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Q00776

- AP1M1_YEAST

UniProt

Q00776 - AP1M1_YEAST

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Protein

AP-1 complex subunit mu-1-I

Gene

APM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. AP57 is probably a subunit of the Golgi membrane adaptor.1 Publication

GO - Molecular functioni

  1. clathrin binding Source: SGD

GO - Biological processi

  1. Golgi to vacuole transport Source: SGD
  2. intracellular protein transport Source: InterPro
  3. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34144-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1-I
Alternative name(s):
Clathrin assembly protein complex 1 mu-1-I medium chain
Clathrin coat assembly protein AP54
Clathrin coat-associated protein AP54
Golgi adaptor AP-1 54 kDa protein
HA1 54 kDa subunit
Mu(1)-adaptin
Mu1-I-adaptin
Gene namesi
Name:APM1
Synonyms:YAP54
Ordered Locus Names:YPL259C
ORF Names:P0394
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL259c.
SGDiS000006180. APM1.

Subcellular locationi

GO - Cellular componenti

  1. AP-1 adaptor complex Source: SGD
  2. coated pit Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475AP-1 complex subunit mu-1-IPRO_0000193778Add
BLAST

Proteomic databases

MaxQBiQ00776.
PaxDbiQ00776.

Expressioni

Gene expression databases

GenevestigatoriQ00776.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
APL2P360008EBI-2624,EBI-2206
APS1P351814EBI-2624,EBI-2612

Protein-protein interaction databases

BioGridi35953. 79 interactions.
DIPiDIP-1198N.
IntActiQ00776. 13 interactions.
MINTiMINT-404390.
STRINGi4932.YPL259C.

Structurei

3D structure databases

ProteinModelPortaliQ00776.
SMRiQ00776. Positions 2-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 473299MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG315786.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
InParanoidiQ00776.
KOiK12393.
OMAiYPWVRYI.
OrthoDBiEOG76QFS9.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00776-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL
60 70 80 90 100
NHNGLEYLFI QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE
110 120 130 140 150
EESIRDNFVI IYELLDEVMD YGIPQITETK MLKQYITQKS FKLVKSAKKK
160 170 180 190 200
RNATRPPVAL TNSVSWRPEG ITHKKNEAFL DIVESINMLM TQKGQVLRSE
210 220 230 240 250
IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA SATTSDNNTE
260 270 280 290 300
TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK
310 320 330 340 350
FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE
360 370 380 390 400
ILIPVPDDAD TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG
410 420 430 440 450
LPSISNNEDG NRTMPKSNAE ILKGPVQIKF QIPYFTTSGI QVRYLKINEP
460 470
KLQYKSYPWV RYITQSGDDY TIRLT
Length:475
Mass (Da):53,874
Last modified:October 1, 1996 - v2
Checksum:iC6B11153B845921C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141M → I in CAA42828. (PubMed:1761056)Curated
Sequence conflicti216 – 2161D → H in CAA42828. (PubMed:1761056)Curated
Sequence conflicti222 – 2221N → K in CAA42828. (PubMed:1761056)Curated
Sequence conflicti433 – 4331P → R in CAA42828. (PubMed:1761056)Curated
Sequence conflicti440 – 4401I → M in CAA42828. (PubMed:1761056)Curated
Sequence conflicti450 – 4501Missing in CAA42828. (PubMed:1761056)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60288 Genomic DNA. Translation: CAA42828.1.
Z73615 Genomic DNA. Translation: CAA97989.1.
BK006949 Genomic DNA. Translation: DAA11176.1.
PIRiS65290.
RefSeqiNP_015064.1. NM_001184073.1.

Genome annotation databases

EnsemblFungiiYPL259C; YPL259C; YPL259C.
GeneIDi855869.
KEGGisce:YPL259C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60288 Genomic DNA. Translation: CAA42828.1 .
Z73615 Genomic DNA. Translation: CAA97989.1 .
BK006949 Genomic DNA. Translation: DAA11176.1 .
PIRi S65290.
RefSeqi NP_015064.1. NM_001184073.1.

3D structure databases

ProteinModelPortali Q00776.
SMRi Q00776. Positions 2-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35953. 79 interactions.
DIPi DIP-1198N.
IntActi Q00776. 13 interactions.
MINTi MINT-404390.
STRINGi 4932.YPL259C.

Proteomic databases

MaxQBi Q00776.
PaxDbi Q00776.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL259C ; YPL259C ; YPL259C .
GeneIDi 855869.
KEGGi sce:YPL259C.

Organism-specific databases

CYGDi YPL259c.
SGDi S000006180. APM1.

Phylogenomic databases

eggNOGi NOG315786.
GeneTreei ENSGT00530000062779.
HOGENOMi HOG000173247.
InParanoidi Q00776.
KOi K12393.
OMAi YPWVRYI.
OrthoDBi EOG76QFS9.

Enzyme and pathway databases

BioCyci YEAST:G3O-34144-MONOMER.

Miscellaneous databases

NextBioi 980499.

Gene expression databases

Genevestigatori Q00776.

Family and domain databases

InterProi IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view ]
Pfami PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view ]
PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
PRINTSi PR00314. CLATHRINADPT.
SUPFAMi SSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
    Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
    Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Adaptor complex-independent clathrin function in yeast."
    Yeung B.G., Phan H.L., Payne G.S.
    Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP1M1_YEAST
AccessioniPrimary (citable) accession number: Q00776
Secondary accession number(s): D6W3B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3