AP-1 complex subunit mu-1-I - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q00776 (AP1M1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
AP-1 complex subunit mu-1-I

Alternative name(s):
Clathrin assembly protein complex 1 medium chain
Clathrin coat assembly protein AP54
Clathrin coat-associated protein AP54
Golgi adaptor AP-1 54 kDa protein
HA1 54 kDa subunit
Mu(1)-adaptin
Mu1-I-adaptin

Gene names

Name:	APM1
Synonyms:	YAP54
Ordered Locus Names:	YPL259C
ORF Names:	P0394

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. AP57 is probably a subunit of the Golgi membrane adaptor. Ref.4
Subunit structure	Assembly protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin. Ref.4
Subcellular location	Cytoplasmic vesicle. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membrane › clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side By similarity.
Miscellaneous	Present with 4420 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to vacuole transport Inferred from mutant phenotype PubMed 17003107. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	AP-1 adaptor complex Inferred from physical interaction Ref.4. Source: SGD coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	clathrin binding Inferred from sequence or structural similarity Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APL2	P36000	9	EBI-2624,EBI-2206
APS1	P35181	4	EBI-2624,EBI-2612

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 475

475

AP-1 complex subunit mu-1-I

PRO_0000193778

Regions

Domain

172 – 474

303

MHD

Experimental info

Sequence conflict

214

M → I in CAA42828. Ref.1

Sequence conflict

216

D → H in CAA42828. Ref.1

Sequence conflict

222

N → K in CAA42828. Ref.1

Sequence conflict

433

P → R in CAA42828. Ref.1

Sequence conflict

440

I → M in CAA42828. Ref.1

Sequence conflict

450

Missing in CAA42828. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q00776 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: C6B11153B845921C
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FASTA

475

53,874

        10         20         30         40         50         60 
MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL NHNGLEYLFI 

        70         80         90        100        110        120 
QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE EESIRDNFVI IYELLDEVMD 

       130        140        150        160        170        180 
YGIPQITETK MLKQYITQKS FKLVKSAKKK RNATRPPVAL TNSVSWRPEG ITHKKNEAFL 

       190        200        210        220        230        240 
DIVESINMLM TQKGQVLRSE IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA 

       250        260        270        280        290        300 
SATTSDNNTE TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK 

       310        320        330        340        350        360 
FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE ILIPVPDDAD 

       370        380        390        400        410        420 
TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG LPSISNNEDG NRTMPKSNAE 

       430        440        450        460        470 
ILKGPVQIKF QIPYFTTSGI QVRYLKINEP KLQYKSYPWV RYITQSGDDY TIRLT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast." Nakayama Y., Goebl M., O'Brine Greco B., Lemmon S., Pingchang C.E., Kirchhausen T. Eur. J. Biochem. 202:569-574(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60288 Genomic DNA. Translation: CAA42828.1. Z73615 Genomic DNA. Translation: CAA97989.1. BK006949 Genomic DNA. Translation: DAA11176.1.
PIR	S65290.
RefSeq	NP_015064.1. NM_001184073.1.
3D structure databases
ProteinModelPortal	Q00776.
SMR	Q00776. Positions 2-473.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1198N.
IntAct	Q00776. 30 interactions.
MINT	MINT-404390.
STRING	4932.YPL259C.
Proteomic databases
PaxDb	Q00776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL259C; YPL259C; YPL259C.
GeneID	855869.
KEGG	sce:YPL259C.
Organism-specific databases
CYGD	YPL259c.
SGD	S000006180. APM1.
Phylogenomic databases
eggNOG	NOG315786.
GeneTree	ENSGT00530000062779.
HOGENOM	HOG000173247.
KO	K12393.
OMA	IVGSVKM.
OrthoDB	EOG4NCQMT.
Gene expression databases
Genevestigator	Q00776.
GermOnline	YPL259C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022775. AP_mu_sigma_su. IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR011012. Longin-like_dom. [Graphical view]
Pfam	PF00928. Adap_comp_sub. 1 hit. PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
SUPFAM	SSF49447. AP50. 1 hit. SSF64356. Longin_like. 1 hit.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980499.

Entry information

Entry name

AP1M1_YEAST

Accession

Primary (citable) accession number: Q00776
Secondary accession number(s): D6W3B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents