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Protein

Collagen alpha-1(VIII) chain

Gene

Col8a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.3 Publications
Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.By similarity

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • camera-type eye morphogenesis Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • endodermal cell differentiation Source: Ensembl
  • epithelial cell proliferation Source: MGI
  • positive regulation of cell-substrate adhesion Source: MGI

Keywordsi

Biological processAngiogenesis, Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-216083 Integrin cell surface interactions
R-MMU-8948216 Collagen chain trimerization

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(VIII) chain
Cleaved into the following chain:
Gene namesi
Name:Col8a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:88463 Col8a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

COL8A1-/COL8A2- mice exhibit decreased proliferation of measngial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000576325 – 744Collagen alpha-1(VIII) chainAdd BLAST720
ChainiPRO_0000390485573 – 744VastatinAdd BLAST172

Post-translational modificationi

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin (By similarity).By similarity

Keywords - PTMi

Hydroxylation

Proteomic databases

MaxQBiQ00780
PaxDbiQ00780
PeptideAtlasiQ00780
PRIDEiQ00780

PTM databases

iPTMnetiQ00780
PhosphoSitePlusiQ00780

Expressioni

Tissue specificityi

High levels in calvarium, eye and skin of newborn mice; also in various epithelial, endothelial and mesenchymal cells.2 Publications

Inductioni

Up-regulated in astrocytes during the repair process and in mesangial cells in diabetic animals.2 Publications

Gene expression databases

BgeeiENSMUSG00000068196
CleanExiMM_COL8A1
GenevisibleiQ00780 MM

Interactioni

Subunit structurei

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrahedron stabilized by central interacting C-terminal NC1 trimers (By similarity).By similarity

Protein-protein interaction databases

BioGridi198827, 1 interactor
STRINGi10090.ENSMUSP00000086745

Structurei

Secondary structure

1744
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi617 – 622Combined sources6
Beta strandi637 – 642Combined sources6
Turni648 – 650Combined sources3
Beta strandi659 – 680Combined sources22
Beta strandi683 – 690Combined sources8
Beta strandi698 – 708Combined sources11
Beta strandi713 – 717Combined sources5
Helixi721 – 723Combined sources3
Beta strandi724 – 727Combined sources4
Beta strandi734 – 743Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O91X-ray1.90A/B/C573-744[»]
ProteinModelPortaliQ00780
SMRiQ00780
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00780

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini611 – 744C1qPROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 118Nonhelical region (NC2)Add BLAST90
Regioni119 – 572Triple-helical region (COL1)Add BLAST454
Regioni573 – 744Nonhelical region (NC1)Add BLAST172

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE8J Eukaryota
ENOG410Y928 LUCA
GeneTreeiENSGT00760000118830
HOGENOMiHOG000085653
HOVERGENiHBG108220
InParanoidiQ00780
OMAiTCEVPGV
OrthoDBiEOG091G0L3Y
PhylomeDBiQ00780
TreeFamiTF334029

Family and domain databases

Gene3Di2.60.120.40, 1 hit
InterProiView protein in InterPro
IPR001073 C1q_dom
IPR008160 Collagen
IPR008983 Tumour_necrosis_fac-like_dom
PfamiView protein in Pfam
PF00386 C1q, 1 hit
PF01391 Collagen, 3 hits
PRINTSiPR00007 COMPLEMNTC1Q
SMARTiView protein in SMART
SM00110 C1Q, 1 hit
SUPFAMiSSF49842 SSF49842, 1 hit
PROSITEiView protein in PROSITE
PS50871 C1Q, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPPRPLQL LGILFIISLN SVRLIQAGAY YGIKPLPPQI PPQIPPQIPQ
60 70 80 90 100
YQPLGQQVPH MPLGKDGLSM GKEMPHMQYG KEYPHLPQYM KEIPPVPRMG
110 120 130 140 150
KEVVPKKGKG EVPLASLRGE QGPRGEPGPR GPPGPPGLPG HGMPGIKGKP
160 170 180 190 200
GPQGYPGIGK PGMPGMPGKP GAMGMPGAKG EIGPKGEIGP MGIPGPQGPP
210 220 230 240 250
GPHGLPGIGK PGGPGLPGQP GAKGERGPKG PPGPPGLQGP KGEKGFGMPG
260 270 280 290 300
LPGLKGPPGM HGPPGPVGLP GVGKPGVTGF PGPQGPLGKP GPPGEPGPQG
310 320 330 340 350
LIGVPGVQGP PGMPGVGKPG QDGIPGQPGF PGGKGEQGLP GLPGPPGLPG
360 370 380 390 400
VGKPGFPGPK GDRGIGGVPG VLGPRGEKGP IGAPGMGGPP GEPGLPGIPG
410 420 430 440 450
PMGPPGAIGF PGPKGEGGVV GPQGPPGPKG EPGLQGFPGK PGFLGEVGPP
460 470 480 490 500
GMRGLPGPIG PKGEGGHKGL PGLPGVPGLL GPKGEPGIPG DQGLQGPPGI
510 520 530 540 550
PGIVGPSGPI GPPGIPGPKG EPGLPGPPGF PGVGKPGVAG LHGPPGKPGA
560 570 580 590 600
LGPQGQPGLP GPPGPPGPPG PPAVMPTPSP QGEYLPDMGL GIDGVKPPHA
610 620 630 640 650
YAGKKGKHGG PAYEMPAFTA ELTVPFPPVG APVKFDKLLY NGRQNYNPQT
660 670 680 690 700
GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPMMYTYD EYKKGFLDQA
710 720 730 740
SGSAVLLLRP GDQVFLQMPS EQAAGLYAGQ YVHSSFSGYL LYPM
Length:744
Mass (Da):73,607
Last modified:February 6, 2007 - v3
Checksum:i88F31CD20BDC8372
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3V → L in AAH11061 (PubMed:15489334).Curated1
Sequence conflicti6R → G in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti85H → Y in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti110Missing in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti249P → L in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti314P → A in BAB31383 (PubMed:16141072).Curated1
Sequence conflicti324 – 325IP → SR in CAA47387 (PubMed:1499564).Curated2
Sequence conflicti362D → H in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti597P → T in CAA47387 (PubMed:1499564).Curated1
Sequence conflicti635F → L in BAE35169 (PubMed:16141072).Curated1
Sequence conflicti718 – 720MPS → NPF in CAA47387 (PubMed:1499564).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66976, X66977 Genomic DNA Translation: CAA47387.1
AK018742 mRNA Translation: BAB31383.1
AK032048 mRNA Translation: BAC27670.1
AK046370 mRNA Translation: BAC32693.1
AK159542 mRNA Translation: BAE35169.1
CT025753 Genomic DNA Translation: CAX16095.1
BC011061 mRNA Translation: AAH11061.1
CCDSiCCDS37367.1
PIRiS23779
RefSeqiNP_031765.2, NM_007739.2
UniGeneiMm.130388

Genome annotation databases

EnsembliENSMUST00000089332; ENSMUSP00000086745; ENSMUSG00000068196
GeneIDi12837
KEGGimmu:12837
UCSCiuc007znl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCO8A1_MOUSE
AccessioniPrimary (citable) accession number: Q00780
Secondary accession number(s): B8JJL9
, Q3TWU7, Q8BGL6, Q921S8, Q9D2V4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 6, 2007
Last modified: May 23, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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