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Q00776

- AP1M1_YEAST

UniProt

Q00776 - AP1M1_YEAST

Protein

AP-1 complex subunit mu-1-I

Gene

APM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. AP57 is probably a subunit of the Golgi membrane adaptor.1 Publication

    GO - Molecular functioni

    1. clathrin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. Golgi to vacuole transport Source: SGD
    2. intracellular protein transport Source: InterPro
    3. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34144-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit mu-1-I
    Alternative name(s):
    Clathrin assembly protein complex 1 mu-1-I medium chain
    Clathrin coat assembly protein AP54
    Clathrin coat-associated protein AP54
    Golgi adaptor AP-1 54 kDa protein
    HA1 54 kDa subunit
    Mu(1)-adaptin
    Mu1-I-adaptin
    Gene namesi
    Name:APM1
    Synonyms:YAP54
    Ordered Locus Names:YPL259C
    ORF Names:P0394
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL259c.
    SGDiS000006180. APM1.

    Subcellular locationi

    GO - Cellular componenti

    1. AP-1 adaptor complex Source: SGD
    2. coated pit Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475AP-1 complex subunit mu-1-IPRO_0000193778Add
    BLAST

    Proteomic databases

    MaxQBiQ00776.
    PaxDbiQ00776.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00776.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APL2P360008EBI-2624,EBI-2206
    APS1P351814EBI-2624,EBI-2612

    Protein-protein interaction databases

    BioGridi35953. 78 interactions.
    DIPiDIP-1198N.
    IntActiQ00776. 13 interactions.
    MINTiMINT-404390.
    STRINGi4932.YPL259C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00776.
    SMRiQ00776. Positions 2-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini175 – 473299MHDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315786.
    GeneTreeiENSGT00530000062779.
    HOGENOMiHOG000173247.
    KOiK12393.
    OMAiYPWVRYI.
    OrthoDBiEOG76QFS9.

    Family and domain databases

    InterProiIPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view]
    PfamiPF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
    PRINTSiPR00314. CLATHRINADPT.
    SUPFAMiSSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00776-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL    50
    NHNGLEYLFI QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE 100
    EESIRDNFVI IYELLDEVMD YGIPQITETK MLKQYITQKS FKLVKSAKKK 150
    RNATRPPVAL TNSVSWRPEG ITHKKNEAFL DIVESINMLM TQKGQVLRSE 200
    IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA SATTSDNNTE 250
    TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK 300
    FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE 350
    ILIPVPDDAD TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG 400
    LPSISNNEDG NRTMPKSNAE ILKGPVQIKF QIPYFTTSGI QVRYLKINEP 450
    KLQYKSYPWV RYITQSGDDY TIRLT 475
    Length:475
    Mass (Da):53,874
    Last modified:October 1, 1996 - v2
    Checksum:iC6B11153B845921C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti214 – 2141M → I in CAA42828. (PubMed:1761056)Curated
    Sequence conflicti216 – 2161D → H in CAA42828. (PubMed:1761056)Curated
    Sequence conflicti222 – 2221N → K in CAA42828. (PubMed:1761056)Curated
    Sequence conflicti433 – 4331P → R in CAA42828. (PubMed:1761056)Curated
    Sequence conflicti440 – 4401I → M in CAA42828. (PubMed:1761056)Curated
    Sequence conflicti450 – 4501Missing in CAA42828. (PubMed:1761056)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60288 Genomic DNA. Translation: CAA42828.1.
    Z73615 Genomic DNA. Translation: CAA97989.1.
    BK006949 Genomic DNA. Translation: DAA11176.1.
    PIRiS65290.
    RefSeqiNP_015064.1. NM_001184073.1.

    Genome annotation databases

    EnsemblFungiiYPL259C; YPL259C; YPL259C.
    GeneIDi855869.
    KEGGisce:YPL259C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60288 Genomic DNA. Translation: CAA42828.1 .
    Z73615 Genomic DNA. Translation: CAA97989.1 .
    BK006949 Genomic DNA. Translation: DAA11176.1 .
    PIRi S65290.
    RefSeqi NP_015064.1. NM_001184073.1.

    3D structure databases

    ProteinModelPortali Q00776.
    SMRi Q00776. Positions 2-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35953. 78 interactions.
    DIPi DIP-1198N.
    IntActi Q00776. 13 interactions.
    MINTi MINT-404390.
    STRINGi 4932.YPL259C.

    Proteomic databases

    MaxQBi Q00776.
    PaxDbi Q00776.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL259C ; YPL259C ; YPL259C .
    GeneIDi 855869.
    KEGGi sce:YPL259C.

    Organism-specific databases

    CYGDi YPL259c.
    SGDi S000006180. APM1.

    Phylogenomic databases

    eggNOGi NOG315786.
    GeneTreei ENSGT00530000062779.
    HOGENOMi HOG000173247.
    KOi K12393.
    OMAi YPWVRYI.
    OrthoDBi EOG76QFS9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34144-MONOMER.

    Miscellaneous databases

    NextBioi 980499.

    Gene expression databases

    Genevestigatori Q00776.

    Family and domain databases

    InterProi IPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view ]
    Pfami PF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
    PRINTSi PR00314. CLATHRINADPT.
    SUPFAMi SSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
      Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
      Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Adaptor complex-independent clathrin function in yeast."
      Yeung B.G., Phan H.L., Payne G.S.
      Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP1M1_YEAST
    AccessioniPrimary (citable) accession number: Q00776
    Secondary accession number(s): D6W3B0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4420 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3