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Protein

AP-1 complex subunit mu-1-I

Gene

APM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. AP57 is probably a subunit of the Golgi membrane adaptor.1 Publication

Miscellaneous

Present with 4420 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • clathrin binding Source: SGD

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • intracellular protein transport Source: InterPro
  • vesicle-mediated transport Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34144-MONOMER
ReactomeiR-SCE-432720 Lysosome Vesicle Biogenesis
R-SCE-6798695 Neutrophil degranulation
R-SCE-8856825 Cargo recognition for clathrin-mediated endocytosis
R-SCE-8866427 VLDLR internalisation and degradation
R-SCE-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1-I
Alternative name(s):
Clathrin assembly protein complex 1 mu-1-I medium chain
Clathrin coat assembly protein AP54
Clathrin coat-associated protein AP54
Golgi adaptor AP-1 54 kDa protein
HA1 54 kDa subunit
Mu(1)-adaptin
Mu1-I-adaptin
Gene namesi
Name:APM1
Synonyms:YAP54
Ordered Locus Names:YPL259C
ORF Names:P0394
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL259C
SGDiS000006180 APM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937781 – 475AP-1 complex subunit mu-1-IAdd BLAST475

Proteomic databases

MaxQBiQ00776
PaxDbiQ00776
PRIDEiQ00776

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi35953, 177 interactors
DIPiDIP-1198N
IntActiQ00776, 53 interactors
MINTiQ00776
STRINGi4932.YPL259C

Structurei

3D structure databases

ProteinModelPortaliQ00776
SMRiQ00776
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 473MHDPROSITE-ProRule annotationAdd BLAST299

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000062779
HOGENOMiHOG000173247
InParanoidiQ00776
KOiK12393
OMAiQQVTPCF
OrthoDBiEOG092C2NDI

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit

Sequencei

Sequence statusi: Complete.

Q00776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL
60 70 80 90 100
NHNGLEYLFI QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE
110 120 130 140 150
EESIRDNFVI IYELLDEVMD YGIPQITETK MLKQYITQKS FKLVKSAKKK
160 170 180 190 200
RNATRPPVAL TNSVSWRPEG ITHKKNEAFL DIVESINMLM TQKGQVLRSE
210 220 230 240 250
IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA SATTSDNNTE
260 270 280 290 300
TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK
310 320 330 340 350
FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE
360 370 380 390 400
ILIPVPDDAD TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG
410 420 430 440 450
LPSISNNEDG NRTMPKSNAE ILKGPVQIKF QIPYFTTSGI QVRYLKINEP
460 470
KLQYKSYPWV RYITQSGDDY TIRLT
Length:475
Mass (Da):53,874
Last modified:October 1, 1996 - v2
Checksum:iC6B11153B845921C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214M → I in CAA42828 (PubMed:1761056).Curated1
Sequence conflicti216D → H in CAA42828 (PubMed:1761056).Curated1
Sequence conflicti222N → K in CAA42828 (PubMed:1761056).Curated1
Sequence conflicti433P → R in CAA42828 (PubMed:1761056).Curated1
Sequence conflicti440I → M in CAA42828 (PubMed:1761056).Curated1
Sequence conflicti450Missing in CAA42828 (PubMed:1761056).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60288 Genomic DNA Translation: CAA42828.1
Z73615 Genomic DNA Translation: CAA97989.1
BK006949 Genomic DNA Translation: DAA11176.1
PIRiS65290
RefSeqiNP_015064.1, NM_001184073.1

Genome annotation databases

EnsemblFungiiYPL259C; YPL259C; YPL259C
GeneIDi855869
KEGGisce:YPL259C

Similar proteinsi

Entry informationi

Entry nameiAP1M1_YEAST
AccessioniPrimary (citable) accession number: Q00776
Secondary accession number(s): D6W3B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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