800 810 820 830 840 IDSTVSEQRQ VVAVTGDGTN DGPALKKADG GFAMGIAGTD VAKEASDIIL TDDNFTSIVK 850 860 870 880 890 900 AVMWGRNVYD SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL 910 920 930 940 950 960 ALATEPPTES LLLGKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLLAG EKFFDIDSGR 970 980 990 1000 1010 1020 NAPLHAPPSE HYTIVFNIFV LMQLFNEINA RKIHGERNVF EGIFNNAIFC TIVLGTFVVQ 1030 1040 1050 1060 1070 1080 IIIVQFAGKP FSCSELSVEQ WLWSIFLGMG TLLWGQLIST IPTSRLKFQK EVVHGTQKRE 1090 1100 1110 1120 1130 1140 IGEEELAEDV EEIVHAEREL RRWQILWFRG LNRIQTQMDV VNAFQSGSSI QGALRRQPSI 1150 1160 1170 ASQHHDVTNI STPTHVVFSS STASTTVGYS SGECIS « Hide
Isoform B (CI). Checksum: 7E98CF428DC2CDD1 Show »	FASTA	1,220	134,651
10 20 30 40 50 60 MGDMANNSVV YGGVKNSLKE ANHDGDFGIT LAAVRALMEL RSTDALRKIL ESYGDVYGIC 70 80 90 100 110 120 TKLKTSPNEG LRGNPADLER REAVFGKNFI PPKKPKTFLQ LVWEALQDVT LIILEIAAIV 130 140 150 160 170 180 SLGLSFYQPP EGDNALCGEV SVGEEEGEGE TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ 190 200 210 220 230 240 FRGLQSRIEQ EQKFTVIRGG QVIQIPVSDI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE 250 260 270 280 290 300 SSLTGESDHV KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK 310 320 330 340 350 360 KDEKKKEKKN KKQDGAIENR NKAKAQDGEP MEMQPLKSEE GGDGDEKDKK KANLPKKEKS 370 380 390 400 410 420 VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFLIDTFWVQ KRPWLAECTP IYIQYFVKFF 430 440 450 460 470 480 IIGVTVLVVA VPEGLPLPVT ISLAYSVNEM MKDNNLVRHL DACETMGNAT AICSDKTGTL 490 500 510 520 530 540 TMNRMAVVQA YINEKHYKKV PEPEPYPPNI LSYLVTGISV NCAYTSKILP PEEEGGLPRI 550 560 570 580 590 600 VGNKTECALL GPLLDLKQDY QDVRNEIPEE ALYKVYTFQS VRKSMSTVLK NSDGSFRIFS 610 620 630 640 650 660 KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL AFRDFPAGEP 670 680 690 700 710 720 EPEWDNENDI VTGLTCIAVV GIEDPGRPEV ADAIKKCQRA GITVEVVTGD NINTARAIAT 730 740 750 760 770 780 KCGILHPGED FLCLEGKDFN RRIRNEKGEI EQESIDKIWP KLRVLARSSP TDKHTLVKGI 790 800 810 820 830 840 IDSTVSEQRQ VVAVTGDGTN DGPALKKADG GFAMGIAGTD VAKEASDIIL TDDNFTSIVK 850 860 870 880 890 900 AVMWGRNVYD SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL 910 920 930 940 950 960 ALATEPPTES LLLGKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLLAG EKFFDIDSGR 970 980 990 1000 1010 1020 NAPLHAPPSE HYTIVFNIFV LMQLFNEINA RKIHGERNVF EGIFNNAIFC TIVLGTFVVQ 1030 1040 1050 1060 1070 1080 IIIVQFAGKP FSCSELSVEQ WLWSIFLGMG TLLWGQLIST IPTSRLKFQK EVVHGTQKRE 1090 1100 1110 1120 1130 1140 IGEEELAEDV EEIVHAEREL RRWQILWFRG LNRIQTQIRV VNAFRSSLYE GLEKPESRSS 1150 1160 1170 1180 1190 1200 IHNFMTHPEF RIEDSEPHIP LIDDTDAEDD APTKRNSSPP PSPNKNNNLV DSGIHLTIEM 1210 1220 NKSATSSSPG SPLHSLETSL « Hide

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References

[1]

"Expression of cyclic-nucleotide-sensitive and -insensitive isoforms of the plasma membrane Ca2+ pump in smooth muscle and other tissues."
Khan I., Grover A.K.
Biochem. J. 277:345-349(1991) [PubMed: 1830473] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: Albino.
Tissue: Stomach smooth muscle.

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Cross-references

Sequence databases
	X59069 mRNA. Translation: CAA41792.1.
PIR	S17179.
RefSeq	NP_001095189.1.
UniGene	Ocu.3373
3D structure databases
HSSP	HSSP built from PDB template 1EUL based on UniProtKB P04191.
ModBase	Search...
Genome annotation databases
GeneID	100009543.
Organism-specific databases
CTD	100009543.
Phylogenomic databases
HOVERGEN	Q00804.
Enzyme and pathway databases
BRENDA	3.6.3.8. 255.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

AT2B1_RABIT

Accession

Primary (citable) accession number: Q00804

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	June 1, 2001
Last modified:	October 13, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents