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Q00772

- SLT2_YEAST

UniProt

Q00772 - SLT2_YEAST

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Protein

Mitogen-activated protein kinase SLT2/MPK1

Gene

SLT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the SLT2/MPK1 kinase which itself then phosphorylates and activates the transcription factor RLM1. Directly phosphorylates BCY1 upon TOR complex 1 (TORC1) inhibition.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation by MKK1 and MKK2.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. barrier septum assembly Source: SGD
  2. endoplasmic reticulum unfolded protein response Source: SGD
  3. fungal-type cell wall biogenesis Source: SGD
  4. MAPK cascade Source: GOC
  5. peroxisome degradation Source: SGD
  6. protein phosphorylation Source: SGD
  7. regulation of cell size Source: SGD
  8. regulation of fungal-type cell wall organization Source: SGD
  9. regulation of transcription factor import into nucleus Source: SGD
  10. response to acidic pH Source: SGD
  11. signal transduction Source: SGD
  12. UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31090-MONOMER.
BRENDAi2.7.11.24. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase SLT2/MPK1 (EC:2.7.11.24)
Short name:
MAP kinase MPK1
Gene namesi
Name:SLT2
Synonyms:MPK1
Ordered Locus Names:YHR030C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR030c.
SGDiS000001072. SLT2.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. mating projection tip Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Mitogen-activated protein kinase SLT2/MPK1PRO_0000186338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901Phosphothreonine1 Publication
Modified residuei192 – 1921Phosphotyrosine1 Publication

Post-translational modificationi

Dually phosphorylated on Thr-190 and Tyr-192, which activates the enzyme.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ00772.
PaxDbiQ00772.
PeptideAtlasiQ00772.
PRIDEiQ00772.

Expressioni

Gene expression databases

GenevestigatoriQ00772.

Interactioni

Subunit structurei

Interacts with RLM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ADA2Q023362EBI-17372,EBI-2186
CDC73Q066972EBI-17372,EBI-29913
CTR9P891052EBI-17372,EBI-5283
GCN5Q033302EBI-17372,EBI-7458
HSP82P028294EBI-17372,EBI-8659
KIC1P386922EBI-17372,EBI-12253
LEO1P384392EBI-17372,EBI-10108
MKK2P324914EBI-17372,EBI-10973
PAF1P383512EBI-17372,EBI-12855
RTF1P530642EBI-17372,EBI-16303
SSK2P535992EBI-17372,EBI-18191
STE12P135742EBI-17372,EBI-18264

Protein-protein interaction databases

BioGridi36461. 621 interactions.
DIPiDIP-1448N.
IntActiQ00772. 39 interactions.
MINTiMINT-395773.
STRINGi4932.YHR030C.

Structurei

3D structure databases

ProteinModelPortaliQ00772.
SMRiQ00772. Positions 12-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 318296Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi190 – 1923TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 39122Poly-GlnAdd
BLAST

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000112267.
HOGENOMiHOG000233024.
InParanoidiQ00772.
KOiK04464.
OMAiFMPKKPF.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00772-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADKIERHTF KVFNQDFSVD KRFQLIKEIG HGAYGIVCSA RFAEAAEDTT
60 70 80 90 100
VAIKKVTNVF SKTLLCKRSL RELKLLRHFR GHKNITCLYD MDIVFYPDGS
110 120 130 140 150
INGLYLYEEL MECDMHQIIK SGQPLTDAHY QSFTYQILCG LKYIHSADVL
160 170 180 190 200
HRDLKPGNLL VNADCQLKIC DFGLARGYSE NPVENSQFLT EYVATRWYRA
210 220 230 240 250
PEIMLSYQGY TKAIDVWSAG CILAEFLGGK PIFKGKDYVN QLNQILQVLG
260 270 280 290 300
TPPDETLRRI GSKNVQDYIH QLGFIPKVPF VNLYPNANSQ ALDLLEQMLA
310 320 330 340 350
FDPQKRITVD EALEHPYLSI WHDPADEPVC SEKFEFSFES VNDMEDLKQM
360 370 380 390 400
VIQEVQDFRL FVRQPLLEEQ RQLQLQQQQQ QQQQQQQQQQ QPSDVDNGNA
410 420 430 440 450
AASEENYPKQ MATSNSVAPQ QESFGIHSQN LPRHDADFPP RPQESMMEMR
460 470 480
PATGNTADIP PQNDNGTLLD LEKELEFGLD RKYF
Length:484
Mass (Da):55,636
Last modified:February 1, 1995 - v2
Checksum:i559A3E0D3EBDE5F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561V → L in CAA41954. (PubMed:1779770)Curated
Sequence conflicti467 – 4671T → S in CAA41954. (PubMed:1779770)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59262 Genomic DNA. Translation: CAA41954.1.
U00062 Genomic DNA. Translation: AAB68912.1.
BK006934 Genomic DNA. Translation: DAA06721.1.
PIRiS43737.
RefSeqiNP_011895.1. NM_001179160.1.

Genome annotation databases

EnsemblFungiiYHR030C; YHR030C; YHR030C.
GeneIDi856425.
KEGGisce:YHR030C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59262 Genomic DNA. Translation: CAA41954.1 .
U00062 Genomic DNA. Translation: AAB68912.1 .
BK006934 Genomic DNA. Translation: DAA06721.1 .
PIRi S43737.
RefSeqi NP_011895.1. NM_001179160.1.

3D structure databases

ProteinModelPortali Q00772.
SMRi Q00772. Positions 12-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36461. 621 interactions.
DIPi DIP-1448N.
IntActi Q00772. 39 interactions.
MINTi MINT-395773.
STRINGi 4932.YHR030C.

Proteomic databases

MaxQBi Q00772.
PaxDbi Q00772.
PeptideAtlasi Q00772.
PRIDEi Q00772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR030C ; YHR030C ; YHR030C .
GeneIDi 856425.
KEGGi sce:YHR030C.

Organism-specific databases

CYGDi YHR030c.
SGDi S000001072. SLT2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000112267.
HOGENOMi HOG000233024.
InParanoidi Q00772.
KOi K04464.
OMAi FMPKKPF.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BioCyci YEAST:G3O-31090-MONOMER.
BRENDAi 2.7.11.24. 984.

Miscellaneous databases

NextBioi 982003.

Gene expression databases

Genevestigatori Q00772.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants."
    Torres L., Martin H., Garcia-Saez M.I., Arroyo J., Molina M., Sanchez M., Nombela C.
    Mol. Microbiol. 5:2845-2854(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae."
    Mazzoni C., Zarzov P., Rambourg A., Mann C.
    J. Cell Biol. 123:1821-1833(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway."
    Watanabe Y., Takaesu G., Hagiwara M., Irie K., Matsumoto K.
    Mol. Cell. Biol. 17:2615-2623(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RLM1.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates."
    Soulard A., Cremonesi A., Moes S., Schutz F., Jeno P., Hall M.N.
    Mol. Biol. Cell 21:3475-3486(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSLT2_YEAST
AccessioniPrimary (citable) accession number: Q00772
Secondary accession number(s): D3DKX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3