SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q00780

- CO8A1_MOUSE

UniProt

Q00780 - CO8A1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Collagen alpha-1(VIII) chain
Gene
Col8a1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.3 Publications
Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis By similarity.3 Publications

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. camera-type eye morphogenesis Source: MGI
  3. cell adhesion Source: UniProtKB-KW
  4. epithelial cell proliferation Source: MGI
  5. positive regulation of cell-substrate adhesion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_199055. Collagen degradation.
REACT_216309. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(VIII) chain
Cleaved into the following chain:
Gene namesi
Name:Col8a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:88463. Col8a1.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: UniProtKB-SubCell
  2. collagen trimer Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

COL8A1-/COL8A2- mice exhibit decreased proliferation of measngial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 744720Collagen alpha-1(VIII) chain
PRO_0000005763Add
BLAST
Chaini573 – 744172Vastatin
PRO_0000390485Add
BLAST

Post-translational modificationi

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin By similarity.

Keywords - PTMi

Hydroxylation

Proteomic databases

PaxDbiQ00780.
PRIDEiQ00780.

PTM databases

PhosphoSiteiQ00780.

Expressioni

Tissue specificityi

High levels in calvarium, eye and skin of newborn mice; also in various epithelial, endothelial and mesenchymal cells.2 Publications

Inductioni

Up-regulated in astrocytes during the repair process and in mesangial cells in diabetic animals.2 Publications

Gene expression databases

BgeeiQ00780.
CleanExiMM_COL8A1.
GenevestigatoriQ00780.

Interactioni

Subunit structurei

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers By similarity.

Protein-protein interaction databases

BioGridi198827. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi617 – 6226
Beta strandi637 – 6426
Turni648 – 6503
Beta strandi659 – 68022
Beta strandi683 – 6908
Beta strandi698 – 70811
Beta strandi713 – 7175
Helixi721 – 7233
Beta strandi724 – 7274
Beta strandi734 – 74310

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O91X-ray1.90A/B/C573-744[»]
ProteinModelPortaliQ00780.
SMRiQ00780. Positions 614-744.

Miscellaneous databases

EvolutionaryTraceiQ00780.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini611 – 744134C1q
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 11890Nonhelical region (NC2)
Add
BLAST
Regioni119 – 572454Triple-helical region (COL1)
Add
BLAST
Regioni573 – 744172Nonhelical region (NC1)
Add
BLAST

Sequence similaritiesi

Contains 1 C1q domain.

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG114228.
GeneTreeiENSGT00750000117378.
HOGENOMiHOG000085653.
HOVERGENiHBG108220.
InParanoidiQ00780.
OMAiTCEVPGV.
OrthoDBiEOG70ZZPW.
PhylomeDBiQ00780.
TreeFamiTF334029.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00386. C1q. 1 hit.
PF01391. Collagen. 3 hits.
[Graphical view]
PRINTSiPR00007. COMPLEMNTC1Q.
SMARTiSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS50871. C1Q. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00780-1 [UniParc]FASTAAdd to Basket

« Hide

MAVPPRPLQL LGILFIISLN SVRLIQAGAY YGIKPLPPQI PPQIPPQIPQ    50
YQPLGQQVPH MPLGKDGLSM GKEMPHMQYG KEYPHLPQYM KEIPPVPRMG 100
KEVVPKKGKG EVPLASLRGE QGPRGEPGPR GPPGPPGLPG HGMPGIKGKP 150
GPQGYPGIGK PGMPGMPGKP GAMGMPGAKG EIGPKGEIGP MGIPGPQGPP 200
GPHGLPGIGK PGGPGLPGQP GAKGERGPKG PPGPPGLQGP KGEKGFGMPG 250
LPGLKGPPGM HGPPGPVGLP GVGKPGVTGF PGPQGPLGKP GPPGEPGPQG 300
LIGVPGVQGP PGMPGVGKPG QDGIPGQPGF PGGKGEQGLP GLPGPPGLPG 350
VGKPGFPGPK GDRGIGGVPG VLGPRGEKGP IGAPGMGGPP GEPGLPGIPG 400
PMGPPGAIGF PGPKGEGGVV GPQGPPGPKG EPGLQGFPGK PGFLGEVGPP 450
GMRGLPGPIG PKGEGGHKGL PGLPGVPGLL GPKGEPGIPG DQGLQGPPGI 500
PGIVGPSGPI GPPGIPGPKG EPGLPGPPGF PGVGKPGVAG LHGPPGKPGA 550
LGPQGQPGLP GPPGPPGPPG PPAVMPTPSP QGEYLPDMGL GIDGVKPPHA 600
YAGKKGKHGG PAYEMPAFTA ELTVPFPPVG APVKFDKLLY NGRQNYNPQT 650
GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPMMYTYD EYKKGFLDQA 700
SGSAVLLLRP GDQVFLQMPS EQAAGLYAGQ YVHSSFSGYL LYPM 744
Length:744
Mass (Da):73,607
Last modified:February 6, 2007 - v3
Checksum:i88F31CD20BDC8372
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31V → L in AAH11061. 1 Publication
Sequence conflicti6 – 61R → G in CAA47387. 1 Publication
Sequence conflicti85 – 851H → Y in CAA47387. 1 Publication
Sequence conflicti110 – 1101Missing in CAA47387. 1 Publication
Sequence conflicti249 – 2491P → L in CAA47387. 1 Publication
Sequence conflicti314 – 3141P → A in BAB31383. 1 Publication
Sequence conflicti324 – 3252IP → SR in CAA47387. 1 Publication
Sequence conflicti362 – 3621D → H in CAA47387. 1 Publication
Sequence conflicti597 – 5971P → T in CAA47387. 1 Publication
Sequence conflicti635 – 6351F → L in BAE35169. 1 Publication
Sequence conflicti718 – 7203MPS → NPF in CAA47387. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66976, X66977 Genomic DNA. Translation: CAA47387.1.
AK018742 mRNA. Translation: BAB31383.1.
AK032048 mRNA. Translation: BAC27670.1.
AK046370 mRNA. Translation: BAC32693.1.
AK159542 mRNA. Translation: BAE35169.1.
CT025753 Genomic DNA. Translation: CAX16095.1.
BC011061 mRNA. Translation: AAH11061.1.
CCDSiCCDS37367.1.
PIRiS23779.
RefSeqiNP_031765.2. NM_007739.2.
UniGeneiMm.130388.

Genome annotation databases

EnsembliENSMUST00000089332; ENSMUSP00000086745; ENSMUSG00000068196.
GeneIDi12837.
KEGGimmu:12837.
UCSCiuc007znl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66976 , X66977 Genomic DNA. Translation: CAA47387.1 .
AK018742 mRNA. Translation: BAB31383.1 .
AK032048 mRNA. Translation: BAC27670.1 .
AK046370 mRNA. Translation: BAC32693.1 .
AK159542 mRNA. Translation: BAE35169.1 .
CT025753 Genomic DNA. Translation: CAX16095.1 .
BC011061 mRNA. Translation: AAH11061.1 .
CCDSi CCDS37367.1.
PIRi S23779.
RefSeqi NP_031765.2. NM_007739.2.
UniGenei Mm.130388.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1O91 X-ray 1.90 A/B/C 573-744 [» ]
ProteinModelPortali Q00780.
SMRi Q00780. Positions 614-744.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198827. 1 interaction.

PTM databases

PhosphoSitei Q00780.

Proteomic databases

PaxDbi Q00780.
PRIDEi Q00780.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089332 ; ENSMUSP00000086745 ; ENSMUSG00000068196 .
GeneIDi 12837.
KEGGi mmu:12837.
UCSCi uc007znl.1. mouse.

Organism-specific databases

CTDi 1295.
MGIi MGI:88463. Col8a1.

Phylogenomic databases

eggNOGi NOG114228.
GeneTreei ENSGT00750000117378.
HOGENOMi HOG000085653.
HOVERGENi HBG108220.
InParanoidi Q00780.
OMAi TCEVPGV.
OrthoDBi EOG70ZZPW.
PhylomeDBi Q00780.
TreeFami TF334029.

Enzyme and pathway databases

Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_199055. Collagen degradation.
REACT_216309. Integrin cell surface interactions.

Miscellaneous databases

EvolutionaryTracei Q00780.
NextBioi 282360.
PROi Q00780.
SOURCEi Search...

Gene expression databases

Bgeei Q00780.
CleanExi MM_COL8A1.
Genevestigatori Q00780.

Family and domain databases

Gene3Di 2.60.120.40. 1 hit.
InterProi IPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view ]
Pfami PF00386. C1q. 1 hit.
PF01391. Collagen. 3 hits.
[Graphical view ]
PRINTSi PR00007. COMPLEMNTC1Q.
SMARTi SM00110. C1Q. 1 hit.
[Graphical view ]
SUPFAMi SSF49842. SSF49842. 1 hit.
PROSITEi PS50871. C1Q. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alpha 1(VIII)-collagen gene transcripts encode a short-chain collagen polypeptide and are expressed by various epithelial, endothelial and mesenchymal cells in newborn mouse tissues."
    Muragaki Y., Shiota C., Inoue M., Ooshima A., Olsen B.R., Ninomiya Y.
    Eur. J. Biochem. 207:895-902(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina, Kidney and Medulla oblongata.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Astrocytes express type VIII collagen during the repair process of brain cold injury."
    Hirano S., Yonezawa T., Hasegawa H., Hattori S., Greenhill N.S., Davis P.F., Sage E.H., Ninomiya Y.
    Biochem. Biophys. Res. Commun. 317:437-443(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, INDUCTION.
  6. "Migration and growth are attenuated in vascular smooth muscle cells with type VIII collagen-null alleles."
    Adiguzel E., Hou G., Mulholland D., Hopfer U., Fukai N., Olsen B., Bendeck M.
    Arterioscler. Thromb. Vasc. Biol. 26:56-61(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Lack of type VIII collagen in mice ameliorates diabetic nephropathy."
    Hopfer U., Hopfer H., Meyer-Schwesinger C., Loeffler I., Fukai N., Olsen B.R., Stahl R.A., Wolf G.
    Diabetes 58:1672-1681(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION, INDUCTION.
  8. "Crystal structure of the collagen alpha1(VIII) NC1 trimer."
    Kvansakul M., Bogin O., Hohenester E., Yayon A.
    Matrix Biol. 22:145-152(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 573-744.

Entry informationi

Entry nameiCO8A1_MOUSE
AccessioniPrimary (citable) accession number: Q00780
Secondary accession number(s): B8JJL9
, Q3TWU7, Q8BGL6, Q921S8, Q9D2V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi