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Q00780 (CO8A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen alpha-1(VIII) chain

Cleaved into the following chain:

  1. Vastatin
Gene names
Name:Col8a1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Ref.5 Ref.6 Ref.7

Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis By similarity. Ref.5 Ref.6 Ref.7

Subunit structure

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane.

Tissue specificity

High levels in calvarium, eye and skin of newborn mice; also in various epithelial, endothelial and mesenchymal cells. Ref.5 Ref.7

Induction

Up-regulated in astrocytes during the repair process and in mesangial cells in diabetic animals. Ref.5 Ref.7

Post-translational modification

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin By similarity.

Disruption phenotype

COL8A1-/COL8A2- mice exhibit decreased proliferation of measngial cells, reduced phosphorylation of ERK1/2 and increased p27(KIP1) expression. Diabetic COL8A1-/COL8A2- mice reveal reduced mesangial expansion and cellularity and extracellular matrix expansion. Ref.7

Sequence similarities

Contains 1 C1q domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 744720Collagen alpha-1(VIII) chain
PRO_0000005763
Chain573 – 744172Vastatin
PRO_0000390485

Regions

Domain611 – 744134C1q
Region29 – 11890Nonhelical region (NC2)
Region119 – 572454Triple-helical region (COL1)
Region573 – 744172Nonhelical region (NC1)

Experimental info

Sequence conflict31V → L in AAH11061. Ref.4
Sequence conflict61R → G in CAA47387. Ref.1
Sequence conflict851H → Y in CAA47387. Ref.1
Sequence conflict1101Missing in CAA47387. Ref.1
Sequence conflict2491P → L in CAA47387. Ref.1
Sequence conflict3141P → A in BAB31383. Ref.2
Sequence conflict324 – 3252IP → SR in CAA47387. Ref.1
Sequence conflict3621D → H in CAA47387. Ref.1
Sequence conflict5971P → T in CAA47387. Ref.1
Sequence conflict6351F → L in BAE35169. Ref.2
Sequence conflict718 – 7203MPS → NPF in CAA47387. Ref.1

Secondary structure

.................... 744
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00780 [UniParc].

Last modified February 6, 2007. Version 3.
Checksum: 88F31CD20BDC8372

FASTA74473,607
        10         20         30         40         50         60 
MAVPPRPLQL LGILFIISLN SVRLIQAGAY YGIKPLPPQI PPQIPPQIPQ YQPLGQQVPH 

        70         80         90        100        110        120 
MPLGKDGLSM GKEMPHMQYG KEYPHLPQYM KEIPPVPRMG KEVVPKKGKG EVPLASLRGE 

       130        140        150        160        170        180 
QGPRGEPGPR GPPGPPGLPG HGMPGIKGKP GPQGYPGIGK PGMPGMPGKP GAMGMPGAKG 

       190        200        210        220        230        240 
EIGPKGEIGP MGIPGPQGPP GPHGLPGIGK PGGPGLPGQP GAKGERGPKG PPGPPGLQGP 

       250        260        270        280        290        300 
KGEKGFGMPG LPGLKGPPGM HGPPGPVGLP GVGKPGVTGF PGPQGPLGKP GPPGEPGPQG 

       310        320        330        340        350        360 
LIGVPGVQGP PGMPGVGKPG QDGIPGQPGF PGGKGEQGLP GLPGPPGLPG VGKPGFPGPK 

       370        380        390        400        410        420 
GDRGIGGVPG VLGPRGEKGP IGAPGMGGPP GEPGLPGIPG PMGPPGAIGF PGPKGEGGVV 

       430        440        450        460        470        480 
GPQGPPGPKG EPGLQGFPGK PGFLGEVGPP GMRGLPGPIG PKGEGGHKGL PGLPGVPGLL 

       490        500        510        520        530        540 
GPKGEPGIPG DQGLQGPPGI PGIVGPSGPI GPPGIPGPKG EPGLPGPPGF PGVGKPGVAG 

       550        560        570        580        590        600 
LHGPPGKPGA LGPQGQPGLP GPPGPPGPPG PPAVMPTPSP QGEYLPDMGL GIDGVKPPHA 

       610        620        630        640        650        660 
YAGKKGKHGG PAYEMPAFTA ELTVPFPPVG APVKFDKLLY NGRQNYNPQT GIFTCEVPGV 

       670        680        690        700        710        720 
YYFAYHVHCK GGNVWVALFK NNEPMMYTYD EYKKGFLDQA SGSAVLLLRP GDQVFLQMPS 

       730        740 
EQAAGLYAGQ YVHSSFSGYL LYPM 

« Hide

References

« Hide 'large scale' references
[1]"Alpha 1(VIII)-collagen gene transcripts encode a short-chain collagen polypeptide and are expressed by various epithelial, endothelial and mesenchymal cells in newborn mouse tissues."
Muragaki Y., Shiota C., Inoue M., Ooshima A., Olsen B.R., Ninomiya Y.
Eur. J. Biochem. 207:895-902(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpora quadrigemina, Kidney and Medulla oblongata.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Astrocytes express type VIII collagen during the repair process of brain cold injury."
Hirano S., Yonezawa T., Hasegawa H., Hattori S., Greenhill N.S., Davis P.F., Sage E.H., Ninomiya Y.
Biochem. Biophys. Res. Commun. 317:437-443(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, FUNCTION, INDUCTION.
[6]"Migration and growth are attenuated in vascular smooth muscle cells with type VIII collagen-null alleles."
Adiguzel E., Hou G., Mulholland D., Hopfer U., Fukai N., Olsen B., Bendeck M.
Arterioscler. Thromb. Vasc. Biol. 26:56-61(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Lack of type VIII collagen in mice ameliorates diabetic nephropathy."
Hopfer U., Hopfer H., Meyer-Schwesinger C., Loeffler I., Fukai N., Olsen B.R., Stahl R.A., Wolf G.
Diabetes 58:1672-1681(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION, INDUCTION.
[8]"Crystal structure of the collagen alpha1(VIII) NC1 trimer."
Kvansakul M., Bogin O., Hohenester E., Yayon A.
Matrix Biol. 22:145-152(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 573-744.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66976, X66977 Genomic DNA. Translation: CAA47387.1.
AK018742 mRNA. Translation: BAB31383.1.
AK032048 mRNA. Translation: BAC27670.1.
AK046370 mRNA. Translation: BAC32693.1.
AK159542 mRNA. Translation: BAE35169.1.
CT025753 Genomic DNA. Translation: CAX16095.1.
BC011061 mRNA. Translation: AAH11061.1.
PIRS23779.
RefSeqNP_031765.2. NM_007739.2.
UniGeneMm.130388.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1O91X-ray1.90A/B/C573-744[»]
ProteinModelPortalQ00780.
SMRQ00780. Positions 614-744.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198827. 1 interaction.

PTM databases

PhosphoSiteQ00780.

Proteomic databases

PaxDbQ00780.
PRIDEQ00780.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000089332; ENSMUSP00000086745; ENSMUSG00000068196.
GeneID12837.
KEGGmmu:12837.
UCSCuc007znl.1. mouse.

Organism-specific databases

CTD1295.
MGIMGI:88463. Col8a1.

Phylogenomic databases

eggNOGNOG114228.
GeneTreeENSGT00750000117378.
HOGENOMHOG000085653.
HOVERGENHBG108220.
InParanoidQ00780.
OMATCEVPGV.
OrthoDBEOG70ZZPW.
PhylomeDBQ00780.
TreeFamTF334029.

Gene expression databases

BgeeQ00780.
CleanExMM_COL8A1.
GenevestigatorQ00780.

Family and domain databases

Gene3D2.60.120.40. 1 hit.
InterProIPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamPF00386. C1q. 1 hit.
PF01391. Collagen. 3 hits.
[Graphical view]
PRINTSPR00007. COMPLEMNTC1Q.
SMARTSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMSSF49842. SSF49842. 1 hit.
PROSITEPS50871. C1Q. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ00780.
NextBio282360.
PROQ00780.
SOURCESearch...

Entry information

Entry nameCO8A1_MOUSE
AccessionPrimary (citable) accession number: Q00780
Secondary accession number(s): B8JJL9 expand/collapse secondary AC list , Q3TWU7, Q8BGL6, Q921S8, Q9D2V4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot