Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q00780

- CO8A1_MOUSE

UniProt

Q00780 - CO8A1_MOUSE

Protein

Collagen alpha-1(VIII) chain

Gene

Col8a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (06 Feb 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.3 Publications
    Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.By similarity

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. camera-type eye morphogenesis Source: MGI
    3. cell adhesion Source: UniProtKB-KW
    4. epithelial cell proliferation Source: MGI
    5. positive regulation of cell-substrate adhesion Source: MGI

    Keywords - Biological processi

    Angiogenesis, Cell adhesion

    Enzyme and pathway databases

    ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199046. Assembly of collagen fibrils and other multimeric structures.
    REACT_199055. Collagen degradation.
    REACT_216309. Integrin cell surface interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Collagen alpha-1(VIII) chain
    Cleaved into the following chain:
    Gene namesi
    Name:Col8a1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:88463. Col8a1.

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: UniProtKB-SubCell
    2. collagen trimer Source: UniProtKB-KW

    Keywords - Cellular componenti

    Basement membrane, Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    COL8A1-/COL8A2- mice exhibit decreased proliferation of measngial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 744720Collagen alpha-1(VIII) chainPRO_0000005763Add
    BLAST
    Chaini573 – 744172VastatinPRO_0000390485Add
    BLAST

    Post-translational modificationi

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin By similarity.By similarity

    Keywords - PTMi

    Hydroxylation

    Proteomic databases

    PaxDbiQ00780.
    PRIDEiQ00780.

    PTM databases

    PhosphoSiteiQ00780.

    Expressioni

    Tissue specificityi

    High levels in calvarium, eye and skin of newborn mice; also in various epithelial, endothelial and mesenchymal cells.2 Publications

    Inductioni

    Up-regulated in astrocytes during the repair process and in mesangial cells in diabetic animals.2 Publications

    Gene expression databases

    BgeeiQ00780.
    CleanExiMM_COL8A1.
    GenevestigatoriQ00780.

    Interactioni

    Subunit structurei

    Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198827. 1 interaction.

    Structurei

    Secondary structure

    1
    744
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi617 – 6226
    Beta strandi637 – 6426
    Turni648 – 6503
    Beta strandi659 – 68022
    Beta strandi683 – 6908
    Beta strandi698 – 70811
    Beta strandi713 – 7175
    Helixi721 – 7233
    Beta strandi724 – 7274
    Beta strandi734 – 74310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1O91X-ray1.90A/B/C573-744[»]
    ProteinModelPortaliQ00780.
    SMRiQ00780. Positions 614-744.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00780.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini611 – 744134C1qPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 11890Nonhelical region (NC2)Add
    BLAST
    Regioni119 – 572454Triple-helical region (COL1)Add
    BLAST
    Regioni573 – 744172Nonhelical region (NC1)Add
    BLAST

    Sequence similaritiesi

    Contains 1 C1q domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Collagen, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG114228.
    GeneTreeiENSGT00750000117378.
    HOGENOMiHOG000085653.
    HOVERGENiHBG108220.
    InParanoidiQ00780.
    OMAiTCEVPGV.
    OrthoDBiEOG70ZZPW.
    PhylomeDBiQ00780.
    TreeFamiTF334029.

    Family and domain databases

    Gene3Di2.60.120.40. 1 hit.
    InterProiIPR001073. C1q.
    IPR008160. Collagen.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view]
    PfamiPF00386. C1q. 1 hit.
    PF01391. Collagen. 3 hits.
    [Graphical view]
    PRINTSiPR00007. COMPLEMNTC1Q.
    SMARTiSM00110. C1Q. 1 hit.
    [Graphical view]
    SUPFAMiSSF49842. SSF49842. 1 hit.
    PROSITEiPS50871. C1Q. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00780-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPPRPLQL LGILFIISLN SVRLIQAGAY YGIKPLPPQI PPQIPPQIPQ    50
    YQPLGQQVPH MPLGKDGLSM GKEMPHMQYG KEYPHLPQYM KEIPPVPRMG 100
    KEVVPKKGKG EVPLASLRGE QGPRGEPGPR GPPGPPGLPG HGMPGIKGKP 150
    GPQGYPGIGK PGMPGMPGKP GAMGMPGAKG EIGPKGEIGP MGIPGPQGPP 200
    GPHGLPGIGK PGGPGLPGQP GAKGERGPKG PPGPPGLQGP KGEKGFGMPG 250
    LPGLKGPPGM HGPPGPVGLP GVGKPGVTGF PGPQGPLGKP GPPGEPGPQG 300
    LIGVPGVQGP PGMPGVGKPG QDGIPGQPGF PGGKGEQGLP GLPGPPGLPG 350
    VGKPGFPGPK GDRGIGGVPG VLGPRGEKGP IGAPGMGGPP GEPGLPGIPG 400
    PMGPPGAIGF PGPKGEGGVV GPQGPPGPKG EPGLQGFPGK PGFLGEVGPP 450
    GMRGLPGPIG PKGEGGHKGL PGLPGVPGLL GPKGEPGIPG DQGLQGPPGI 500
    PGIVGPSGPI GPPGIPGPKG EPGLPGPPGF PGVGKPGVAG LHGPPGKPGA 550
    LGPQGQPGLP GPPGPPGPPG PPAVMPTPSP QGEYLPDMGL GIDGVKPPHA 600
    YAGKKGKHGG PAYEMPAFTA ELTVPFPPVG APVKFDKLLY NGRQNYNPQT 650
    GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPMMYTYD EYKKGFLDQA 700
    SGSAVLLLRP GDQVFLQMPS EQAAGLYAGQ YVHSSFSGYL LYPM 744
    Length:744
    Mass (Da):73,607
    Last modified:February 6, 2007 - v3
    Checksum:i88F31CD20BDC8372
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31V → L in AAH11061. (PubMed:15489334)Curated
    Sequence conflicti6 – 61R → G in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti85 – 851H → Y in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti110 – 1101Missing in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti249 – 2491P → L in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti314 – 3141P → A in BAB31383. (PubMed:16141072)Curated
    Sequence conflicti324 – 3252IP → SR in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti362 – 3621D → H in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti597 – 5971P → T in CAA47387. (PubMed:1499564)Curated
    Sequence conflicti635 – 6351F → L in BAE35169. (PubMed:16141072)Curated
    Sequence conflicti718 – 7203MPS → NPF in CAA47387. (PubMed:1499564)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66976, X66977 Genomic DNA. Translation: CAA47387.1.
    AK018742 mRNA. Translation: BAB31383.1.
    AK032048 mRNA. Translation: BAC27670.1.
    AK046370 mRNA. Translation: BAC32693.1.
    AK159542 mRNA. Translation: BAE35169.1.
    CT025753 Genomic DNA. Translation: CAX16095.1.
    BC011061 mRNA. Translation: AAH11061.1.
    CCDSiCCDS37367.1.
    PIRiS23779.
    RefSeqiNP_031765.2. NM_007739.2.
    UniGeneiMm.130388.

    Genome annotation databases

    EnsembliENSMUST00000089332; ENSMUSP00000086745; ENSMUSG00000068196.
    GeneIDi12837.
    KEGGimmu:12837.
    UCSCiuc007znl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66976 , X66977 Genomic DNA. Translation: CAA47387.1 .
    AK018742 mRNA. Translation: BAB31383.1 .
    AK032048 mRNA. Translation: BAC27670.1 .
    AK046370 mRNA. Translation: BAC32693.1 .
    AK159542 mRNA. Translation: BAE35169.1 .
    CT025753 Genomic DNA. Translation: CAX16095.1 .
    BC011061 mRNA. Translation: AAH11061.1 .
    CCDSi CCDS37367.1.
    PIRi S23779.
    RefSeqi NP_031765.2. NM_007739.2.
    UniGenei Mm.130388.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1O91 X-ray 1.90 A/B/C 573-744 [» ]
    ProteinModelPortali Q00780.
    SMRi Q00780. Positions 614-744.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198827. 1 interaction.

    PTM databases

    PhosphoSitei Q00780.

    Proteomic databases

    PaxDbi Q00780.
    PRIDEi Q00780.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000089332 ; ENSMUSP00000086745 ; ENSMUSG00000068196 .
    GeneIDi 12837.
    KEGGi mmu:12837.
    UCSCi uc007znl.1. mouse.

    Organism-specific databases

    CTDi 1295.
    MGIi MGI:88463. Col8a1.

    Phylogenomic databases

    eggNOGi NOG114228.
    GeneTreei ENSGT00750000117378.
    HOGENOMi HOG000085653.
    HOVERGENi HBG108220.
    InParanoidi Q00780.
    OMAi TCEVPGV.
    OrthoDBi EOG70ZZPW.
    PhylomeDBi Q00780.
    TreeFami TF334029.

    Enzyme and pathway databases

    Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199046. Assembly of collagen fibrils and other multimeric structures.
    REACT_199055. Collagen degradation.
    REACT_216309. Integrin cell surface interactions.

    Miscellaneous databases

    EvolutionaryTracei Q00780.
    NextBioi 282360.
    PROi Q00780.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q00780.
    CleanExi MM_COL8A1.
    Genevestigatori Q00780.

    Family and domain databases

    Gene3Di 2.60.120.40. 1 hit.
    InterProi IPR001073. C1q.
    IPR008160. Collagen.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view ]
    Pfami PF00386. C1q. 1 hit.
    PF01391. Collagen. 3 hits.
    [Graphical view ]
    PRINTSi PR00007. COMPLEMNTC1Q.
    SMARTi SM00110. C1Q. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49842. SSF49842. 1 hit.
    PROSITEi PS50871. C1Q. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alpha 1(VIII)-collagen gene transcripts encode a short-chain collagen polypeptide and are expressed by various epithelial, endothelial and mesenchymal cells in newborn mouse tissues."
      Muragaki Y., Shiota C., Inoue M., Ooshima A., Olsen B.R., Ninomiya Y.
      Eur. J. Biochem. 207:895-902(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina, Kidney and Medulla oblongata.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Astrocytes express type VIII collagen during the repair process of brain cold injury."
      Hirano S., Yonezawa T., Hasegawa H., Hattori S., Greenhill N.S., Davis P.F., Sage E.H., Ninomiya Y.
      Biochem. Biophys. Res. Commun. 317:437-443(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION, INDUCTION.
    6. "Migration and growth are attenuated in vascular smooth muscle cells with type VIII collagen-null alleles."
      Adiguzel E., Hou G., Mulholland D., Hopfer U., Fukai N., Olsen B., Bendeck M.
      Arterioscler. Thromb. Vasc. Biol. 26:56-61(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Lack of type VIII collagen in mice ameliorates diabetic nephropathy."
      Hopfer U., Hopfer H., Meyer-Schwesinger C., Loeffler I., Fukai N., Olsen B.R., Stahl R.A., Wolf G.
      Diabetes 58:1672-1681(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION, INDUCTION.
    8. "Crystal structure of the collagen alpha1(VIII) NC1 trimer."
      Kvansakul M., Bogin O., Hohenester E., Yayon A.
      Matrix Biol. 22:145-152(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 573-744.

    Entry informationi

    Entry nameiCO8A1_MOUSE
    AccessioniPrimary (citable) accession number: Q00780
    Secondary accession number(s): B8JJL9
    , Q3TWU7, Q8BGL6, Q921S8, Q9D2V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3