Mitogen-activated protein kinase SLT2/MPK1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q00772 (SLT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 133. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase SLT2/MPK1
Short name=MAP kinase MPK1
EC=2.7.11.24

Gene names

Name:	SLT2
Synonyms:	MPK1
Ordered Locus Names:	YHR030C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine protein kinase involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the SLT2/MPK1 kinase which itself then phosphorylates and activates the transcription factor RLM1. Directly phosphorylates BCY1 upon TOR complex 1 (TORC1) inhibition. Ref.5 Ref.10
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation by MKK1 and MKK2 Probable.
Subunit structure	Interacts with RLM1. Ref.5
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-190 and Tyr-192, which activates the enzyme.
Miscellaneous	Present with 3230 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response Inferred from mutant phenotype PubMed 18971375. Source: SGD barrier septum assembly Inferred from genetic interaction PubMed 19633265. Source: SGD fungal-type cell wall biogenesis Inferred from genetic interaction PubMed 16524906. Source: SGD peroxisome degradation Inferred from mutant phenotype PubMed 20385774. Source: SGD regulation of cell size Inferred from mutant phenotype PubMed 17302939. Source: SGD regulation of transcription factor import into nucleus Inferred from mutant phenotype PubMed 20219973. Source: SGD response to acid Inferred from mutant phenotype PubMed 16087742. Source: SGD
Cellular_component	cellular bud tip Inferred from direct assay PubMed 12361575. Source: SGD cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD nucleus Inferred from direct assay PubMed 12361575. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from sequence or structural similarity PubMed 8386319. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ADA2	Q02336	2	EBI-17372,EBI-2186
CDC73	Q06697	2	EBI-17372,EBI-29913
CTR9	P89105	2	EBI-17372,EBI-5283
GCN5	Q03330	2	EBI-17372,EBI-7458
HSP82	P02829	4	EBI-17372,EBI-8659
KIC1	P38692	3	EBI-17372,EBI-12253
LEO1	P38439	2	EBI-17372,EBI-10108
MKK2	P32491	5	EBI-17372,EBI-10973
PAF1	P38351	2	EBI-17372,EBI-12855
RTF1	P53064	2	EBI-17372,EBI-16303
SSK2	P53599	2	EBI-17372,EBI-18191
STE12	P13574	2	EBI-17372,EBI-18264

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 484

484

Mitogen-activated protein kinase SLT2/MPK1

PRO_0000186338

Regions

Domain

23 – 318

296

Protein kinase

Nucleotide binding

29 – 37

ATP By similarity

Motif

190 – 192

TXY

Compositional bias

370 – 391

Poly-Gln

Sites

Active site

153

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

190

Phosphothreonine Ref.6

Modified residue

192

Phosphotyrosine Ref.6 Ref.8

Modified residue

423

Phosphoserine Ref.9

Modified residue

428

Phosphoserine Ref.9

Experimental info

Sequence conflict

V → L in CAA41954. Ref.1

Sequence conflict

467

T → S in CAA41954. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q00772 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 559A3E0D3EBDE5F9
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FASTA

484

55,636

        10         20         30         40         50         60 
MADKIERHTF KVFNQDFSVD KRFQLIKEIG HGAYGIVCSA RFAEAAEDTT VAIKKVTNVF 

        70         80         90        100        110        120 
SKTLLCKRSL RELKLLRHFR GHKNITCLYD MDIVFYPDGS INGLYLYEEL MECDMHQIIK 

       130        140        150        160        170        180 
SGQPLTDAHY QSFTYQILCG LKYIHSADVL HRDLKPGNLL VNADCQLKIC DFGLARGYSE 

       190        200        210        220        230        240 
NPVENSQFLT EYVATRWYRA PEIMLSYQGY TKAIDVWSAG CILAEFLGGK PIFKGKDYVN 

       250        260        270        280        290        300 
QLNQILQVLG TPPDETLRRI GSKNVQDYIH QLGFIPKVPF VNLYPNANSQ ALDLLEQMLA 

       310        320        330        340        350        360 
FDPQKRITVD EALEHPYLSI WHDPADEPVC SEKFEFSFES VNDMEDLKQM VIQEVQDFRL 

       370        380        390        400        410        420 
FVRQPLLEEQ RQLQLQQQQQ QQQQQQQQQQ QPSDVDNGNA AASEENYPKQ MATSNSVAPQ 

       430        440        450        460        470        480 
QESFGIHSQN LPRHDADFPP RPQESMMEMR PATGNTADIP PQNDNGTLLD LEKELEFGLD 


RKYF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants." Torres L., Martin H., Garcia-Saez M.I., Arroyo J., Molina M., Sanchez M., Nombela C. Mol. Microbiol. 5:2845-2854(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae." Mazzoni C., Zarzov P., Rambourg A., Mann C. J. Cell Biol. 123:1821-1833(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway." Watanabe Y., Takaesu G., Hagiwara M., Irie K., Matsumoto K. Mol. Cell. Biol. 17:2615-2623(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RLM1.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-190 AND TYR-192, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-192, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423 AND SER-428, MASS SPECTROMETRY.
[10]	"The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates." Soulard A., Cremonesi A., Moes S., Schutz F., Jeno P., Hall M.N. Mol. Biol. Cell 21:3475-3486(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59262 Genomic DNA. Translation: CAA41954.1. U00062 Genomic DNA. Translation: AAB68912.1. BK006934 Genomic DNA. Translation: DAA06721.1.
PIR	S43737.
RefSeq	NP_011895.1. NM_001179160.1.
3D structure databases
ProteinModelPortal	Q00772.
SMR	Q00772. Positions 12-359.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1448N.
IntAct	Q00772. 65 interactions.
MINT	MINT-395773.
STRING	4932.YHR030C.
Proteomic databases
PaxDb	Q00772.
PeptideAtlas	Q00772.
PRIDE	Q00772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR030C; YHR030C; YHR030C.
GeneID	856425.
KEGG	sce:YHR030C.
Organism-specific databases
CYGD	YHR030c.
SGD	S000001072. SLT2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00690000102248.
HOGENOM	HOG000233024.
KO	K04464.
OMA	NSEHASG.
OrthoDB	EOG4S7NZG.
Enzyme and pathway databases
BRENDA	2.7.11.24. 984.
Gene expression databases
Genevestigator	Q00772.
GermOnline	YHR030C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982003.

Entry information

Entry name

SLT2_YEAST

Accession

Primary (citable) accession number: Q00772
Secondary accession number(s): D3DKX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 133 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents