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Q00776 (AP1M1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit mu-1-I
Alternative name(s):
Clathrin assembly protein complex 1 mu-1-I medium chain
Clathrin coat assembly protein AP54
Clathrin coat-associated protein AP54
Golgi adaptor AP-1 54 kDa protein
HA1 54 kDa subunit
Mu(1)-adaptin
Mu1-I-adaptin
Gene names
Name:APM1
Synonyms:YAP54
Ordered Locus Names:YPL259C
ORF Names:P0394
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. AP57 is probably a subunit of the Golgi membrane adaptor. Ref.4

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin. Ref.4

Subcellular location

Cytoplasmic vesicle. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membraneclathrin-coated pit; Peripheral membrane protein; Cytoplasmic side By similarity.

Miscellaneous

Present with 4420 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APL2P360008EBI-2624,EBI-2206
APS1P351814EBI-2624,EBI-2612

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475AP-1 complex subunit mu-1-I
PRO_0000193778

Regions

Domain175 – 473299MHD

Experimental info

Sequence conflict2141M → I in CAA42828. Ref.1
Sequence conflict2161D → H in CAA42828. Ref.1
Sequence conflict2221N → K in CAA42828. Ref.1
Sequence conflict4331P → R in CAA42828. Ref.1
Sequence conflict4401I → M in CAA42828. Ref.1
Sequence conflict4501Missing in CAA42828. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00776 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: C6B11153B845921C

FASTA47553,874
        10         20         30         40         50         60 
MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL NHNGLEYLFI 

        70         80         90        100        110        120 
QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE EESIRDNFVI IYELLDEVMD 

       130        140        150        160        170        180 
YGIPQITETK MLKQYITQKS FKLVKSAKKK RNATRPPVAL TNSVSWRPEG ITHKKNEAFL 

       190        200        210        220        230        240 
DIVESINMLM TQKGQVLRSE IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA 

       250        260        270        280        290        300 
SATTSDNNTE TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK 

       310        320        330        340        350        360 
FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE ILIPVPDDAD 

       370        380        390        400        410        420 
TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG LPSISNNEDG NRTMPKSNAE 

       430        440        450        460        470 
ILKGPVQIKF QIPYFTTSGI QVRYLKINEP KLQYKSYPWV RYITQSGDDY TIRLT 

« Hide

References

« Hide 'large scale' references
[1]"The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast."
Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T.
Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Adaptor complex-independent clathrin function in yeast."
Yeung B.G., Phan H.L., Payne G.S.
Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60288 Genomic DNA. Translation: CAA42828.1.
Z73615 Genomic DNA. Translation: CAA97989.1.
BK006949 Genomic DNA. Translation: DAA11176.1.
PIRS65290.
RefSeqNP_015064.1. NM_001184073.1.

3D structure databases

ProteinModelPortalQ00776.
SMRQ00776. Positions 2-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35953. 77 interactions.
DIPDIP-1198N.
IntActQ00776. 13 interactions.
MINTMINT-404390.
STRING4932.YPL259C.

Proteomic databases

MaxQBQ00776.
PaxDbQ00776.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL259C; YPL259C; YPL259C.
GeneID855869.
KEGGsce:YPL259C.

Organism-specific databases

CYGDYPL259c.
SGDS000006180. APM1.

Phylogenomic databases

eggNOGNOG315786.
GeneTreeENSGT00530000062779.
HOGENOMHOG000173247.
KOK12393.
OMAYPWVRYI.
OrthoDBEOG76QFS9.

Enzyme and pathway databases

BioCycYEAST:G3O-34144-MONOMER.

Gene expression databases

GenevestigatorQ00776.

Family and domain databases

InterProIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
SUPFAMSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980499.

Entry information

Entry nameAP1M1_YEAST
AccessionPrimary (citable) accession number: Q00776
Secondary accession number(s): D6W3B0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families